Gene Symbol: GRF6
Description: G-box regulating factor 6
Alias: 14-3-3 PROTEIN G-BOX FACTOR14 LAMBDA, 14-3-3LAMBDA, 14-3-3lambda, AFT1, F12B17.200, F12B17_200, G-box regulating factor 6, GF14 LAMBDA, G-box regulating factor 6
Species: thale cress
Products:     GRF6

Top Publications

  1. Latz A, Becker D, Hekman M, Muller T, Beyhl D, Marten I, et al. TPK1, a Ca(2+)-regulated Arabidopsis vacuole two-pore K(+) channel is activated by 14-3-3 proteins. Plant J. 2007;52:449-59 pubmed
    ..Following in planta expression TPK1 and GRF6 co-localize at the vacuolar membrane...
  2. Gampala S, Kim T, He J, Tang W, Deng Z, Bai M, et al. An essential role for 14-3-3 proteins in brassinosteroid signal transduction in Arabidopsis. Dev Cell. 2007;13:177-89 pubmed
    ..This study demonstrates that multiple mechanisms are required for BR regulation of gene expression and plant growth. ..
  3. Tseng T, Whippo C, Hangarter R, Briggs W. The role of a 14-3-3 protein in stomatal opening mediated by PHOT2 in Arabidopsis. Plant Cell. 2012;24:1114-26 pubmed publisher
    ..The results highlight the strict specificity of phototropin-mediated signal transduction pathways. ..
  4. Kanamaru K, Wang R, Su W, Crawford N. Ser-534 in the hinge 1 region of Arabidopsis nitrate reductase is conditionally required for binding of 14-3-3 proteins and in vitro inhibition. J Biol Chem. 1999;274:4160-5 pubmed
  5. Zhang H, Wang J, Nickel U, Allen R, Goodman H. Cloning and expression of an Arabidopsis gene encoding a putative peroxisomal ascorbate peroxidase. Plant Mol Biol. 1997;34:967-71 pubmed
    An Arabidopsis 14-3-3 protein, AFT1, was used as a 'bait' in the two-hybrid system to identify its interacting proteins. One of the candidate proteins, APX3, was identified as a putative peroxisomal membrane-bound ascorbate peroxidase...
  6. Abarca D, Madueno F, Martínez Zapater J, Salinas J. Dimerization of Arabidopsis 14-3-3 proteins: structural requirements within the N-terminal domain and effect of calcium. FEBS Lett. 1999;462:377-82 pubmed
    ..Millimolar concentrations of calcium exert a negative, dose-dependent effect that involves the C-terminal domain of RCI14A and might modulate interactions with other cellular components or among Arabidopsis 14-3-3 isoforms. ..
  7. Moreno J, Shyu C, Campos M, Patel L, Chung H, Yao J, et al. Negative feedback control of jasmonate signaling by an alternative splice variant of JAZ10. Plant Physiol. 2013;162:1006-17 pubmed publisher
    ..4 links transcription factors to a corepressor complex and suggest how JA-induced transcription and alternative splicing of JAZ10 premessenger RNA creates a regulatory circuit to attenuate JA responses. ..
  8. Sullivan S, Thomson C, Kaiserli E, Christie J. Interaction specificity of Arabidopsis 14-3-3 proteins with phototropin receptor kinases. FEBS Lett. 2009;583:2187-93 pubmed publisher
    ..No 14-3-3 binding was detected for Arabidopsis phot2, suggesting that 14-3-3 proteins are specific to phot1 signalling. ..
  9. Sehnke P, Henry R, Cline K, Ferl R. Interaction of a plant 14-3-3 protein with the signal peptide of a thylakoid-targeted chloroplast precursor protein and the presence of 14-3-3 isoforms in the chloroplast stroma. Plant Physiol. 2000;122:235-42 pubmed
    ..These isoforms represent two distinct phylogenetic 14-3-3 groupings. These data suggest a novel interorganellar role for these phylogenetically distinct 14-3-3 proteins. ..

More Information


  1. Sepúlveda García E, Rocha Sosa M. The Arabidopsis F-box protein AtFBS1 interacts with 14-3-3 proteins. Plant Sci. 2012;195:36-47 pubmed publisher
    ..AICAR also affects Cullin1 (CUL1) modification by RUB1, which would provide an additional element to the effect of this compound on AtFBS1 stability. ..
  2. Yang X, Wang W, Coleman M, Orgil U, Feng J, Ma X, et al. Arabidopsis 14-3-3 lambda is a positive regulator of RPW8-mediated disease resistance. Plant J. 2009;60:539-50 pubmed publisher
    ..The results from this study suggest that GF14lambda may positively regulate the RPW8.2 resistance function and play a role in enhancing basal resistance in Arabidopsis. ..
  3. Liu Z, Jia Y, Ding Y, Shi Y, Li Z, Guo Y, et al. Plasma Membrane CRPK1-Mediated Phosphorylation of 14-3-3 Proteins Induces Their Nuclear Import to Fine-Tune CBF Signaling during Cold Response. Mol Cell. 2017;66:117-128.e5 pubmed publisher
    ..Thus, our study reveals that the CRPK1-14-3-3 module transduces the cold signal from the plasma membrane to the nucleus to modulate CBF stability, which ensures a faithfully adjusted response to cold stress of plants. ..
  4. Zhang H, Wang J, Goodman H. An Arabidopsis gene encoding a putative 14-3-3-interacting protein, caffeic acid/5-hydroxyferulic acid O-methyltransferase. Biochim Biophys Acta. 1997;1353:199-202 pubmed
    b>AFT1, a 14-3-3 protein from Arabidopsis thaliana, was used as a 'bait' in the two-hybrid system to identify its interacting proteins...
  5. Gao J, van Kleeff P, Oecking C, Li K, Erban A, Kopka J, et al. Light modulated activity of root alkaline/neutral invertase involves the interaction with 14-3-3 proteins. Plant J. 2014;80:785-96 pubmed publisher
    ..The nature of the light-induced signal that travels from the shoot to root and the question whether this signal is transmitted via cytosolic Ca(++) changes that activate calcium-dependent kinases, await further study. ..
  6. Zhao S, Zhao Y, Guo Y. 14-3-3 λ protein interacts with ADF1 to regulate actin cytoskeleton dynamics in Arabidopsis. Sci China Life Sci. 2015;58:1142-50 pubmed publisher
    ..Consistent with these observations, the actin filaments were more stable in 14-3-3 λ mutant. Our results indicate that 14-3-3 λ protein mediates F-actin dynamics possibly through inhibiting ADF1 phosphorylation in vivo. ..
  7. Tang W, Yuan M, Wang R, Yang Y, Wang C, Oses Prieto J, et al. PP2A activates brassinosteroid-responsive gene expression and plant growth by dephosphorylating BZR1. Nat Cell Biol. 2011;13:124-31 pubmed publisher
  8. van Kleeff P, Jaspert N, Li K, Rauch S, Oecking C, de Boer A. Higher order Arabidopsis 14-3-3 mutants show 14-3-3 involvement in primary root growth both under control and abiotic stress conditions. J Exp Bot. 2014;65:5877-88 pubmed publisher
  9. Rienties I, Vink J, Borst J, Russinova E, de Vries S. The Arabidopsis SERK1 protein interacts with the AAA-ATPase AtCDC48, the 14-3-3 protein GF14lambda and the PP2C phosphatase KAPP. Planta. 2005;221:394-405 pubmed
    ..In yeast, AtCDC48 interacts with GF14lambda and with the PP2C phosphatase KAPP. In plant protoplasts AtSERK1 interacts with GF14lambda. ..
  10. Yasuda S, Sato T, Maekawa S, Aoyama S, Fukao Y, Yamaguchi J. Phosphorylation of Arabidopsis ubiquitin ligase ATL31 is critical for plant carbon/nitrogen nutrient balance response and controls the stability of 14-3-3 proteins. J Biol Chem. 2014;289:15179-93 pubmed publisher
    ..Together, these results demonstrate that the physiological role of phosphorylation at 14-3-3 binding sites on ATL31 is to modulate the binding ability and stability of 14-3-3 proteins to control plant C/N-nutrient response...