Gene Symbol: CCA1
Description: circadian clock associated 1
Alias: AtCCA1, F19D11.11, MYB-RELATED DNA BINDING PROTEIN, circadian clock associated 1, circadian clock associated 1
Species: thale cress

Top Publications

  1. Wang Z, Tobin E. Constitutive expression of the CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) gene disrupts circadian rhythms and suppresses its own expression. Cell. 1998;93:1207-17 pubmed
    The CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) gene encodes a MYB-related transcription factor involved in the phytochrome induction of a light-harvesting chlorophyll a/b-protein (Lhcb) gene...
  2. Pokhilko A, Fernández A, Edwards K, Southern M, Halliday K, Millar A. The clock gene circuit in Arabidopsis includes a repressilator with additional feedback loops. Mol Syst Biol. 2012;8:574 pubmed publisher
    ..As well as matching timeseries and phase-response data, the model provides a new conceptual framework for the plant clock that includes a three-component repressilator circuit in its complex structure. ..
  3. Portolés S, Mas P. The functional interplay between protein kinase CK2 and CCA1 transcriptional activity is essential for clock temperature compensation in Arabidopsis. PLoS Genet. 2010;6:e1001201 pubmed publisher
    ..a previously unpredicted mechanism for CK2 antagonizing the key clock regulator CIRCADIAN CLOCK-ASSOCIATED 1 (CCA1)...
  4. Daniel X, Sugano S, Tobin E. CK2 phosphorylation of CCA1 is necessary for its circadian oscillator function in Arabidopsis. Proc Natl Acad Sci U S A. 2004;101:3292-7 pubmed
    ..shown that in Arabidopsis, the protein kinase CK2 can phosphorylate the clock-associated protein CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) in vitro...
  5. Mizoguchi T, Wheatley K, Hanzawa Y, Wright L, Mizoguchi M, Song H, et al. LHY and CCA1 are partially redundant genes required to maintain circadian rhythms in Arabidopsis. Dev Cell. 2002;2:629-41 pubmed
    ..LHY and CCA1 are related MYB-like transcription factors proposed to be closely involved...
  6. Wang Z, Kenigsbuch D, Sun L, Harel E, Ong M, Tobin E. A Myb-related transcription factor is involved in the phytochrome regulation of an Arabidopsis Lhcb gene. Plant Cell. 1997;9:491-507 pubmed
    We have isolated the gene for a protein designated CCA1. This protein can bind to a region of the promoter of an Arabidopsis light-harvesting chlorophyll a/b protein gene, Lhcb1*3, which is necessary for its regulation by phytochrome...
  7. Sugano S, Andronis C, Green R, Wang Z, Tobin E. Protein kinase CK2 interacts with and phosphorylates the Arabidopsis circadian clock-associated 1 protein. Proc Natl Acad Sci U S A. 1998;95:11020-5 pubmed
    The circadian clock-associated 1 (CCA1) gene encodes a Myb-related transcription factor that has been shown to be involved in the phytochrome regulation of Lhcb1*3 gene expression and in the function of the circadian oscillator in ..
  8. Sugano S, Andronis C, Ong M, Green R, Tobin E. The protein kinase CK2 is involved in regulation of circadian rhythms in Arabidopsis. Proc Natl Acad Sci U S A. 1999;96:12362-6 pubmed
    ..The circadian clock-associated 1 (CCA1) and the late elongated hypocotyl (LHY) proteins have been shown to be closely associated with clock function in ..
  9. Farré E, Harmer S, Harmon F, Yanovsky M, Kay S. Overlapping and distinct roles of PRR7 and PRR9 in the Arabidopsis circadian clock. Curr Biol. 2005;15:47-54 pubmed
    ..In plants, the first suggested regulatory loop involves the transcription factors CIRCADIAN CLOCK-ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) and the pseudo-response regulator TIMING OF CAB EXPRESSION 1 (TOC1/PRR1)...

More Information


  1. Kikis E, Khanna R, Quail P. ELF4 is a phytochrome-regulated component of a negative-feedback loop involving the central oscillator components CCA1 and LHY. Plant J. 2005;44:300-13 pubmed
    ..has been presented that a negative transcriptional feedback loop formed by the genes CIRCADIAN CLOCK ASSOCIATED (CCA1), LATE ELONGATED HYPOCOTYL (LHY) and TIMING OF CAB (TOC1) constitutes the core of the central oscillator of the ..
  2. Ding Z, Doyle M, Amasino R, Davis S. A complex genetic interaction between Arabidopsis thaliana TOC1 and CCA1/LHY in driving the circadian clock and in output regulation. Genetics. 2007;176:1501-10 pubmed
    It has been proposed that CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) together with TIMING OF CAB EXPRESSION 1 (TOC1) make up the central oscillator of the Arabidopsis thaliana circadian clock...
  3. Alabadi D, Oyama T, Yanovsky M, Harmon F, Mas P, Kay S. Reciprocal regulation between TOC1 and LHY/CCA1 within the Arabidopsis circadian clock. Science. 2001;293:880-3 pubmed
    ..Here, we show interactions between the Arabidopsis clock genes LATE ELONGATED HYPOCOTYL (LHY), CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), and TIMING OF CAB EXPRESSION 1 (TOC1)...
  4. Li G, Siddiqui H, Teng Y, Lin R, Wan X, Li J, et al. Coordinated transcriptional regulation underlying the circadian clock in Arabidopsis. Nat Cell Biol. 2011;13:616-22 pubmed publisher
    ..component of the central oscillator, and activate its expression during the day, whereas the circadian-controlled CCA1 and LHY proteins directly suppress ELF4 expression periodically at dawn through physical interactions with these ..
  5. Yakir E, Hilman D, Kron I, Hassidim M, Melamed Book N, Green R. Posttranslational regulation of CIRCADIAN CLOCK ASSOCIATED1 in the circadian oscillator of Arabidopsis. Plant Physiol. 2009;150:844-57 pubmed publisher
    ..CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and LATE ELONGATED HYPOCOTYLS (LHY) are closely related single myb transcription factors that have been identified ..
  6. Ovadia A, Tabibian Keissar H, Cohen Y, Kenigsbuch D. The 5'UTR of CCA1 includes an autoregulatory cis element that segregates between light and circadian regulation of CCA1 and LHY. Plant Mol Biol. 2010;72:659-71 pubmed publisher
    The transcription factor CCA1 (CIRCADIAN CLOCK ASSOCIATED 1) participates in both light and circadian clock regulation in Arabidopsis. Two sets of transgenic plants in which GFP was fused to the CCA1 promoter with (1...
  7. Hsu P, Devisetty U, Harmer S. Accurate timekeeping is controlled by a cycling activator in Arabidopsis. elife. 2013;2:e00473 pubmed publisher
    ..Further, our work suggests that the plant clock consists of a highly interconnected, complex regulatory network rather than of coupled morning and evening feedback loops. DOI: ..
  8. Nagel D, Doherty C, Pruneda Paz J, Schmitz R, Ecker J, Kay S. Genome-wide identification of CCA1 targets uncovers an expanded clock network in Arabidopsis. Proc Natl Acad Sci U S A. 2015;112:E4802-10 pubmed publisher
    ..As a key component of the clock, the Myb-like transcription factor CIRCADIAN CLOCK ASSOCIATED1 (CCA1) is able to initiate and set the phase of clock-controlled rhythms and has been shown to regulate gene expression ..
  9. Lu S, Webb C, Knowles S, Kim S, Wang Z, Tobin E. CCA1 and ELF3 Interact in the control of hypocotyl length and flowering time in Arabidopsis. Plant Physiol. 2012;158:1079-88 pubmed publisher
    ..CIRCADIAN CLOCK ASSOCIATED1 (CCA1) is a central clock component, and CCA1 overexpression causes circadian dysfunction, elongated hypocotyls, and late ..
  10. Miyata K, Calviño M, Oda A, Sugiyama H, Mizoguchi T. Suppression of late-flowering and semi-dwarf phenotypes in the Arabidopsis clock mutant lhy-12;cca1-101 by phyB under continuous light. Plant Signal Behav. 2011;6:1162-71 pubmed publisher
    ..Double mutations in LHY and CCA1 (lhy;cca1) accelerated flowering under short days, mainly by the GI-CO dependent pathway...
  11. Wang W, Barnaby J, Tada Y, Li H, Tör M, Caldelari D, et al. Timing of plant immune responses by a central circadian regulator. Nature. 2011;470:110-4 pubmed publisher
    ..downy mildew in Arabidopsis and their regulatory control by the circadian regulator, CIRCADIAN CLOCK-ASSOCIATED 1 (CCA1)...
  12. Delis C, Krokida A, Tomatsidou A, Tsikou D, Beta R, Tsioumpekou M, et al. AtHESPERIN: a novel regulator of circadian rhythms with poly(A)-degrading activity in plants. RNA Biol. 2016;13:68-82 pubmed publisher
    ..of AtHESP in plant lines affects the expression and rhythmicity of the clock core oscillator genes TOC1 and CCA1. This study demonstrates an evolutionary conserved poly(A)-degrading activity in plants and suggests deadenylation ..
  13. Lai A, Doherty C, Mueller Roeber B, Kay S, Schippers J, Dijkwel P. CIRCADIAN CLOCK-ASSOCIATED 1 regulates ROS homeostasis and oxidative stress responses. Proc Natl Acad Sci U S A. 2012;109:17129-34 pubmed publisher
    ..Mutations in the core-clock regulator, CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), affect the transcriptional regulation of ROS-responsive genes, ROS homeostasis, and tolerance ..
  14. Seo P, Park M, Lim M, Kim S, Lee M, Baldwin I, et al. A self-regulatory circuit of CIRCADIAN CLOCK-ASSOCIATED1 underlies the circadian clock regulation of temperature responses in Arabidopsis. Plant Cell. 2012;24:2427-42 pubmed publisher
    ..Clock components, including CIRCADIAN CLOCK-ASSOCIATED1 (CCA1), are also associated with cold acclimation...
  15. Kamioka M, Takao S, Suzuki T, Taki K, Higashiyama T, Kinoshita T, et al. Direct Repression of Evening Genes by CIRCADIAN CLOCK-ASSOCIATED1 in the Arabidopsis Circadian Clock. Plant Cell. 2016;28:696-711 pubmed publisher
    ..Here, we demonstrate that direct regulation of PRR5 by CIRCADIAN CLOCK-ASSOCIATED1 (CCA1) determines the repression state of PRR5 in the morning...
  16. Ito S, Nakamichi N, Nakamura Y, Niwa Y, Kato T, Murakami M, et al. Genetic linkages between circadian clock-associated components and phytochrome-dependent red light signal transduction in Arabidopsis thaliana. Plant Cell Physiol. 2007;48:971-83 pubmed
    The current best candidates for Arabidopsis thaliana clock components are CCA1 (CIRCADIAN CLOCK-ASSOCIATED 1) and its homolog LHY (LATE ELONGATED HYPOCOTYL)...
  17. Kawamura M, Ito S, Nakamichi N, Yamashino T, Mizuno T. The function of the clock-associated transcriptional regulator CCA1 (CIRCADIAN CLOCK-ASSOCIATED 1) in Arabidopsis thaliana. Biosci Biotechnol Biochem. 2008;72:1307-16 pubmed
    In the model higher plant Arabidopsis thaliana, the CCA1 (CIRCADIAN CLOCK-ASSOCIATED 1) gene plays important circadian clock-associated roles. The CCA1 protein is a member of a small subfamily of single MYB-related transcription factors...
  18. Lau O, Huang X, Charron J, Lee J, Li G, Deng X. Interaction of Arabidopsis DET1 with CCA1 and LHY in mediating transcriptional repression in the plant circadian clock. Mol Cell. 2011;43:703-12 pubmed publisher
    ..transcriptional repression activity and physically interacts with two closely related MYB transcription factors, CCA1 and LHY, which are core components of the plant circadian clock...
  19. Dai S, Wei X, Pei L, Thompson R, Liu Y, Heard J, et al. BROTHER OF LUX ARRHYTHMO is a component of the Arabidopsis circadian clock. Plant Cell. 2011;23:961-72 pubmed publisher
    ..Arabidopsis circadian clock protein CIRCADIAN CLOCK ASSOCIATED1 (CCA1) binds to the evening element of the BOA promoter and negatively regulates its expression...
  20. Niwa Y, Ito S, Nakamichi N, Mizoguchi T, Niinuma K, Yamashino T, et al. Genetic linkages of the circadian clock-associated genes, TOC1, CCA1 and LHY, in the photoperiodic control of flowering time in Arabidopsis thaliana. Plant Cell Physiol. 2007;48:925-37 pubmed
    ..Nevertheless, the genetic linkages between the major clock-associated TOC1, CCA1 and LHY genes and the canonical CO-FT flowering pathway are less clear...
  21. Yoshida R, Fekih R, Fujiwara S, Oda A, Miyata K, Tomozoe Y, et al. Possible role of early flowering 3 (ELF3) in clock-dependent floral regulation by short vegetative phase (SVP) in Arabidopsis thaliana. New Phytol. 2009;182:838-50 pubmed publisher
    ..Double mutations in two clock genes, late elongated hypocotyl (LHY) and circadian clock associated 1 (CCA1), accelerated flowering under short days (SDs) but delayed flowering under continuous light (LL) ..
  22. Andronis C, Barak S, Knowles S, Sugano S, Tobin E. The clock protein CCA1 and the bZIP transcription factor HY5 physically interact to regulate gene expression in Arabidopsis. Mol Plant. 2008;1:58-67 pubmed publisher
    ..of these Arabidopsis genes--Lhcb1*1 and Lhcb1*3--and identified a sequence that binds the clock protein CIRCADIAN CLOCK ASSOCIATED 1 (CCA1)...
  23. Giraud E, Ng S, Carrie C, Duncan O, Low J, Lee C, et al. TCP transcription factors link the regulation of genes encoding mitochondrial proteins with the circadian clock in Arabidopsis thaliana. Plant Cell. 2010;22:3921-34 pubmed publisher
  24. Thain S, Vandenbussche F, Laarhoven L, Dowson Day M, Wang Z, Tobin E, et al. Circadian rhythms of ethylene emission in Arabidopsis. Plant Physiol. 2004;136:3751-61 pubmed
    ..In addition, ethylene production is controlled by the TIMING OF CAB EXPRESSION 1 and CIRCADIAN CLOCK ASSOCIATED 1 genes, which are critical for all circadian rhythms yet tested in Arabidopsis...
  25. Lee H, Lee K, Jang K, Seo P. Circadian expression profiles of chromatin remodeling factor genes in Arabidopsis. J Plant Res. 2015;128:187-99 pubmed publisher
  26. Dong M, Farré E, Thomashow M. Circadian clock-associated 1 and late elongated hypocotyl regulate expression of the C-repeat binding factor (CBF) pathway in Arabidopsis. Proc Natl Acad Sci U S A. 2011;108:7241-6 pubmed publisher
    ..e., Circadian Clock-Associated 1 (CCA1) and Late Elongated Hypocotyl (LHY)...
  27. Gutierrez R, Stokes T, Thum K, Xu X, Obertello M, Katari M, et al. Systems approach identifies an organic nitrogen-responsive gene network that is regulated by the master clock control gene CCA1. Proc Natl Acad Sci U S A. 2008;105:4939-44 pubmed publisher
    ..integrated network model of molecular interactions, we uncovered a subnetwork regulated by organic N that included CCA1 and target genes involved in N-assimilation...
  28. Salome P, Weigel D, McClung C. The role of the Arabidopsis morning loop components CCA1, LHY, PRR7, and PRR9 in temperature compensation. Plant Cell. 2010;22:3650-61 pubmed publisher
    ..are overcompensated, apparently due to hyperactivation of the transcription factors CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY)...
  29. Nakamichi N, Kita M, Niinuma K, Ito S, Yamashino T, Mizoguchi T, et al. Arabidopsis clock-associated pseudo-response regulators PRR9, PRR7 and PRR5 coordinately and positively regulate flowering time through the canonical CONSTANS-dependent photoperiodic pathway. Plant Cell Physiol. 2007;48:822-32 pubmed
    ..Then, extensive genetic studies using CIRCADIAN CLOCK-ASSOCIATED1 (CCA1)/LATE ELONGATED HYPOCOTYL (LHY) double mutants (cca1/lhy) and prr7/prr5 were conducted...
  30. Lu S, Knowles S, Andronis C, Ong M, Tobin E. CIRCADIAN CLOCK ASSOCIATED1 and LATE ELONGATED HYPOCOTYL function synergistically in the circadian clock of Arabidopsis. Plant Physiol. 2009;150:834-43 pubmed publisher
    ..CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) are Myb-related proteins that function in or close to the central oscillator in ..
  31. Song H, Noh Y. Rhythmic oscillation of histone acetylation and methylation at the Arabidopsis central clock loci. Mol Cells. 2012;34:279-87 pubmed
    ..In Arabidopsis, LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) transcripts are highly expressed in the morning...
  32. Fujimori T, Sato E, Yamashino T, Mizuno T. PRR5 (PSEUDO-RESPONSE REGULATOR 5) plays antagonistic roles to CCA1 (CIRCADIAN CLOCK-ASSOCIATED 1) in Arabidopsis thaliana. Biosci Biotechnol Biochem. 2005;69:426-30 pubmed
    ..In the model higher plant Arabidopsis thaliana, the best candidates for clock components are CCA1 (CIRCADIAN CLOCK-ASSOCIATED 1) and LHY (LATE ELONGATED HYPOCOTYL), which are homologous Myb-related transcription ..
  33. Shi H, Wei Y, He C. Melatonin-induced CBF/DREB1s are essential for diurnal change of disease resistance and CCA1 expression in Arabidopsis. Plant Physiol Biochem. 2016;100:150-155 pubmed publisher
    ..Notably, the diurnal changes of endogenous melatonin may regulate corresponding changes of AtCBF/DREB1s expression and their underlying diurnal cycle of plant immunity and AtCCA1.
  34. Higham C, Husmeier D. A Bayesian approach for parameter estimation in the extended clock gene circuit of Arabidopsis thaliana. BMC Bioinformatics. 2013;14 Suppl 10:S3 pubmed publisher
  35. Xie Q, Wang P, Liu X, Yuan L, Wang L, Zhang C, et al. LNK1 and LNK2 are transcriptional coactivators in the Arabidopsis circadian oscillator. Plant Cell. 2014;26:2843-57 pubmed publisher
    ..We conclude that LNK1 is a transcriptional coactivator necessary for expression of the clock genes PRR5 and TOC1 through recruitment to their promoters via interaction with bona fide DNA binding proteins such as RVE4 and RVE8. ..
  36. Yakir E, Hilman D, Hassidim M, Green R. CIRCADIAN CLOCK ASSOCIATED1 transcript stability and the entrainment of the circadian clock in Arabidopsis. Plant Physiol. 2007;145:925-32 pubmed the light-mediated regulation of expression of one of the oscillator genes, CIRCADIAN CLOCK ASSOCIATED1 (CCA1)...
  37. Su Y, Wang S, Zhang F, Zheng H, Liu Y, Huang T, et al. Phosphorylation of Histone H2A at Serine 95: A Plant-Specific Mark Involved in Flowering Time Regulation and H2A.Z Deposition. Plant Cell. 2017;29:2197-2213 pubmed publisher
    ..MLK4 interacts with CIRCADIAN CLOCK ASSOCIATED1 (CCA1), which allows MLK4 to bind to the GIGANTEA (GI) promoter...
  38. Ueoka Nakanishi H, Yamashino T, Ishida K, Kamioka M, Nakamichi N, Mizuno T. Molecular mechanisms of circadian rhythm in Lotus japonicus and Arabidopsis thaliana are sufficiently compatible to regulate heterologous core clock genes robustly. Biosci Biotechnol Biochem. 2012;76:2332-4 pubmed
    ..thaliana core clock genes CCA1 and PRR5 in heterologous L. japonicus cells and found that the molecular mechanism of circadian rhythm in L...
  39. Ogiso E, Takahashi Y, Sasaki T, Yano M, Izawa T. The role of casein kinase II in flowering time regulation has diversified during evolution. Plant Physiol. 2010;152:808-20 pubmed publisher
    ..The circadian clock component CIRCADIAN CLOCK ASSOCIATED1 (CCA1) is a CK2 target in Arabidopsis, where it influences photoperiodic flowering...
  40. Fujiwara S, Oda A, Yoshida R, Niinuma K, Miyata K, Tomozoe Y, et al. Circadian clock proteins LHY and CCA1 regulate SVP protein accumulation to control flowering in Arabidopsis. Plant Cell. 2008;20:2960-71 pubmed publisher
    ..we demonstrate that the circadian clock proteins LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK-ASSOCIATED1 (CCA1) not only repressed the floral transition under short-day and long-day conditions but also accelerated flowering ..
  41. Ding Z, Millar A, Davis A, Davis S. TIME FOR COFFEE encodes a nuclear regulator in the Arabidopsis thaliana circadian clock. Plant Cell. 2007;19:1522-36 pubmed
    ..Neither its abundance nor its cellular distribution was found to be clock regulated. We suggest that TIC encodes a nucleus-acting clock regulator working close to the central oscillator. ..
  42. Hemmes H, Henriques R, Jang I, Kim S, Chua N. Circadian clock regulates dynamic chromatin modifications associated with Arabidopsis CCA1/LHY and TOC1 transcriptional rhythms. Plant Cell Physiol. 2012;53:2016-29 pubmed publisher
    ..The Arabidopsis central oscillator contains three genes encoding core clock components. CIRCADIAN CLOCK ASSOCIATED 1 (CCA1)/LATE ELONGATED HYPOCOTYL (LHY) and TIMING OF CAB EXPRESSION 1 (TOC1) reciprocally repress genes ..
  43. Park M, Seo P, Park C. CCA1 alternative splicing as a way of linking the circadian clock to temperature response in Arabidopsis. Plant Signal Behav. 2012;7:1194-6 pubmed publisher
    ..CIRCADIAN CLOCK-ASSOCIATED1 (CCA1) is one of the core clock components in Arabidopsis. Notably, it is also associated with cold acclimation...
  44. Barajas López J, Serrato A, Cazalis R, Meyer Y, Chueca A, Reichheld J, et al. Circadian regulation of chloroplastic f and m thioredoxins through control of the CCA1 transcription factor. J Exp Bot. 2011;62:2039-51 pubmed publisher
    ..Binding shift assays indicate that this control probably involves the interaction of the CCA1 transcription factor and an evening element (EE) located in the PsTRX f and PsTRX m1 promoters...