CBL2

Summary

Gene Symbol: CBL2
Description: calcineurin B-like protein 2
Alias: ATCBL2, MDA7.3, MDA7_3, SCaBP1, SOS3-LIKE CALCIUM BINDING PROTEIN 1, calcineurin B-like protein 2, calcineurin B-like protein 2
Species: thale cress
Products:     CBL2

Top Publications

  1. Nozawa A, Sawada Y, Akiyama T, Koizumi N, Sano H. Variable interactions between sucrose non-fermented 1-related protein kinases and regulatory proteins in higher plants. Biosci Biotechnol Biochem. 2003;67:2533-40 pubmed
    ..This was also the case for AtWL1 and AtWL2, showing an efficient interaction with AtCBL2. In contrast, WPK4 interacted with none of the CBLs...
  2. Batistic O, Sorek N, Schültke S, Yalovsky S, Kudla J. Dual fatty acyl modification determines the localization and plasma membrane targeting of CBL/CIPK Ca2+ signaling complexes in Arabidopsis. Plant Cell. 2008;20:1346-62 pubmed publisher
    ..Our findings demonstrate the importance of S-acylation for regulating the spatial accuracy of Ca2+-decoding proteins and suggest a novel mechanism that enables the functional specificity of calcium sensor/kinase complexes. ..
  3. Fuglsang A, Guo Y, Cuin T, Qiu Q, Song C, Kristiansen K, et al. Arabidopsis protein kinase PKS5 inhibits the plasma membrane H+ -ATPase by preventing interaction with 14-3-3 protein. Plant Cell. 2007;19:1617-34 pubmed
    ..We show that PKS5 interacts with the calcium binding protein SCaBP1 and that high external pH can trigger an increase in the concentration of cytosolic-free calcium...
  4. Yan J, Niu F, Liu W, Zhang H, Wang B, Lan W, et al. Arabidopsis CIPK14 positively regulates glucose response. Biochem Biophys Res Commun. 2014;450:1679-83 pubmed publisher
    ..1 and SnRK1.2 implicated in glucose signaling. This is the first report to demonstrate that CIPK also plays a role in glucose signaling. ..
  5. Xie C, Zhou X, Deng X, Guo Y. PKS5, a SNF1-related kinase, interacts with and phosphorylates NPR1, and modulates expression of WRKY38 and WRKY62. J Genet Genomics. 2010;37:359-69 pubmed publisher
    ..Our results suggest that PKS5 functions at the upstream of NPR1 and might mediate expression of WRKY38 and WRKY62 possibly by interacting with and phosphorylating NPR1. ..
  6. Du W, Lin H, Chen S, Wu Y, Zhang J, Fuglsang A, et al. Phosphorylation of SOS3-like calcium-binding proteins by their interacting SOS2-like protein kinases is a common regulatory mechanism in Arabidopsis. Plant Physiol. 2011;156:2235-43 pubmed publisher
    ..The phosphorylation site serine-216 at the C terminus of SCaBP1 by PKS24 was identified by liquid chromatography-quadrupole mass spectrometry analysis...
  7. Nozawa A, Koizumi N, Sano H. An Arabidopsis SNF1-related protein kinase, AtSR1, interacts with a calcium-binding protein, AtCBL2, of which transcripts respond to light. Plant Cell Physiol. 2001;42:976-81 pubmed
    ..and six calcineurin B like proteins of Arabidopsis and found that AtSR1 prominently interacts with one of them, AtCBL2, by yeast two-hybrid assay...
  8. Lan W, Lee S, Che Y, Jiang Y, Luan S. Mechanistic analysis of AKT1 regulation by the CBL-CIPK-PP2CA interactions. Mol Plant. 2011;4:527-36 pubmed publisher
    ..These findings provide further insights into the signaling network consisting of CBL-CIPK-PP2C interactions in the activation of the AKT1 channel. ..
  9. Eckert C, Offenborn J, Heinz T, Armarego Marriott T, Schültke S, Zhang C, et al. The vacuolar calcium sensors CBL2 and CBL3 affect seed size and embryonic development in Arabidopsis thaliana. Plant J. 2014;78:146-56 pubmed publisher
    ..In this study, we analyzed single and double mutants of the closely related tonoplast-localized calcium sensors CBL2 and CBL3, which show either reduction of function (rf) or complete loss of function (lf)...
  10. Shi J, Kim K, Ritz O, Albrecht V, Gupta R, Harter K, et al. Novel protein kinases associated with calcineurin B-like calcium sensors in Arabidopsis. Plant Cell. 1999;11:2393-405 pubmed
    ..These findings link a new type of Ca(2)+ sensors to a group of novel protein kinases, providing the molecular basis for a unique Ca(2)+ signaling machinery in plant cells. ..

Detail Information

Publications22

  1. Nozawa A, Sawada Y, Akiyama T, Koizumi N, Sano H. Variable interactions between sucrose non-fermented 1-related protein kinases and regulatory proteins in higher plants. Biosci Biotechnol Biochem. 2003;67:2533-40 pubmed
    ..This was also the case for AtWL1 and AtWL2, showing an efficient interaction with AtCBL2. In contrast, WPK4 interacted with none of the CBLs...
  2. Batistic O, Sorek N, Schültke S, Yalovsky S, Kudla J. Dual fatty acyl modification determines the localization and plasma membrane targeting of CBL/CIPK Ca2+ signaling complexes in Arabidopsis. Plant Cell. 2008;20:1346-62 pubmed publisher
    ..Our findings demonstrate the importance of S-acylation for regulating the spatial accuracy of Ca2+-decoding proteins and suggest a novel mechanism that enables the functional specificity of calcium sensor/kinase complexes. ..
  3. Fuglsang A, Guo Y, Cuin T, Qiu Q, Song C, Kristiansen K, et al. Arabidopsis protein kinase PKS5 inhibits the plasma membrane H+ -ATPase by preventing interaction with 14-3-3 protein. Plant Cell. 2007;19:1617-34 pubmed
    ..We show that PKS5 interacts with the calcium binding protein SCaBP1 and that high external pH can trigger an increase in the concentration of cytosolic-free calcium...
  4. Yan J, Niu F, Liu W, Zhang H, Wang B, Lan W, et al. Arabidopsis CIPK14 positively regulates glucose response. Biochem Biophys Res Commun. 2014;450:1679-83 pubmed publisher
    ..1 and SnRK1.2 implicated in glucose signaling. This is the first report to demonstrate that CIPK also plays a role in glucose signaling. ..
  5. Xie C, Zhou X, Deng X, Guo Y. PKS5, a SNF1-related kinase, interacts with and phosphorylates NPR1, and modulates expression of WRKY38 and WRKY62. J Genet Genomics. 2010;37:359-69 pubmed publisher
    ..Our results suggest that PKS5 functions at the upstream of NPR1 and might mediate expression of WRKY38 and WRKY62 possibly by interacting with and phosphorylating NPR1. ..
  6. Du W, Lin H, Chen S, Wu Y, Zhang J, Fuglsang A, et al. Phosphorylation of SOS3-like calcium-binding proteins by their interacting SOS2-like protein kinases is a common regulatory mechanism in Arabidopsis. Plant Physiol. 2011;156:2235-43 pubmed publisher
    ..The phosphorylation site serine-216 at the C terminus of SCaBP1 by PKS24 was identified by liquid chromatography-quadrupole mass spectrometry analysis...
  7. Nozawa A, Koizumi N, Sano H. An Arabidopsis SNF1-related protein kinase, AtSR1, interacts with a calcium-binding protein, AtCBL2, of which transcripts respond to light. Plant Cell Physiol. 2001;42:976-81 pubmed
    ..and six calcineurin B like proteins of Arabidopsis and found that AtSR1 prominently interacts with one of them, AtCBL2, by yeast two-hybrid assay...
  8. Lan W, Lee S, Che Y, Jiang Y, Luan S. Mechanistic analysis of AKT1 regulation by the CBL-CIPK-PP2CA interactions. Mol Plant. 2011;4:527-36 pubmed publisher
    ..These findings provide further insights into the signaling network consisting of CBL-CIPK-PP2C interactions in the activation of the AKT1 channel. ..
  9. Eckert C, Offenborn J, Heinz T, Armarego Marriott T, Schültke S, Zhang C, et al. The vacuolar calcium sensors CBL2 and CBL3 affect seed size and embryonic development in Arabidopsis thaliana. Plant J. 2014;78:146-56 pubmed publisher
    ..In this study, we analyzed single and double mutants of the closely related tonoplast-localized calcium sensors CBL2 and CBL3, which show either reduction of function (rf) or complete loss of function (lf)...
  10. Shi J, Kim K, Ritz O, Albrecht V, Gupta R, Harter K, et al. Novel protein kinases associated with calcineurin B-like calcium sensors in Arabidopsis. Plant Cell. 1999;11:2393-405 pubmed
    ..These findings link a new type of Ca(2)+ sensors to a group of novel protein kinases, providing the molecular basis for a unique Ca(2)+ signaling machinery in plant cells. ..
  11. Tang R, Zhao F, Garcia V, Kleist T, Yang L, Zhang H, et al. Tonoplast CBL-CIPK calcium signaling network regulates magnesium homeostasis in Arabidopsis. Proc Natl Acad Sci U S A. 2015;112:3134-9 pubmed publisher
    ..In this study, we identified two calcineurin B-like (CBL) proteins, CBL2 and CBL3, as key regulators for plant growth under high-Mg conditions...
  12. Batistic O, Waadt R, Steinhorst L, Held K, Kudla J. CBL-mediated targeting of CIPKs facilitates the decoding of calcium signals emanating from distinct cellular stores. Plant J. 2010;61:211-22 pubmed publisher
  13. Lin H, Du W, Yang Y, Schumaker K, Guo Y. A calcium-independent activation of the Arabidopsis SOS2-like protein kinase24 by its interacting SOS3-like calcium binding protein1. Plant Physiol. 2014;164:2197-206 pubmed publisher
    ..Here, we report the calcium-independent activation of PKS24 by SCaBP1 and show that activation is dependent on interaction of PKS24 with the C-terminal tail of SCaBP1...
  14. Ok S, Cho J, Oh S, Choi M, Ma J, Shin J, et al. Calcineurin B-like 3 calcium sensor associates with and inhibits 5'-methylthioadenosine nucleosidase 2 in Arabidopsis. Plant Sci. 2015;238:228-40 pubmed publisher
    ..In the yeast two-hybrid system, AtMTAN1 interacted with multiple CBL members such as CBL2, CBL3 and CBL6, whereas AtMTAN2 associated exclusively with CBL3...
  15. Pandey G, Kanwar P, Singh A, Steinhorst L, Pandey A, Yadav A, et al. Calcineurin B-Like Protein-Interacting Protein Kinase CIPK21 Regulates Osmotic and Salt Stress Responses in Arabidopsis. Plant Physiol. 2015;169:780-92 pubmed publisher
    ..The calcium sensors CBL2 and CBL3 were found to physically interact with CIPK21 and target this kinase to the tonoplast...
  16. Steinhorst L, Mähs A, Ischebeck T, Zhang C, Zhang X, Arendt S, et al. Vacuolar CBL-CIPK12 Ca(2+)-sensor-kinase complexes are required for polarized pollen tube growth. Curr Biol. 2015;25:1475-82 pubmed publisher
    ..Here we identified the two closely related tonoplast-localized Ca(2+)-sensor proteins CBL2 and CBL3 as crucial regulators of vacuolar dynamics and polarized pollen tube growth...
  17. Akaboshi M, Hashimoto H, Ishida H, Saijo S, Koizumi N, Sato M, et al. The crystal structure of plant-specific calcium-binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14. J Mol Biol. 2008;377:246-57 pubmed publisher
    ..To better understand the functional role of the CBL-CIPK system, we determined the crystal structure of AtCBL2 in complex with the regulatory domain of AtCIPK14 at 1.2 A resolution...
  18. Lee S, Lan W, Kim B, Li L, Cheong Y, Pandey G, et al. A protein phosphorylation/dephosphorylation network regulates a plant potassium channel. Proc Natl Acad Sci U S A. 2007;104:15959-64 pubmed
  19. Albrecht V, Ritz O, Linder S, Harter K, Kudla J. The NAF domain defines a novel protein-protein interaction module conserved in Ca2+-regulated kinases. EMBO J. 2001;20:1051-63 pubmed
    ..Mutation of conserved residues either abolished or significantly diminished the affinity of AtCIPK1 for AtCBL2. Comprehensive two-hybrid screens with various AtCBLs identified 15 CIPKs as potential targets of CBL proteins...
  20. Liu L, Ren H, Chen L, Wang Y, Wu W. A protein kinase, calcineurin B-like protein-interacting protein Kinase9, interacts with calcium sensor calcineurin B-like Protein3 and regulates potassium homeostasis under low-potassium stress in Arabidopsis. Plant Physiol. 2013;161:266-77 pubmed publisher
    ..Furthermore, CBL2 and CBL3 were identified as upstream regulators of CIPK9...
  21. Zhou L, Fu Y, Yang Z. A genome-wide functional characterization of Arabidopsis regulatory calcium sensors in pollen tubes. J Integr Plant Biol. 2009;51:751-61 pubmed publisher
    ..CDPK32-GFP caused severe growth depolarization. CBL2-GFP and CBL3-GFP exhibited dynamic patterns of subcellular localization, including several endomembrane ..
  22. Guo Y, Halfter U, Ishitani M, Zhu J. Molecular characterization of functional domains in the protein kinase SOS2 that is required for plant salt tolerance. Plant Cell. 2001;13:1383-400 pubmed
    ..Combining the Thr(168)-to-Asp mutation with the autoinhibitory domain deletion created a superactive SOS2 kinase. These results provide insights into regulation of the kinase activities of SOS2 and the SOS2 family of protein kinases. ..