Gene Symbol: SSO_RS05190
Description: DNA primase
Species: Sulfolobus solfataricus P2
Lao Sirieix S, Bell S. The heterodimeric primase of the hyperthermophilic archaeon Sulfolobus solfataricus possesses DNA and RNA primase, polymerase and 3'-terminal nucleotidyl transferase activities. J Mol Biol. 2004;344:1251-63 pubmed
..In addition to the primase and polymerase activities, we reveal that the primase possesses a template-independent 3'-terminal nucleotidyl transferase activity. ..
Lao Sirieix S, Nookala R, Roversi P, Bell S, Pellegrini L. Structure of the heterodimeric core primase. Nat Struct Mol Biol. 2005;12:1137-44 pubmed publisher
..Modeling of a DNA-RNA helix together with structure-based site-directed mutagenesis provides insight into the mechanism of template DNA binding and RNA primer synthesis...
Wu K, Lai X, Guo X, Hu J, Xiang X, Huang L. Interplay between primase and replication factor C in the hyperthermophilic archaeon Sulfolobus solfataricus. Mol Microbiol. 2007;63:826-37 pubmed
..These findings suggest that the primase-RFC interaction modulates the activities of both enzymes and therefore may be involved in the regulation of primer synthesis and the transfer of primers to DNA polymerase in Archaea. ..
Hu J, Guo L, Wu K, Liu B, Lang S, Huang L. Template-dependent polymerization across discontinuous templates by the heterodimeric primase from the hyperthermophilic archaeon Sulfolobus solfataricus. Nucleic Acids Res. 2012;40:3470-83 pubmed publisher
..Our results suggest that SsoPriSL is capable of bridging noncomplementary DNA ends and, therefore, may serve a role in double-strand DNA break repair in Archaea...
Holzer S, Yan J, Kilkenny M, Bell S, Pellegrini L. Primer synthesis by a eukaryotic-like archaeal primase is independent of its Fe-S cluster. Nat Commun. 2017;8:1718 pubmed publisher
..Our evidence shows that PriX has replaced PriL as the subunit that endows primase with the unique ability to initiate nucleic acid synthesis. Importantly, our findings reveal that the Fe-S cluster is not required for primer synthesis. ..