MPK6

Summary

Gene Symbol: MPK6
Description: MAP kinase 6
Alias: ATMAPK6, ATMPK6, F18O19.10, MAP KINASE 6, MAP kinase 6, MAPK6, MAP kinase 6
Species: thale cress
Products:     MPK6

Top Publications

  1. Ren D, Liu Y, Yang K, Han L, Mao G, Glazebrook J, et al. A fungal-responsive MAPK cascade regulates phytoalexin biosynthesis in Arabidopsis. Proc Natl Acad Sci U S A. 2008;105:5638-43 pubmed publisher
    Plant recognition of pathogens leads to rapid activation of MPK3 and MPK6, two Arabidopsis mitogen-activated protein kinases (MAPKs), and their orthologs in other species...
  2. Xing Y, Jia W, Zhang J. AtMKK1 mediates ABA-induced CAT1 expression and H2O2 production via AtMPK6-coupled signaling in Arabidopsis. Plant J. 2008;54:440-51 pubmed publisher
    ..b>AtMPK6, another component in the MAPK cascade, was also involved: mpk6 mutant blocked and overexpressing AtMPK6 enhanced ..
  3. Schweighofer A, Kazanaviciute V, Scheikl E, Teige M, Doczi R, Hirt H, et al. The PP2C-type phosphatase AP2C1, which negatively regulates MPK4 and MPK6, modulates innate immunity, jasmonic acid, and ethylene levels in Arabidopsis. Plant Cell. 2007;19:2213-24 pubmed
    ..phosphatase of type 2C, is a novel stress signal regulator that inactivates the stress-responsive MAPKs MPK4 and MPK6. Mutant ap2c1 plants produce significantly higher amounts of jasmonate upon wounding and are more resistant to ..
  4. Tsugama D, Liu S, Takano T. Drought-induced activation and rehydration-induced inactivation of MPK6 in Arabidopsis. Biochem Biophys Res Commun. 2012;426:626-9 pubmed publisher
    ..Here we show that the activation state of one of the Arabidopsis MPKs, MPK6, is directly regulated by drought and rehydration...
  5. Hord C, Sun Y, Pillitteri L, Torii K, Wang H, Zhang S, et al. Regulation of Arabidopsis early anther development by the mitogen-activated protein kinases, MPK3 and MPK6, and the ERECTA and related receptor-like kinases. Mol Plant. 2008;1:645-58 pubmed publisher
    ..Two closely related genes encoding MAPKs, MPK3 and MPK6, function redundantly in regulating stomatal patterning...
  6. Li G, Meng X, Wang R, Mao G, Han L, Liu Y, et al. Dual-level regulation of ACC synthase activity by MPK3/MPK6 cascade and its downstream WRKY transcription factor during ethylene induction in Arabidopsis. PLoS Genet. 2012;8:e1002767 pubmed publisher
    ..in Botrytis cinerea-induced ethylene biosynthesis and their regulation at the protein stability level by MPK3 and MPK6, two Arabidopsis pathogen-responsive mitogen-activated protein kinases (MAPKs)...
  7. Sörensson C, Lenman M, Veide Vilg J, Schopper S, Ljungdahl T, Grøtli M, et al. Determination of primary sequence specificity of Arabidopsis MAPKs MPK3 and MPK6 leads to identification of new substrates. Biochem J. 2012;446:271-8 pubmed publisher
    ..random positional peptide library to search for consensus phosphorylation sequences for Arabidopsis MAPKs MPK3 and MPK6. These experiments indicated a preference towards the sequence L/P-P/X-S-P-R/K for both kinases...
  8. Merkouropoulos G, Andreasson E, Hess D, Boller T, Peck S. An Arabidopsis protein phosphorylated in response to microbial elicitation, AtPHOS32, is a substrate of MAP kinases 3 and 6. J Biol Chem. 2008;283:10493-9 pubmed publisher
    ..Using in vitro kinase assays, we confirmed that AtPHOS32 is a substrate of both AtMPK3 and AtMPK6. Specificity of phosphorylation was demonstrated by site-directed mutagenesis of the first phosphorylation site...
  9. Wang H, Ngwenyama N, Liu Y, Walker J, Zhang S. Stomatal development and patterning are regulated by environmentally responsive mitogen-activated protein kinases in Arabidopsis. Plant Cell. 2007;19:63-73 pubmed
    ..Here, we report that Arabidopsis MITOGEN-ACTIVATED PROTEIN KINASE3 (MPK3) and MPK6, two environmentally responsive mitogen-activated protein kinases (MAPKs), and their upstream MAPK kinases, MKK4 ..

More Information

Publications79

  1. Han L, Li G, Yang K, Mao G, Wang R, Liu Y, et al. Mitogen-activated protein kinase 3 and 6 regulate Botrytis cinerea-induced ethylene production in Arabidopsis. Plant J. 2010;64:114-27 pubmed publisher
    ..b>MPK6, an Arabidopsis stress-responsive mitogen-activated protein kinase (MAPK) was previously shown to regulate the ..
  2. Brock A, Willmann R, Kolb D, Grefen L, Lajunen H, Bethke G, et al. The Arabidopsis mitogen-activated protein kinase phosphatase PP2C5 affects seed germination, stomatal aperture, and abscisic acid-inducible gene expression. Plant Physiol. 2010;153:1098-111 pubmed publisher
    ..The PP2C5 protein colocalizes and directly interacts with stress-induced MPK3, MPK4, and MPK6, predominantly in the nucleus. Importantly, altered PP2C5 levels affect MAPK activation...
  3. Kohorn B, Kohorn S, Todorova T, Baptiste G, Stansky K, McCullough M. A dominant allele of Arabidopsis pectin-binding wall-associated kinase induces a stress response suppressed by MPK6 but not MPK3 mutations. Mol Plant. 2012;5:841-51 pubmed publisher
    ..link between cell surface receptors and both transcriptional and enzyme regulation in eukaryotes, and Arabidopsis MPK6 and MPK3 indeed have important roles in development and the response to stress and pathogens...
  4. Wang P, Du Y, Li Y, Ren D, Song C. Hydrogen peroxide-mediated activation of MAP kinase 6 modulates nitric oxide biosynthesis and signal transduction in Arabidopsis. Plant Cell. 2010;22:2981-98 pubmed publisher
    ..and genetic approaches to analyze the function of Arabidopsis thaliana mitogen-activated protein kinase 6 (MPK6) in the regulation of NO synthesis in response to hydrogen peroxide (H?O?) during lateral root development...
  5. Liu Y, Zhang S. Phosphorylation of 1-aminocyclopropane-1-carboxylic acid synthase by MPK6, a stress-responsive mitogen-activated protein kinase, induces ethylene biosynthesis in Arabidopsis. Plant Cell. 2004;16:3386-99 pubmed
    ..Here, we report that MPK6, the Arabidopsis thaliana ortholog of tobacco SIPK, is required for ethylene induction in this transgenic system...
  6. Asai T, Tena G, Plotnikova J, Willmann M, Chiu W, Gomez Gomez L, et al. MAP kinase signalling cascade in Arabidopsis innate immunity. Nature. 2002;415:977-83 pubmed
    ..Here we identify a complete plant MAP kinase cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) and WRKY22/WRKY29 transcription factors that function downstream of the flagellin receptor FLS2, a leucine-rich-..
  7. Meng X, Xu J, He Y, Yang K, Mordorski B, Liu Y, et al. Phosphorylation of an ERF transcription factor by Arabidopsis MPK3/MPK6 regulates plant defense gene induction and fungal resistance. Plant Cell. 2013;25:1126-42 pubmed publisher
    Arabidopsis thaliana MPK3 and MPK6, two mitogen-activated protein kinases (MAPKs or MPKs), play critical roles in plant disease resistance by regulating multiple defense responses...
  8. Muller J, Beck M, Mettbach U, Komis G, Hause G, Menzel D, et al. Arabidopsis MPK6 is involved in cell division plane control during early root development, and localizes to the pre-prophase band, phragmoplast, trans-Golgi network and plasma membrane. Plant J. 2010;61:234-48 pubmed publisher
    ..Here, we analysed expression, subcellular localization and function of MPK6 in roots of Arabidopsis thaliana using wild-type plants and three mpk6 knock-out mutant lines...
  9. Bethke G, Unthan T, Uhrig J, Pöschl Y, Gust A, Scheel D, et al. Flg22 regulates the release of an ethylene response factor substrate from MAP kinase 6 in Arabidopsis thaliana via ethylene signaling. Proc Natl Acad Sci U S A. 2009;106:8067-72 pubmed publisher
    ..Here, the in vivo enzyme-substrate interaction of the Arabidopsis thaliana MAP kinase, MPK6, with an ethylene response factor (ERF104) is shown by fluorescence resonance energy transfer...
  10. Lee J, Ellis B. Arabidopsis MAPK phosphatase 2 (MKP2) positively regulates oxidative stress tolerance and inactivates the MPK3 and MPK6 MAPKs. J Biol Chem. 2007;282:25020-9 pubmed
    Two closely related Arabidopsis mitogen-activated protein kinases (MAPKs), MPK3 and MPK6, are rapidly but transiently activated in plants exposed to ozone...
  11. Wang H, Liu Y, Bruffett K, Lee J, Hause G, Walker J, et al. Haplo-insufficiency of MPK3 in MPK6 mutant background uncovers a novel function of these two MAPKs in Arabidopsis ovule development. Plant Cell. 2008;20:602-13 pubmed publisher
    ..Here, we report that two closely related mitogen-activated protein kinases in Arabidopsis thaliana, MPK3 and MPK6, share a novel function in ovule development: in the MPK6 mutant background, MPK3 is haplo-insufficient, giving ..
  12. Takahashi F, Yoshida R, Ichimura K, Mizoguchi T, Seo S, Yonezawa M, et al. The mitogen-activated protein kinase cascade MKK3-MPK6 is an important part of the jasmonate signal transduction pathway in Arabidopsis. Plant Cell. 2007;19:805-18 pubmed
    ..Here, we identify a mitogen-activated protein kinase (MAPK) cascade, MAPK KINASE 3 (MKK3)-MAPK 6 (MPK6), which is activated by JA in Arabidopsis...
  13. Mao G, Meng X, Liu Y, Zheng Z, Chen Z, Zhang S. Phosphorylation of a WRKY transcription factor by two pathogen-responsive MAPKs drives phytoalexin biosynthesis in Arabidopsis. Plant Cell. 2011;23:1639-53 pubmed publisher
    ..We recently reported that MPK3 and MPK6, two pathogen-responsive mitogen-activated protein kinases, play essential roles in the induction of camalexin, the ..
  14. Bush S, Krysan P. Mutational evidence that the Arabidopsis MAP kinase MPK6 is involved in anther, inflorescence, and embryo development. J Exp Bot. 2007;58:2181-91 pubmed
    ..approaches were used to investigate the role played by the Arabidopsis mitogen-activated protein (MAP) kinase MPK6 throughout development...
  15. Teige M, Scheikl E, Eulgem T, Doczi R, Ichimura K, Shinozaki K, et al. The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis. Mol Cell. 2004;15:141-52 pubmed
    The Arabidopsis mitogen-activated protein kinase (MAPK) kinase 2 (MKK2) and the downstream MAPKs MPK4 and MPK6 were isolated by functional complementation of osmosensitive yeast mutants...
  16. Nühse T, Peck S, Hirt H, Boller T. Microbial elicitors induce activation and dual phosphorylation of the Arabidopsis thaliana MAPK 6. J Biol Chem. 2000;275:7521-6 pubmed
    ..A. thaliana MAPK 6 (AtMPK6) is the Arabidopsis orthologue of a tobacco MAPK termed salicylate-induced protein kinase, which is activated by ..
  17. Beckers G, Jaskiewicz M, Liu Y, UNDERWOOD W, He S, Zhang S, et al. Mitogen-activated protein kinases 3 and 6 are required for full priming of stress responses in Arabidopsis thaliana. Plant Cell. 2009;21:944-53 pubmed publisher
    ..is associated with accumulation of mRNA and inactive proteins of mitogen-activated protein kinases (MPKs), MPK3 and MPK6. Upon challenge exposure to biotic or abiotic stress, these two enzymes were more strongly activated in primed ..
  18. Zhou C, Cai Z, Guo Y, Gan S. An arabidopsis mitogen-activated protein kinase cascade, MKK9-MPK6, plays a role in leaf senescence. Plant Physiol. 2009;150:167-77 pubmed publisher
    ..Roles of the MAPK signaling module in leaf senescence are unknown. Here, a MAPK cascade involving MKK9-MPK6 is shown to play an important role in regulating leaf senescence in Arabidopsis (Arabidopsis thaliana)...
  19. Kang S, Yang F, Li L, Chen H, Chen S, Zhang J. The Arabidopsis transcription factor BRASSINOSTEROID INSENSITIVE1-ETHYL METHANESULFONATE-SUPPRESSOR1 is a direct substrate of MITOGEN-ACTIVATED PROTEIN KINASE6 and regulates immunity. Plant Physiol. 2015;167:1076-86 pubmed publisher
    ..BES1 interacts with MITOGEN-ACTIVATED PROTEIN KINASE6 (MPK6) and is phosphorylated by MPK6...
  20. Han S, Fang L, Ren X, Wang W, Jiang J. MPK6 controls H2 O2-induced root elongation by mediating Ca2+ influx across the plasma membrane of root cells in Arabidopsis seedlings. New Phytol. 2015;205:695-706 pubmed publisher
    ..Root elongation in seedlings of the loss-of-function mutant Atmpk6 (Arabidopsis thaliana MPK6) was less sensitive to moderate H2 O2 or ABA than that in wild-type (WT) plants...
  21. Ahlfors R, Macioszek V, Rudd J, Brosché M, Schlichting R, Scheel D, et al. Stress hormone-independent activation and nuclear translocation of mitogen-activated protein kinases in Arabidopsis thaliana during ozone exposure. Plant J. 2004;40:512-22 pubmed
    ..These were identified as AtMPK3 and AtMPK6. We also demonstrate that initial AtMPK3 and AtMPK6 activation in response to O3 was not dependent on ET signaling,..
  22. Bartels S, Anderson J, González Besteiro M, Carreri A, Hirt H, Buchala A, et al. MAP kinase phosphatase1 and protein tyrosine phosphatase1 are repressors of salicylic acid synthesis and SNC1-mediated responses in Arabidopsis. Plant Cell. 2009;21:2884-97 pubmed publisher
    ..The dual-specificity phosphatase MAP KINASE PHOSPHATASE1 (MKP1) was previously shown to regulate MAP KINASE6 (MPK6) activation levels and abiotic stress responses in Arabidopsis thaliana...
  23. Lee H, Back K. Mitogen-activated protein kinase pathways are required for melatonin-mediated defense responses in plants. J Pineal Res. 2016;60:327-35 pubmed publisher
    ..Two MAPKs, MPK3 and MPK6, were activated rapidly and transiently by 1 μm melatonin treatment in Arabidopsis...
  24. Liu X, Nguyen X, Kim K, Han H, Yoo J, Lee K, et al. Phosphorylation of the zinc finger transcriptional regulator ZAT6 by MPK6 regulates Arabidopsis seed germination under salt and osmotic stress. Biochem Biophys Res Commun. 2013;430:1054-9 pubmed publisher
    ..Intriguingly, ZAT6 interacts with a stress-responsive mitogen-activated protein kinase MPK6 in vitro and in planta. ZAT6 is phosphorylated by both recombinant and plant endogenous MPK6...
  25. Cheng Z, Li J, Niu Y, Zhang X, Woody O, Xiong Y, et al. Pathogen-secreted proteases activate a novel plant immune pathway. Nature. 2015;521:213-6 pubmed publisher
    ..The ability of P. aeruginosa to infect both plants and animals makes it an excellent model to identify novel immunoregulatory strategies that account for its niche adaptation to diverse host tissues and immune systems. ..
  26. Wang F, Shang Y, Fan B, Yu J, Chen Z. Arabidopsis LIP5, a positive regulator of multivesicular body biogenesis, is a critical target of pathogen-responsive MAPK cascade in plant basal defense. PLoS Pathog. 2014;10:e1004243 pubmed publisher
    ..Arabidopsis LIP5 interacts with MPK6 and MPK3 and is phosphorylated in vitro by activated MPK3 and MPK6 and in vivo upon expression of MPK3/6-activating ..
  27. Wang P, Du Y, Zhao X, Miao Y, Song C. The MPK6-ERF6-ROS-responsive cis-acting Element7/GCC box complex modulates oxidative gene transcription and the oxidative response in Arabidopsis. Plant Physiol. 2013;161:1392-408 pubmed publisher
    ..ERF6 interacted physically with mitogen-activated protein kinase6 (MPK6) and also served as a substrate of MPK6...
  28. Yuasa T, Ichimura K, Mizoguchi T, Shinozaki K. Oxidative stress activates ATMPK6, an Arabidopsis homologue of MAP kinase. Plant Cell Physiol. 2001;42:1012-6 pubmed
    ..We analysed effect of oxidative stress on the activation of ATMPK6, an Arabidopsis thaliana MAPK, in Arabidopsis T87 cultured cells and rosette leaves using anti-ATMPK6 specific ..
  29. Xu D, Chen M, Ma Y, Xu Z, Li L, Chen Y, et al. A G-protein β subunit, AGB1, negatively regulates the ABA response and drought tolerance by down-regulating AtMPK6-related pathway in Arabidopsis. PLoS ONE. 2015;10:e0116385 pubmed publisher
    ..Data showed that AGB1 interacted with protein kinase AtMPK6 that was previously shown to phosphorylate AtVIP1, a transcription factor responding to ABA signaling...
  30. Pecher P, Eschen Lippold L, Herklotz S, Kuhle K, Naumann K, Bethke G, et al. The Arabidopsis thaliana mitogen-activated protein kinases MPK3 and MPK6 target a subclass of 'VQ-motif'-containing proteins to regulate immune responses. New Phytol. 2014;203:592-606 pubmed publisher
    ..A subset of the Arabidopsis thaliana VQ-motif-containing proteins (VQPs) were phosphorylated by the MAPKs MPK3 and MPK6, and renamed MPK3/6-targeted VQPs (MVQs)...
  31. Chang R, Jang C, Branco Price C, Nghiem P, Bailey Serres J. Transient MPK6 activation in response to oxygen deprivation and reoxygenation is mediated by mitochondria and aids seedling survival in Arabidopsis. Plant Mol Biol. 2012;78:109-22 pubmed publisher
    ..Here, we report the rapid and transient activation of MPK3, MPK4 and MPK6 upon oxygen deprivation as well as reoxygenation in seedlings of Arabidopsis thaliana...
  32. Forzani C, Carreri A, de la Fuente van Bentem S, Lecourieux D, Lecourieux F, Hirt H. The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases. FEBS J. 2011;278:1126-36 pubmed publisher
    ..Its activity is required for full activation of the mitogen-activated protein kinases (MAPKs), MPK3 and MPK6, in response to oxidative stress...
  33. Vogel M, Moore M, K nig K, Pecher P, Alsharafa K, Lee J, et al. Fast retrograde signaling in response to high light involves metabolite export, MITOGEN-ACTIVATED PROTEIN KINASE6, and AP2/ERF transcription factors in Arabidopsis. Plant Cell. 2014;26:1151-65 pubmed publisher
    ..Similarly, activation of MITOGEN-ACTIVATED PROTEIN KINASE6 (MPK6) was upregulated after 1 min in the wild type but not in the tpt mutant...
  34. Yue H, Nie S, Xing D. Over-expression of Arabidopsis Bax inhibitor-1 delays methyl jasmonate-induced leaf senescence by suppressing the activation of MAP kinase 6. J Exp Bot. 2012;63:4463-74 pubmed publisher
    ..Further analyses showed that during the process of MeJA-induced senescence, the activity of MPK6, a mitogen-activated protein kinase (MAPK), increased in WT plants, whereas it was significantly suppressed in ..
  35. Miles G, Samuel M, Ranish J, Donohoe S, Sperrazzo G, Ellis B. Quantitative proteomics identifies oxidant-induced, AtMPK6-dependent changes in Arabidopsis thaliana protein profiles. Plant Signal Behav. 2009;4:497-505 pubmed
    ..thaliana, oxidant-induced signalling has been shown to utilize the mitogen-activated protein kinase (MAPK), AtMPK6. To identify proteins whose accumulation is altered by ozone in an AtMPK6-dependent manner we employed isotope-..
  36. Ouaked F, Rozhon W, Lecourieux D, Hirt H. A MAPK pathway mediates ethylene signaling in plants. EMBO J. 2003;22:1282-8 pubmed
    ..ACC) in Medicago and Arabidopsis: In Medicago, the ACC-activated MAPKs were SIMK and MMK3, while in Arabidopsis MPK6 and another MAPK were identified. Medicago SIMKK specifically mediated ACC-induced activation of SIMK and MMK3...
  37. . Mitogen-activated protein kinase cascades in plants: a new nomenclature. Trends Plant Sci. 2002;7:301-8 pubmed
    ..Here, we propose a simplified nomenclature for Arabidopsis MAPKs and MAPK kinases that might also serve as a basis for standard annotation of these gene families in all plants. ..
  38. Zhang Y, Guo X, Dong J. Phosphorylation of the Polarity Protein BASL Differentiates Asymmetric Cell Fate through MAPKs and SPCH. Curr Biol. 2016;26:2957-2965 pubmed publisher
    ..a canonical mitogen-activated protein kinase (MAPK) pathway that recruits the MAPKK kinase YODA (YDA) and MAPK 6 (MPK6) to the cortical polarity site [4]...
  39. Kim J, Woo D, Kim S, Lee S, Park H, Seok H, et al. Arabidopsis MKKK20 is involved in osmotic stress response via regulation of MPK6 activity. Plant Cell Rep. 2012;31:217-24 pubmed publisher
    ..In-gel kinase assay showed that MKKK20 regulates the activity of MPK6 under NaCl, cold, and H(2)O(2) treatments...
  40. Droillard M, Boudsocq M, Barbier Brygoo H, Laurière C. Different protein kinase families are activated by osmotic stresses in Arabidopsis thaliana cell suspensions. Involvement of the MAP kinases AtMPK3 and AtMPK6. FEBS Lett. 2002;527:43-50 pubmed
    ..Using specific antibodies, two of the MAP kinases were identified as AtMPK6 and AtMPK3...
  41. Sethi V, Raghuram B, Sinha A, Chattopadhyay S. A mitogen-activated protein kinase cascade module, MKK3-MPK6 and MYC2, is involved in blue light-mediated seedling development in Arabidopsis. Plant Cell. 2014;26:3343-57 pubmed publisher
    ..Here, we show that MKK3-MPK6 is activated by blue light in a MYC2-dependent manner...
  42. Pérez Salamó I, Papdi C, Rigó G, Zsigmond L, Vilela B, Lumbreras V, et al. The heat shock factor A4A confers salt tolerance and is regulated by oxidative stress and the mitogen-activated protein kinases MPK3 and MPK6. Plant Physiol. 2014;165:319-34 pubmed publisher
    ..HSFA4A interacts with mitogen-activated protein kinases MPK3 and MPK6 in yeast and plant cells...
  43. López Bucio J, Raya González J, Ravelo Ortega G, Ruiz Herrera L, Ramos Vega M, León P, et al. Mitogen activated protein kinase 6 and MAP kinase phosphatase 1 are involved in the response of Arabidopsis roots to L-glutamate. Plant Mol Biol. 2018;96:339-351 pubmed publisher
    ..genes, the critical roles of L-Glu in primary root growth acting through the mitogen-activated protein kinase 6 (MPK6) and the dual specificity serine-threonine-tyrosine phosphatase MKP1 could be revealed...
  44. Doczi R, Brader G, Pettkó Szandtner A, Rajh I, Djamei A, Pitzschke A, et al. The Arabidopsis mitogen-activated protein kinase kinase MKK3 is upstream of group C mitogen-activated protein kinases and participates in pathogen signaling. Plant Cell. 2007;19:3266-79 pubmed
    ..Flagellin-derived flg22 peptide strongly activated MPK6 but resulted in poor activation of MPK7...
  45. Xu J, Li Y, Wang Y, Liu H, Lei L, Yang H, et al. Activation of MAPK kinase 9 induces ethylene and camalexin biosynthesis and enhances sensitivity to salt stress in Arabidopsis. J Biol Chem. 2008;283:26996-7006 pubmed publisher
    ..We demonstrate that MKK9, an MKK, is an upstream activator of the MPKs MPK3 and MPK6 both in vitro and in planta...
  46. Jia W, Li B, Li S, Liang Y, Wu X, Ma M, et al. Mitogen-Activated Protein Kinase Cascade MKK7-MPK6 Plays Important Roles in Plant Development and Regulates Shoot Branching by Phosphorylating PIN1 in Arabidopsis. PLoS Biol. 2016;14:e1002550 pubmed publisher
    ..In this study, we found that MPK3 and MPK6 are the substrates for phosphorylation by MKK7 in planta...
  47. Anderson J, Bartels S, González Besteiro M, Shahollari B, Ulm R, Peck S. Arabidopsis MAP Kinase Phosphatase 1 (AtMKP1) negatively regulates MPK6-mediated PAMP responses and resistance against bacteria. Plant J. 2011;67:258-68 pubmed publisher
    ..In Arabidopsis, several kinases, including the mitogen-activated protein kinases (MAPKs) MPK6 and MPK3, are rapidly activated after PAMP treatment, and are thought to positively regulate a wide array of ..
  48. Li H, Ding Y, Shi Y, Zhang X, Zhang S, Gong Z, et al. MPK3- and MPK6-Mediated ICE1 Phosphorylation Negatively Regulates ICE1 Stability and Freezing Tolerance in Arabidopsis. Dev Cell. 2017;43:630-642.e4 pubmed publisher
    ..Here, we report that mitogen-activated protein kinase 3 (MPK3) and MPK6 interact with and phosphorylate ICE1, which reduces its stability and transcriptional activity...
  49. Park H, Song E, Nguyen X, Lee K, Kim K, Kim H, et al. Arabidopsis MAP kinase phosphatase 1 is phosphorylated and activated by its substrate AtMPK6. Plant Cell Rep. 2011;30:1523-31 pubmed publisher
    ..Here, we examined the phosphorylation of AtMKP1 by its substrate AtMPK6. Intriguingly, AtMKP1 was phosphorylated by AtMPK6, one of AtMKP1 substrates...
  50. Jewaria P, Hara T, Tanaka H, Kondo T, Betsuyaku S, Sawa S, et al. Differential effects of the peptides Stomagen, EPF1 and EPF2 on activation of MAP kinase MPK6 and the SPCH protein level. Plant Cell Physiol. 2013;54:1253-62 pubmed publisher
    ..We show that EPF1 and EPF2 are able to activate the MAP kinase MPK6, and that both EPF1 and EPF2 are able to decrease the SPCH level, whereas stomagen is able to increase it...
  51. Sidonskaya E, Schweighofer A, Shubchynskyy V, Kammerhofer N, Hofmann J, Wieczorek K, et al. Plant resistance against the parasitic nematode Heterodera schachtii is mediated by MPK3 and MPK6 kinases, which are controlled by the MAPK phosphatase AP2C1 in Arabidopsis. J Exp Bot. 2016;67:107-18 pubmed publisher
    ..MAPK phosphatases control kinase activities and the signalling outcome. The involvement and the role of MPK3 and MPK6, as well as the MAPK phosphatase AP2C1, is demonstrated during parasitism of the beet cyst nematode Heterodera ..
  52. Zhao F, Zheng Y, Zeng T, Sun R, Yang J, Li Y, et al. Phosphorylation of SPOROCYTELESS/NOZZLE by the MPK3/6 Kinase Is Required for Anther Development. Plant Physiol. 2017;173:2265-2277 pubmed publisher
    ..Here, we report that the protein kinases MPK3 and MPK6 can both interact with SPL in vitro and in vivo and can phosphorylate the SPL protein in vitro...
  53. Zeng Q, Chen J, Ellis B. AtMPK4 is required for male-specific meiotic cytokinesis in Arabidopsis. Plant J. 2011;67:895-906 pubmed publisher
  54. Lei L, Li Y, Wang Q, Xu J, Chen Y, Yang H, et al. Activation of MKK9-MPK3/MPK6 enhances phosphate acquisition in Arabidopsis thaliana. New Phytol. 2014;203:1146-60 pubmed publisher
    ..Using in-gel kinase assays, we determined the activities of MPK3 and MPK6 in Arabidopsis thaliana seedlings under both LP and Pi-sufficient (Murashige and Skoog, MS) conditions...
  55. Okawa C, Ishikawa A. MPK6 contributes to non-host resistance to magnaporthe oryzae in Arabidopsis thaliana. Biosci Biotechnol Biochem. 2013;77:1320-2 pubmed
    The rate of entry of Magnaporthe oryzae into pen2 mpk6 plants was higher than that into pen2 plants. The infection hyphae in the pen2 mpk6 plants were longer than those in the pen2 plants...
  56. Lee J, Huh K, Bhargava A, Ellis B. Comprehensive analysis of protein-protein interactions between Arabidopsis MAPKs and MAPK kinases helps define potential MAPK signalling modules. Plant Signal Behav. 2008;3:1037-41 pubmed
    ..The resulting data confirm a number of earlier reported MKK-MPK relationships, but also reveal a more extensive pattern of interactions that should help to guide future analyses of MAPK signalling in plants. ..
  57. Kovtun Y, Chiu W, Tena G, Sheen J. Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants. Proc Natl Acad Sci U S A. 2000;97:2940-5 pubmed
    ..kinase kinase kinase, ANP1, which initiates a phosphorylation cascade involving two stress MAPKs, AtMPK3 and AtMPK6. Constitutively active ANP1 mimics the H(2)O(2) effect and initiates the MAPK cascade that induces specific stress-..
  58. Guan Y, Meng X, Khanna R, LaMontagne E, Liu Y, Zhang S. Phosphorylation of a WRKY transcription factor by MAPKs is required for pollen development and function in Arabidopsis. PLoS Genet. 2014;10:e1004384 pubmed publisher
    ..using LAT52, a strong pollen-specific promoter, epitope-tagged WRKY34 is temporally phosphorylated by MPK3 and MPK6, two mitogen-activated protein kinases (MAPKs, or MPKs), at early stages in pollen development...
  59. Ulm R, Ichimura K, Mizoguchi T, Peck S, Zhu T, Wang X, et al. Distinct regulation of salinity and genotoxic stress responses by Arabidopsis MAP kinase phosphatase 1. EMBO J. 2002;21:6483-93 pubmed
    ..Here, we identify a set of MAPKs that interact with MKP1, and show that the activity level of one of these, MPK6, is regulated by MKP1 in vivo...
  60. Nie S, Xu H. Riboflavin-Induced Disease Resistance Requires the Mitogen-Activated Protein Kinases 3 and 6 in Arabidopsis thaliana. PLoS ONE. 2016;11:e0153175 pubmed publisher
    ..Further was suggested that the transcript levels of mitogen-activated protein kinases, MPK3 and MPK6, were primed under riboflavin. Upon infection by Pst DC3000, these two enzymes were more strongly activated...
  61. Kannan P, Pandey D, Gupta A, Punetha H, Taj G, Kumar A. Expression analysis of MAP2K9 and MAPK6 during pathogenesis of Alternaria blight in Arabidopsis thaliana ecotype Columbia. Mol Biol Rep. 2012;39:4439-44 pubmed publisher
    ..quantitative real time PCR based approaches were used to determine the change in transcript profile of MAP2K9 and MAPK6 in leaves of A. thaliana ecotpe Columbia at early, middle and late stages of Alternaria blight infection...
  62. L pez Bucio J, Dubrovsky J, Raya Gonz lez J, Ugartechea Chirino Y, L pez Bucio J, de Luna Valdez L, et al. Arabidopsis thaliana mitogen-activated protein kinase 6 is involved in seed formation and modulation of primary and lateral root development. J Exp Bot. 2014;65:169-83 pubmed publisher
    ..In this study, we characterized the role of MAPK 6 (MPK6) in Arabidopsis embryo development and in post-embryonic root system architecture...
  63. Hoang M, Nguyen X, Lee K, Kwon Y, Pham H, Park H, et al. Phosphorylation by AtMPK6 is required for the biological function of AtMYB41 in Arabidopsis. Biochem Biophys Res Commun. 2012;422:181-6 pubmed publisher
    ..Here, we provide evidence that MYB41 represents a new target of MPK6. MYB41 interacts with MPK6 not only in vitro but also in planta...
  64. Li K, Yang F, Miao Y, Song C. Abscisic acid signaling is involved in regulating the mitogen-activated protein kinase cascade module, AIK1-MKK5-MPK6. Plant Signal Behav. 2017;12:e1321188 pubmed publisher
    ..The activity of ABA-activated MPK6 was decreased in abi1 abi2, and abi1 abi2 hab1, and increased in snrk2.2 snrk2.3 and pyr1 pyl1 pyl2 pyl4 mutants...
  65. Schweighofer A, Shubchynskyy V, Kazanaviciute V, Djamei A, Meskiene I. Bimolecular fluorescent complementation (BiFC) by MAP kinases and MAPK phosphatases. Methods Mol Biol. 2014;1171:147-58 pubmed publisher
    ..The interactions of the MAPKs MPK3, MKP4 and MPK6 with the phosphatases AP2C1 and AP2C3 have been tested.
  66. Wang B, Qin X, Wu J, Deng H, Li Y, Yang H, et al. Analysis of crystal structure of Arabidopsis MPK6 and generation of its mutants with higher activity. Sci Rep. 2016;6:25646 pubmed publisher
    ..Here, we report the crystal structure of Arabidopsis MPK6 at a 3.0 Å resolution...
  67. Nguyen X, Kim S, Lee K, Kim K, Liu X, Han H, et al. Identification of a C2H2-type zinc finger transcription factor (ZAT10) from Arabidopsis as a substrate of MAP kinase. Plant Cell Rep. 2012;31:737-45 pubmed publisher
    ..and in vivo protein-protein interaction analyses, we demonstrated that ZAT10 directly interacted with MPK3 and MPK6. ZAT10 was phosphorylated by recombinant Arabidopsis MPK3 and MPK6 in a kinase assay...
  68. Xu J, Xie J, Yan C, Zou X, Ren D, Zhang S. A chemical genetic approach demonstrates that MPK3/MPK6 activation and NADPH oxidase-mediated oxidative burst are two independent signaling events in plant immunity. Plant J. 2014;77:222-34 pubmed publisher
    ..Arabidopsis has at least four PAMP/pathogen-responsive MAPKs: MPK3, MPK6, MPK4 and MPK11...
  69. Schikora A, Schenk S, Stein E, Molitor A, Zuccaro A, Kogel K. N-acyl-homoserine lactone confers resistance toward biotrophic and hemibiotrophic pathogens via altered activation of AtMPK6. Plant Physiol. 2011;157:1407-18 pubmed publisher
    ..Oxo-C14-HSL promoted a stronger activation of mitogen-activated protein kinases AtMPK3 and AtMPK6 when challenged with flg22, followed by a higher expression of the defense-related transcription factors WRKY22 ..
  70. Zhang Y, Liu J, Chai J, Xing D. Mitogen-activated protein kinase 6 mediates nuclear translocation of ORE3 to promote ORE9 gene expression in methyl jasmonate-induced leaf senescence. J Exp Bot. 2016;67:83-94 pubmed publisher
    ..Further analyses showed that mitogen-activated protein kinase 6 (MPK6) promoted CEND cleavage and nuclear translocation...