CAO

Summary

Gene Symbol: CAO
Description: chloroplast signal recognition particle component (CAO)
Alias: CHAOS, CHLOROPLAST SIGNAL RECOGNITION PARTICLE 43, CPSRP43, T30B22.25, chloroplast signal recognition particle component (CAO)
Species: thale cress

Top Publications

  1. Tu C, Schuenemann D, Hoffman N. Chloroplast FtsY, chloroplast signal recognition particle, and GTP are required to reconstitute the soluble phase of light-harvesting chlorophyll protein transport into thylakoid membranes. J Biol Chem. 1999;274:27219-24 pubmed
    ..We have examined the subunit stoichiometry of cpSRP and find that it is trimeric and composed of two subunits of cpSRP43/subunit of cpSRP54...
  2. Ouyang M, Li X, Ma J, Chi W, Xiao J, Zou M, et al. LTD is a protein required for sorting light-harvesting chlorophyll-binding proteins to the chloroplast SRP pathway. Nat Commun. 2011;2:277 pubmed publisher
    ..Our study shows that LTD is essential for the import of light-harvesting chlorophyll-binding proteins and subsequent routing of these proteins to the chloroplast SRP-dependent pathway. ..
  3. Bals T, Dünschede B, Funke S, Schünemann D. Interplay between the cpSRP pathway components, the substrate LHCP and the translocase Alb3: an in vivo and in vitro study. FEBS Lett. 2010;584:4138-44 pubmed publisher
    ..We propose that cpSRP43 is crucial for the binding of LHCP-loaded cpSRP and cpFtsY to Alb3...
  4. Gao F, Kight A, Henderson R, Jayanthi S, Patel P, Murchison M, et al. Regulation of Structural Dynamics within a Signal Recognition Particle Promotes Binding of Protein Targeting Substrates. J Biol Chem. 2015;290:15462-74 pubmed publisher
    ..A multidomain chloroplast-specific subunit of the SRP, cpSRP43, is proposed to take on the role of coordinating the sequence of targeting events...
  5. Kathir K, Rajalingam D, Sivaraja V, Kight A, Goforth R, Yu C, et al. Assembly of chloroplast signal recognition particle involves structural rearrangement in cpSRP43. J Mol Biol. 2008;381:49-60 pubmed publisher
    ..as a heterodimer composed of a conserved 54-kDa guanosine triphosphatase (cpSRP54) and a unique 43-kDa subunit (cpSRP43)...
  6. Klenell M, Morita S, Tiemblo Olmo M, Mühlenbock P, Karpinski S, Karpinska B. Involvement of the chloroplast signal recognition particle cpSRP43 in acclimation to conditions promoting photooxidative stress in Arabidopsis. Plant Cell Physiol. 2005;46:118-29 pubmed
    In this study, we have investigated the role of the CAO gene (coding for the chloroplast recognition particle cpSRP43) in the protection against and acclimation to environmental conditions that promote photooxidative stress...
  7. Falk S, Ravaud S, Koch J, Sinning I. The C terminus of the Alb3 membrane insertase recruits cpSRP43 to the thylakoid membrane. J Biol Chem. 2010;285:5954-62 pubmed publisher
    ..of chromodomain interaction motifs in histone tails are identified in A3CT that are essential for the Alb3-cpSRP43 interaction. They are absent in the C-terminal domain of Alb4, which therefore does not interact with cpSRP43...
  8. Goforth R, Peterson E, Yuan J, Moore M, Kight A, Lohse M, et al. Regulation of the GTPase cycle in post-translational signal recognition particle-based protein targeting involves cpSRP43. J Biol Chem. 2004;279:43077-84 pubmed
    ..signal recognition particle consists of a conserved 54-kDa GTPase and a novel 43-kDa chromodomain protein (cpSRP43) that together bind light-harvesting chlorophyll a/b-binding protein (LHCP) to form a soluble targeting complex ..
  9. Trösch R, Töpel M, Flores Pérez Ã, Jarvis P. Genetic and Physical Interaction Studies Reveal Functional Similarities between ALBINO3 and ALBINO4 in Arabidopsis. Plant Physiol. 2015;169:1292-306 pubmed publisher
    ..been established mainly for light-harvesting complex proteins, which first interact with the unique chloroplast cpSRP43 component and then are delivered to the ALB3 integrase by a GTP-dependent cpSRP-cpFtsY interaction...

More Information

Publications21

  1. Stengel K, Holdermann I, Cain P, Robinson C, Wild K, Sinning I. Structural basis for specific substrate recognition by the chloroplast signal recognition particle protein cpSRP43. Science. 2008;321:253-6 pubmed publisher
    ..and posttranslationally targeted to the thylakoid membrane by cpSRP, a heterodimer formed by cpSRP54 and cpSRP43. We present the 1...
  2. Lewis N, Marty N, Kathir K, Rajalingam D, Kight A, Daily A, et al. A dynamic cpSRP43-Albino3 interaction mediates translocase regulation of chloroplast signal recognition particle (cpSRP)-targeting components. J Biol Chem. 2010;285:34220-30 pubmed publisher
    ..a combination of studies that explore the binding interface and functional role of a previously identified cpSRP43-Alb3 interaction...
  3. Klimyuk V, Persello Cartieaux F, Havaux M, Contard David P, Schuenemann D, Meiherhoff K, et al. A chromodomain protein encoded by the arabidopsis CAO gene is a plant-specific component of the chloroplast signal recognition particle pathway that is involved in LHCP targeting. Plant Cell. 1999;11:87-99 pubmed
    ..In chloroplasts, the homolog of SRP54 was found to bind a 43-kD protein (cpSRP43) rather than to an RNA. We cloned the CAO gene, which encodes a protein identified as Arabidopsis cpSRP43...
  4. Holdermann I, Meyer N, Round A, Wild K, Sattler M, Sinning I. Chromodomains read the arginine code of post-translational targeting. Nat Struct Mol Biol. 2012;19:260-3 pubmed publisher
    ..Arabidopsis thaliana, with the cpSRP54 tail comprising an arginine-rich motif bound to the second chromodomain of cpSRP43. A twinned aromatic cage reads out two neighboring nonmethylated arginines and adapts chromodomains to a non-..
  5. Jaru Ampornpan P, Liang F, Nisthal A, Nguyen T, Wang P, Shen K, et al. Mechanism of an ATP-independent protein disaggregase: II. distinct molecular interactions drive multiple steps during aggregate disassembly. J Biol Chem. 2013;288:13431-45 pubmed publisher
    ..large protein aggregates is provided by the 38-kDa subunit of the chloroplast signal recognition particle (cpSRP43), which uses binding energy with its substrate proteins to drive disaggregation...
  6. Tzvetkova Chevolleau T, Hutin C, Noël L, Goforth R, Carde J, Caffarri S, et al. Canonical signal recognition particle components can be bypassed for posttranslational protein targeting in chloroplasts. Plant Cell. 2007;19:1635-48 pubmed
    ..Using a mutant form of cpSRP43 that is unable to assemble with cpSRP54, we complemented the cpSRP43-deficient mutant and found that this subunit ..
  7. Dünschede B, Bals T, Funke S, Schünemann D. Interaction studies between the chloroplast signal recognition particle subunit cpSRP43 and the full-length translocase Alb3 reveal a membrane-embedded binding region in Alb3 protein. J Biol Chem. 2011;286:35187-95 pubmed publisher
    ..Here, we analyzed the interaction sites of full-length Alb3 with the cpSRP pathway component cpSRP43 by using in vitro and in vivo studies...
  8. Nguyen T, Jaru Ampornpan P, Lam V, Cao P, Piszkiewicz S, Hess S, et al. Mechanism of an ATP-independent protein disaggregase: I. structure of a membrane protein aggregate reveals a mechanism of recognition by its chaperone. J Biol Chem. 2013;288:13420-30 pubmed publisher
    A novel chaperone, cpSRP43, recognizes and disassembles the aggregates formed by its client proteins. The client proteins of cpSRP43 form stable disc-shaped aggregates with the chaperone recognition motif displayed onthe surface...
  9. Moore M, Goforth R, Mori H, Henry R. Functional interaction of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids: substrate not required. J Cell Biol. 2003;162:1245-54 pubmed
    ..Surprisingly, neither substrate nor cpSRP43, a component of cpSRP, was necessary to form a complex with ALB3...
  10. Horn A, Hennig J, Ahmed Y, Stier G, Wild K, Sattler M, et al. Structural basis for cpSRP43 chromodomain selectivity and dynamics in Alb3 insertase interaction. Nat Commun. 2015;6:8875 pubmed publisher
    ..in chloroplasts to the Alb3 insertase occurs post-translationally via a soluble transit complex including the cpSRP43/cpSRP54 heterodimer (cpSRP)...
  11. Dünschede B, Träger C, Schröder C, Ziehe D, Walter B, Funke S, et al. Chloroplast SRP54 Was Recruited for Posttranslational Protein Transport via Complex Formation with Chloroplast SRP43 during Land Plant Evolution. J Biol Chem. 2015;290:13104-14 pubmed publisher
    ..an SRP RNA, an essential SRP component in bacteria, but forms a stable heterodimer with the chloroplast-specific cpSRP43. This heterodimeric cpSRP recognizes LHCP and delivers it to the thylakoid membrane whereby cpSRP43 plays a ..
  12. Liang F, Kroon G, McAvoy C, Chi C, Wright P, Shan S. Conformational dynamics of a membrane protein chaperone enables spatially regulated substrate capture and release. Proc Natl Acad Sci U S A. 2016;113:E1615-24 pubmed publisher
    ..Here we define the chaperone cycle for cpSRP43, which protects the largest family of membrane proteins, the light harvesting chlorophyll a/b-binding proteins (..