Gene Symbol: NRPB3
Description: DNA-directed RNA polymerase family protein
Alias: F26H6.5, F26H6_5, NRPD3, NRPE3A, RBP36A, RPB35.5A, DNA-directed RNA polymerase family protein
Species: thale cress
Law J, Ausin I, Johnson L, Vashisht A, Zhu J, Wohlschlegel J, et al
. A protein complex required for polymerase V transcripts and RNA- directed DNA methylation in Arabidopsis. Curr Biol. 2010;20:951-6 pubmed publisher
..These results suggest that the DDR complex acts in RdDM at a step upstream of the recruitment or activation of Pol V. ..
Ream T, Haag J, Wierzbicki A, Nicora C, Norbeck A, Zhu J, et al
. Subunit compositions of the RNA-silencing enzymes Pol IV and Pol V reveal their origins as specialized forms of RNA polymerase II. Mol Cell. 2009;33:192-203 pubmed publisher
..Our findings support the hypothesis that Pol IV and Pol V are Pol II-like enzymes that evolved specialized roles in the production of noncoding transcripts for RNA silencing and genome defense. ..
Law J, Vashisht A, Wohlschlegel J, Jacobsen S. SHH1, a homeodomain protein required for DNA methylation, as well as RDR2, RDM4, and chromatin remodeling factors, associate with RNA polymerase IV. PLoS Genet. 2011;7:e1002195 pubmed publisher
Zhong X, Hale C, Nguyen M, Ausin I, Groth M, Hetzel J, et al
. Domains rearranged methyltransferase3 controls DNA methylation and regulates RNA polymerase V transcript abundance in Arabidopsis. Proc Natl Acad Sci U S A. 2015;112:911-6 pubmed publisher
..This work sheds further light on the mechanism by which long noncoding RNAs facilitate RNA-directed DNA methylation. ..
Zhang H, Ma Z, Zeng L, Tanaka K, Zhang C, Ma J, et al
. DTF1 is a core component of RNA-directed DNA methylation and may assist in the recruitment of Pol IV. Proc Natl Acad Sci U S A. 2013;110:8290-5 pubmed publisher
..Our results also suggest the involvement of DTF1 in an important negative feedback mechanism for DNA methylation at some RdDM target loci. ..
Sato H, Mizoi J, Tanaka H, Maruyama K, Qin F, Osakabe Y, et al
. Arabidopsis DPB3-1, a DREB2A interactor, specifically enhances heat stress-induced gene expression by forming a heat stress-specific transcriptional complex with NF-Y subunits. Plant Cell. 2014;26:4954-73 pubmed publisher
..We propose that the identified trimer contributes to the target gene selectivity of DREB2A under heat stress conditions. ..
Ulmasov T, Larkin R, Guilfoyle T. Association between 36- and 13.6-kDa alpha-like subunits of Arabidopsis thaliana RNA polymerase II. J Biol Chem. 1996;271:5085-94 pubmed
..6 subunits associate to form heterodimers in Arabidopsis RNA polymerase II like the AC40 and AC19 heterodimers reported for yeast RNA polymerases I and III but unlike the B44 homodimers reported for yeast RNA polymerase II. ..
Fukudome A, Aksoy E, Wu X, Kumar K, Jeong I, May K, et al
. Arabidopsis CPL4 is an essential C-terminal domain phosphatase that suppresses xenobiotic stress responses. Plant J. 2014;80:27-39 pubmed publisher
Chen L, Guan L, Qian P, Xu F, Wu Z, Wu Y, et al
. NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis. Development. 2016;143:1600-11 pubmed publisher
..we identify a partial loss-of-function mutation of the third largest subunit of nuclear DNA-dependent Pol II (NRPB3) that exhibits an increased number of stomatal lineage cells and paired stomata...