Gene Symbol: ISA1
Description: isoamylase 1
Alias: ARABIDOPSIS THALIANA ISOAMYLASE 1, ATISA1, T28M21.9, T28M21_9, isoamylase 1, isoamylase 1
Species: thale cress
Streb S, Delatte T, Umhang M, Eicke S, Schorderet M, Reinhardt D, et al
. Starch granule biosynthesis in Arabidopsis is abolished by removal of all debranching enzymes but restored by the subsequent removal of an endoamylase. Plant Cell. 2008;20:3448-66 pubmed publisher
..Quadruple mutants lacking all four DBE proteins (Isoamylase1 [ISA1], ISA2, and ISA3, and Limit-Dextrinase) are devoid of starch granules and instead accumulate highly branched ..
Delatte T, Trevisan M, Parker M, Zeeman S. Arabidopsis mutants Atisa1 and Atisa2 have identical phenotypes and lack the same multimeric isoamylase, which influences the branch point distribution of amylopectin during starch synthesis. Plant J. 2005;41:815-30 pubmed
..A reverse-genetic approach was used to knockout AtISA1, one of three genes in Arabidopsis encoding isoamylase-type debranching enzymes...
Facon M, Lin Q, Azzaz A, Hennen Bierwagen T, Myers A, Putaux J, et al
. Distinct functional properties of isoamylase-type starch debranching enzymes in monocot and dicot leaves. Plant Physiol. 2013;163:1363-75 pubmed publisher
..biosynthesis in chloroplast-containing organisms, as shown by the strict conservation of both catalytically active ISA1 and the noncatalytic homolog ISA2...
Wattebled F, Planchot V, Dong Y, Szydlowski N, Pontoire B, Devin A, et al
. Further evidence for the mandatory nature of polysaccharide debranching for the aggregation of semicrystalline starch and for overlapping functions of debranching enzymes in Arabidopsis leaves. Plant Physiol. 2008;148:1309-23 pubmed publisher
..isoforms of debranching enzymes are found in the genome of Arabidopsis (Arabidopsis thaliana): three isoamylases (ISA1, ISA2, and ISA3) and a pullulanase (PU1)...
Sundberg M, Pfister B, Fulton D, Bischof S, Delatte T, Eicke S, et al
. The heteromultimeric debranching enzyme involved in starch synthesis in Arabidopsis requires both isoamylase1 and isoamylase2 subunits for complex stability and activity. PLoS ONE. 2013;8:e75223 pubmed publisher
..Mutants of several species lacking the ISA1-subclass of isoamylase are impaired in amylopectin synthesis...
Streb S, Zeeman S. Replacement of the endogenous starch debranching enzymes ISA1 and ISA2 of Arabidopsis with the rice orthologs reveals a degree of functional conservation during starch synthesis. PLoS ONE. 2014;9:e92174 pubmed publisher
..of species-dependent differences in amylopectin structure by replacing the dicotyledonous Arabidopsis isoamylases (AtISA1 and AtISA2) with the monocotyledonous rice (Oryza sativa) isoforms...