Gene Symbol: CBF1
Description: C-repeat/DRE binding factor 1
Alias: ATCBF1, C-repeat/DRE binding factor 1, DRE BINDING PROTEIN 1B, DREB1B, T30C3.11, TRANSCRIPTIONAL ACTIVATOR CBF1, C-repeat/DRE binding factor 1
Species: thale cress

Top Publications

  1. Fowler S, Cook D, Thomashow M. Low temperature induction of Arabidopsis CBF1, 2, and 3 is gated by the circadian clock. Plant Physiol. 2005;137:961-8 pubmed
    ..thaliana) plants to low temperature results in rapid induction of CBF1, 2, and 3 (CBF1-3; also known as DREB1B, C, and A, respectively), which encode transcriptional activators that induce expression of a battery of genes ..
  2. Novillo F, Medina J, Salinas J. Arabidopsis CBF1 and CBF3 have a different function than CBF2 in cold acclimation and define different gene classes in the CBF regulon. Proc Natl Acad Sci U S A. 2007;104:21002-7 pubmed
    ..element-binding factor (DREB1) proteins constitute a small family of Arabidopsis transcriptional activators (CBF1/DREB1B, CBF2/DREB1C, and CBF3/DREB1A) that play a prominent role in cold acclimation...
  3. Jaglo Ottosen K, Gilmour S, Zarka D, Schabenberger O, Thomashow M. Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance. Science. 1998;280:104-6 pubmed
    ..Increased expression of Arabidopsis CBF1, a transcriptional activator that binds to the CRT/DRE sequence, induced COR gene expression and increased the ..
  4. Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi Shinozaki K, et al. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell. 1998;10:1391-406 pubmed
    ..These results indicate that two independent families of DREB proteins, DREB1 and DREB2, function as trans-acting factors in two separate signal transduction pathways under low-temperature and dehydration conditions, respectively. ..
  5. Pino M, Skinner J, Jeknic Z, Hayes P, Soeldner A, Thomashow M, et al. Ectopic AtCBF1 over-expression enhances freezing tolerance and induces cold acclimation-associated physiological modifications in potato. Plant Cell Environ. 2008;31:393-406 pubmed publisher
    ..Relative to wild-type plants, ectopic AtCBF1 over-expression induced expression of COR genes without a cold stimulus in both species, and imparted a ..
  6. Zhao C, Zhang Z, Xie S, Si T, Li Y, Zhu J. Mutational Evidence for the Critical Role of CBF Transcription Factors in Cold Acclimation in Arabidopsis. Plant Physiol. 2016;171:2744-59 pubmed publisher
    The three tandemly arranged CBF genes, CBF1, CBF2, and CBF3, are involved in cold acclimation...
  7. Stockinger E, Mao Y, Regier M, Triezenberg S, Thomashow M. Transcriptional adaptor and histone acetyltransferase proteins in Arabidopsis and their interactions with CBF1, a transcriptional activator involved in cold-regulated gene expression. Nucleic Acids Res. 2001;29:1524-33 pubmed
    ..Expression of the CBF1 proteins in yeast activates reporter genes carrying a minimal promoter with the CRT/DRE as an upstream regulatory ..
  8. Vergnolle C, Vaultier M, Taconnat L, Renou J, Kader J, Zachowski A, et al. The cold-induced early activation of phospholipase C and D pathways determines the response of two distinct clusters of genes in Arabidopsis cell suspensions. Plant Physiol. 2005;139:1217-33 pubmed
    ..The connection of these pathways with the CBF pathway, currently the most understood genetic system playing a role in cold acclimation, is discussed. ..
  9. Hu Y, Jiang L, Wang F, Yu D. Jasmonate regulates the inducer of cbf expression-C-repeat binding factor/DRE binding factor1 cascade and freezing tolerance in Arabidopsis. Plant Cell. 2013;25:2907-24 pubmed publisher
    ..Taken together, our study provides evidence that jasmonate functions as a critical upstream signal of the ICE-CBF/DREB1 pathway to positively regulate Arabidopsis freezing tolerance...

More Information


  1. Yang S, Wang S, Liu X, Yu Y, Yue L, Wang X, et al. Four divergent Arabidopsis ethylene-responsive element-binding factor domains bind to a target DNA motif with a universal CG step core recognition and different flanking bases preference. FEBS J. 2009;276:7177-86 pubmed publisher
    ..AtERF1, AtERF4, AtEBP and CBF1 are members from different phylogenetic subgroups within the family...
  2. Chow B, Sanchez S, Breton G, Pruneda Paz J, Krogan N, Kay S. Transcriptional regulation of LUX by CBF1 mediates cold input to the circadian clock in Arabidopsis. Curr Biol. 2014;24:1518-24 pubmed publisher
    ..report the well-characterized cold-inducible C-repeat (CRT)/drought-responsive element (DRE) binding factor CBF1/DREB1b is a transcriptional regulator of LUX...
  3. Li C, Su R, Cheng C, You S, Hsieh T, Chao T, et al. Tomato RAV transcription factor is a pivotal modulator involved in the AP2/EREBP-mediated defense pathway. Plant Physiol. 2011;156:213-27 pubmed publisher
    ..profile in transgenic tomato (Solanum lycopersicum) overexpressing Arabidopsis (Arabidopsis thaliana) CBF1 (AtCBF1), which confers tolerance to BW...
  4. Lin Y, Hwang S, Hsu P, Chiang Y, Huang C, Wang C, et al. Molecular population genetics and gene expression analysis of duplicated CBF genes of Arabidopsis thaliana. BMC Plant Biol. 2008;8:111 pubmed publisher
    ..We explored patterns of genetic variations of CBF1, -2, and -3 from 34 accessions of Arabidopsis thaliana...
  5. Singh S, Rathore M, Goyary D, Singh R, Anandhan S, Sharma D, et al. Induced ectopic expression of At-CBF1 in marker-free transgenic tomatoes confers enhanced chilling tolerance. Plant Cell Rep. 2011;30:1019-28 pubmed publisher
    In an attempt to improve chilling stress tolerance, an Arabidopsis C-repeat binding factor 1 (At-CBF1) gene driven by the inducible promoter RD29A was co-transferred into tomato var. Shalimar...
  6. Achard P, Gong F, Cheminant S, Alioua M, Hedden P, Genschik P. The cold-inducible CBF1 factor-dependent signaling pathway modulates the accumulation of the growth-repressing DELLA proteins via its effect on gibberellin metabolism. Plant Cell. 2008;20:2117-29 pubmed publisher
    ..The C-repeat/drought-responsive element binding factor CBF1/DREB1b gene encodes a transcriptional activator transiently induced by cold that controls the expression of a set of ..
  7. Shi Y, Huang J, Sun T, Wang X, Zhu C, Ai Y, et al. The precise regulation of different COR genes by individual CBF transcription factors in Arabidopsis thaliana. J Integr Plant Biol. 2017;59:118-133 pubmed publisher
    The transcription factors CBF1/2/3 are reported to play a dominant role in the cold responsive network of Arabidopsis by directly regulating the expression levels of cold responsive (COR) genes...
  8. Stockinger E, Gilmour S, Thomashow M. Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc Natl Acad Sci U S A. 1997;94:1035-40 pubmed
    ..Here we report the isolation of an Arabidopsis thaliana cDNA that encodes a C-repeat/DRE binding factor, CBF1 (C-repeat/DRE Binding Factor 1)...
  9. Yang J, Wang R, Meng J, Bi Y, Xu P, Guo F, et al. Overexpression of Arabidopsis CBF1 gene in transgenic tobacco alleviates photoinhibition of PSII and PSI during chilling stress under low irradiance. J Plant Physiol. 2010;167:534-9 pubmed publisher
    A known arabidopsis cDNA clone, the CRT/DRE binding factor 1 (CBF1), was isolated and introduced into tobacco plants...
  10. Akhtar M, Jaiswal A, Taj G, Jaiswal J, Qureshi M, Singh N. DREB1/CBF transcription factors: their structure, function and role in abiotic stress tolerance in plants. J Genet. 2012;91:385-95 pubmed
    ..The recent progress related to genetic engineering of DREB1/CBF transcription factors in various crops and model plants is also summarized. ..
  11. Canella D, Gilmour S, Kuhn L, Thomashow M. DNA binding by the Arabidopsis CBF1 transcription factor requires the PKKP/RAGRxKFxETRHP signature sequence. Biochim Biophys Acta. 2010;1799:454-62 pubmed publisher
    ..Here we show that the PKKPAGR sequence of AtCBF1 is essential for its transcriptional activity...
  12. Shinwari Z, Nakashima K, Miura S, Kasuga M, Seki M, Yamaguchi Shinozaki K, et al. An Arabidopsis gene family encoding DRE/CRT binding proteins involved in low-temperature-responsive gene expression. Biochem Biophys Res Commun. 1998;250:161-70 pubmed
    ..genomic DNA library with the cDNA fragment of DREB1A as a probe and isolated DREB1A and 2 related genes, DREB1B (= CBF1) and DREB1C. These were arrayed in the order B, A, C in an 8.7 kb region of Arabidopsis chromosome 4...
  13. Jia Y, Ding Y, Shi Y, Zhang X, Gong Z, Yang S. The cbfs triple mutants reveal the essential functions of CBFs in cold acclimation and allow the definition of CBF regulons in Arabidopsis. New Phytol. 2016;212:345-53 pubmed publisher
    ..In this study, we used CRISPR/Cas9 technology to mutate CBF1 or CBF1/CBF2 in a cbf3 T-DNA insertion mutant to generate cbf1,3 double and cbf1 cbf2 cbf3 (cbfs) triple mutants...
  14. Liu Z, Jia Y, Ding Y, Shi Y, Li Z, Guo Y, et al. Plasma Membrane CRPK1-Mediated Phosphorylation of 14-3-3 Proteins Induces Their Nuclear Import to Fine-Tune CBF Signaling during Cold Response. Mol Cell. 2017;66:117-128.e5 pubmed publisher
    ..Thus, our study reveals that the CRPK1-14-3-3 module transduces the cold signal from the plasma membrane to the nucleus to modulate CBF stability, which ensures a faithfully adjusted response to cold stress of plants. ..
  15. Gehan M, Park S, Gilmour S, An C, Lee C, Thomashow M. Natural variation in the C-repeat binding factor cold response pathway correlates with local adaptation of Arabidopsis ecotypes. Plant J. 2015;84:682-93 pubmed publisher
    ..The CBF locus includes three genes - CBF1, CBF2 and CBF3 - that are induced by low temperature and encode transcription factors that regulate a group of more ..
  16. Kang J, Zhang H, Sun T, Shi Y, Wang J, Zhang B, et al. Natural variation of C-repeat-binding factor (CBFs) genes is a major cause of divergence in freezing tolerance among a group of Arabidopsis thaliana populations along the Yangtze River in China. New Phytol. 2013;199:1069-80 pubmed publisher
    ..We identified a major locus harboring three cold-responsive transcription factor genes CBF1-3, and found a nucleotide insertion in CBF2 in all populations except SXcgx, which generated a dysfunctional CBF2 ..
  17. Mao Y, Pavangadkar K, Thomashow M, Triezenberg S. Physical and functional interactions of Arabidopsis ADA2 transcriptional coactivator proteins with the acetyltransferase GCN5 and with the cold-induced transcription factor CBF1. Biochim Biophys Acta. 2006;1759:69-79 pubmed
    ..coactivator proteins in the stimulation of cold-regulated gene expression by the transcriptional activator protein CBF1. Surprisingly, protein interaction studies demonstrate that the DNA-binding domain of CBF1 (and of a related ..
  18. Seo E, Lee H, Jeon J, Park H, Kim J, Noh Y, et al. Crosstalk between cold response and flowering in Arabidopsis is mediated through the flowering-time gene SOC1 and its upstream negative regulator FLC. Plant Cell. 2009;21:3185-97 pubmed publisher
  19. Benedict C, Skinner J, Meng R, Chang Y, Bhalerao R, Huner N, et al. The CBF1-dependent low temperature signalling pathway, regulon and increase in freeze tolerance are conserved in Populus spp. Plant Cell Environ. 2006;29:1259-72 pubmed
    ..changes in phenotype and transcript profile of transgenic Populus constitutively expressing CBF1 from Arabidopsis (AtCBF1)...