Gene Symbol: HYL1
Description: dsRNA-binding domain-like superfamily protein
Alias: AtDRB1, DRB1, DSRNA-BINDING PROTEIN 1, F21M12.9, F21M12_9, HYPONASTIC LEAVES 1, dsRNA-binding domain-like superfamily protein
Species: thale cress
Products:     HYL1

Top Publications

  1. Tagami Y, Motose H, Watanabe Y. A dominant mutation in DCL1 suppresses the hyl1 mutant phenotype by promoting the processing of miRNA. RNA. 2009;15:450-8 pubmed publisher
    ..generated by DICER-LIKE1 (DCL1) with the assistance of a double-stranded RNA-binding protein, HYPONASTIC LEAVES1 (HYL1), in Arabidopsis. To achieve a better understanding of miRNA biogenesis, we isolated hyl1 suppressors...
  2. Eamens A, Smith N, Curtin S, Wang M, Waterhouse P. The Arabidopsis thaliana double-stranded RNA binding protein DRB1 directs guide strand selection from microRNA duplexes. RNA. 2009;15:2219-35 pubmed publisher
    ..thaliana (Arabidopsis), DICER-LIKE1 (DCL1) functions together with the double-stranded RNA binding protein (dsRBP), DRB1, to process microRNAs (miRNAs) from their precursor transcripts prior to their transfer to the RNA-induced ..
  3. Lian H, Li X, Liu Z, He Y. HYL1 is required for establishment of stamen architecture with four microsporangia in Arabidopsis. J Exp Bot. 2013;64:3397-410 pubmed publisher
    ..Here it is reported that HYPONASTIC LEAVES1 (HYL1), a general regulator of microRNA (miRNA) biogenesis, plays an essential role in establishing the stamen ..
  4. Fang Y, Spector D. Identification of nuclear dicing bodies containing proteins for microRNA biogenesis in living Arabidopsis plants. Curr Biol. 2007;17:818-23 pubmed
    ..protein Serrate (SE) and a double-stranded RNA (dsRNA) binding-domain protein, Hyponastic Leaves1 (HYL1)...
  5. Liu Z, Jia L, Wang H, He Y. HYL1 regulates the balance between adaxial and abaxial identity for leaf flattening via miRNA-mediated pathways. J Exp Bot. 2011;62:4367-81 pubmed publisher
    HYPONASTIC LEAVES1 (HYL1) is an important regulator of microRNA (miRNA) biogenesis. Incurvature of rosette leaves in loss-of-function mutants of HYL1 implicates the regulation of leaf flatness by HYL1 via miRNA pathways...
  6. Manavella P, Hagmann J, Ott F, Laubinger S, Franz M, Macek B, et al. Fast-forward genetics identifies plant CPL phosphatases as regulators of miRNA processing factor HYL1. Cell. 2012;151:859-870 pubmed publisher
    ..Processing accuracy and strand selection is greatly enhanced through the RNA binding protein HYPONASTIC LEAVES 1 (HYL1) and the zinc finger protein SERRATE (SE)...
  7. Wu F, Yu L, Cao W, Mao Y, Liu Z, He Y. The N-terminal double-stranded RNA binding domains of Arabidopsis HYPONASTIC LEAVES1 are sufficient for pre-microRNA processing. Plant Cell. 2007;19:914-25 pubmed
    Arabidopsis thaliana HYPONASTIC LEAVES1 (HYL1) is a microRNA (miRNA) biogenesis protein that contains two N-terminal double-stranded RNA binding domains (dsRBDs), a putative nuclear localization site (NLS), and a putative protein-protein ..
  8. Liu Q, Yan Q, Liu Y, Hong F, Sun Z, Shi L, et al. Complementation of HYPONASTIC LEAVES1 by double-strand RNA-binding domains of DICER-LIKE1 in nuclear dicing bodies. Plant Physiol. 2013;163:108-17 pubmed publisher
    ..mainly by the ribonuclease III-like enzyme DICER-LIKE1 (DCL1) and its specific partner, HYPONASTIC LEAVES1 (HYL1), a double-strand RNA-binding protein, both of which contain two double-strand RNA-binding domains (dsRBDs)...
  9. Kurihara Y, Takashi Y, Watanabe Y. The interaction between DCL1 and HYL1 is important for efficient and precise processing of pri-miRNA in plant microRNA biogenesis. RNA. 2006;12:206-12 pubmed
    ..the relationship between the Arabidopsis microRNA- (miRNA) producing enzyme DCL1 and the dsRNA binding protein HYL1. In the hyl1-2 mutant, the processing steps of miR163 biogenesis were partially impaired; increased accumulation of ..

More Information


  1. Hiraguri A, Itoh R, Kondo N, Nomura Y, Aizawa D, Murai Y, et al. Specific interactions between Dicer-like proteins and HYL1/DRB-family dsRNA-binding proteins in Arabidopsis thaliana. Plant Mol Biol. 2005;57:173-88 pubmed
    ..such gene products, each containing one or two dsRBMs: four Arabidopsis Dicer-like proteins (DCL1-4), Arabidopsis HYL1 and four of its homologs (DRB2, DRB4, DRB5 and OsDRB1)...
  2. Yang S, Chen H, Yang J, Machida S, Chua N, Yuan Y. Structure of Arabidopsis HYPONASTIC LEAVES1 and its molecular implications for miRNA processing. Structure. 2010;18:594-605 pubmed publisher
    The Arabidopsis HYPONASTIC LEAVES1 (HYL1) is a double-stranded RNA-binding protein that forms a complex with DICER-LIKE1 (DCL1) and SERRATE to facilitate processing of primary miRNAs into microRNAs (miRNAs)...
  3. Song L, Han M, Lesicka J, Fedoroff N. Arabidopsis primary microRNA processing proteins HYL1 and DCL1 define a nuclear body distinct from the Cajal body. Proc Natl Acad Sci U S A. 2007;104:5437-42 pubmed
    ..In the present work we investigated the role of the Arabidopsis nuclear RNA-binding protein HYL1 and the nuclear RNase III enzyme DCL1 in processing of primary miRNA (pri-miR171a)...
  4. Fujioka Y, Utsumi M, Ohba Y, Watanabe Y. Location of a possible miRNA processing site in SmD3/SmB nuclear bodies in Arabidopsis. Plant Cell Physiol. 2007;48:1243-53 pubmed
    ..In plants, it has been reported that Dicer-like 1 (DCL1), HYPONASTIC LEAVES1 (HYL1) and SERRATE (SE) are involved in the production of miRNAs...
  5. Yang L, Liu Z, Lu F, Dong A, Huang H. SERRATE is a novel nuclear regulator in primary microRNA processing in Arabidopsis. Plant J. 2006;47:841-50 pubmed
    ..Here we show that the se mutant exhibits some similar developmental abnormalities as the hyponastic leaves1 (hyl1) mutant...
  6. Qin H, Chen F, Huan X, Machida S, Song J, Yuan Y. Structure of the Arabidopsis thaliana DCL4 DUF283 domain reveals a noncanonical double-stranded RNA-binding fold for protein-protein interaction. RNA. 2010;16:474-81 pubmed publisher
    ..1 (dsRBD1) of Arabidopsis DRB4, whereas the DUF283 domain of DCL1 specifically binds to dsRBD2 of Arabidopsis HYL1. These data suggest a potential functional role of the Arabidopsis DUF283 domain in target selection in small RNA ..
  7. Vazquez F, Gasciolli V, Crété P, Vaucheret H. The nuclear dsRNA binding protein HYL1 is required for microRNA accumulation and plant development, but not posttranscriptional transgene silencing. Curr Biol. 2004;14:346-51 pubmed
    ..Here, we show that miRNA accumulation also depends on the activity of HYL1, a nuclear dsRNA binding protein...
  8. Li S, Yang X, Wu F, He Y. HYL1 controls the miR156-mediated juvenile phase of vegetative growth. J Exp Bot. 2012;63:2787-98 pubmed publisher
    b>HYL1 is an important regulator of microRNA (miRNA) biogenesis. A loss-of-function mutation of HYL1 causes the reduced accumulation of some miRNAs but fails to display the miRNA-deficient phenotypes of these miRNAs...
  9. Lu C, Fedoroff N. A mutation in the Arabidopsis HYL1 gene encoding a dsRNA binding protein affects responses to abscisic acid, auxin, and cytokinin. Plant Cell. 2000;12:2351-2366 pubmed
    ..We describe a pleiotropic recessive Arabidopsis transposon insertion mutation, designated hyponastic leaves (hyl1), that alters the plant's responses to several hormones...
  10. Allen E, Xie Z, Gustafson A, Carrington J. microRNA-directed phasing during trans-acting siRNA biogenesis in plants. Cell. 2005;121:207-21 pubmed
  11. Raghuram B, Sheikh A, Rustagi Y, Sinha A. MicroRNA biogenesis factor DRB1 is a phosphorylation target of mitogen activated protein kinase MPK3 in both rice and Arabidopsis. FEBS J. 2015;282:521-36 pubmed publisher
    MicroRNA (miRNA) biogenesis requires AtDRB1 (double-stranded RNA binding protein)/HYL1 (Hyponastic Leaves1) protein for processing and maturation of miRNA precursors...
  12. BaranauskÄ— S, MickutÄ— M, Plotnikova A, Finke A, Venclovas Ä, KlimaÅ¡auskas S, et al. Functional mapping of the plant small RNA methyltransferase: HEN1 physically interacts with HYL1 and DICER-LIKE 1 proteins. Nucleic Acids Res. 2015;43:2802-12 pubmed publisher
    ..mediated by its FK506-binding protein-like domain and R(2) domain, with the microRNA biogenesis protein HYL1. Furthermore, we find that HEN1 forms a complex with DICER-LIKE 1 (DCL1) ribonuclease, another key protein involved ..
  13. Jover Gil S, Candela H, Robles P, Aguilera V, Barrero J, Micol J, et al. The microRNA pathway genes AGO1, HEN1 and HYL1 participate in leaf proximal-distal, venation and stomatal patterning in Arabidopsis. Plant Cell Physiol. 2012;53:1322-33 pubmed publisher
    ..of incurvata8 (icu8), icu9 and icu15 has identified them as new loss-of-function alleles of the HYPONASTIC LEAVES1 (HYL1), ARGONAUTE1 (AGO1) and HUA ENHANCER1 (HEN1) genes, respectively, which encode known components of the microRNA ..
  14. Cho S, Ryu M, Poulsen C, Kim J, Oh T, Choi S, et al. HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana. FEBS Lett. 2017;591:1383-1393 pubmed publisher
    ..plants, the microprocessor minimally consists of three components: Dicer-like protein 1 (DCL1), HYPONASTIC LEAF 1 (HYL1), and SERRATE (SE)...
  15. Yang X, Ren W, Zhao Q, Zhang P, Wu F, He Y. Homodimerization of HYL1 ensures the correct selection of cleavage sites in primary miRNA. Nucleic Acids Res. 2014;42:12224-36 pubmed publisher
    MicroRNA (miRNA) plays an important role in the control of gene expression. HYPONASTIC LEAVES1 (HYL1) is a double-stranded RNA-binding protein that forms a complex with DICER-LIKE1 (DCL1) and SERRATE (SE) to process primary miRNA (pri-..
  16. Burdisso P, Milia F, Schapire A, Bologna N, Palatnik J, Rasia R. Structural determinants of Arabidopsis thaliana Hyponastic leaves 1 function in vivo. PLoS ONE. 2014;9:e113243 pubmed publisher
    ..In plants, the double stranded RNA binding protein Hyponastic Leaves 1 (HYL1) helps Dicer Like protein (DCL1) to achieve an efficient and precise excision of the miRNAs from ..
  17. Cho S, Ben Chaabane S, Shah P, Poulsen C, Yang S. COP1 E3 ligase protects HYL1 to retain microRNA biogenesis. Nat Commun. 2014;5:5867 pubmed publisher
    ..In cop1 mutants, the level of miRNAs is dramatically reduced because of the diminution of HYPONASTIC LEAVES 1 (HYL1), an RNA-binding protein required for precise miRNA processing...
  18. Su C, Li Z, Cheng J, Li L, Zhong S, Liu L, et al. The Protein Phosphatase 4 and SMEK1 Complex Dephosphorylates HYL1 to Promote miRNA Biogenesis by Antagonizing the MAPK Cascade in Arabidopsis. Dev Cell. 2017;41:527-539.e5 pubmed publisher
    ..HYL1 (Hyponastic Leaves 1), a core co-factor in plant miRNA biogenesis, is a short-lived phosphoprotein...
  19. Yan J, Wang P, Wang B, Hsu C, Tang K, Zhang H, et al. The SnRK2 kinases modulate miRNA accumulation in Arabidopsis. PLoS Genet. 2017;13:e1006753 pubmed publisher
    ..a processing complex that consists of the core components DICER-LIKE 1 (DCL1), SERRATE (SE) and HYPONASTIC LEAVES (HYL1)...
  20. Reis R, Hart Smith G, Eamens A, Wilkins M, Waterhouse P. MicroRNA Regulatory Mechanisms Play Different Roles in Arabidopsis. J Proteome Res. 2015;14:4743-51 pubmed publisher
    ..They act as key gene regulators for development and environmental adaptation, and Dicer-partnering proteins DRB1 and DRB2 govern which form of regulation plays the dominant role...
  21. Chen T, Cui P, Xiong L. The RNA-binding protein HOS5 and serine/arginine-rich proteins RS40 and RS41 participate in miRNA biogenesis in Arabidopsis. Nucleic Acids Res. 2015;43:8283-98 pubmed publisher
    ..Accuracy of the processing of pri-miRNA into mature miRNA in plants can be enhanced by SERRATE (SE) and HYPONASTIC LEAVES 1 (HYL1)...
  22. Zhang S, Liu Y, Yu B. PRL1, an RNA-binding protein, positively regulates the accumulation of miRNAs and siRNAs in Arabidopsis. PLoS Genet. 2014;10:e1004841 pubmed publisher
    ..Based on these results, we propose that CDC5 and PRL1 cooperatively regulate pri-miRNA levels, which results in their synergistic effects on miRNA accumulation, while they function together as a complex to enhance DCL1 activity. ..
  23. Li Z, Wang S, Cheng J, Su C, Zhong S, Liu Q, et al. Intron Lariat RNA Inhibits MicroRNA Biogenesis by Sequestering the Dicing Complex in Arabidopsis. PLoS Genet. 2016;12:e1006422 pubmed publisher
    ..of miRNA biogenesis genes or primary miRNAs (pri-miRNAs), but the association of pri-miRNAs with the DCL1/HYL1 dicing complex was impaired...
  24. Liu Y, Liu Q, Yan Q, Shi L, Fang Y. Nucleolus-tethering system (NoTS) reveals that assembly of photobodies follows a self-organization model. Mol Biol Cell. 2014;25:1366-73 pubmed publisher
  25. Pouch Pélissier M, Pélissier T, Elmayan T, Vaucheret H, Boko D, Jantsch M, et al. SINE RNA induces severe developmental defects in Arabidopsis thaliana and interacts with HYL1 (DRB1), a key member of the DCL1 complex. PLoS Genet. 2008;4:e1000096 pubmed publisher
    ..We show that SB1 RNA interacts with HYL1 (DRB1), a double-stranded RNA-binding protein (dsRBP) that associates with the Dicer homologue DCL1 to produce ..
  26. Szarzynska B, Sobkowiak L, Pant B, Balazadeh S, Scheible W, Mueller Roeber B, et al. Gene structures and processing of Arabidopsis thaliana HYL1-dependent pri-miRNAs. Nucleic Acids Res. 2009;37:3083-93 pubmed publisher
    Arabidopsis thaliana HYL1 is a nuclear double-stranded RNA-binding protein involved in the maturation of pri-miRNAs...