Gene Symbol: HEN1
Description: double-stranded RNA binding protein-related / DsRBD protein-like protein
Alias: CORYMBOSA 2, CRM2, HUA ENHANCER 1, T13K14.70, T13K14_70, double-stranded RNA binding protein-related / DsRBD protein-like protein
Species: thale cress
Products:     HEN1

Top Publications

  1. Yu B, Yang Z, Li J, Minakhina S, Yang M, Padgett R, et al. Methylation as a crucial step in plant microRNA biogenesis. Science. 2005;307:932-5 pubmed
    ..Whereas methylation of rRNAs depends on guide RNAs, the methyltransferase protein HEN1 is sufficient to methylate miRNA/miRNA* duplexes...
  2. Allen E, Xie Z, Gustafson A, Carrington J. microRNA-directed phasing during trans-acting siRNA biogenesis in plants. Cell. 2005;121:207-21 pubmed
  3. Chen X, Liu J, Cheng Y, Jia D. HEN1 functions pleiotropically in Arabidopsis development and acts in C function in the flower. Development. 2002;129:1085-94 pubmed
    ..We identified a new gene, HUA ENHANCER 1 (HEN1) from a sensitized genetic screen in the hua1-1 hua2-1 background that is compromised in floral homeotic ..
  4. Plotnikova A, Baranauskė S, Osipenko A, Klimasauskas S, Vilkaitis G. Mechanistic insights into small RNA recognition and modification by the HEN1 methyltransferase. Biochem J. 2013;453:281-90 pubmed publisher
    The HEN1 methyltransferase from Arabidopsis thaliana modifies the 3'-terminal nucleotides of small regulatory RNAs...
  5. Huang Y, Ji L, Huang Q, Vassylyev D, Chen X, Ma J. Structural insights into mechanisms of the small RNA methyltransferase HEN1. Nature. 2009;461:823-7 pubmed publisher
    ..A conserved S-adenosyl-l-methionine-dependent RNA methyltransferase, HUA ENHANCER 1 (HEN1), and its homologues are responsible for this specific modification. Here we report the 3...
  6. Li J, Yang Z, Yu B, Liu J, Chen X. Methylation protects miRNAs and siRNAs from a 3'-end uridylation activity in Arabidopsis. Curr Biol. 2005;15:1501-7 pubmed
    ..In addition to a Dicer homolog, DCL1, the biogenesis of miRNAs in Arabidopsis requires another protein, HEN1. miRNAs are reduced in abundance and increased in size in hen1 mutants...
  7. Boutet S, Vazquez F, Liu J, Beclin C, Fagard M, Gratias A, et al. Arabidopsis HEN1: a genetic link between endogenous miRNA controlling development and siRNA controlling transgene silencing and virus resistance. Curr Biol. 2003;13:843-8 pubmed
    ..The SGS2/SDE1 protein contributes to produce transgene siRNA, while DCL1 and HEN1 contribute to endogenous miRNA accumulation...
  8. Fang Y, Spector D. Identification of nuclear dicing bodies containing proteins for microRNA biogenesis in living Arabidopsis plants. Curr Biol. 2007;17:818-23 pubmed
    ..Furthermore, plant miRNAs are methylated by Hua Enhancer (HEN1) at their 3' ends and loaded onto Argonaute1 (AGO1)...
  9. Vilkaitis G, Plotnikova A, Klimasauskas S. Kinetic and functional analysis of the small RNA methyltransferase HEN1: the catalytic domain is essential for preferential modification of duplex RNA. RNA. 2010;16:1935-42 pubmed publisher
    The HEN1 RNA methyltransferase from Arabidopsis thaliana catalyzes S-adenosyl-L-methionine (AdoMet)-dependent 2'-O-methylation at the 3'-termini of small double-stranded RNAs and is a crucial factor in the biogenesis of plant small ..

More Information


  1. Zhang X, Zhang X, Singh J, Li D, Qu F. Temperature-dependent survival of Turnip crinkle virus-infected arabidopsis plants relies on an RNA silencing-based defense that requires dcl2, AGO2, and HEN1. J Virol. 2012;86:6847-54 pubmed publisher
    ..Arabidopsis, plants containing loss-of-function mutations within the Dicer-like 2 (DCL2), Argonaute 2 (AGO2), and HEN1 RNA methyltransferase genes died of TCV infection, whereas the wild-type Col-0 plants survived to produce viable ..
  2. Yu B, Bi L, Zhai J, Agarwal M, Li S, Wu Q, et al. siRNAs compete with miRNAs for methylation by HEN1 in Arabidopsis. Nucleic Acids Res. 2010;38:5844-50 pubmed publisher
    ..This methyl group is post-synthetically added by the methyltransferase protein HEN1 and protects small RNAs from enzymatic activities that target the 3'-OH...
  3. Wang X, Zhang S, Dou Y, Zhang C, Chen X, Yu B, et al. Synergistic and independent actions of multiple terminal nucleotidyl transferases in the 3' tailing of small RNAs in Arabidopsis. PLoS Genet. 2015;11:e1005091 pubmed publisher
    ..elegans are subject to HEN1 mediated 3' terminal 2'-O-methylation...
  4. Suzuki M, Takahashi T, Komeda Y. Formation of corymb-like inflorescences due to delay in bolting and flower development in the corymbosa2 mutant of Arabidopsis. Plant Cell Physiol. 2002;43:298-306 pubmed
    ..We report here the characterization of another mutant with a corymb-like inflorescence, named corymbosa2 (crm2), and the isolation of the CRM2 gene...
  5. Zhai J, Zhao Y, Simon S, Huang S, Petsch K, Arikit S, et al. Plant microRNAs display differential 3' truncation and tailing modifications that are ARGONAUTE1 dependent and conserved across species. Plant Cell. 2013;25:2417-28 pubmed publisher
    Plant small RNAs are 3' methylated by the methyltransferase HUA1 ENHANCER1 (HEN1)...
  6. Tsai H, Li Y, Hsieh W, Lin M, Ahn J, Wu S. HUA ENHANCER1 is involved in posttranscriptional regulation of positive and negative regulators in Arabidopsis photomorphogenesis. Plant Cell. 2014;26:2858-72 pubmed publisher
    ..Arabidopsis carrying a mutation in HUA ENHANCER1 (HEN1), required for stabilization of small regulatory RNAs, showed defects in multiple aspects of photomorphogenic and ..
  7. Jover Gil S, Candela H, Robles P, Aguilera V, Barrero J, Micol J, et al. The microRNA pathway genes AGO1, HEN1 and HYL1 participate in leaf proximal-distal, venation and stomatal patterning in Arabidopsis. Plant Cell Physiol. 2012;53:1322-33 pubmed publisher
    ..them as new loss-of-function alleles of the HYPONASTIC LEAVES1 (HYL1), ARGONAUTE1 (AGO1) and HUA ENHANCER1 (HEN1) genes, respectively, which encode known components of the microRNA pathway...
  8. He X, Hsu Y, Pontes O, Zhu J, Lu J, Bressan R, et al. NRPD4, a protein related to the RPB4 subunit of RNA polymerase II, is a component of RNA polymerases IV and V and is required for RNA-directed DNA methylation. Genes Dev. 2009;23:318-30 pubmed publisher
    ..we identified a new component (referred to as RDM2) as well as seven known components (NRPD1, NRPE1, NRPD2a, AGO4, HEN1, DRD1, and HDA6) of the RdDM pathway...
  9. BaranauskÄ— S, MickutÄ— M, Plotnikova A, Finke A, Venclovas Ä, KlimaÅ¡auskas S, et al. Functional mapping of the plant small RNA methyltransferase: HEN1 physically interacts with HYL1 and DICER-LIKE 1 proteins. Nucleic Acids Res. 2015;43:2802-12 pubmed publisher
    ..In Arabidopsis thaliana this reaction is carried out by a multidomain AdoMet-dependent 2'-O-methyltransferase HEN1. Using deletion and structure-guided mutational analysis, we show that the double-stranded RNA-binding domains R(1) ..
  10. Jamous R, Boonrod K, Fuellgrabe M, Ali Shtayeh M, Krczal G, Wassenegger M. The helper component-proteinase of the Zucchini yellow mosaic virus inhibits the Hua Enhancer 1 methyltransferase activity in vitro. J Gen Virol. 2011;92:2222-6 pubmed publisher
    ..HC-Pro could also exhibit other suppressor activities. HC-Pro may inhibit the Hua Enhancer 1 (HEN1) activity...
  11. Dunoyer P, Himber C, Ruiz Ferrer V, Alioua A, Voinnet O. Intra- and intercellular RNA interference in Arabidopsis thaliana requires components of the microRNA and heterochromatic silencing pathways. Nat Genet. 2007;39:848-56 pubmed
    ..Finally, we show that the genetics of RNAi is distinct from that of antiviral silencing and propose that this experimental silencing pathway has a direct endogenous plant counterpart. ..
  12. Blevins T, Rajeswaran R, Shivaprasad P, Beknazariants D, Si Ammour A, Park H, et al. Four plant Dicers mediate viral small RNA biogenesis and DNA virus induced silencing. Nucleic Acids Res. 2006;34:6233-46 pubmed
    ..While the four DCLs are partially redundant for CaLCuV-induced mRNA degradation, DCL4 in conjunction with RDR6 and HEN1 specifically facilitates extensive virus-induced silencing in new growth...