Gene Symbol: MPK4
Description: MAP kinase 4
Alias: ATMPK4, F2N1.1, F2N1_1, MAP kinase 4, MAPK4, MAP kinase 4
Species: thale cress
Products:     MPK4

Top Publications

  1. Qiu J, Fiil B, Petersen K, Nielsen H, Botanga C, Thorgrimsen S, et al. Arabidopsis MAP kinase 4 regulates gene expression through transcription factor release in the nucleus. EMBO J. 2008;27:2214-21 pubmed publisher
    ..We report that, in the absence of pathogens, Arabidopsis MAP kinase 4 (MPK4) exists in nuclear complexes with the WRKY33 transcription factor...
  2. Droillard M, Boudsocq M, Barbier Brygoo H, Laurière C. Involvement of MPK4 in osmotic stress response pathways in cell suspensions and plantlets of Arabidopsis thaliana: activation by hypoosmolarity and negative role in hyperosmolarity tolerance. FEBS Lett. 2004;574:42-8 pubmed
    ..The third MAP kinase was identified here to MPK4, using a corresponding specific antibody...
  3. Meszaros T, Helfer A, Hatzimasoura E, Magyar Z, Serazetdinova L, Rios G, et al. The Arabidopsis MAP kinase kinase MKK1 participates in defence responses to the bacterial elicitor flagellin. Plant J. 2006;48:485-98 pubmed
    ..The endogenous Arabidopsis MAP kinase kinase MKK1 is activated in cells treated with flg22, phosphorylates the MAPK MPK4 in vitro, and activates it in vivo in protoplasts...
  4. Suarez Rodriguez M, Adams Phillips L, Liu Y, Wang H, Su S, Jester P, et al. MEKK1 is required for flg22-induced MPK4 activation in Arabidopsis plants. Plant Physiol. 2007;143:661-9 pubmed
    ..analysis of plants carrying T-DNA knockout alleles indicated that MEKK1 is required for flg22-induced activation of MPK4 but not MPK3 or MPK6...
  5. Lee J, Huh K, Bhargava A, Ellis B. Comprehensive analysis of protein-protein interactions between Arabidopsis MAPKs and MAPK kinases helps define potential MAPK signalling modules. Plant Signal Behav. 2008;3:1037-41 pubmed
    ..The resulting data confirm a number of earlier reported MKK-MPK relationships, but also reveal a more extensive pattern of interactions that should help to guide future analyses of MAPK signalling in plants. ..
  6. . Mitogen-activated protein kinase cascades in plants: a new nomenclature. Trends Plant Sci. 2002;7:301-8 pubmed
    ..Here, we propose a simplified nomenclature for Arabidopsis MAPKs and MAPK kinases that might also serve as a basis for standard annotation of these gene families in all plants. ..
  7. Andreasson E, Jenkins T, Brodersen P, Thorgrimsen S, Petersen N, Zhu S, et al. The MAP kinase substrate MKS1 is a regulator of plant defense responses. EMBO J. 2005;24:2579-89 pubmed
    Arabidopsis MAP kinase 4 (MPK4) functions as a regulator of pathogen defense responses, because it is required for both repression of salicylic acid (SA)-dependent resistance and for activation of jasmonate (JA)-dependent defense gene ..
  8. Brodersen P, Petersen M, Bjørn Nielsen H, Zhu S, Newman M, Shokat K, et al. Arabidopsis MAP kinase 4 regulates salicylic acid- and jasmonic acid/ethylene-dependent responses via EDS1 and PAD4. Plant J. 2006;47:532-46 pubmed
    Arabidopsis MPK4 has been implicated in plant defense regulation because mpk4 knockout plants exhibit constitutive activation of salicylic acid (SA)-dependent defenses, but fail to induce jasmonic acid (JA) defense marker genes in ..
  9. Ichimura K, Casais C, Peck S, Shinozaki K, Shirasu K. MEKK1 is required for MPK4 activation and regulates tissue-specific and temperature-dependent cell death in Arabidopsis. J Biol Chem. 2006;281:36969-76 pubmed
    ..Instead, MEKK1 is essential for activation of MPK4, a MAPK that negatively regulates systemic acquired resistance...

More Information


  1. Teige M, Scheikl E, Eulgem T, Doczi R, Ichimura K, Shinozaki K, et al. The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis. Mol Cell. 2004;15:141-52 pubmed
    The Arabidopsis mitogen-activated protein kinase (MAPK) kinase 2 (MKK2) and the downstream MAPKs MPK4 and MPK6 were isolated by functional complementation of osmosensitive yeast mutants...
  2. Berriri S, Garcia A, Frei dit Frey N, Rozhon W, Pateyron S, Leonhardt N, et al. Constitutively active mitogen-activated protein kinase versions reveal functions of Arabidopsis MPK4 in pathogen defense signaling. Plant Cell. 2012;24:4281-93 pubmed publisher
    ..As a proof-of-concept, Arabidopsis MAPK4 (MPK4) function in plant immunity was investigated...
  3. Walia A, Lee J, Wasteneys G, Ellis B. Arabidopsis mitogen-activated protein kinase MPK18 mediates cortical microtubule functions in plant cells. Plant J. 2009;59:565-75 pubmed publisher
    ..We propose a model whereby the PHS1-MPK18 signalling module is involved in a phosphorylation/dephosphorylation switch that regulates cortical microtubule functions. ..
  4. Mizoguchi T, Hayashida N, Yamaguchi Shinozaki K, Kamada H, Shinozaki K. ATMPKs: a gene family of plant MAP kinases in Arabidopsis thaliana. FEBS Lett. 1993;336:440-4 pubmed
    ..Genes for MAP kinases in Arabidopsis constitute a family that contains more than seven members. Sequence analysis suggests that there are at least three subfamilies in the family of Arabidopsis genes for MAP kinases. ..
  5. Su S, Suarez Rodriguez M, Krysan P. Genetic interaction and phenotypic analysis of the Arabidopsis MAP kinase pathway mutations mekk1 and mpk4 suggests signaling pathway complexity. FEBS Lett. 2007;581:3171-7 pubmed
    It has been shown that the Arabidopsis MEK kinase MEKK1 acts upstream of the MAP kinase MPK4 to negatively regulate salicylic acid-dependent defense-response pathways...
  6. Kosetsu K, Matsunaga S, Nakagami H, Colcombet J, Sasabe M, Soyano T, et al. The MAP kinase MPK4 is required for cytokinesis in Arabidopsis thaliana. Plant Cell. 2010;22:3778-90 pubmed publisher
    ..We show here that a MAP kinase, MPK4, is required for the formation of the cell plate in Arabidopsis thaliana...
  7. Mizoguchi T, Ichimura K, Irie K, Morris P, Giraudat J, Matsumoto K, et al. Identification of a possible MAP kinase cascade in Arabidopsis thaliana based on pairwise yeast two-hybrid analysis and functional complementation tests of yeast mutants. FEBS Lett. 1998;437:56-60 pubmed
    ..Specific protein-protein interactions between ATMPK4 (a MAPK) and MEK1 (a MAPKK) and interactions between MEK1 and ATMEKK1 (a MAPKKK) were detected by using the 2-..
  8. Beck M, Komis G, Muller J, Menzel D, Samaj J. Arabidopsis homologs of nucleus- and phragmoplast-localized kinase 2 and 3 and mitogen-activated protein kinase 4 are essential for microtubule organization. Plant Cell. 2010;22:755-71 pubmed publisher
    ..kinase 2 (ANP2) and 3 (ANP3) genes and a homozygous recessive mutant in the mitogen-activated protein kinase 4 (MPK4) gene of Arabidopsis exhibit deficiencies in the overall microtubule (MT) organization, which result in abnormal ..
  9. Furuya T, Matsuoka D, Nanmori T. Membrane rigidification functions upstream of the MEKK1-MKK2-MPK4 cascade during cold acclimation in Arabidopsis thaliana. FEBS Lett. 2014;588:2025-30 pubmed publisher
    The MEKK1-MKK2-MPK4 cascade is activated during cold acclimation. However, little is known regarding the perception of low temperature...
  10. Gao M, Wang X, Wang D, Xu F, Ding X, Zhang Z, et al. Regulation of cell death and innate immunity by two receptor-like kinases in Arabidopsis. Cell Host Microbe. 2009;6:34-44 pubmed publisher
    ..leads to extensive cell death, activation of constitutive defense responses, and impairment in the activation of MPK4, a negative regulator of plant resistance (R) protein signaling, by flagellin...
  11. Schweighofer A, Kazanaviciute V, Scheikl E, Teige M, Doczi R, Hirt H, et al. The PP2C-type phosphatase AP2C1, which negatively regulates MPK4 and MPK6, modulates innate immunity, jasmonic acid, and ethylene levels in Arabidopsis. Plant Cell. 2007;19:2213-24 pubmed
    ..Ser/Thr phosphatase of type 2C, is a novel stress signal regulator that inactivates the stress-responsive MAPKs MPK4 and MPK6...
  12. Cui H, Wang Y, Xue L, Chu J, Yan C, Fu J, et al. Pseudomonas syringae effector protein AvrB perturbs Arabidopsis hormone signaling by activating MAP kinase 4. Cell Host Microbe. 2010;7:164-75 pubmed publisher
    ..Here we show that these effects of AvrB require the Arabidopsis mitogen-activated protein kinase MAP kinase 4 (MPK4), HSP90 chaperone components, and the AvrB-interacting protein, RIN4...
  13. Li B, Jiang S, Yu X, Cheng C, Chen S, Cheng Y, et al. Phosphorylation of trihelix transcriptional repressor ASR3 by MAP KINASE4 negatively regulates Arabidopsis immunity. Plant Cell. 2015;27:839-56 pubmed publisher
    ..MAMP treatments induce rapid phosphorylation of ASR3 at threonine 189 via MPK4, a mitogen-activated protein kinase that negatively regulates PTI responses downstream of multiple MAMP receptors...
  14. Brock A, Willmann R, Kolb D, Grefen L, Lajunen H, Bethke G, et al. The Arabidopsis mitogen-activated protein kinase phosphatase PP2C5 affects seed germination, stomatal aperture, and abscisic acid-inducible gene expression. Plant Physiol. 2010;153:1098-111 pubmed publisher
    ..The PP2C5 protein colocalizes and directly interacts with stress-induced MPK3, MPK4, and MPK6, predominantly in the nucleus. Importantly, altered PP2C5 levels affect MAPK activation...
  15. Beck M, Komis G, Ziemann A, Menzel D, Samaj J. Mitogen-activated protein kinase 4 is involved in the regulation of mitotic and cytokinetic microtubule transitions in Arabidopsis thaliana. New Phytol. 2011;189:1069-83 pubmed publisher
    ..nucleus and phragmoplast associated kinase (anp) anp2anp3, and the mitogen-activated protein kinase (MAPK) 4 mutant mpk4 of Arabidopsis thaliana show prominent cytokinetic defects...
  16. Eschen Lippold L, Jiang X, Elmore J, Mackey D, Shan L, Coaker G, et al. Bacterial AvrRpt2-Like Cysteine Proteases Block Activation of the Arabidopsis Mitogen-Activated Protein Kinases, MPK4 and MPK11. Plant Physiol. 2016;171:2223-38 pubmed publisher
    ..that AvrRpt2 specifically suppresses the flagellin-induced phosphorylation of Arabidopsis (Arabidopsis thaliana) MPK4 and MPK11 but not MPK3 or MPK6...
  17. Schweighofer A, Shubchynskyy V, Kazanaviciute V, Djamei A, Meskiene I. Bimolecular fluorescent complementation (BiFC) by MAP kinases and MAPK phosphatases. Methods Mol Biol. 2014;1171:147-58 pubmed publisher
    ..The interactions of the MAPKs MPK3, MKP4 and MPK6 with the phosphatases AP2C1 and AP2C3 have been tested. ..
  18. Petersen K, Qiu J, Lütje J, Fiil B, Hansen S, Mundy J, et al. Arabidopsis MKS1 is involved in basal immunity and requires an intact N-terminal domain for proper function. PLoS ONE. 2010;5:e14364 pubmed publisher
    ..b>MAP kinase 4 (MPK4) functions downstream of innate immune receptors via a nuclear substrate MKS1 to regulate the activity of ..
  19. Chang R, Jang C, Branco Price C, Nghiem P, Bailey Serres J. Transient MPK6 activation in response to oxygen deprivation and reoxygenation is mediated by mitochondria and aids seedling survival in Arabidopsis. Plant Mol Biol. 2012;78:109-22 pubmed publisher
    ..Here, we report the rapid and transient activation of MPK3, MPK4 and MPK6 upon oxygen deprivation as well as reoxygenation in seedlings of Arabidopsis thaliana...
  20. Sörensson C, Lenman M, Veide Vilg J, Schopper S, Ljungdahl T, Grøtli M, et al. Determination of primary sequence specificity of Arabidopsis MAPKs MPK3 and MPK6 leads to identification of new substrates. Biochem J. 2012;446:271-8 pubmed publisher
    ..MPK3 and MPK6 phosphorylated all proteins tested more efficiently than did another MAPK, MPK4. These results indicate that the amino acid residues in the primary sequence surrounding the phosphorylation site ..
  21. Xing Y, Jia W, Zhang J. AtMKK1 mediates ABA-induced CAT1 expression and H2O2 production via AtMPK6-coupled signaling in Arabidopsis. Plant J. 2008;54:440-51 pubmed publisher
    ..The data suggest AtMKK1-AtMPK6 to be a key module in an ABA-dependent signaling cascade causing H(2)O(2) production and stress responses. ..
  22. Gawronski P, Witoń D, Vashutina K, Bederska M, Betliński B, Rusaczonek A, et al. Mitogen-activated protein kinase 4 is a salicylic acid-independent regulator of growth but not of photosynthesis in Arabidopsis. Mol Plant. 2014;7:1151-66 pubmed publisher
    ..One of the best-characterized MAPKs in Arabidopsis thaliana is MPK4, which was shown to be a negative regulator of systemic-acquired resistance...
  23. Kong Q, Qu N, Gao M, Zhang Z, Ding X, Yang F, et al. The MEKK1-MKK1/MKK2-MPK4 kinase cascade negatively regulates immunity mediated by a mitogen-activated protein kinase kinase kinase in Arabidopsis. Plant Cell. 2012;24:2225-36 pubmed publisher
    In Arabidopsis thaliana, the MEKK1-MKK1/MKK2-MPK4 mitogen-activated protein (MAP) kinase cascade represses cell death and immune responses...
  24. Zeng Q, Chen J, Ellis B. AtMPK4 is required for male-specific meiotic cytokinesis in Arabidopsis. Plant J. 2011;67:895-906 pubmed publisher
    ..anq1/mkk6 mutants, and protein-protein interaction analysis defines a putative signalling module linking AtNACK2/TES/STUD, AtANP3, AtMKK6 and AtMPK4 together as a cascade that facilitates male-specific meiotic cytokinesis in Arabidopsis.
  25. Su S, Bush S, Zaman N, Stecker K, Sussman M, Krysan P. Deletion of a tandem gene family in Arabidopsis: increased MEKK2 abundance triggers autoimmunity when the MEKK1-MKK1/2-MPK4 signaling cascade is disrupted. Plant Cell. 2013;25:1895-910 pubmed publisher
    An Arabidopsis thaliana mitogen-activated protein (MAP) kinase cascade composed of MEKK1, MKK1/MKK2, and MPK4 was previously described as a negative regulator of defense response...
  26. Roux M, Rasmussen M, Palma K, Lolle S, Regué Ã, Bethke G, et al. The mRNA decay factor PAT1 functions in a pathway including MAP kinase 4 and immune receptor SUMM2. EMBO J. 2015;34:593-608 pubmed publisher
    ..For example, Arabidopsis MAP kinase 4 (MPK4) regulates the expression of a subset of defense genes via at least one WRKY transcription factor...
  27. Ichimura K, Mizoguchi T, Yoshida R, Yuasa T, Shinozaki K. Various abiotic stresses rapidly activate Arabidopsis MAP kinases ATMPK4 and ATMPK6. Plant J. 2000;24:655-65 pubmed
    ..hyper-osmolarity, touch and wounding induce rapid and transient activation of the Arabidopsis MAP kinases ATMPK4 and ATMPK6...
  28. Ichimura K, Mizoguchi T, Irie K, Morris P, Giraudat J, Matsumoto K, et al. Isolation of ATMEKK1 (a MAP kinase kinase kinase)-interacting proteins and analysis of a MAP kinase cascade in Arabidopsis. Biochem Biophys Res Commun. 1998;253:532-43 pubmed
    ..In two-hybrid screening using ATMEKK1 as bait, we isolated a novel MAPKK homologue, ATMKK2, a MAPK homologue, ATMPK4, and an unknown protein. ATMKK2 has high sequence similarity with MEK1 (a MAPKK) in Arabidopsis...
  29. Innes R. Mapping out the roles of MAP kinases in plant defense. Trends Plant Sci. 2001;6:392-4 pubmed
    ..Recent papers from two groups have shed new light on the function of two different MAP kinases. The Arabidopsis MPK4 gene appears to negatively regulate salicylic acid-mediated defense responses and positively regulate jasmonic acid-..
  30. Takahashi Y, Soyano T, Kosetsu K, Sasabe M, Machida Y. HINKEL kinesin, ANP MAPKKKs and MKK6/ANQ MAPKK, which phosphorylates and activates MPK4 MAPK, constitute a pathway that is required for cytokinesis in Arabidopsis thaliana. Plant Cell Physiol. 2010;51:1766-76 pubmed publisher
    ..MKK6/ANQ phosphorylated MPK4 MAPK in vitro to activate the latter kinase. Thus cytokinesis in A...
  31. Ulm R, Ichimura K, Mizoguchi T, Peck S, Zhu T, Wang X, et al. Distinct regulation of salinity and genotoxic stress responses by Arabidopsis MAP kinase phosphatase 1. EMBO J. 2002;21:6483-93 pubmed
    ..Indeed, mkp1 plants have increased resistance to salinity. Thus MKP1 apparently plays a pivotal role in the integration and fine-tuning of plant responses to various environmental challenges. ..
  32. Gao M, Liu J, Bi D, Zhang Z, Cheng F, Chen S, et al. MEKK1, MKK1/MKK2 and MPK4 function together in a mitogen-activated protein kinase cascade to regulate innate immunity in plants. Cell Res. 2008;18:1190-8 pubmed publisher
    ..In a genetic screen to search for mutants with constitutive defense responses, we identified multiple alleles of mpk4 and mekk1 that exhibit cell death and constitutive defense responses...
  33. Li S, Wang W, Gao J, Yin K, Wang R, Wang C, et al. MYB75 Phosphorylation by MPK4 Is Required for Light-Induced Anthocyanin Accumulation in Arabidopsis. Plant Cell. 2016;28:2866-2883 pubmed
    ..Here, we report that MYB75 interacts with and is phosphorylated by MAP KINASE4 (MPK4). Their interaction is dependent on MPK4 kinase activity and is required for full function of MYB75...