DML1

Summary

Gene Symbol: DML1
Description: demeter-like 1
Alias: AtROS1, F1O11.12, F1O11_12, REPRESSOR OF SILENCING1, ROS1, demeter-like 1, demeter-like 1
Species: thale cress

Top Publications

  1. Gong Z, Morales Ruiz T, Ariza R, Roldan Arjona T, David L, Zhu J. ROS1, a repressor of transcriptional gene silencing in Arabidopsis, encodes a DNA glycosylase/lyase. Cell. 2002;111:803-14 pubmed
    Mutations in the Arabidopsis ROS1 locus cause transcriptional silencing of a transgene and a homologous endogenous gene...
  2. Ponferrada Marín M, Roldan Arjona T, Ariza R. ROS1 5-methylcytosine DNA glycosylase is a slow-turnover catalyst that initiates DNA demethylation in a distributive fashion. Nucleic Acids Res. 2009;37:4264-74 pubmed publisher
    Arabidopsis ROS1 belongs to a family of plant 5-methycytosine DNA glycosylases that initiate DNA demethylation through base excision...
  3. Martínez Macías M, Córdoba Cañero D, Ariza R, Roldan Arjona T. The DNA repair protein XRCC1 functions in the plant DNA demethylation pathway by stimulating cytosine methylation (5-meC) excision, gap tailoring, and DNA ligation. J Biol Chem. 2013;288:5496-505 pubmed publisher
    ..Active DNA demethylation in plants is mediated by a family of DNA glycosylases typified by Arabidopsis ROS1 (repressor of silencing 1)...
  4. Agius F, Kapoor A, Zhu J. Role of the Arabidopsis DNA glycosylase/lyase ROS1 in active DNA demethylation. Proc Natl Acad Sci U S A. 2006;103:11796-801 pubmed
    ..Genetic evidence suggested that the DNA glycosylase domain-containing protein ROS1 of Arabidopsis is a putative DNA demethylase, because loss-of-function ros1 mutations cause DNA hypermethylation ..
  5. Zhu J, Kapoor A, Sridhar V, Agius F, Zhu J. The DNA glycosylase/lyase ROS1 functions in pruning DNA methylation patterns in Arabidopsis. Curr Biol. 2007;17:54-9 pubmed
    The Arabidopsis DNA glycosylase/lyase ROS1 participates in active DNA demethylation by a base-excision pathway. ROS1 has been shown to be required for demethylating a transgene promoter...
  6. Morales Ruiz T, Ortega Galisteo A, Ponferrada Marín M, Martínez Macías M, Ariza R, Roldan Arjona T. DEMETER and REPRESSOR OF SILENCING 1 encode 5-methylcytosine DNA glycosylases. Proc Natl Acad Sci U S A. 2006;103:6853-8 pubmed
    ..activates the maternal expression of two imprinted genes silenced by methylation, and REPRESSOR OF SILENCING 1 (ROS1) is required for release of transcriptional silencing of a hypermethylated transgene...
  7. Martínez Macías M, Qian W, Miki D, Pontes O, Liu Y, Tang K, et al. A DNA 3' phosphatase functions in active DNA demethylation in Arabidopsis. Mol Cell. 2012;45:357-70 pubmed publisher
    ..After 5-methylcytosine removal, the Arabidopsis DNA glycosylase/lyase ROS1 incises the DNA backbone and part of the product has a single-nucleotide gap flanked by 3'- and 5'-phosphate ..
  8. Xia R, Wang J, Liu C, Wang Y, Wang Y, Zhai J, et al. ROR1/RPA2A, a putative replication protein A2, functions in epigenetic gene silencing and in regulation of meristem development in Arabidopsis. Plant Cell. 2006;18:85-103 pubmed
    We screened for suppressors of repressor of silencing1 (ros1) using the silenced 35S promoter-neomycin phosphotransferase II (Pro(35S):NPTII) gene as a marker and identified two allelic mutants, ror1-1 and ror1-2 (for suppressor of ros1)...
  9. Li Y, Córdoba Cañero D, Qian W, Zhu X, Tang K, Zhang H, et al. An AP endonuclease functions in active DNA demethylation and gene imprinting in Arabidopsis [corrected]. PLoS Genet. 2015;11:e1004905 pubmed publisher
    ..demethylation in plants occurs through base excision repair, beginning with removal of methylated cytosine by the ROS1/DME subfamily of 5-methylcytosine DNA glycosylases...

More Information

Publications25

  1. Yamamuro C, Miki D, Zheng Z, Ma J, Wang J, Yang Z, et al. Overproduction of stomatal lineage cells in Arabidopsis mutants defective in active DNA demethylation. Nat Commun. 2014;5:4062 pubmed publisher
    ..In Arabidopsis, active DNA demethylation depends on the function of the ROS1 subfamily of genes that encode 5-methylcytosine DNA glycosylases/lyases...
  2. Kapoor A, Agius F, Zhu J. Preventing transcriptional gene silencing by active DNA demethylation. FEBS Lett. 2005;579:5889-98 pubmed
    ..Mutations in the bifunctional DNA glycosylase/lyase ROS1 in Arabidopsis cause DNA hypermethylation and transcriptional silencing of specific genes...
  3. López Sánchez A, Stassen J, Furci L, Smith L, Ton J. The role of DNA (de)methylation in immune responsiveness of Arabidopsis. Plant J. 2016;88:361-374 pubmed publisher
    ..of the hypo-methylated nrpe1 mutant, which is impaired in RNA-directed DNA methylation, and the hyper-methylated ros1 mutant, which is affected in DNA demethylation, revealed that their opposite resistance phenotypes are associated ..
  4. Liu J, Ren X, Yin H, Wang Y, Xia R, Wang Y, et al. Mutation in the catalytic subunit of DNA polymerase alpha influences transcriptional gene silencing and homologous recombination in Arabidopsis. Plant J. 2010;61:36-45 pubmed publisher
    REPRESSOR OF SILENCING 1 (ROS1) encodes a DNA demethylase that actively removes DNA methylation...
  5. Han Y, Dou K, Ma Z, Zhang S, Huang H, Li L, et al. SUVR2 is involved in transcriptional gene silencing by associating with SNF2-related chromatin-remodeling proteins in Arabidopsis. Cell Res. 2014;24:1445-65 pubmed publisher
    ..Our study suggests that the involvement of SUVR2 in transcriptional gene silencing is related to nucleosome positioning mediated by its associated chromatin-remodeling proteins. ..
  6. Zhao Y, Xie S, Li X, Wang C, Chen Z, Lai J, et al. REPRESSOR OF SILENCING5 Encodes a Member of the Small Heat Shock Protein Family and Is Required for DNA Demethylation in Arabidopsis. Plant Cell. 2014;26:2660-2675 pubmed
    In Arabidopsis thaliana, active DNA demethylation is initiated by the DNA glycosylase REPRESSOR OF SILENCING1 (ROS1) and its paralogs DEMETER, DEMETER-LIKE2 (DML2), and DML3...
  7. Williams B, Pignatta D, Henikoff S, Gehring M. Methylation-sensitive expression of a DNA demethylase gene serves as an epigenetic rheostat. PLoS Genet. 2015;11:e1005142 pubmed publisher
    ..we show that both RdDM and DNA demethylation are highly active at a TE proximal to the major DNA demethylase gene ROS1. Unexpectedly, and in contrast to most other genomic targets, expression of ROS1 is promoted by DNA methylation and ..
  8. Penterman J, Uzawa R, Fischer R. Genetic interactions between DNA demethylation and methylation in Arabidopsis. Plant Physiol. 2007;145:1549-57 pubmed
    ..Three DEMETER-LIKE (DML) proteins, REPRESSOR OF SILENCING1 (ROS1), DML2, and DML3, function to protect genes from potentially deleterious methylation...
  9. Duan C, Wang X, Tang K, Zhang H, Mangrauthia S, Lei M, et al. MET18 Connects the Cytosolic Iron-Sulfur Cluster Assembly Pathway to Active DNA Demethylation in Arabidopsis. PLoS Genet. 2015;11:e1005559 pubmed publisher
    DNA demethylation mediated by the DNA glycosylase ROS1 helps determine genomic DNA methylation patterns and protects active genes from being silenced. However, little is known about the mechanism of regulation of ROS1 enzymatic activity...
  10. Wang X, Li Q, Yuan W, Cao Z, Qi B, Kumar S, et al. The cytosolic Fe-S cluster assembly component MET18 is required for the full enzymatic activity of ROS1 in active DNA demethylation. Sci Rep. 2016;6:26443 pubmed publisher
    ..Beginning with the removal of methylated cytosine by ROS1/DME family of 5-methylcytosine DNA glycosylases, active DNA demethylation in plants occurs through base excision ..
  11. Zhou H, Zhang F, Ma Z, Huang H, Jiang L, Cai T, et al. Folate polyglutamylation is involved in chromatin silencing by maintaining global DNA methylation and histone H3K9 dimethylation in Arabidopsis. Plant Cell. 2013;25:2545-59 pubmed publisher
    ..identify factors required for DNA methylation and H3K9 dimethylation, we screened for suppressors of the repressor of silencing1 (ros1) mutation, which causes silencing of the expression of the RD29A (RESPONSE TO DESSICATION 29A) ..
  12. Bharti P, Mahajan M, Vishwakarma A, Bhardwaj J, Yadav S. AtROS1 overexpression provides evidence for epigenetic regulation of genes encoding enzymes of flavonoid biosynthesis and antioxidant pathways during salt stress in transgenic tobacco. J Exp Bot. 2015;66:5959-69 pubmed publisher
    ..For this, a repressor of silencing from Arabidopsis, AtROS1, was overexpressed in transgenic tobacco...
  13. Tang K, Lang Z, Zhang H, Zhu J. The DNA demethylase ROS1 targets genomic regions with distinct chromatin modifications. Nat Plants. 2016;2:16169 pubmed publisher
    The Arabidopsis ROS1/DEMETER family of 5-methylcytosine (5mC) DNA glycosylases are the first genetically characterized DNA demethylases in eukaryotes. However, the features of ROS1-targeted genomic loci are not well understood...
  14. Li X, Qian W, Zhao Y, Wang C, Shen J, Zhu J, et al. Antisilencing role of the RNA-directed DNA methylation pathway and a histone acetyltransferase in Arabidopsis. Proc Natl Acad Sci U S A. 2012;109:11425-30 pubmed publisher
    REPRESSOR OF SILENCING 1 (ROS1) is a DNA demethylation enzyme that was previously identified during a genetic screen for the silencing of both RD29A-LUC and 35S-NPTII transgenes on a T-DNA construct...
  15. Le T, Schumann U, Smith N, Tiwari S, Au P, Zhu Q, et al. DNA demethylases target promoter transposable elements to positively regulate stress responsive genes in Arabidopsis. Genome Biol. 2014;15:458 pubmed publisher
    ..Arabidopsis encodes four DNA demethylases, DEMETER (DME), REPRESSOR OF SILENCING 1 (ROS1), DEMETER-LIKE 2 (DML2), and DML3...
  16. Kapoor A, Agarwal M, Andreucci A, Zheng X, Gong Z, Hasegawa P, et al. Mutations in a conserved replication protein suppress transcriptional gene silencing in a DNA-methylation-independent manner in Arabidopsis. Curr Biol. 2005;15:1912-8 pubmed
    Mutations in the DNA glycosylase/lyase ROS1 cause transcriptional silencing of the linked RD29A-LUC and 35S-NPTII transgenes in Arabidopsis...