GSH1

Summary

Gene Symbol: GSH1
Description: glutamate-cysteine ligase
Alias: ATECS1, AtGSH1, CAD2, CADMIUM SENSITIVE 2, F7H19.290, F7H19_290, GAMMA-GLUTAMYL-CYSTEINE SYNTHETASE, GAMMA-GLUTAMYLCYSTEINE SYNTHETASE, GLUTAMATE-CYSTEINE LIGASE, GSHA, PAD2, PHYTOALEXIN DEFICIENT 2, RML1, ROOT MERISTEMLESS 1, glutamate-cysteine ligase, glutamate-cysteine ligase
Species: thale cress

Top Publications

  1. Jez J, Cahoon R, Chen S. Arabidopsis thaliana glutamate-cysteine ligase: functional properties, kinetic mechanism, and regulation of activity. J Biol Chem. 2004;279:33463-70 pubmed
    ..These results explain the reported posttranslational change in AtGCL activity in response to oxidative stress. ..
  2. Sasaki Sekimoto Y, Taki N, Obayashi T, Aono M, Matsumoto F, Sakurai N, et al. Coordinated activation of metabolic pathways for antioxidants and defence compounds by jasmonates and their roles in stress tolerance in Arabidopsis. Plant J. 2005;44:653-68 pubmed
    ..Compared with the wild type, opr3 mutants were more sensitive to O3 exposure. These results suggest that the coordinated activation of the metabolic pathways mediated by jasmonates provides resistance to environmental stresses. ..
  3. Dubreuil Maurizi C, Vitecek J, Marty L, Branciard L, Frettinger P, Wendehenne D, et al. Glutathione deficiency of the Arabidopsis mutant pad2-1 affects oxidative stress-related events, defense gene expression, and the hypersensitive response. Plant Physiol. 2011;157:2000-12 pubmed publisher
    The Arabidopsis (Arabidopsis thaliana) phytoalexin-deficient mutant pad2-1 displays enhanced susceptibility to a broad range of pathogens and herbivorous insects that correlates with deficiencies in the production of camalexin, indole ..
  4. Parisy V, Poinssot B, Owsianowski L, Buchala A, Glazebrook J, Mauch F. Identification of PAD2 as a gamma-glutamylcysteine synthetase highlights the importance of glutathione in disease resistance of Arabidopsis. Plant J. 2007;49:159-72 pubmed
    ..was used to map the mutation to the gene At4g23100, which encodes gamma-glutamylcysteine synthetase (gamma-ECS, GSH1). GSH1 catalyzes the first committed step of glutathione (GSH) biosynthesis...
  5. Clay N, Adio A, Denoux C, Jander G, Ausubel F. Glucosinolate metabolites required for an Arabidopsis innate immune response. Science. 2009;323:95-101 pubmed publisher
    ..Our study shows that well-studied plant metabolites, previously identified as important in avoiding damage by herbivores, are also required as a component of the plant defense response against microbial pathogens...
  6. Hatano Iwasaki A, Ogawa K. Overexpression of GSH1 gene mimics transcriptional response to low temperature during seed vernalization treatment of Arabidopsis. Plant Cell Physiol. 2012;53:1195-203 pubmed publisher
    ..thaliana. A similar pattern was observed at 22°C in transgenic (35S-GSH1) plants overexpressing the ?-glutamylcysteine synthetase gene GSH1, coding for an enzyme limiting GSH biosynthesis, ..
  7. Howden R, Andersen C, Goldsbrough P, Cobbett C. A cadmium-sensitive, glutathione-deficient mutant of Arabidopsis thaliana. Plant Physiol. 1995;107:1067-73 pubmed
    ..A new cadmium-sensitive mutant affected at a second locus, cad2, has been identified using this phenotype...
  8. Glazebrook J, Ausubel F. Isolation of phytoalexin-deficient mutants of Arabidopsis thaliana and characterization of their interactions with bacterial pathogens. Proc Natl Acad Sci U S A. 1994;91:8955-9 pubmed
    ..thaliana ecotype Columbia that were phytoalexin deficient (pad mutants). The mutations pad1, pad2, and pad3 were found to be recessive alleles of three different genes...
  9. Cobbett C, May M, Howden R, Rolls B. The glutathione-deficient, cadmium-sensitive mutant, cad2-1, of Arabidopsis thaliana is deficient in gamma-glutamylcysteine synthetase. Plant J. 1998;16:73-8 pubmed
    This paper reports that the glutathione (GSH)-deficient mutant, cad2-1, of Arabidopsis is deficient in the first enzyme in the pathway of GSH biosynthesis, gamma-glutamylcysteine synthetase (GCS)...
  10. Wachter A, Wolf S, Steininger H, Bogs J, Rausch T. Differential targeting of GSH1 and GSH2 is achieved by multiple transcription initiation: implications for the compartmentation of glutathione biosynthesis in the Brassicaceae. Plant J. 2005;41:15-30 pubmed
    The genome of Arabidopsis thaliana reveals that in this species the enzymes of glutathione biosynthesis, GSH1 and GSH2, are encoded by single genes...

Detail Information

Publications15

  1. Jez J, Cahoon R, Chen S. Arabidopsis thaliana glutamate-cysteine ligase: functional properties, kinetic mechanism, and regulation of activity. J Biol Chem. 2004;279:33463-70 pubmed
    ..These results explain the reported posttranslational change in AtGCL activity in response to oxidative stress. ..
  2. Sasaki Sekimoto Y, Taki N, Obayashi T, Aono M, Matsumoto F, Sakurai N, et al. Coordinated activation of metabolic pathways for antioxidants and defence compounds by jasmonates and their roles in stress tolerance in Arabidopsis. Plant J. 2005;44:653-68 pubmed
    ..Compared with the wild type, opr3 mutants were more sensitive to O3 exposure. These results suggest that the coordinated activation of the metabolic pathways mediated by jasmonates provides resistance to environmental stresses. ..
  3. Dubreuil Maurizi C, Vitecek J, Marty L, Branciard L, Frettinger P, Wendehenne D, et al. Glutathione deficiency of the Arabidopsis mutant pad2-1 affects oxidative stress-related events, defense gene expression, and the hypersensitive response. Plant Physiol. 2011;157:2000-12 pubmed publisher
    The Arabidopsis (Arabidopsis thaliana) phytoalexin-deficient mutant pad2-1 displays enhanced susceptibility to a broad range of pathogens and herbivorous insects that correlates with deficiencies in the production of camalexin, indole ..
  4. Parisy V, Poinssot B, Owsianowski L, Buchala A, Glazebrook J, Mauch F. Identification of PAD2 as a gamma-glutamylcysteine synthetase highlights the importance of glutathione in disease resistance of Arabidopsis. Plant J. 2007;49:159-72 pubmed
    ..was used to map the mutation to the gene At4g23100, which encodes gamma-glutamylcysteine synthetase (gamma-ECS, GSH1). GSH1 catalyzes the first committed step of glutathione (GSH) biosynthesis...
  5. Clay N, Adio A, Denoux C, Jander G, Ausubel F. Glucosinolate metabolites required for an Arabidopsis innate immune response. Science. 2009;323:95-101 pubmed publisher
    ..Our study shows that well-studied plant metabolites, previously identified as important in avoiding damage by herbivores, are also required as a component of the plant defense response against microbial pathogens...
  6. Hatano Iwasaki A, Ogawa K. Overexpression of GSH1 gene mimics transcriptional response to low temperature during seed vernalization treatment of Arabidopsis. Plant Cell Physiol. 2012;53:1195-203 pubmed publisher
    ..thaliana. A similar pattern was observed at 22°C in transgenic (35S-GSH1) plants overexpressing the ?-glutamylcysteine synthetase gene GSH1, coding for an enzyme limiting GSH biosynthesis, ..
  7. Howden R, Andersen C, Goldsbrough P, Cobbett C. A cadmium-sensitive, glutathione-deficient mutant of Arabidopsis thaliana. Plant Physiol. 1995;107:1067-73 pubmed
    ..A new cadmium-sensitive mutant affected at a second locus, cad2, has been identified using this phenotype...
  8. Glazebrook J, Ausubel F. Isolation of phytoalexin-deficient mutants of Arabidopsis thaliana and characterization of their interactions with bacterial pathogens. Proc Natl Acad Sci U S A. 1994;91:8955-9 pubmed
    ..thaliana ecotype Columbia that were phytoalexin deficient (pad mutants). The mutations pad1, pad2, and pad3 were found to be recessive alleles of three different genes...
  9. Cobbett C, May M, Howden R, Rolls B. The glutathione-deficient, cadmium-sensitive mutant, cad2-1, of Arabidopsis thaliana is deficient in gamma-glutamylcysteine synthetase. Plant J. 1998;16:73-8 pubmed
    This paper reports that the glutathione (GSH)-deficient mutant, cad2-1, of Arabidopsis is deficient in the first enzyme in the pathway of GSH biosynthesis, gamma-glutamylcysteine synthetase (GCS)...
  10. Wachter A, Wolf S, Steininger H, Bogs J, Rausch T. Differential targeting of GSH1 and GSH2 is achieved by multiple transcription initiation: implications for the compartmentation of glutathione biosynthesis in the Brassicaceae. Plant J. 2005;41:15-30 pubmed
    The genome of Arabidopsis thaliana reveals that in this species the enzymes of glutathione biosynthesis, GSH1 and GSH2, are encoded by single genes...
  11. Larkindale J, Hall J, Knight M, Vierling E. Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance. Plant Physiol. 2005;138:882-97 pubmed
    ..Ethylene signaling mutants (ein2 and etr1) and reactive oxygen metabolism mutants (vtc1, vtc2, npq1, and cad2) were more defective in basal than acquired thermotolerance, especially under high light...
  12. Ogawa K, Tasaka Y, Mino M, Tanaka Y, Iwabuchi M. Association of glutathione with flowering in Arabidopsis thaliana. Plant Cell Physiol. 2001;42:524-30 pubmed
    ..An A. thaliana mutant, cad2-1, which has a defect in GSH biosynthesis also exhibited late flowering...
  13. Hicks L, Cahoon R, Bonner E, Rivard R, Sheffield J, Jez J. Thiol-based regulation of redox-active glutamate-cysteine ligase from Arabidopsis thaliana. Plant Cell. 2007;19:2653-61 pubmed
  14. Schlaeppi K, Bodenhausen N, Buchala A, Mauch F, Reymond P. The glutathione-deficient mutant pad2-1 accumulates lower amounts of glucosinolates and is more susceptible to the insect herbivore Spodoptera littoralis. Plant J. 2008;55:774-86 pubmed publisher
    ..The Arabidopsis mutant pad2-1 has reduced levels of the phytoalexin camalexin and is known for its increased susceptibility to fungal and ..
  15. Shanmugam V, Tsednee M, Yeh K. ZINC TOLERANCE INDUCED BY IRON 1 reveals the importance of glutathione in the cross-homeostasis between zinc and iron in Arabidopsis thaliana. Plant J. 2012;69:1006-17 pubmed publisher
    ..we identified that zir1 has a mutation of glutamate to lysine at position 385 on ?-glutamylcysteine synthetase (GSH1), the enzyme involved in glutathione biosynthesis...