LOX1.1

Summary

Gene Symbol: LOX1.1
Description: lipoxygenase
Alias: Lx1, seed linoleate 13S-lipoxygenase-1, lipoxygenase 1, lipoxygenase L-1, mutant lipoxygenase 1, seed linoleate 9S-lipoxygenase-1
Species: soybean
Products:     LOX1.1

Top Publications

  1. Boyington J, Gaffney B, Amzel L. The three-dimensional structure of an arachidonic acid 15-lipoxygenase. Science. 1993;260:1482-6 pubmed
    ..The iron, with two adjacent and unoccupied positions, is poised to interact with the 1,4-diene system of the substrate and with molecular oxygen during catalysis. ..
  2. Minor W, Steczko J, Stec B, Otwinowski Z, Bolin J, Walter R, et al. Crystal structure of soybean lipoxygenase L-1 at 1.4 A resolution. Biochemistry. 1996;35:10687-701 pubmed
    ..Structural restrictions pertinent to other proposed reaction intermediates, such as planar pentadienyl and nonplanar allyl radicals, are also discussed. ..
  3. Steczko J, Axelrod B. Identification of the iron-binding histidine residues in soybean lipoxygenase L-1. Biochem Biophys Res Commun. 1992;186:686-9 pubmed
    ..We have now found that these inactive proteins contain no Fe, although they have the same electrophoretic mobility as wild-type L-1 under both denaturing and non-denaturing conditions and react with anti-L-1 antibodies. ..
  4. Steczko J, Donoho G, Clemens J, Dixon J, Axelrod B. Conserved histidine residues in soybean lipoxygenase: functional consequences of their replacement. Biochemistry. 1992;31:4053-7 pubmed
    ..The inactive mutants, H499Q, H504Q, H504S, and H690Q, become insoluble when heated for 3 min at 65 degrees C, as does H522Q. The other mutants and the wild-type are stable under these conditions.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  5. Shibata D, Steczko J, Dixon J, Hermodson M, Yazdanparast R, Axelrod B. Primary structure of soybean lipoxygenase-1. J Biol Chem. 1987;262:10080-5 pubmed
    ..The enzyme has a molecular weight of 94,038 based on its content of 838 amino acids. ..
  6. Minor W, Steczko J, Bolin J, Otwinowski Z, Axelrod B. Crystallographic determination of the active site iron and its ligands in soybean lipoxygenase L-1. Biochemistry. 1993;32:6320-3 pubmed
    ..A sixth protein ligand is not present, and the sixth coordination site opens into a wide cleft. The structure of the soybean lipoxygenase was solved by multiple anomalous isomorphous replacements. ..
  7. Tomchick D, Phan P, Cymborowski M, Minor W, Holman T. Structural and functional characterization of second-coordination sphere mutants of soybean lipoxygenase-1. Biochemistry. 2001;40:7509-17 pubmed
    ..The unusual and dramatic inverse solvent isotope effect (SIE) observed for the Q697E mutant indicated that an Fe(III)-OH(-) is the active site base. A new transition state model for hydrogen atom abstraction is proposed...
  8. Chechetkin I, Osipova E, Tarasova N, Mukhitova F, Hamberg M, Gogolev Y, et al. Specificity of oxidation of linoleic acid homologs by plant lipoxygenases. Biochemistry (Mosc). 2009;74:855-61 pubmed
    ..Our findings also demonstrate that the distance between carboxyl group and double bonds substantially determines the positioning of substrates within the active site. ..
  9. Bradshaw M, Gaffney B. Fluctuations of an exposed ?-helix involved in lipoxygenase substrate recognition. Biochemistry. 2014;53:5102-10 pubmed publisher
    ..We interpret these data to mean that a ?-helix in the second helix of plant lipoxygenases is highly dynamic and is the site where lipid chains penetrate to inner helices that outline the substrate pocket. ..

More Information

Publications11

  1. Phatak P, Venderley J, Debrota J, Li J, Iyengar S. Active Site Dynamical Effects in the Hydrogen Transfer Rate-limiting Step in the Catalysis of Linoleic Acid by Soybean Lipoxygenase-1 (SLO-1): Primary and Secondary Isotope Contributions. J Phys Chem B. 2015;119:9532-46 pubmed publisher
    ..This will modify the bulkiness of hydrogen the bonding residue, allowing greater flexibility in the secondary hydrogen bond formation highlighted above and adversely affecting the reaction rate. ..
  2. Collazo L, Klinman J. Control of the Position of Oxygen Delivery in Soybean Lipoxygenase-1 by Amino Acid Side Chains within a Gas Migration Channel. J Biol Chem. 2016;291:9052-9 pubmed publisher
    ..investigates the role of a defined pathway for O2 in regulating the peroxidation of linoleic acid by soybean lipoxygenase 1. Computational and mutagenesis studies provide strong support for a dominant delivery channel that shuttles ..