MAP65-1

Summary

Gene Symbol: MAP65-1
Description: microtubule-associated proteins 65-1
Alias: ATMAP65-1, MCO15.18, MCO15_18, microtubule-associated proteins 65-1, microtubule-associated proteins 65-1
Species: thale cress

Top Publications

  1. Smertenko A, Chang H, Wagner V, Kaloriti D, Fenyk S, Sonobe S, et al. The Arabidopsis microtubule-associated protein AtMAP65-1: molecular analysis of its microtubule bundling activity. Plant Cell. 2004;16:2035-47 pubmed
    ..Moreover, using an antiserum raised to AtMAP65-1, we show that AtMAP65-1 binds microtubules at specific stages of the cell cycle. ..
  2. Li H, Mao T, Zhang Z, Yuan M. The AtMAP65-1 cross-bridge between microtubules is formed by one dimer. Plant Cell Physiol. 2007;48:866-74 pubmed
  3. Lucas J, Courtney S, Hassfurder M, Dhingra S, Bryant A, Shaw S. Microtubule-associated proteins MAP65-1 and MAP65-2 positively regulate axial cell growth in etiolated Arabidopsis hypocotyls. Plant Cell. 2011;23:1889-903 pubmed publisher
    ..We conclude that MAP65-1 and MAP65-2 play a critical role in the microtubule-dependent mechanism for specifying axial cell growth in the expanding hypocotyl, independent of any mechanical role in microtubule array organization. ..
  4. Tulin A, McClerklin S, Huang Y, Dixit R. Single-molecule analysis of the microtubule cross-linking protein MAP65-1 reveals a molecular mechanism for contact-angle-dependent microtubule bundling. Biophys J. 2012;102:802-9 pubmed publisher
    ..Together, our data show that the rod domain of MAP65-1 acts both as a spacer and as a structural element that specifies the MT encounter angles that are conducive for bundling. ..
  5. Gaillard J, Neumann E, Van Damme D, Stoppin Mellet V, Ebel C, Barbier E, et al. Two microtubule-associated proteins of Arabidopsis MAP65s promote antiparallel microtubule bundling. Mol Biol Cell. 2008;19:4534-44 pubmed publisher
  6. Van Damme D, Van Poucke K, Boutant E, Ritzenthaler C, Inze D, Geelen D. In vivo dynamics and differential microtubule-binding activities of MAP65 proteins. Plant Physiol. 2004;136:3956-67 pubmed
    ..The expression of AtMAP65-4 peaked at mitosis, in agreement with a function related to spindle formation, whereas AtMAP65-1 and AtMAP65-5 were expressed throughout the cell cycle. ..
  7. Lam B, Sage T, Bianchi F, Blumwald E. Role of SH3 domain-containing proteins in clathrin-mediated vesicle trafficking in Arabidopsis. Plant Cell. 2001;13:2499-512 pubmed
    ..Hence, AtSH3P1 may perform regulatory and/or scaffolding roles during the transition of fission and the uncoating of clathrin-coated vesicles. ..
  8. Zhang Q, Lin F, Mao T, Nie J, Yan M, Yuan M, et al. Phosphatidic acid regulates microtubule organization by interacting with MAP65-1 in response to salt stress in Arabidopsis. Plant Cell. 2012;24:4555-76 pubmed publisher
    ..The identification of MAP65-1 as a target of PA reveals a functional connection between membrane lipids and the cytoskeleton in environmental stress signaling. ..
  9. Yu R, Sun L, Li X, Wang X, Yuan M, Wu Z. [Localization of microtubule-associated protein AtMAP65-1 in guard cells of arabidopsis]. Fen Zi Xi Bao Sheng Wu Xue Bao. 2007;40:258-62 pubmed
    ..The results suggest that AtMAP65-1 binds cortical microtubules, and it may regulate the arrangement of cortical microtubules, which helps us gain more insights into the role of AtMAP65-1 in stomatal movement. ..

More Information

Publications20

  1. Zhou S, Chen Q, Li X, Li Y. MAP65-1 is required for the depolymerization and reorganization of cortical microtubules in the response to salt stress in Arabidopsis. Plant Sci. 2017;264:112-121 pubmed publisher
    ..MAP65-1 interacts with MPK6, not MPK3, affecting the MT's dynamic instability which is critical for plant salt-stress tolerance. ..
  2. Ho C, Lee Y, Kiyama L, Dinesh Kumar S, Liu B. Arabidopsis microtubule-associated protein MAP65-3 cross-links antiparallel microtubules toward their plus ends in the phragmoplast via its distinct C-terminal microtubule binding domain. Plant Cell. 2012;24:2071-85 pubmed publisher
    ..We conclude that MAP65-3 contains a distinct C-terminal MT binding site with a specific role in cross-linking antiparallel MTs toward their plus ends in the phragmoplast. ..
  3. Smertenko A, Kaloriti D, Chang H, Fiserova J, Opatrny Z, Hussey P. The C-terminal variable region specifies the dynamic properties of Arabidopsis microtubule-associated protein MAP65 isotypes. Plant Cell. 2008;20:3346-58 pubmed publisher
    ..Our data demonstrate that the C-terminal variable region, MTB2, determines the dynamic properties of individual isotypes and suggest that slower turnover is conditional for more efficient microtubule polymerization. ..
  4. Mao G, Chan J, Calder G, Doonan J, Lloyd C. Modulated targeting of GFP-AtMAP65-1 to central spindle microtubules during division. Plant J. 2005;43:469-78 pubmed
    ..It is concluded that AtMAP65-1 plays a role in the central spindle at anaphase to early cytokinesis but is not essential at the midline of the phragmoplast at later stages. ..
  5. Lucas J, Shaw S. MAP65-1 and MAP65-2 promote cell proliferation and axial growth in Arabidopsis roots. Plant J. 2012;71:454-63 pubmed publisher
    ..Our data indicate that MAP65-1 and MAP65-2 play a critical role in root growth by promoting cell proliferation and axial extension. ..
  6. Li H, Yuan M, Mao T. AtMAP65-1 binds to tubulin dimers to promote tubulin assembly. J Biochem Mol Biol. 2007;40:218-25 pubmed
    ..On the contrast, AtMAP65-1 deltaC495, which bound to microtubules but not to tubulin dimers, did not affect tubulin assembly. Our study suggested that AtMAP65-1 might promote tubulin assembly by binding to tubulin dimers in vivo. ..
  7. Smertenko A, Chang H, Sonobe S, Fenyk S, Weingartner M, Bögre L, et al. Control of the AtMAP65-1 interaction with microtubules through the cell cycle. J Cell Sci. 2006;119:3227-37 pubmed
    ..We conclude that normal metaphase spindle organisation and the transition to anaphase is dependent on inactivation of AtMAP65-1. ..
  8. Portran D, Zoccoler M, Gaillard J, Stoppin Mellet V, Neumann E, Arnal I, et al. MAP65/Ase1 promote microtubule flexibility. Mol Biol Cell. 2013;24:1964-73 pubmed publisher
    ..Taken together, these data provide new insights into how MAP65, by modifying MT mechanical properties, may regulate the formation of complex MT arrays. ..
  9. Beck M, Komis G, Muller J, Menzel D, Samaj J. Arabidopsis homologs of nucleus- and phragmoplast-localized kinase 2 and 3 and mitogen-activated protein kinase 4 are essential for microtubule organization. Plant Cell. 2010;22:755-71 pubmed publisher
    ..These data suggest that ANP2/ANP3, MPK4, and the microtubule-associated protein MAP65-1, a putative target of MPK4 signaling, are all essential for the proper organization of cortical microtubules in Arabidopsis epidermal cells. ..
  10. Mao T, Jin L, Li H, Liu B, Yuan M. Two microtubule-associated proteins of the Arabidopsis MAP65 family function differently on microtubules. Plant Physiol. 2005;138:654-62 pubmed
    ..It was concluded that these two MAP65 proteins were targeted to distinct sites, thus performing distinct functions in Arabidopsis cells. ..
  11. Boruc J, Weimer A, Stoppin Mellet V, Mylle E, Kosetsu K, Cedeño C, et al. Phosphorylation of MAP65-1 by Arabidopsis Aurora Kinases Is Required for Efficient Cell Cycle Progression. Plant Physiol. 2017;173:582-599 pubmed publisher
    ..Phospho-mutant analysis shows that Aurora contributes to the microtubule bundling capacity of MAP65-1 in concert with other mitotic kinases. ..