Gene Symbol: CBF2
Description: C-repeat/DRE binding factor 2
Alias: ATCBF2, C-repeat/DRE binding factor 2, DRE/CRT-BINDING PROTEIN, DRE/CRT-BINDING PROTEIN 1C, DREB1C, FREEZING TOLERANCE QTL 4, FTQ4, T30C3.12, C-repeat/DRE binding factor 2
Species: thale cress

Top Publications

  1. Alonso Blanco C, Gómez Mena C, Llorente F, Koornneef M, Salinas J, Martinez Zapater J. Genetic and molecular analyses of natural variation indicate CBF2 as a candidate gene for underlying a freezing tolerance quantitative trait locus in Arabidopsis. Plant Physiol. 2005;139:1304-12 pubmed
    ..FTQ4 colocated with the tandem repeated genes C-REPEAT BINDING FACTOR 1 (CBF1), CBF2, and CBF3, which encode transcriptional activators involved in the cold acclimation response...
  2. Gilmour S, Fowler S, Thomashow M. Arabidopsis transcriptional activators CBF1, CBF2, and CBF3 have matching functional activities. Plant Mol Biol. 2004;54:767-81 pubmed
    ..a small gene family encoding transcription factors known as CBF1, CBF2, and CBF3 (also referred to as DREB1b, DREB1c, and DREB1a, respectively) is rapidly induced followed by expression of CBF-targeted genes, the CBF regulon, which ..
  3. Fowler S, Cook D, Thomashow M. Low temperature induction of Arabidopsis CBF1, 2, and 3 is gated by the circadian clock. Plant Physiol. 2005;137:961-8 pubmed
    ..An analysis of CBF2 promoter-reporter gene fusions indicated that this control included transcriptional regulation...
  4. Novillo F, Medina J, Salinas J. Arabidopsis CBF1 and CBF3 have a different function than CBF2 in cold acclimation and define different gene classes in the CBF regulon. Proc Natl Acad Sci U S A. 2007;104:21002-7 pubmed
    ..factor (DREB1) proteins constitute a small family of Arabidopsis transcriptional activators (CBF1/DREB1B, CBF2/DREB1C, and CBF3/DREB1A) that play a prominent role in cold acclimation...
  5. Vergnolle C, Vaultier M, Taconnat L, Renou J, Kader J, Zachowski A, et al. The cold-induced early activation of phospholipase C and D pathways determines the response of two distinct clusters of genes in Arabidopsis cell suspensions. Plant Physiol. 2005;139:1217-33 pubmed
    ..The connection of these pathways with the CBF pathway, currently the most understood genetic system playing a role in cold acclimation, is discussed. ..
  6. Zhao C, Zhang Z, Xie S, Si T, Li Y, Zhu J. Mutational Evidence for the Critical Role of CBF Transcription Factors in Cold Acclimation in Arabidopsis. Plant Physiol. 2016;171:2744-59 pubmed publisher
    The three tandemly arranged CBF genes, CBF1, CBF2, and CBF3, are involved in cold acclimation...
  7. Sharabi Schwager M, Lers A, Samach A, Guy C, Porat R. Overexpression of the CBF2 transcriptional activator in Arabidopsis delays leaf senescence and extends plant longevity. J Exp Bot. 2010;61:261-73 pubmed publisher
    ..of plant frost tolerance, overexpression of the C-repeat/dehydration responsive element binding factor 2 (CBF2) gene in Arabidopsis delayed the onset of leaf senescence and extended the life span of the plants by approximately ..
  8. Sharabi Schwager M, Samach A, Porat R. Overexpression of the CBF2 transcriptional activator in Arabidopsis suppresses the responsiveness of leaf tissue to the stress hormone ethylene. Plant Biol (Stuttg). 2010;12:630-8 pubmed publisher
    ..We found that overexpression of CBF2 in Arabidopsis suppressed leaf tissue responsiveness to ethylene as compared with wild-type plants, as manifested ..
  9. Lin Y, Hwang S, Hsu P, Chiang Y, Huang C, Wang C, et al. Molecular population genetics and gene expression analysis of duplicated CBF genes of Arabidopsis thaliana. BMC Plant Biol. 2008;8:111 pubmed publisher
    ..Molecular population genetic analyses of these genes indicated that CBF2 has much reduced nucleotide diversity in the transcriptional unit and promoter, suggesting that CBF2 has been ..

More Information


  1. Hu P, Maiti T. A nonparametric mean-variance smoothing method to assess Arabidopsis cold stress transcriptional regulator CBF2 overexpression microarray data. PLoS ONE. 2011;6:e19640 pubmed publisher
    ..A real biological dataset, in which a cold stress transcription factor gene, CBF2, was overexpressed, has also been analyzed with the three methods...
  2. Shi Y, Huang J, Sun T, Wang X, Zhu C, Ai Y, et al. The precise regulation of different COR genes by individual CBF transcription factors in Arabidopsis thaliana. J Integr Plant Biol. 2017;59:118-133 pubmed publisher
    ..The C-repeat (CRT) motif (5'-CCGAC-3') was enriched in promoters of genes that were up-regulated by CBF2 and CBF3 but not in promoters of genes up-regulated by CBF1...
  3. Jia Y, Ding Y, Shi Y, Zhang X, Gong Z, Yang S. The cbfs triple mutants reveal the essential functions of CBFs in cold acclimation and allow the definition of CBF regulons in Arabidopsis. New Phytol. 2016;212:345-53 pubmed publisher
    ..In this study, we used CRISPR/Cas9 technology to mutate CBF1 or CBF1/CBF2 in a cbf3 T-DNA insertion mutant to generate cbf1,3 double and cbf1 cbf2 cbf3 (cbfs) triple mutants...
  4. Akhtar M, Jaiswal A, Taj G, Jaiswal J, Qureshi M, Singh N. DREB1/CBF transcription factors: their structure, function and role in abiotic stress tolerance in plants. J Genet. 2012;91:385-95 pubmed
    ..The recent progress related to genetic engineering of DREB1/CBF transcription factors in various crops and model plants is also summarized. ..
  5. Chan Z, Wang Y, Cao M, Gong Y, Mu Z, Wang H, et al. RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway. New Phytol. 2016;209:1527-39 pubmed publisher
    ..Chromatin immunoprecipitation results suggested that RDM4 is important for Pol II occupancy at the promoters of CBF2 and CBF3...
  6. Medina J, Bargues M, Terol J, Perez Alonso M, Salinas J. The Arabidopsis CBF gene family is composed of three genes encoding AP2 domain-containing proteins whose expression Is regulated by low temperature but not by abscisic acid or dehydration. Plant Physiol. 1999;119:463-70 pubmed
    ..These two genes, which we have named CBF2 and CBF3, also encode proteins containing AP2 DNA-binding motifs...
  7. Kidokoro S, Maruyama K, Nakashima K, Imura Y, Narusaka Y, Shinwari Z, et al. The phytochrome-interacting factor PIF7 negatively regulates DREB1 expression under circadian control in Arabidopsis. Plant Physiol. 2009;151:2046-57 pubmed publisher
    ..Promoter sequences of DREB1s contain six conserved motifs, boxes I to VI. We analyzed the promoter region of DREB1C using transgenic plants and found that box V with the G-box sequence negatively regulates DREB1C expression under ..
  8. Kang J, Zhang H, Sun T, Shi Y, Wang J, Zhang B, et al. Natural variation of C-repeat-binding factor (CBFs) genes is a major cause of divergence in freezing tolerance among a group of Arabidopsis thaliana populations along the Yangtze River in China. New Phytol. 2013;199:1069-80 pubmed publisher
    ..major locus harboring three cold-responsive transcription factor genes CBF1-3, and found a nucleotide insertion in CBF2 in all populations except SXcgx, which generated a dysfunctional CBF2 protein...
  9. Gehan M, Park S, Gilmour S, An C, Lee C, Thomashow M. Natural variation in the C-repeat binding factor cold response pathway correlates with local adaptation of Arabidopsis ecotypes. Plant J. 2015;84:682-93 pubmed publisher
    ..The CBF locus includes three genes - CBF1, CBF2 and CBF3 - that are induced by low temperature and encode transcription factors that regulate a group of more than ..
  10. Seo E, Lee H, Jeon J, Park H, Kim J, Noh Y, et al. Crosstalk between cold response and flowering in Arabidopsis is mediated through the flowering-time gene SOC1 and its upstream negative regulator FLC. Plant Cell. 2009;21:3185-97 pubmed publisher
  11. Shinwari Z, Nakashima K, Miura S, Kasuga M, Seki M, Yamaguchi Shinozaki K, et al. An Arabidopsis gene family encoding DRE/CRT binding proteins involved in low-temperature-responsive gene expression. Biochem Biophys Res Commun. 1998;250:161-70 pubmed
    ..library with the cDNA fragment of DREB1A as a probe and isolated DREB1A and 2 related genes, DREB1B (= CBF1) and DREB1C. These were arrayed in the order B, A, C in an 8.7 kb region of Arabidopsis chromosome 4...