Gene Symbol: SAP033A_029
Description: Arsenate reductase
Species: Staphylococcus aureus
Ji G, Silver S. Reduction of arsenate to arsenite by the ArsC protein of the arsenic resistance operon of Staphylococcus aureus plasmid pI258. Proc Natl Acad Sci U S A. 1992;89:9474-8 pubmed
..Purified ArsC protein coupled in vitro with thioredoxin plus dithiothreitol (but not 2-mercaptoethanol or reduced glutathione) to reduce arsenate to arsenite...
Ji G, Silver S. Regulation and expression of the arsenic resistance operon from Staphylococcus aureus plasmid pI258. J Bacteriol. 1992;174:3684-94 pubmed
..Partial deletion of 56 bp from the 3' end of the arsC gene resulted in loss of resistance to arsenate; the determinant retained arsenite and antimonite resistances...
Ji G, Garber E, Armes L, Chen C, Fuchs J, Silver S. Arsenate reductase of Staphylococcus aureus plasmid pI258. Biochemistry. 1994;33:7294-9 pubmed
..0 Da, suggesting that 70% of the purified protein lacked the N-terminal three amino acids; HPLC coupled to electrospray mass spectroscopy of protease digest products confirmed this conclusion and verified the entire amino acid sequence...
Messens J, Hayburn G, Desmyter A, Laus G, Wyns L. The essential catalytic redox couple in arsenate reductase from Staphylococcus aureus. Biochemistry. 1999;38:16857-65 pubmed
..From the peptide-mass profiles, cysteines 82 and 89 were identified as the redox couple of ArsC necessary to reduce arsenate to arsenite...
Zegers I, Martins J, Willem R, Wyns L, Messens J. Arsenate reductase from S. aureus plasmid pI258 is a phosphatase drafted for redox duty. Nat Struct Biol. 2001;8:843-7 pubmed publisher
..The reaction is associated with major structural changes in the ArsC...
Messens J, Martins J, Brosens E, Van Belle K, Jacobs D, Willem R, et al
. Kinetics and active site dynamics of Staphylococcus aureus arsenate reductase. J Biol Inorg Chem. 2002;7:146-56 pubmed
..2 x 10(4 )M-1s-1), while its product, arsenite, was identified as a mixed inhibitor ( K*iu=534 microM, K*ic=377 microM). ..
Messens J, Martins J, Van Belle K, Brosens E, Desmyter A, De Gieter M, et al
. All intermediates of the arsenate reductase mechanism, including an intramolecular dynamic disulfide cascade. Proc Natl Acad Sci U S A. 2002;99:8506-11 pubmed publisher
..Within this cascade, the formation of a disulfide bond triggers a reversible "conformational switch" that transfers the oxidative equivalents to the surface of the protein, while releasing the reduced substrate...