Gene Symbol: DBE1
Description: debranching enzyme 1
Alias: ARABIDOPSIS THALIANA ISOAMYLASE 2, ATISA2, AtBE2, BE2, BRANCHING ENZYME 2, F15K9.9, F15K9_9, ISA2, debranching enzyme 1, debranching enzyme 1
Species: thale cress
Delatte T, Trevisan M, Parker M, Zeeman S. Arabidopsis mutants Atisa1 and Atisa2 have identical phenotypes and lack the same multimeric isoamylase, which influences the branch point distribution of amylopectin during starch synthesis. Plant J. 2005;41:815-30 pubmed
..The same activity is abolished by mutation at the DBE1 locus, which encodes a second isoamylase-type protein, AtISA2. This is consistent with the idea that ISA1 and ISA2 proteins are subunits of the same enzyme in vivo...
Streb S, Delatte T, Umhang M, Eicke S, Schorderet M, Reinhardt D, et al
. Starch granule biosynthesis in Arabidopsis is abolished by removal of all debranching enzymes but restored by the subsequent removal of an endoamylase. Plant Cell. 2008;20:3448-66 pubmed publisher
..Quadruple mutants lacking all four DBE proteins (Isoamylase1 [ISA1], ISA2, and ISA3, and Limit-Dextrinase) are devoid of starch granules and instead accumulate highly branched glucans, ..
Facon M, Lin Q, Azzaz A, Hennen Bierwagen T, Myers A, Putaux J, et al
. Distinct functional properties of isoamylase-type starch debranching enzymes in monocot and dicot leaves. Plant Physiol. 2013;163:1363-75 pubmed publisher
..organisms, as shown by the strict conservation of both catalytically active ISA1 and the noncatalytic homolog ISA2. Functional distinctions exist between species, although they are not understood yet...
Streb S, Zeeman S. Replacement of the endogenous starch debranching enzymes ISA1 and ISA2 of Arabidopsis with the rice orthologs reveals a degree of functional conservation during starch synthesis. PLoS ONE. 2014;9:e92174 pubmed publisher
..differences in amylopectin structure by replacing the dicotyledonous Arabidopsis isoamylases (AtISA1 and AtISA2) with the monocotyledonous rice (Oryza sativa) isoforms...
Wattebled F, Planchot V, Dong Y, Szydlowski N, Pontoire B, Devin A, et al
. Further evidence for the mandatory nature of polysaccharide debranching for the aggregation of semicrystalline starch and for overlapping functions of debranching enzymes in Arabidopsis leaves. Plant Physiol. 2008;148:1309-23 pubmed publisher
..of debranching enzymes are found in the genome of Arabidopsis (Arabidopsis thaliana): three isoamylases (ISA1, ISA2, and ISA3) and a pullulanase (PU1)...
Sundberg M, Pfister B, Fulton D, Bischof S, Delatte T, Eicke S, et al
. The heteromultimeric debranching enzyme involved in starch synthesis in Arabidopsis requires both isoamylase1 and isoamylase2 subunits for complex stability and activity. PLoS ONE. 2013;8:e75223 pubmed publisher
..affinity purification) tagging to provide direct evidence in Arabidopsis that ISA1 interacts with its homolog ISA2. No evidence for interaction with other starch biosynthetic enzymes was found...