AGB1

Summary

Gene Symbol: AGB1
Description: GTP binding protein beta 1
Alias: ARABIDOPSIS G-PROTEIN BETA SUBUNIT, ATAGB1, ELK4, ERECTA-LIKE 4, GTP binding protein beta 1, T4L20.40, T4L20_40, GTP binding protein beta 1
Species: thale cress
Products:     AGB1

Top Publications

  1. Pandey S, Monshausen G, Ding L, Assmann S. Regulation of root-wave response by extra large and conventional G proteins in Arabidopsis thaliana. Plant J. 2008;55:311-22 pubmed publisher
    ..In this paper we show that one of the three XLG genes, XLG3, and the Gbeta subunit (AGB1) of the Arabidopsis G-protein heterotrimer are specifically involved in the regulation of a subset of root ..
  2. Trusov Y, Zhang W, Assmann S, Botella J. Ggamma1 + Ggamma2 not equal to Gbeta: heterotrimeric G protein Ggamma-deficient mutants do not recapitulate all phenotypes of Gbeta-deficient mutants. Plant Physiol. 2008;147:636-49 pubmed publisher
    ..The Arabidopsis (Arabidopsis thaliana) genome contains one Galpha (GPA1), one Gbeta (AGB1), and two Ggamma subunit (AGG1 and AGG2) genes...
  3. Fan L, Zhang W, Chen J, Taylor J, Jones A, Assmann S. Abscisic acid regulation of guard-cell K+ and anion channels in Gbeta- and RGS-deficient Arabidopsis lines. Proc Natl Acad Sci U S A. 2008;105:8476-81 pubmed publisher
    ..We evaluated plants harboring mutations in the sole Arabidopsis Galpha (AtGPA1), Gbeta (AGB1), and Regulator of G protein Signaling (AtRGS1) genes for impacts on ion channel regulation...
  4. Ishikawa A. The Arabidopsis G-protein beta-subunit is required for defense response against Agrobacterium tumefaciens. Biosci Biotechnol Biochem. 2009;73:47-52 pubmed
    ..In this study, we found that in agb1-2 (AGB1 null mutation) mutants, ROS production triggered by flg22 or elf18 was significantly reduced and that elf18-..
  5. Kansup J, Tsugama D, Liu S, Takano T. The Arabidopsis adaptor protein AP-3? interacts with the G-protein ? subunit AGB1 and is involved in abscisic acid regulation of germination and post-germination development. J Exp Bot. 2013;64:5611-21 pubmed publisher
    ..A yeast two-hybrid screen was performed to identify interacting partners of the Arabidopsis G-protein ? subunit AGB1. One of the identified AGB1-interacting proteins is the Arabidopsis adaptor protein AP-3µ...
  6. Pandey S, Chen J, Jones A, Assmann S. G-protein complex mutants are hypersensitive to abscisic acid regulation of germination and postgermination development. Plant Physiol. 2006;141:243-56 pubmed
    ..To assess new roles for the Arabidopsis (Arabidopsis thaliana) Galpha subunit (GPA1), the Gbeta subunit (AGB1), and the candidate G-protein-coupled receptor (GCR1) in ABA signaling during germination and early seedling ..
  7. Tsugama D, Liu H, Liu S, Takano T. Arabidopsis heterotrimeric G protein ? subunit interacts with a plasma membrane 2C-type protein phosphatase, PP2C52. Biochim Biophys Acta. 2012;1823:2254-60 pubmed publisher
    ..a yeast two-hybrid screen that a putative myristoylated 2C-type protein phosphatase, PP2C52, is an Arabidopsis G? (AGB1)-interacting partner...
  8. Mudgil Y, Uhrig J, Zhou J, Temple B, Jiang K, Jones A. Arabidopsis N-MYC DOWNREGULATED-LIKE1, a positive regulator of auxin transport in a G protein-mediated pathway. Plant Cell. 2009;21:3591-609 pubmed publisher
    ..A null mutation in either the single beta (AGB1) or the two gamma (AGG1 and AGG2) subunits confers phenotypes that disrupt the proper architecture of Arabidopsis ..
  9. Zhang L, Hu G, Cheng Y, Huang J. Heterotrimeric G protein alpha and beta subunits antagonistically modulate stomatal density in Arabidopsis thaliana. Dev Biol. 2008;324:68-75 pubmed publisher
    ..In contrast, stomatal density increased in the null mutant of the G-protein beta-subunit (AGB1) but decreased in transgenic lines that overexpressed AGB1...

More Information

Publications52

  1. Chakravorty D, Trusov Y, Zhang W, Acharya B, Sheahan M, McCurdy D, et al. An atypical heterotrimeric G-protein ?-subunit is involved in guard cell K?-channel regulation and morphological development in Arabidopsis thaliana. Plant J. 2011;67:840-51 pubmed publisher
    ..The discovery of this highly atypical subunit reinforces the emerging notion that plant and animal G-proteins have distinct as well as shared evolutionary pathways. ..
  2. Friedman E, Wang H, Jiang K, Perovic I, Deshpande A, Pochapsky T, et al. Acireductone dioxygenase 1 (ARD1) is an effector of the heterotrimeric G protein beta subunit in Arabidopsis. J Biol Chem. 2011;286:30107-18 pubmed publisher
    ..Arabidopsis thaliana contains one G?, one G? (AGB1), and at least three G? subunits, allowing it to form three versions of the heterotrimer...
  3. Tsugama D, Liu S, Takano T. Arabidopsis heterotrimeric G protein ? subunit, AGB1, regulates brassinosteroid signalling independently of BZR1. J Exp Bot. 2013;64:3213-23 pubmed publisher
    The Arabidopsis thaliana heterotrimeric G protein ? subunit, AGB1, is involved in both abscisic acid (ABA) signalling and brassinosteroid (BR) signalling, but it is unclear how AGB1 regulates these signalling pathways...
  4. Trusov Y, Rookes J, Chakravorty D, Armour D, Schenk P, Botella J. Heterotrimeric G proteins facilitate Arabidopsis resistance to necrotrophic pathogens and are involved in jasmonate signaling. Plant Physiol. 2006;140:210-20 pubmed
    ..We hypothesize that Gbetagamma acts as a direct or indirect enhancer of the jasmonate signaling pathway in plants. ..
  5. Kobayashi S, Tsugama D, Liu S, Takano T. A U-Box E3 ubiquitin ligase, PUB20, interacts with the Arabidopsis G-protein ? subunit, AGB1. PLoS ONE. 2012;7:e49207 pubmed publisher
    ..alternatively called AtCMPG1) was identified as a possible interactor of the Arabidopsis G-protein ? subunit, AGB1, by yeast two-hybrid screening...
  6. Mason M, Botella J. Completing the heterotrimer: isolation and characterization of an Arabidopsis thaliana G protein gamma-subunit cDNA. Proc Natl Acad Sci U S A. 2000;97:14784-8 pubmed
    ..that AGG1 is a single-copy gene in Arabidopsis with a similar expression pattern to the Arabidopsis beta-subunit, AGB1 [Weiss, C. A., Garnaat, C. W., Mukai, K., Hu, Y. & Ma, H. (1994) Proc. Natl. Acad. Sci. USA 91, 9554-9558]...
  7. Tsugama D, Liu S, Takano T. A bZIP protein, VIP1, interacts with Arabidopsis heterotrimeric G protein ? subunit, AGB1. Plant Physiol Biochem. 2013;71:240-6 pubmed publisher
    ..By a yeast two-hybrid screen, we identified a bZIP protein, VIP1, as an Arabidopsis thaliana G? (AGB1)-interacting partner...
  8. Lease K, Wen J, Li J, Doke J, Liscum E, Walker J. A mutant Arabidopsis heterotrimeric G-protein beta subunit affects leaf, flower, and fruit development. Plant Cell. 2001;13:2631-41 pubmed
    ..We positionally cloned ELK4 and determined that it encodes AGB1, a putative heterotrimeric G-protein beta subunit. Therefore, elk4 was renamed agb1...
  9. Wang S, Assmann S, Fedoroff N. Characterization of the Arabidopsis heterotrimeric G protein. J Biol Chem. 2008;283:13913-22 pubmed publisher
  10. Anderson D, Botella J. Expression analysis and subcellular localization of the Arabidopsis thaliana G-protein beta-subunit AGB1. Plant Cell Rep. 2007;26:1469-80 pubmed
    ..subcellular localization to this multifunctional signalling capacity for the Arabidopsis thaliana Gbeta subunit, AGB1. Analysis of AGB1 promoter::beta-glucuronidase (GUS) fusions in germinating seeds, seedlings, and flowering plants ..
  11. Trusov Y, Sewelam N, Rookes J, Kunkel M, Nowak E, Schenk P, et al. Heterotrimeric G proteins-mediated resistance to necrotrophic pathogens includes mechanisms independent of salicylic acid-, jasmonic acid/ethylene- and abscisic acid-mediated defense signaling. Plant J. 2009;58:69-81 pubmed publisher
    ..mechanisms involving heterotrimeric G proteins, we analyzed the effects of the G? (subunit deficiency in the mutant agb1-2 on pathogenesis-related gene expression, as well as the genetic interaction between agb1-2 and a number of ..
  12. Mason M, Botella J. Isolation of a novel G-protein gamma-subunit from Arabidopsis thaliana and its interaction with Gbeta. Biochim Biophys Acta. 2001;1520:147-53 pubmed
    ..A strong interaction between AGG2 and both the tobacco (TGB1) and Arabidopsis (AGB1) beta-subunits was observed in vivo using the yeast two-hybrid system...
  13. Wang S, Narendra S, Fedoroff N. Heterotrimeric G protein signaling in the Arabidopsis unfolded protein response. Proc Natl Acad Sci U S A. 2007;104:3817-22 pubmed
    ..Seedlings of homozygous agb1-2 (Gbeta-null mutation) mutant plants are markedly more resistant to growth inhibition by the protein glycosylation ..
  14. Urano D, Phan N, Jones J, Yang J, Huang J, GRIGSTON J, et al. Endocytosis of the seven-transmembrane RGS1 protein activates G-protein-coupled signalling in Arabidopsis. Nat Cell Biol. 2012;14:1079-88 pubmed publisher
    ..These findings reveal how different organisms rearrange a regulatory system to result in opposite outcomes using similar phosphorylation-dependent endocytosis mechanisms. ..
  15. Pandey S, Wang R, Wilson L, Li S, Zhao Z, Gookin T, et al. Boolean modeling of transcriptome data reveals novel modes of heterotrimeric G-protein action. Mol Syst Biol. 2010;6:372 pubmed publisher
    ..Our method holds significant promise for analyzing analogous 'switch-like' signal transduction events in any organism...
  16. Yu Y, Assmann S. The heterotrimeric G-protein β subunit, AGB1, plays multiple roles in the Arabidopsis salinity response. Plant Cell Environ. 2015;38:2143-56 pubmed publisher
    ..The knockout mutant of the Arabidopsis heterotrimeric G-protein Gβ subunit, agb1, is hypersensitive to salt, exhibiting a leaf bleaching phenotype...
  17. Heo J, Sung S, Assmann S. Ca2+-dependent GTPase, extra-large G protein 2 (XLG2), promotes activation of DNA-binding protein related to vernalization 1 (RTV1), leading to activation of floral integrator genes and early flowering in Arabidopsis. J Biol Chem. 2012;287:8242-53 pubmed publisher
    ..Thus, a Ca(2+)-dependent G protein, XLG2, promotes RTV1 DNA binding activity for a subset of floral integrator genes and contributes to floral transition. ..
  18. Booker K, Schwarz J, Garrett M, Jones A. Glucose attenuation of auxin-mediated bimodality in lateral root formation is partly coupled by the heterotrimeric G protein complex. PLoS ONE. 2010;5: pubmed publisher
    ..The heterotrimeric G protein complex in Arabidopsis thaliana, defined as containing alpha (AtGPA1), beta (AGB1), and gamma (AGG) subunits and a GTPase accelerating protein called Regulator of G Signaling 1 protein (AtRGS1), ..
  19. Maeda K, Houjyou Y, Komatsu T, Hori H, Kodaira T, Ishikawa A. AGB1 and PMR5 contribute to PEN2-mediated preinvasion resistance to Magnaporthe oryzae in Arabidopsis thaliana. Mol Plant Microbe Interact. 2009;22:1331-40 pubmed publisher
    ..oryzae. Double mutant analysis indicated that AGB1 and PMR5 contribute to PEN2-mediated preinvasion resistance to M. oryzae in A...
  20. Lorek J, Griebel T, Jones A, Kuhn H, Panstruga R. The role of Arabidopsis heterotrimeric G-protein subunits in MLO2 function and MAMP-triggered immunity. Mol Plant Microbe Interact. 2013;26:991-1003 pubmed publisher
    ..Moreover, G? mutants were found to be hypersusceptible to spray inoculation with the bacterial pathogen Pseudomonas syringae. ..
  21. Jiang K, Frick Cheng A, Trusov Y, Delgado Cerezo M, Rosenthal D, Lorek J, et al. Dissecting Arabidopsis G? signal transduction on the protein surface. Plant Physiol. 2012;159:975-83 pubmed publisher
    ..The Arabidopsis (Arabidopsis thaliana) G?-subunit of this complex (AGB1) interacts with and modulates the activity of target cytoplasmic proteins...
  22. Kansup J, Tsugama D, Liu S, Takano T. Arabidopsis G-protein ? subunit AGB1 interacts with NPH3 and is involved in phototropism. Biochem Biophys Res Commun. 2014;445:54-7 pubmed publisher
    ..Here we show that Arabidopsis G? (AGB1) interacts with NPH3, a regulator of phototropism...
  23. Cheng Z, Li J, Niu Y, Zhang X, Woody O, Xiong Y, et al. Pathogen-secreted proteases activate a novel plant immune pathway. Nature. 2015;521:213-6 pubmed publisher
    ..The ability of P. aeruginosa to infect both plants and animals makes it an excellent model to identify novel immunoregulatory strategies that account for its niche adaptation to diverse host tissues and immune systems. ..
  24. Diener A. Visualizing and quantifying Fusarium oxysporum in the plant host. Mol Plant Microbe Interact. 2012;25:1531-41 pubmed publisher
    ..infection below ground, elevated levels of arabinofuranosidase activity were measured in the roots of susceptible agb1 and rfo1 while a reduced level was detected in the resistant eir1. In contrast, disease severity and F...
  25. Llorente F, Alonso Blanco C, Sánchez Rodríguez C, Jorda L, Molina A. ERECTA receptor-like kinase and heterotrimeric G protein from Arabidopsis are required for resistance to the necrotrophic fungus Plectosphaerella cucumerina. Plant J. 2005;43:165-80 pubmed
    ..cucumerina was supported by the fact that three mutants defective in this pathway, elk2, elk5 and elk4 (agb1-1), which encodes the beta-subunit of Arabidopsis heterotrimeric G protein, were also impaired in their resistance ..
  26. Olejnik K, Bucholc M, Anielska Mazur A, Lipko A, Kujawa M, Modzelan M, et al. Arabidopsis thaliana Nudix hydrolase AtNUDT7 forms complexes with the regulatory RACK1A protein and Ggamma subunits of the signal transducing heterotrimeric G protein. Acta Biochim Pol. 2011;58:609-16 pubmed
    ..Moreover, we provide the initial evidence that both signal transducing gamma subunits bind the regulatory RACK1A protein. ..
  27. Yu T, Shi D, Jia P, Tang J, Li H, Liu J, et al. The Arabidopsis Receptor Kinase ZAR1 Is Required for Zygote Asymmetric Division and Its Daughter Cell Fate. PLoS Genet. 2016;12:e1005933 pubmed publisher
    ..After fertilization, ZAR1 is accumulated in zygote and endosperm. The disruption of ZAR1 and AGB1 results in short basal cell and an apical cell with basal cell fate...
  28. Joo J, Wang S, Chen J, Jones A, Fedoroff N. Different signaling and cell death roles of heterotrimeric G protein alpha and beta subunits in the Arabidopsis oxidative stress response to ozone. Plant Cell. 2005;17:957-70 pubmed
  29. Ishida T, Tabata R, Yamada M, Aida M, Mitsumasu K, Fujiwara M, et al. Heterotrimeric G proteins control stem cell proliferation through CLAVATA signaling in Arabidopsis. EMBO Rep. 2014;15:1202-9 pubmed publisher
    ..Our genetic and cell biological analyses suggest that the G protein beta-subunit1 AGB1 and RPK2, one of the major CLV3 peptide hormone receptors, work synergistically in stem cell homeostasis through ..
  30. Xu D, Chen M, Ma Y, Xu Z, Li L, Chen Y, et al. A G-protein β subunit, AGB1, negatively regulates the ABA response and drought tolerance by down-regulating AtMPK6-related pathway in Arabidopsis. PLoS ONE. 2015;10:e0116385 pubmed publisher
    ..In this study, an AGB1 mutant (agb1-2) was found to show enhanced drought tolerance, indicating that AGB1 might negatively regulate ..
  31. Gookin T, Assmann S. Significant reduction of BiFC non-specific assembly facilitates in planta assessment of heterotrimeric G-protein interactors. Plant J. 2014;80:553-67 pubmed publisher
    ..with a calmodulin-like (CML) protein, and by showing that heterotrimeric G-protein subunits Gα (GPA1) and Gβ (AGB1) interact in plant cells...
  32. Yu Y, Chakravorty D, Assmann S. The G Protein β-Subunit, AGB1, Interacts with FERONIA in RALF1-Regulated Stomatal Movement. Plant Physiol. 2018;176:2426-2440 pubmed publisher
    ..thaliana), there are one canonical Gα (GPA1), three extra-large Gα (XLG1, XLG2, and XLG3), one Gβ (AGB1), and three Gγ (AGG1, AGG2, and AGG3) subunits...
  33. Guo J, Wang S, Wang J, Huang W, Liang J, Chen J. Dissection of the relationship between RACK1 and heterotrimeric G-proteins in Arabidopsis. Plant Cell Physiol. 2009;50:1681-94 pubmed publisher
    ..and developmental phenotypes with loss-of-function alleles of GPA1 (encoding the sole Galpha in Arabidopsis) or AGB1 (encoding the sole Gbeta in Arabidopsis)...
  34. Tanaka K, Swanson S, Gilroy S, Stacey G. Extracellular nucleotides elicit cytosolic free calcium oscillations in Arabidopsis. Plant Physiol. 2010;154:705-19 pubmed publisher
    ..The results reveal new molecular details concerning extracellular nucleotide signaling in plants and the importance of fine control of extracellular nucleotide levels to mediate specific plant cell responses. ..
  35. Chen Y, Brandizzi F. AtIRE1A/AtIRE1B and AGB1 independently control two essential unfolded protein response pathways in Arabidopsis. Plant J. 2012;69:266-77 pubmed publisher
    ..We have also established that Arabidopsis AGB1, a subunit of the ubiquitous heterotrimeric GTP-binding protein family, and AtIRE1A/AtIRE1B independently control ..
  36. Torres M, Morales J, Sánchez Rodríguez C, Molina A, Dangl J. Functional interplay between Arabidopsis NADPH oxidases and heterotrimeric G protein. Mol Plant Microbe Interact. 2013;26:686-94 pubmed publisher
    ..The G? subunit (AGB1) of the heterotrimeric G protein is required for full disease resistance to different Pseudomonas syringae strains...
  37. Li S, Liu Y, Zheng L, Chen L, Li N, Corke F, et al. The plant-specific G protein ? subunit AGG3 influences organ size and shape in Arabidopsis thaliana. New Phytol. 2012;194:690-703 pubmed publisher
    ..analyses revealed that AGG3 action requires a functional G protein ? subunit (GPA1) and G protein ? subunit (AGB1)...
  38. Maruta N, Trusov Y, Brenya E, Parekh U, Botella J. Membrane-localized extra-large G proteins and Gbg of the heterotrimeric G proteins form functional complexes engaged in plant immunity in Arabidopsis. Plant Physiol. 2015;167:1004-16 pubmed
    ..Accordingly, XLGs expand the repertoire of heterotrimeric G proteins in plants and reveal a higher level of diversity in heterotrimeric G protein signaling. ..
  39. Adjobo Hermans M, Goedhart J, Gadella T. Plant G protein heterotrimers require dual lipidation motifs of Galpha and Ggamma and do not dissociate upon activation. J Cell Sci. 2006;119:5087-97 pubmed
    ..Importantly, rendering GPalpha1 constitutively active did not cause a FRET decrease in the heterotrimer, suggesting no dissociation upon GPalpha1 activation. ..
  40. Delgado Cerezo M, Sánchez Rodríguez C, Escudero V, Miedes E, Fernández P, Jorda L, et al. Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi. Mol Plant. 2012;5:98-114 pubmed publisher
    The Arabidopsis heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi. The agb1 mutant impaired in the G? subunit displays enhanced susceptibility to these pathogens...
  41. Chakravorty D, Botella J. Over-expression of a truncated Arabidopsis thaliana heterotrimeric G protein gamma subunit results in a phenotype similar to alpha and beta subunit knockouts. Gene. 2007;393:163-70 pubmed
  42. Roy Choudhury S, Pandey S. Recently duplicated plant heterotrimeric G? proteins with subtle biochemical differences influence specific outcomes of signal-response coupling. J Biol Chem. 2017;292:16188-16198 pubmed publisher
  43. Cote G, Luo X, Murphy M, Egelhoff T. Mapping of the novel protein kinase catalytic domain of Dictyostelium myosin II heavy chain kinase A. J Biol Chem. 1997;272:6846-9 pubmed
    ..We suggest that MHCK A represents the prototype for a novel, widely occurring protein kinase family. ..