Gene Symbol: FIE
Description: Transducin/WD40 repeat-like superfamily protein
Species: thale cress

Top Publications

  1. Katz A, Oliva M, Mosquna A, Hakim O, Ohad N. FIE and CURLY LEAF polycomb proteins interact in the regulation of homeobox gene expression during sporophyte development. Plant J. 2004;37:707-19 pubmed
    The Arabidopsis FERTILIZATION-INDEPENDENT ENDOSPERM (FIE) polycomb group (PcG) protein, a WD40 homologue of Drosophila extra sex comb (ESC), regulates endosperm and embryo development and represses flowering during embryo and seedling ..
  2. Chanvivattana Y, Bishopp A, Schubert D, Stock C, Moon Y, Sung Z, et al. Interaction of Polycomb-group proteins controlling flowering in Arabidopsis. Development. 2004;131:5263-76 pubmed
  3. Luo M, Bilodeau P, Dennis E, Peacock W, Chaudhury A. Expression and parent-of-origin effects for FIS2, MEA, and FIE in the endosperm and embryo of developing Arabidopsis seeds. Proc Natl Acad Sci U S A. 2000;97:10637-42 pubmed
    The promoters of MEA (FIS1), FIS2, and FIE (FIS3), genes that repress seed development in the absence of pollination, were fused to beta-glucuronidase (GUS) to study their activity pattern...
  4. Wood C, Robertson M, Tanner G, Peacock W, Dennis E, Helliwell C. The Arabidopsis thaliana vernalization response requires a polycomb-like protein complex that also includes VERNALIZATION INSENSITIVE 3. Proc Natl Acad Sci U S A. 2006;103:14631-6 pubmed planta that VRN2 and VIN3 are part of a large protein complex that can include the PcG proteins FERTILIZATION INDEPENDENT ENDOSPERM, CURLY LEAF, and SWINGER...
  5. Lodha M, Marco C, Timmermans M. The ASYMMETRIC LEAVES complex maintains repression of KNOX homeobox genes via direct recruitment of Polycomb-repressive complex2. Genes Dev. 2013;27:596-601 pubmed publisher
    ..Combined with recent studies in mammals, our findings reveal a conserved paradigm to epigenetically regulate homeobox gene expression during development. ..
  6. Derkacheva M, Steinbach Y, Wildhaber T, Mozgová I, Mahrez W, Nanni P, et al. Arabidopsis MSI1 connects LHP1 to PRC2 complexes. EMBO J. 2013;32:2073-85 pubmed publisher
    ..The LHP1-MSI1 interaction forms a positive feedback loop to recruit PRC2 to chromatin that carries H3K27me3. Consequently, this can provide a mechanism for the faithful inheritance of local epigenetic information through replication. ..
  7. Bracha Drori K, Shichrur K, Katz A, Oliva M, Angelovici R, Yalovsky S, et al. Detection of protein-protein interactions in plants using bimolecular fluorescence complementation. Plant J. 2004;40:419-27 pubmed
    ..Arabidopsis protein farnesyltransferase (PFT), and (ii) the polycomb proteins, FERTILIZATION-INDEPENDENT ENDOSPERM (FIE) and MEDEA (MEA)...
  8. Vinkenoog R, Spielman M, Adams S, Fischer R, Dickinson H, Scott R. Hypomethylation promotes autonomous endosperm development and rescues postfertilization lethality in fie mutants. Plant Cell. 2000;12:2271-82 pubmed
    In most flowering plants, fertilization is necessary for development of the central cell into endosperm, but in the fie-1 mutant of Arabidopsis, the central cell can proliferate autonomously...
  9. Spillane C, MacDougall C, Stock C, Kohler C, Vielle Calzada J, Nunes S, et al. Interaction of the Arabidopsis polycomb group proteins FIE and MEA mediates their common phenotypes. Curr Biol. 2000;10:1535-8 pubmed
    ..The FIS genes FERTILISATION INDEPENDENT ENDOSPERM (FIE) and MEDEA (MEA) encode homologs of animal Polycomb group (Pc-G) proteins, transcriptional regulators that modify ..

More Information


  1. Kohler C, Hennig L, Bouveret R, Gheyselinck J, Grossniklaus U, Gruissem W. Arabidopsis MSI1 is a component of the MEA/FIE Polycomb group complex and required for seed development. EMBO J. 2003;22:4804-14 pubmed
    ..the absence of fertilization requires the Polycomb group proteins MEDEA (MEA), FERTILIZATION-INDEPENDENT ENDOSPERM (FIE) and FERTILIZATION-INDEPENDENT SEED2...
  2. Yadegari R, Kinoshita T, Lotan O, Cohen G, Choi Y, Katz A, et al. Mutations in the FIE and MEA genes that encode interacting polycomb proteins cause parent-of-origin effects on seed development by distinct mechanisms. Plant Cell. 2000;12:2367-2382 pubmed
    ..The FERTILIZATION-INDEPENDENT ENDOSPERM (FIE) and MEDEA (MEA) genes encode WD and SET domain polycomb proteins, respectively...
  3. Mosquna A, Katz A, Shochat S, Grafi G, Ohad N. Interaction of FIE, a polycomb protein, with pRb: a possible mechanism regulating endosperm development. Mol Genet Genomics. 2004;271:651-7 pubmed
    Inactivation of the Arabidopsis protein FERTILIZATION INDEPENDENT ENDOSPERM (FIE) induces division of the central cell of the embryo sac, leading to endosperm development in the absence of fertilization...
  4. Bouyer D, Roudier F, Heese M, Andersen E, Gey D, Nowack M, et al. Polycomb repressive complex 2 controls the embryo-to-seedling phase transition. PLoS Genet. 2011;7:e1002014 pubmed publisher
    ..the requirement of PRC2 in endosperm allowed us to generate viable homozygous null mutants for FERTILIZATION INDEPENDENT ENDOSPERM (FIE), which is the single Arabidopsis homolog of Extra Sex Combs, an indispensable component of ..
  5. Liang S, Hartwig B, Perera P, Mora García S, de Leau E, Thornton H, et al. Kicking against the PRCs - A Domesticated Transposase Antagonises Silencing Mediated by Polycomb Group Proteins and Is an Accessory Component of Polycomb Repressive Complex 2. PLoS Genet. 2015;11:e1005660 pubmed publisher
  6. Schmidt A, Wöhrmann H, Raissig M, Arand J, Gheyselinck J, Gagliardini V, et al. The Polycomb group protein MEDEA and the DNA methyltransferase MET1 interact to repress autonomous endosperm development in Arabidopsis. Plant J. 2013;73:776-87 pubmed publisher
    ..The FERTILIZATION-INDEPENDENT SEED (FIS) class genes MEDEA (MEA) and FERTILIZATION-INDEPENDENT ENDOSPERM (FIE) encode two of the core components of this complex...
  7. De Lucia F, Crevillen P, Jones A, Greb T, Dean C. A PHD-polycomb repressive complex 2 triggers the epigenetic silencing of FLC during vernalization. Proc Natl Acad Sci U S A. 2008;105:16831-6 pubmed publisher
    ..prolonged cold a PHD-PRC2 complex forms composed of core PRC2 components (VRN2, SWINGER [an E(Z) HMTase homologue], FIE [an ESC homologue], MSI1 [p55 homologue]), and three related PHD finger proteins, VRN5, VIN3, and VEL1...
  8. Rodrigues J, Tucker M, Johnson S, Hrmova M, Koltunow A. Sexual and apomictic seed formation in Hieracium requires the plant polycomb-group gene FERTILIZATION INDEPENDENT ENDOSPERM. Plant Cell. 2008;20:2372-86 pubmed publisher
    ..To investigate FIS function during apomictic seed formation, FERTILIZATION INDEPENDENT ENDOSPERM (FIE), encoding a WD-repeat member of the FIS complex, was isolated and downregulated in sexual and ..
  9. Deng W, Buzas D, Ying H, Robertson M, Taylor J, Peacock W, et al. Arabidopsis Polycomb Repressive Complex 2 binding sites contain putative GAGA factor binding motifs within coding regions of genes. BMC Genomics. 2013;14:593 pubmed publisher
    ..ChIP-seq to determine the genome-wide distribution of hemagglutinin (HA)-tagged FERTLIZATION INDEPENDENT ENDOSPERM (FIE-HA), the Extra Sex Combs homolog protein present in all Arabidopsis PRC2 complexes...
  10. Jullien P, Katz A, Oliva M, Ohad N, Berger F. Polycomb group complexes self-regulate imprinting of the Polycomb group gene MEDEA in Arabidopsis. Curr Biol. 2006;16:486-92 pubmed
    ..This feedback loop ensures a complete maternal control of MEA expression from both parental alleles and might have provided a template for evolution of imprinting in plants. ..