Gene Symbol: AFB2
Description: auxin signaling F-box 2
Alias: auxin signaling F-box 2, auxin signaling F-box 2
Species: thale cress
Products:     AFB2

Top Publications

  1. Dharmasiri N, Dharmasiri S, Estelle M. The F-box protein TIR1 is an auxin receptor. Nature. 2005;435:441-5 pubmed
    ..Finally, TIR1 synthesized in insect cells binds Aux/IAA proteins in an auxin-dependent manner. Together, these results indicate that TIR1 is an auxin receptor that mediates Aux/IAA degradation and auxin-regulated transcription. ..
  2. Terrile M, París R, Calderon Villalobos L, Iglesias M, Lamattina L, Estelle M, et al. Nitric oxide influences auxin signaling through S-nitrosylation of the Arabidopsis TRANSPORT INHIBITOR RESPONSE 1 auxin receptor. Plant J. 2012;70:492-500 pubmed publisher
    ..Our findings underline the importance of NO in phytohormone signaling pathways. ..
  3. Dharmasiri N, Dharmasiri S, Weijers D, Lechner E, Yamada M, Hobbie L, et al. Plant development is regulated by a family of auxin receptor F box proteins. Dev Cell. 2005;9:109-19 pubmed
    ..Correspondingly, all TIR1/AFB proteins interact with BDL, and BDL is stabilized in triple mutant plants. Our results indicate that TIR1 and the AFB proteins collectively mediate auxin responses throughout plant development. ..
  4. Navarro L, Dunoyer P, Jay F, Arnold B, Dharmasiri N, Estelle M, et al. A plant miRNA contributes to antibacterial resistance by repressing auxin signaling. Science. 2006;312:436-9 pubmed
    ..induces a plant microRNA (miRNA) that negatively regulates messenger RNAs for the F-box auxin receptors TIR1, AFB2, and AFB3. Repression of auxin signaling restricts P...
  5. Parry G, Calderon Villalobos L, Prigge M, Peret B, Dharmasiri S, Itoh H, et al. Complex regulation of the TIR1/AFB family of auxin receptors. Proc Natl Acad Sci U S A. 2009;106:22540-5 pubmed publisher
    ..Our results indicate that the TIR1/AFB proteins have distinct biochemical activities and that TIR1 and AFB2 are the dominant auxin receptors in the seedling root...
  6. Si Ammour A, Windels D, Arn Bouldoires E, Kutter C, Ailhas J, Meins F, et al. miR393 and secondary siRNAs regulate expression of the TIR1/AFB2 auxin receptor clade and auxin-related development of Arabidopsis leaves. Plant Physiol. 2011;157:683-91 pubmed publisher
    ..Thus, miR393 and possibly siTAARs regulate auxin perception and certain auxin-related aspects of leaf development. ..
  7. Calderon Villalobos L, Lee S, de Oliveira C, Ivetac A, Brandt W, Armitage L, et al. A combinatorial TIR1/AFB-Aux/IAA co-receptor system for differential sensing of auxin. Nat Chem Biol. 2012;8:477-85 pubmed publisher
    ..This co-receptor system broadens the effective concentration range of the hormone and may contribute to the complexity of auxin response. ..
  8. Cecchetti V, Altamura M, Falasca G, Costantino P, Cardarelli M. Auxin regulates Arabidopsis anther dehiscence, pollen maturation, and filament elongation. Plant Cell. 2008;20:1760-74 pubmed publisher
    ..In agreement with the timing of auxin effects, the TIR1, AFB1, AFB2, and AFB3 auxin receptor-encoding genes are transcribed in anthers only during late stages of development starting ..
  9. Iglesias M, Terrile M, Casalongué C. Auxin and salicylic acid signalings counteract the regulation of adaptive responses to stress. Plant Signal Behav. 2011;6:452-4 pubmed
    ..In particular, the double mutant for TIR1 and AFB2 receptors, tir1 afb2 displayed increased tolerance against salinity measured as germination rate, root elongation ..

More Information


  1. Iglesias M, Terrile M, Windels D, Lombardo M, Bartoli C, Vazquez F, et al. MiR393 regulation of auxin signaling and redox-related components during acclimation to salinity in Arabidopsis. PLoS ONE. 2014;9:e107678 pubmed publisher
    ..We have previously reported that the Arabidopsis double mutant tir1 afb2 is more tolerant to salt stress than wild-type plants and we hypothesized that down-regulation of auxin signaling ..
  2. Wójcik A, Gaj M. miR393 contributes to the embryogenic transition induced in vitro in Arabidopsis via the modification of the tissue sensitivity to auxin treatment. Planta. 2016;244:231-43 pubmed publisher
    miR393 was found to control embryogenic transition in somatic cells in Arabidopsis via control of the TIR1 and AFB2 auxin receptors genes of the F-box family...
  3. Takato S, Kakei Y, Mitsui M, Ishida Y, Suzuki M, Yamazaki C, et al. Auxin signaling through SCFTIR1/AFBs mediates feedback regulation of IAA biosynthesis. Biosci Biotechnol Biochem. 2017;81:1320-1326 pubmed publisher
    ..These observations suggest that the endogenous IAA levels are regulated by auxin biosynthesis in a feedback manner, and the Aux/IAA and SCFTIR1/AFB-mediated auxin-signaling pathway regulates the expression of YUC genes. ..