DRB4

Summary

Gene Symbol: DRB4
Description: double-stranded-RNA-binding protein 4
Alias: ATTIF3K1, double-stranded-RNA-binding protein 4, double-stranded-RNA-binding protein 4
Species: thale cress

Top Publications

  1. Hiraguri A, Itoh R, Kondo N, Nomura Y, Aizawa D, Murai Y, et al. Specific interactions between Dicer-like proteins and HYL1/DRB-family dsRNA-binding proteins in Arabidopsis thaliana. Plant Mol Biol. 2005;57:173-88 pubmed
    ..one or two dsRBMs: four Arabidopsis Dicer-like proteins (DCL1-4), Arabidopsis HYL1 and four of its homologs (DRB2, DRB4, DRB5 and OsDRB1)...
  2. Adenot X, Elmayan T, Lauressergues D, Boutet S, Bouche N, Gasciolli V, et al. DRB4-dependent TAS3 trans-acting siRNAs control leaf morphology through AGO7. Curr Biol. 2006;16:927-32 pubmed
    ..Consistently, tas3 but not tas1 or tas2 mutants exhibits leaf morphology defects, and ago7/zip and drb4 mutants, which exhibit leaf morphology defects, lack TAS3 but not TAS1 and TAS2 ta-siRNAs in leaves...
  3. Nakazawa Y, Hiraguri A, Moriyama H, Fukuhara T. The dsRNA-binding protein DRB4 interacts with the Dicer-like protein DCL4 in vivo and functions in the trans-acting siRNA pathway. Plant Mol Biol. 2007;63:777-85 pubmed
    ..We have previously demonstrated that DCL4 specifically interacts with DRB4 in vitro. Here we describe the interaction between DCL4 and DRB4 in vivo...
  4. Fukudome A, Kanaya A, Egami M, Nakazawa Y, Hiraguri A, Moriyama H, et al. Specific requirement of DRB4, a dsRNA-binding protein, for the in vitro dsRNA-cleaving activity of Arabidopsis Dicer-like 4. RNA. 2011;17:750-60 pubmed publisher
    ..small interfering RNAs from both endogenous and exogenous double-stranded RNAs (dsRNAs), and it interacts with DRB4, a dsRNA-binding protein, in vivo and in vitro...
  5. Mohannath G, Jackel J, Lee Y, Buchmann R, Wang H, Patil V, et al. A complex containing SNF1-related kinase (SnRK1) and adenosine kinase in Arabidopsis. PLoS ONE. 2014;9:e87592 pubmed publisher
    ..This study establishes the existence of SnRK1-ADK complexes that may play important roles in energy homeostasis and cellular responses to biotic and abiotic stress. ..
  6. Zhu S, Lim G, Yu K, Jeong R, Kachroo A, Kachroo P. RNA silencing components mediate resistance signaling against turnip crinkle virus. Plant Signal Behav. 2014;9:e28435 pubmed
    ..Intriguingly, TCV infection promotes the cytosolic accumulation of the otherwise primarily nuclear DRB4, and this in turn inhibits HRT-DRB4 interaction...
  7. Haas G, Azevedo J, Moissiard G, Geldreich A, Himber C, Bureau M, et al. Nuclear import of CaMV P6 is required for infection and suppression of the RNA silencing factor DRB4. EMBO J. 2008;27:2102-12 pubmed publisher
    ..Transgenic P6 expression in Arabidopsis is genetically equivalent to inactivating the nuclear protein DRB4 that facilitates the activity of the major plant antiviral silencing factor DCL4...
  8. Qu F, Ye X, Morris T. Arabidopsis DRB4, AGO1, AGO7, and RDR6 participate in a DCL4-initiated antiviral RNA silencing pathway negatively regulated by DCL1. Proc Natl Acad Sci U S A. 2008;105:14732-7 pubmed publisher
    ..We also implicate DRB4 in antiviral RNA silencing...
  9. Qin H, Chen F, Huan X, Machida S, Song J, Yuan Y. Structure of the Arabidopsis thaliana DCL4 DUF283 domain reveals a noncanonical double-stranded RNA-binding fold for protein-protein interaction. RNA. 2010;16:474-81 pubmed publisher
    ..weak dsRNA binding affinity and specifically binds to double-stranded RNA-binding domain 1 (dsRBD1) of Arabidopsis DRB4, whereas the DUF283 domain of DCL1 specifically binds to dsRBD2 of Arabidopsis HYL1...

More Information

Publications14

  1. PĂ©lissier T, Clavel M, Chaparro C, Pouch PĂ©lissier M, Vaucheret H, Deragon J. Double-stranded RNA binding proteins DRB2 and DRB4 have an antagonistic impact on polymerase IV-dependent siRNA levels in Arabidopsis. RNA. 2011;17:1502-10 pubmed publisher
    ..Here we show that the double-stranded RNA binding proteins DRB2 and DRB4 are required for proper accumulation of p4-siRNAs...
  2. Jakubiec A, Yang S, Chua N. Arabidopsis DRB4 protein in antiviral defense against Turnip yellow mosaic virus infection. Plant J. 2012;69:14-25 pubmed publisher
    ..Here, we showed that DCL4 and its interacting partner dsRNA-binding protein 4 (DRB4) participate in the antiviral response to Turnip yellow mosaic virus (TYMV), and that both proteins are required ..
  3. Marrocco K, Criqui M, Zervudacki J, Schott G, Eisler H, Parnet A, et al. APC/C-mediated degradation of dsRNA-binding protein 4 (DRB4) involved in RNA silencing. PLoS ONE. 2012;7:e35173 pubmed publisher
    ..This screen identified DRB4, a double-stranded RNA binding protein involved in the biogenesis of different classes of small RNA (sRNA)...
  4. Tschopp M, Iki T, Brosnan C, Jullien P, Pumplin N. A complex of Arabidopsis DRB proteins can impair dsRNA processing. RNA. 2017;23:782-797 pubmed publisher
    ..To gain insight into this pathway in the model plant Arabidopsis, we searched for interactors of DRB4 by immunoprecipitation followed by mass spectrometry-based fingerprinting and discovered DRB7.1...
  5. Chiliveri S, Aute R, Rai U, Deshmukh M. DRB4 dsRBD1 drives dsRNA recognition in Arabidopsis thaliana tasi/siRNA pathway. Nucleic Acids Res. 2017;45:8551-8563 pubmed publisher
    In Arabidopsis thaliana, endogenous trans-acting and exogenous siRNA pathways are initiated by the interaction of DRB4 with trigger dsRNA. Further, DCL4:DRB4 complex cleaves the dsRNA into 21 bp siRNA...