Gene Symbol: DCL4
Description: dicer-like 4
Alias: ATDCL4, DICER-LIKE 4, F5O24.210, F5O24_210, dicer-like 4, dicer-like 4
Species: thale cress

Top Publications

  1. Hiraguri A, Itoh R, Kondo N, Nomura Y, Aizawa D, Murai Y, et al. Specific interactions between Dicer-like proteins and HYL1/DRB-family dsRNA-binding proteins in Arabidopsis thaliana. Plant Mol Biol. 2005;57:173-88 pubmed
    ..We show that DRB4 interacts specifically with DCL4, and HYL1 most strongly interacts with DCL1...
  2. Du Q, Duan C, Zhang Z, Fang Y, Fang R, Xie Q, et al. DCL4 targets Cucumber mosaic virus satellite RNA at novel secondary structures. J Virol. 2007;81:9142-51 pubmed
    ..hierarchical actions of plant Dicer-like (DCL) proteins are required in the biogenesis process of small RNAs, and DCL4 is the primary producer of vsiRNAs...
  3. Gasciolli V, Mallory A, Bartel D, Vaucheret H. Partially redundant functions of Arabidopsis DICER-like enzymes and a role for DCL4 in producing trans-acting siRNAs. Curr Biol. 2005;15:1494-500 pubmed
    ..DCL2 produces some virus-derived siRNAs, and DCL3 produces endogenous RDR2-dependent siRNAs, but the role of DCL4 is unknown. We show that DCL4 is the primary processor of endogenous RDR6-dependent trans-acting siRNAs (tasiRNAs)...
  4. Nakazawa Y, Hiraguri A, Moriyama H, Fukuhara T. The dsRNA-binding protein DRB4 interacts with the Dicer-like protein DCL4 in vivo and functions in the trans-acting siRNA pathway. Plant Mol Biol. 2007;63:777-85 pubmed
    ..We have previously demonstrated that DCL4 specifically interacts with DRB4 in vitro. Here we describe the interaction between DCL4 and DRB4 in vivo...
  5. Fukudome A, Kanaya A, Egami M, Nakazawa Y, Hiraguri A, Moriyama H, et al. Specific requirement of DRB4, a dsRNA-binding protein, for the in vitro dsRNA-cleaving activity of Arabidopsis Dicer-like 4. RNA. 2011;17:750-60 pubmed publisher
    Arabidopsis thaliana Dicer-like 4 (DCL4) produces 21-nt small interfering RNAs from both endogenous and exogenous double-stranded RNAs (dsRNAs), and it interacts with DRB4, a dsRNA-binding protein, in vivo and in vitro...
  6. Blevins T, Rajeswaran R, Shivaprasad P, Beknazariants D, Si Ammour A, Park H, et al. Four plant Dicers mediate viral small RNA biogenesis and DNA virus induced silencing. Nucleic Acids Res. 2006;34:6233-46 pubmed
    ..In contrast, RNA virus infection is mainly affected by DCL4. While the four DCLs are partially redundant for CaLCuV-induced mRNA degradation, DCL4 in conjunction with RDR6 and ..
  7. Bouche N, Lauressergues D, Gasciolli V, Vaucheret H. An antagonistic function for Arabidopsis DCL2 in development and a new function for DCL4 in generating viral siRNAs. EMBO J. 2006;25:3347-56 pubmed
    ..the production of miRNAs and siRNAs by DCL1 in certain circumstances and affects development deleteriously in dcl1 dcl4 and dcl1 dcl3 dcl4 mutant plants, whereas dcl1 dcl2 dcl3 dcl4 quadruple mutant plants are viable...
  8. Qu F, Ye X, Morris T. Arabidopsis DRB4, AGO1, AGO7, and RDR6 participate in a DCL4-initiated antiviral RNA silencing pathway negatively regulated by DCL1. Proc Natl Acad Sci U S A. 2008;105:14732-7 pubmed publisher
    ..We confirm previous reports implicating both DCL4 and DCL2 in this process and establish a minor role for DCL3...
  9. Henderson I, Zhang X, Lu C, Johnson L, Meyers B, Green P, et al. Dissecting Arabidopsis thaliana DICER function in small RNA processing, gene silencing and DNA methylation patterning. Nat Genet. 2006;38:721-5 pubmed
    ..Here, we study the role of the four Arabidopsis thaliana DICER-LIKE genes (DCL1-DCL4) in these processes...

More Information


  1. Dunoyer P, Himber C, Voinnet O. DICER-LIKE 4 is required for RNA interference and produces the 21-nucleotide small interfering RNA component of the plant cell-to-cell silencing signal. Nat Genet. 2005;37:1356-60 pubmed
    ..Five alleles defective in synthesis of 21-nt, but not 24-nt, siRNAs carry mutations in Dicer-like 4 (DCL4) that are involved in biogenesis of trans-acting siRNAs...
  2. Deleris A, Gallego Bartolome J, Bao J, Kasschau K, Carrington J, Voinnet O. Hierarchical action and inhibition of plant Dicer-like proteins in antiviral defense. Science. 2006;313:68-71 pubmed
    ..We show here that virus-derived small RNAs produced by Arabidopsis Dicer-like 4 (DCL4) program an effector complex conferring antiviral immunity...
  3. Xie Z, Allen E, Wilken A, Carrington J. DICER-LIKE 4 functions in trans-acting small interfering RNA biogenesis and vegetative phase change in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 2005;102:12984-9 pubmed
    ..A mutant with defects in DCL4 was identified and analyzed for microRNA- and endogenous, small interfering RNA (siRNA)-related functions...
  4. Kung Y, Lin P, Yeh S, Hong S, Chua N, Liu L, et al. Genetic analyses of the FRNK motif function of Turnip mosaic virus uncover multiple and potentially interactive pathways of cross-protection. Mol Plant Microbe Interact. 2014;27:944-55 pubmed publisher
    ..by a severe strain of TuMV in Nicotiana benthamiana, Arabidopsis thaliana Col-0, and the Arabidopsis dcl2-4/dcl4-1 double mutant defective in DICER-like ribonuclease (DCL)2/DCL4-mediated silencing...
  5. Moissiard G, Parizotto E, Himber C, Voinnet O. Transitivity in Arabidopsis can be primed, requires the redundant action of the antiviral Dicer-like 4 and Dicer-like 2, and is compromised by viral-encoded suppressor proteins. RNA. 2007;13:1268-78 pubmed
    ..Viral suppression of primary siRNA accumulation shows that transitivity can be initiated with minute amounts of DCL4-dependent 21-nucleotide (nt)-long siRNAs, whereas DCL3-dependent 24-nt siRNAs appear dispensable for this process...
  6. Wu L, Mao L, Qi Y. Roles of dicer-like and argonaute proteins in TAS-derived small interfering RNA-triggered DNA methylation. Plant Physiol. 2012;160:990-9 pubmed publisher
    ..activities of SUPPRESSOR OF GENE SILENCING3 (SGS3), RNA-DEPENDENT RNA POLYMERASE6 (RDR6), and DICER-LIKE4 (DCL4), and loaded into ARGONAUTE1 (AGO1) proteins to posttranscriptionally regulate several target genes by messenger ..
  7. Katiyar Agarwal S, Gao S, Vivian Smith A, Jin H. A novel class of bacteria-induced small RNAs in Arabidopsis. Genes Dev. 2007;21:3123-34 pubmed
    ..which is dicer independent and requires PIWI subfamily proteins, generation of AtlsiRNA-1 requires DCL1, DCL4, and the ARGONAUTE subfamily protein AGO7. It also depends on HYL1, HEN1, HST1, RDR6, and Pol IV...
  8. Qin H, Chen F, Huan X, Machida S, Song J, Yuan Y. Structure of the Arabidopsis thaliana DCL4 DUF283 domain reveals a noncanonical double-stranded RNA-binding fold for protein-protein interaction. RNA. 2010;16:474-81 pubmed publisher
    ..Here we report the first structure of the DUF283 domain from the Arabidopsis thaliana DCL4. The DUF283 domain adopts an alpha-beta-beta-beta-alpha topology and resembles the structural similarity to the ..
  9. Seta A, Tabara M, Nishibori Y, Hiraguri A, Ohkama Ohtsu N, Yokoyama T, et al. Post-Translational Regulation of the Dicing Activities of Arabidopsis DICER-LIKE 3 and 4 by Inorganic Phosphate and the Redox State. Plant Cell Physiol. 2017;58:485-495 pubmed publisher
    In Arabidopsis thaliana, small interfering RNAs (siRNAs) generated by two Dicer isoforms, DCL3 and DCL4, function in distinct epigenetic processes, i.e. RNA-directed DNA methylation and post-transcriptional gene silencing, respectively...
  10. Mohannath G, Jackel J, Lee Y, Buchmann R, Wang H, Patil V, et al. A complex containing SNF1-related kinase (SnRK1) and adenosine kinase in Arabidopsis. PLoS ONE. 2014;9:e87592 pubmed publisher
    ..This study establishes the existence of SnRK1-ADK complexes that may play important roles in energy homeostasis and cellular responses to biotic and abiotic stress. ..
  11. Wu Y, Hou B, Lee W, Lu S, Yang C, Vaucheret H, et al. DCL2- and RDR6-dependent transitive silencing of SMXL4 and SMXL5 in Arabidopsis dcl4 mutants causes defective phloem transport and carbohydrate over-accumulation. Plant J. 2017;90:1064-1078 pubmed publisher
    ..Arabidopsis DCL4 and DCL2 each allow the post-transcriptional gene silencing (PTGS) of viruses and transgenes, but primary PTGS-..
  12. Dunoyer P, Voinnet O. Movement of RNA silencing between plant cells: is the question now behind us?. Trends Plant Sci. 2009;14:643-4 pubmed publisher
  13. Liu F, Bakht S, Dean C. Cotranscriptional role for Arabidopsis DICER-LIKE 4 in transcription termination. Science. 2012;335:1621-3 pubmed publisher
    ..We have discovered a role for the Arabidopsis RNA silencing enzyme DICER-LIKE 4 (DCL4) in transcription termination of an endogenous Arabidopsis gene, FCA...
  14. Nagano H, Fukudome A, Hiraguri A, Moriyama H, Fukuhara T. Distinct substrate specificities of Arabidopsis DCL3 and DCL4. Nucleic Acids Res. 2014;42:1845-56 pubmed publisher
    In Arabidopsis thaliana, Dicer-like 3 (DCL3) and Dicer-like 4 (DCL4) cleave long, perfect double-stranded RNAs (dsRNAs) into 24 and 21 nucleotides (nt) small interfering RNAs, respectively, which in turn function in RNA-directed DNA ..
  15. Ma X, Nicole M, Meteignier L, Hong N, Wang G, Moffett P. Different roles for RNA silencing and RNA processing components in virus recovery and virus-induced gene silencing in plants. J Exp Bot. 2015;66:919-32 pubmed publisher
    ..Consistent with this, we found that viral recovery induced increased PB formation and that a decapping mutant (DCP2) showed increased VIGS and virus RNA accumulation, indicating an important role for PBs in eliminating viral RNA. ..
  16. Brosseau C, Moffett P. Functional and Genetic Analysis Identify a Role for Arabidopsis ARGONAUTE5 in Antiviral RNA Silencing. Plant Cell. 2015;27:1742-54 pubmed publisher
    ..In agreement with functional assays, we show that Potexvirus infection induces AGO5 expression and that both AGO2 and AGO5 are required for full restriction of PVX infection in systemic tissues of Arabidopsis. ..
  17. Ziebell H, Carr J. Effects of dicer-like endoribonucleases 2 and 4 on infection of Arabidopsis thaliana by cucumber mosaic virus and a mutant virus lacking the 2b counter-defence protein gene. J Gen Virol. 2009;90:2288-92 pubmed publisher
    ..Cross-protection by Fny-CMVDelta2b against Fny-CMV infection was not abolished in dcl2, dcl3 or dcl4 mutant plants and was strongest in dcl2/4 double mutants, although in these plants and in dcl4 mutants, Fny-..
  18. Mallory A, Vaucheret H. ARGONAUTE 1 homeostasis invokes the coordinate action of the microRNA and siRNA pathways. EMBO Rep. 2009;10:521-6 pubmed publisher
    ..3 (SGS3)- and SILENCING DEFECTIVE 5 (SDE5)-dependent AGO1 silencing, which also requires DICER-LIKE 2 (DCL2) and DCL4. By varying the efficacy of miR168-guided AGO1 mRNA cleavage, we show that siRNA-mediated AGO1 silencing depends on ..
  19. Mickiewicz A, Sarzynska J, Miłostan M, Kurzynska Kokorniak A, Rybarczyk A, Łukasiak P, et al. Modeling of the catalytic core of Arabidopsis thaliana Dicer-like 4 protein and its complex with double-stranded RNA. Comput Biol Chem. 2017;66:44-56 pubmed publisher
    ..Our studies focus on AtDCL4 that processes double-stranded RNAs (dsRNAs) into 21 nucleotide trans-acting small interfering RNAs...
  20. Jakubiec A, Yang S, Chua N. Arabidopsis DRB4 protein in antiviral defense against Turnip yellow mosaic virus infection. Plant J. 2012;69:14-25 pubmed publisher
    RNA silencing is an important antiviral mechanism in diverse eukaryotic organisms. In Arabidopsis DICER-LIKE?4 (DCL4) is the primary antiviral Dicer, required for the production of viral small RNAs from positive-strand RNA viruses...
  21. Lewsey M, Carr J. Effects of DICER-like proteins 2, 3 and 4 on cucumber mosaic virus and tobacco mosaic virus infections in salicylic acid-treated plants. J Gen Virol. 2009;90:3010-4 pubmed publisher
    ..However, the findings do not exclude RNA silencing from making a contribution to SA-mediated resistance in wild-type plants...
  22. Zhang S, Liu Y, Yu B. PRL1, an RNA-binding protein, positively regulates the accumulation of miRNAs and siRNAs in Arabidopsis. PLoS Genet. 2014;10:e1004841 pubmed publisher
    ..Furthermore, PRL1 interacts with the pri-miRNA processor, DCL1, and the dsRNA processors (DCL3 and DCL4). These results suggest that PRL1 may function as a general factor to promote the production of miRNAs and siRNAs...
  23. Howell M, Fahlgren N, Chapman E, Cumbie J, Sullivan C, Givan S, et al. Genome-wide analysis of the RNA-DEPENDENT RNA POLYMERASE6/DICER-LIKE4 pathway in Arabidopsis reveals dependency on miRNA- and tasiRNA-directed targeting. Plant Cell. 2007;19:926-42 pubmed
    ..many endogenous transcripts, viruses, and transgenes involves the RNA-DEPENDENT RNA POLYMERASE6/DICER-LIKE4 (RDR6/DCL4)-dependent short interfering RNA (siRNA) biogenesis pathway...
  24. Fang X, Shi Y, Lu X, Chen Z, Qi Y. CMA33/XCT Regulates Small RNA Production through Modulating the Transcription of Dicer-Like Genes in Arabidopsis. Mol Plant. 2015;8:1227-36 pubmed publisher
    ..of sRNAs in Arabidopsis: DCL1-dependent microRNA (miRNA), DCL3-dependent heterochromatic siRNA (hc-siRNA), and DCL4-dependent trans-acting siRNA (ta-siRNA)...