Gene Symbol: CIB1
Description: cryptochrome-interacting basic-helix-loop-helix 1
Alias: T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1, cryptochrome-interacting basic-helix-loop-helix 1
Species: thale cress
Products:     CIB1

Top Publications

  1. Li X, Wang Q, Yu X, Liu H, Yang H, Zhao C, et al. Arabidopsis cryptochrome 2 (CRY2) functions by the photoactivation mechanism distinct from the tryptophan (trp) triad-dependent photoreduction. Proc Natl Acad Sci U S A. 2011;108:20844-9 pubmed publisher
    ..with the CRY2-signaling proteins SUPPRESSOR OF PHYA 1 (SPA1) and cryptochrome-interaction basic helix-loop-helix 1 (CIB1), the constitutively active CRY2(W374A) interacts with SPA1 and CIB1 constitutively...
  2. Liu H, Yu X, Li K, Klejnot J, Yang H, Lisiero D, et al. Photoexcited CRY2 interacts with CIB1 to regulate transcription and floral initiation in Arabidopsis. Science. 2008;322:1535-9 pubmed publisher
    ..We report here the identification and characterization of the Arabidopsis CIB1 (cryptochrome-interacting basic-helix-loop-helix) protein...
  3. Kennedy M, Hughes R, Peteya L, Schwartz J, Ehlers M, Tucker C. Rapid blue-light-mediated induction of protein interactions in living cells. Nat Methods. 2010;7:973-5 pubmed publisher
    ..genetically encoded light-inducible protein-interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1 that require no exogenous ligands and dimerize on blue-light exposure with subsecond time resolution and ..
  4. Taslimi A, Zoltowski B, Miranda J, Pathak G, Hughes R, Tucker C. Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase. Nat Chem Biol. 2016;12:425-30 pubmed publisher
    ..adapted for use in controlling protein-protein interactions through light-dependent binding to a partner protein, CIB1. While the existing CRY2-CIB dimerization system has been used extensively for optogenetic applications, some ..
  5. Jeong R, Chandra Shekara A, Barman S, Navarre D, Klessig D, Kachroo A, et al. Cryptochrome 2 and phototropin 2 regulate resistance protein-mediated viral defense by negatively regulating an E3 ubiquitin ligase. Proc Natl Acad Sci U S A. 2010;107:13538-43 pubmed publisher
    ..We propose that CRY2/PHOT2 negatively regulate the proteasome-mediated degradation of HRT, likely via COP1, and blue-light relieves this repression resulting in HRT degradation...
  6. Weidler G, Zur Oven Krockhaus S, Heunemann M, Orth C, Schleifenbaum F, Harter K, et al. Degradation of Arabidopsis CRY2 is regulated by SPA proteins and phytochrome A. Plant Cell. 2012;24:2610-23 pubmed publisher
    ..Our results suggest that cry2 stability is controlled by SPA and phyA, thus providing more information on the molecular mechanisms of interaction between cryptochrome and phytochrome photoreceptors. ..
  7. Hughes R, Bolger S, Tapadia H, Tucker C. Light-mediated control of DNA transcription in yeast. Methods. 2012;58:385-91 pubmed publisher
    ..Light is dose dependent, inexpensive to apply, easily delivered, and does not interfere with cellular pathways, and thus has significant advantages over chemical systems. ..
  8. Duan L, Hope J, Ong Q, Lou H, Kim N, McCarthy C, et al. Understanding CRY2 interactions for optical control of intracellular signaling. Nat Commun. 2017;8:547 pubmed publisher
    Arabidopsis cryptochrome 2 (CRY2) can simultaneously undergo light-dependent CRY2-CRY2 homo-oligomerization and CRY2-CIB1 hetero-dimerization, both of which have been widely used to optically control intracellular processes...
  9. Liu H, Wang Q, Liu Y, Zhao X, Imaizumi T, Somers D, et al. Arabidopsis CRY2 and ZTL mediate blue-light regulation of the transcription factor CIB1 by distinct mechanisms. Proc Natl Acad Sci U S A. 2013;110:17582-7 pubmed publisher
    ..cryptochrome 2 interacts with the transcription factor CRYPTOCHROME-INTERACTING basic helix-loop-helix 1 (CIB1) to activate transcription and floral initiation...

More Information


  1. Liu Y, Li X, Li K, Liu H, Lin C. Multiple bHLH proteins form heterodimers to mediate CRY2-dependent regulation of flowering-time in Arabidopsis. PLoS Genet. 2013;9:e1003861 pubmed publisher
    Arabidopsis thaliana cryptochrome 2 (CRY2) mediates light control of flowering time. CIB1 (CRY2-interacting bHLH 1) specifically interacts with CRY2 in response to blue light to activate the transcription of FT (Flowering Locus T)...
  2. Cui Y, Choudhury S, Irudayaraj J. Quantitative real-time kinetics of optogenetic proteins CRY2 and CIB1/N using single-molecule tools. Anal Biochem. 2014;458:58-60 pubmed publisher
    In this work we evaluate the interaction of two optogenetic protein variants (CIB1, CIBN) with their complementary protein CRY2 by single-molecule tools in cell-free extracts...