Gene Symbol: DREB1A
Description: dehydration response element B1A
Alias: ATCBF3, C-REPEAT BINDING FACTOR 3, C-REPEAT BINDING FACTOR 3, CBF3, T30C3.3, TRANSCRIPTIONAL ACTIVATOR CBF3, dehydration response element B1A, dehydration response element B1A
Species: thale cress

Top Publications

  1. Rayirath P, Benkel B, Mark Hodges D, Allan Wojtas P, MacKinnon S, Critchley A, et al. Lipophilic components of the brown seaweed, Ascophyllum nodosum, enhance freezing tolerance in Arabidopsis thaliana. Planta. 2009;230:135-47 pubmed publisher
    ..Further, the A. nodosum extract treatment modulated the expression of the cold response genes, COR15A, RD29A, and CBF3, resulting in enhanced tolerance to freezing temperatures. More than 2.6-fold increase in expression of RD29A, 1...
  2. Fowler S, Cook D, Thomashow M. Low temperature induction of Arabidopsis CBF1, 2, and 3 is gated by the circadian clock. Plant Physiol. 2005;137:961-8 pubmed
    ..Recently, it has been shown that basal levels of CBF3 transcripts and those of certain CBF-regulated genes exhibit circadian cycling...
  3. Sakuma Y, Maruyama K, Osakabe Y, Qin F, Seki M, Shinozaki K, et al. Functional analysis of an Arabidopsis transcription factor, DREB2A, involved in drought-responsive gene expression. Plant Cell. 2006;18:1292-309 pubmed
    Transcription factors DREB1A/CBF3 and DREB2A specifically interact with cis-acting dehydration-responsive element/C-repeat (DRE/CRT) involved in cold and drought stress-responsive gene expression in Arabidopsis thaliana...
  4. Novillo F, Medina J, Salinas J. Arabidopsis CBF1 and CBF3 have a different function than CBF2 in cold acclimation and define different gene classes in the CBF regulon. Proc Natl Acad Sci U S A. 2007;104:21002-7 pubmed
    ..proteins constitute a small family of Arabidopsis transcriptional activators (CBF1/DREB1B, CBF2/DREB1C, and CBF3/DREB1A) that play a prominent role in cold acclimation...
  5. Polizel A, Medri M, Nakashima K, Yamanaka N, Farias J, de Oliveira M, et al. Molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance. Genet Mol Res. 2011;10:3641-56 pubmed publisher
    ..This construct expressed dehydration- responsive element binding protein DREB1A from the stress-inducible rd29A promoter...
  6. Oh S, Song S, Kim Y, Jang H, Kim S, Kim M, et al. Arabidopsis CBF3/DREB1A and ABF3 in transgenic rice increased tolerance to abiotic stress without stunting growth. Plant Physiol. 2005;138:341-51 pubmed
    ..in comparison with that of Arabidopsis, we developed transgenic rice plants that constitutively expressed CBF3/DREB1A (CBF3) and ABF3, Arabidopsis genes that function in abscisic acid-independent and abscisic acid-dependent stress-..
  7. Anbazhagan K, Bhatnagar Mathur P, Vadez V, Dumbala S, Kishor P, Sharma K. DREB1A overexpression in transgenic chickpea alters key traits influencing plant water budget across water regimes. Plant Cell Rep. 2015;34:199-210 pubmed publisher
    We demonstrate the role of DREB1A transcription factor in better root and shoot partitioning and higher transpiration efficiency in transgenic chickpea under drought stress Chickpea (Cicer arietinum L...
  8. Seo E, Lee H, Jeon J, Park H, Kim J, Noh Y, et al. Crosstalk between cold response and flowering in Arabidopsis is mediated through the flowering-time gene SOC1 and its upstream negative regulator FLC. Plant Cell. 2009;21:3185-97 pubmed publisher
  9. Kang J, Zhang H, Sun T, Shi Y, Wang J, Zhang B, et al. Natural variation of C-repeat-binding factor (CBFs) genes is a major cause of divergence in freezing tolerance among a group of Arabidopsis thaliana populations along the Yangtze River in China. New Phytol. 2013;199:1069-80 pubmed publisher
    ..The CBF2 in SXcgx experienced a stronger natural selection in the cooler environment after CBF3 lost its response to low temperature, which possibly reflects a local adaptation of these populations during the ..

More Information


  1. Gehan M, Park S, Gilmour S, An C, Lee C, Thomashow M. Natural variation in the C-repeat binding factor cold response pathway correlates with local adaptation of Arabidopsis ecotypes. Plant J. 2015;84:682-93 pubmed publisher
    ..The CBF locus includes three genes - CBF1, CBF2 and CBF3 - that are induced by low temperature and encode transcription factors that regulate a group of more than 100 genes,..
  2. Shinwari Z, Nakashima K, Miura S, Kasuga M, Seki M, Yamaguchi Shinozaki K, et al. An Arabidopsis gene family encoding DRE/CRT binding proteins involved in low-temperature-responsive gene expression. Biochem Biophys Res Commun. 1998;250:161-70 pubmed
    ..from the cold stress signal to gene expression, we characterized a gene family for DRE/CRT-binding proteins DREB1A and CBF1 in Arabidopsis thaliana...
  3. Miura K, Jin J, Lee J, Yoo C, Stirm V, Miura T, et al. SIZ1-mediated sumoylation of ICE1 controls CBF3/DREB1A expression and freezing tolerance in Arabidopsis. Plant Cell. 2007;19:1403-14 pubmed
    ..Cold-induced expression of CBF/DREB1, particularly of CBF3/DREB1A, and of the regulon genes was repressed by siz1...
  4. Chan Z, Wang Y, Cao M, Gong Y, Mu Z, Wang H, et al. RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway. New Phytol. 2016;209:1527-39 pubmed publisher
    ..immunoprecipitation results suggested that RDM4 is important for Pol II occupancy at the promoters of CBF2 and CBF3. We present evidence of a considerable role for RDM4 in regulating gene expression at low temperature, including ..
  5. Akhtar M, Jaiswal A, Taj G, Jaiswal J, Qureshi M, Singh N. DREB1/CBF transcription factors: their structure, function and role in abiotic stress tolerance in plants. J Genet. 2012;91:385-95 pubmed
    ..The recent progress related to genetic engineering of DREB1/CBF transcription factors in various crops and model plants is also summarized. ..
  6. Seki M, Narusaka M, Ishida J, Nanjo T, Fujita M, Oono Y, et al. Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. Plant J. 2002;31:279-92 pubmed
    ..The cold-inducible genes were classified into at least two gene groups from their expression profiles. DREB1A was included in a group whose expression peaked at 2 h after cold treatment...
  7. Behnam B, Kikuchi A, Celebi Toprak F, Kasuga M, Yamaguchi Shinozaki K, Watanabe K. Arabidopsis rd29A::DREB1A enhances freezing tolerance in transgenic potato. Plant Cell Rep. 2007;26:1275-82 pubmed
    ..The lines were transgenic for the DREB1A gene under control of the rd29A promoter, both of which were derived from Arabidopsis thaliana...
  8. Shi Y, Huang J, Sun T, Wang X, Zhu C, Ai Y, et al. The precise regulation of different COR genes by individual CBF transcription factors in Arabidopsis thaliana. J Integr Plant Biol. 2017;59:118-133 pubmed publisher
    ..The C-repeat (CRT) motif (5'-CCGAC-3') was enriched in promoters of genes that were up-regulated by CBF2 and CBF3 but not in promoters of genes up-regulated by CBF1...
  9. Maruyama K, Takeda M, Kidokoro S, Yamada K, Sakuma Y, Urano K, et al. Metabolic pathways involved in cold acclimation identified by integrated analysis of metabolites and transcripts regulated by DREB1A and DREB2A. Plant Physiol. 2009;150:1972-80 pubmed publisher
    b>DREB1A/CBF3 and DREB2A are transcription factors that specifically interact with a cis-acting dehydration-responsive element (DRE), which is involved in cold- and dehydration-responsive gene expression in Arabidopsis (Arabidopsis thaliana)..
  10. Oraby H, Ahmad R. Physiological and biochemical changes of CBF3 transgenic oat in response to salinity stress. Plant Sci. 2012;185-186:331-9 pubmed publisher
    ..The impact of introducing the Arabidopsis CBF3 gene controlled by the rd29A stress-inducible promoter in T(2) transgenic oat on salinity tolerance and associated ..
  11. Vergnolle C, Vaultier M, Taconnat L, Renou J, Kader J, Zachowski A, et al. The cold-induced early activation of phospholipase C and D pathways determines the response of two distinct clusters of genes in Arabidopsis cell suspensions. Plant Physiol. 2005;139:1217-33 pubmed
    ..The connection of these pathways with the CBF pathway, currently the most understood genetic system playing a role in cold acclimation, is discussed. ..
  12. Suo H, Ma Q, Ye K, Yang C, Tang Y, Hao J, et al. Overexpression of AtDREB1A causes a severe dwarf phenotype by decreasing endogenous gibberellin levels in soybean [Glycine max (L.) Merr]. PLoS ONE. 2012;7:e45568 pubmed publisher
    ..In this study, the Arabidopsis DREB1A gene driven by the CaMV 35S promoter was introduced into soybean plants by Agrobacterium- mediated transformation...
  13. Dou H, Xv K, Meng Q, Li G, Yang X. Potato plants ectopically expressing Arabidopsis thaliana?CBF3 exhibit enhanced tolerance to high-temperature stress. Plant Cell Environ. 2015;38:61-72 pubmed publisher
    ..Our results revealed that temperature of 40?°C or higher can significantly induce AtCBF3 expression...
  14. Ravikumar G, Manimaran P, Voleti S, Subrahmanyam D, Sundaram R, Bansal K, et al. Stress-inducible expression of AtDREB1A transcription factor greatly improves drought stress tolerance in transgenic indica rice. Transgenic Res. 2014;23:421-39 pubmed publisher
    ..The transcription factor DREB1A, identified from the model plant Arabidopsis thaliana, has been reported to enhance stress tolerance against ..
  15. Liu Z, Jia Y, Ding Y, Shi Y, Li Z, Guo Y, et al. Plasma Membrane CRPK1-Mediated Phosphorylation of 14-3-3 Proteins Induces Their Nuclear Import to Fine-Tune CBF Signaling during Cold Response. Mol Cell. 2017;66:117-128.e5 pubmed publisher
    ..Thus, our study reveals that the CRPK1-14-3-3 module transduces the cold signal from the plasma membrane to the nucleus to modulate CBF stability, which ensures a faithfully adjusted response to cold stress of plants. ..
  16. Jia Y, Ding Y, Shi Y, Zhang X, Gong Z, Yang S. The cbfs triple mutants reveal the essential functions of CBFs in cold acclimation and allow the definition of CBF regulons in Arabidopsis. New Phytol. 2016;212:345-53 pubmed publisher
    ..In this study, we used CRISPR/Cas9 technology to mutate CBF1 or CBF1/CBF2 in a cbf3 T-DNA insertion mutant to generate cbf1,3 double and cbf1 cbf2 cbf3 (cbfs) triple mutants...
  17. Lee S, Kang J, Park H, Kim M, Bae M, Choi H, et al. DREB2C interacts with ABF2, a bZIP protein regulating abscisic acid-responsive gene expression, and its overexpression affects abscisic acid sensitivity. Plant Physiol. 2010;153:716-27 pubmed publisher
    ..We further show that other AP2 domain proteins, DREB1A and DREB2A, interact with ABF2 and that other ABF family members, ABF3 and ABF4, interact with DREB2C...
  18. Kim Y, Lee M, Lee J, Lee H, Park C. The unified ICE-CBF pathway provides a transcriptional feedback control of freezing tolerance during cold acclimation in Arabidopsis. Plant Mol Biol. 2015;89:187-201 pubmed publisher
    ..Inducer of CBF expression 1 (ICE1) plays a key role by activating CBF3 expression in shaping the cold-induced transcriptome...
  19. Wei T, Deng K, Liu D, Gao Y, Liu Y, Yang M, et al. Ectopic Expression of DREB Transcription Factor, AtDREB1A, Confers Tolerance to Drought in Transgenic Salvia miltiorrhiza. Plant Cell Physiol. 2016;57:1593-609 pubmed publisher
    ..The Arabidopsis thaliana gene DREB1A/CBF3, encoding a stress-inducible TF, was introduced into Salvia miltiorrhiza Ectopic expression of AtDREB1A ..
  20. Wan F, Pan Y, Li J, Chen X, Pan Y, Wang Y, et al. Heterologous expression of Arabidopsis C-repeat binding factor 3 (AtCBF3) and cold-regulated 15A (AtCOR15A) enhanced chilling tolerance in transgenic eggplant (Solanum melongena L.). Plant Cell Rep. 2014;33:1951-61 pubmed publisher
    Our study shows that the expression of AtCBF3 and AtCOR15A improved the chilling tolerance in transgenic eggplant...
  21. Chen L, Lee J, Weber H, Tohge T, Witt S, Roje S, et al. Arabidopsis BPM proteins function as substrate adaptors to a cullin3-based E3 ligase to affect fatty acid metabolism in plants. Plant Cell. 2013;25:2253-64 pubmed publisher
    ..Overall, this work demonstrates a link between fatty acid metabolism and E3 ligase activities in plants and establishes CUL3-based E3 ligases as key regulators in transcriptional processes that involve ERF/AP2 family members. ..
  22. Lin Y, Hwang S, Hsu P, Chiang Y, Huang C, Wang C, et al. Molecular population genetics and gene expression analysis of duplicated CBF genes of Arabidopsis thaliana. BMC Plant Biol. 2008;8:111 pubmed publisher
    ..With respect to the level of CBF gene expression, several mutated nucleotides in the promoters of CBF3 and -1 of specific ecotypes might be responsible for its consistently low expression...
  23. Seki M, Narusaka M, Abe H, Kasuga M, Yamaguchi Shinozaki K, Carninci P, et al. Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray. Plant Cell. 2001;13:61-72 pubmed
    ..approximately 1300 full-length Arabidopsis cDNAs to identify drought- and cold-inducible genes and target genes of DREB1A/CBF3, a transcription factor that controls stress-inducible gene expression...
  24. Kasuga M, Liu Q, Miura S, Yamaguchi Shinozaki K, Shinozaki K. Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nat Biotechnol. 1999;17:287-91 pubmed
    ..The transcription factor DREB1A specifically interacts with the DRE and induces expression of stress tolerance genes...
  25. Hu Y, Jiang L, Wang F, Yu D. Jasmonate regulates the inducer of cbf expression-C-repeat binding factor/DRE binding factor1 cascade and freezing tolerance in Arabidopsis. Plant Cell. 2013;25:2907-24 pubmed publisher
    ..Taken together, our study provides evidence that jasmonate functions as a critical upstream signal of the ICE-CBF/DREB1 pathway to positively regulate Arabidopsis freezing tolerance...
  26. Zhao C, Zhang Z, Xie S, Si T, Li Y, Zhu J. Mutational Evidence for the Critical Role of CBF Transcription Factors in Cold Acclimation in Arabidopsis. Plant Physiol. 2016;171:2744-59 pubmed publisher
    The three tandemly arranged CBF genes, CBF1, CBF2, and CBF3, are involved in cold acclimation...
  27. Maruyama K, Sakuma Y, Kasuga M, Ito Y, Seki M, Goda H, et al. Identification of cold-inducible downstream genes of the Arabidopsis DREB1A/CBF3 transcriptional factor using two microarray systems. Plant J. 2004;38:982-93 pubmed
    ..Transgenic plants overexpressing DREB1A showed activated expression of many stress-inducible genes and improved tolerance to not only drought, salinity, ..