Gene Symbol: NOS3
Description: nitric oxide synthase 3
Alias: 23B, EC-NOS, NOSIII, cNOS, eNOS, nitric oxide synthase, endothelial, LOW QUALITY PROTEIN: nitric oxide synthase, endothelial, NOS type III, constitutive NOS, endothelial NOS, endothelial cell nitric oxide synthase, endothelial nitric oxide synthase
Species: sheep

Top Publications

  1. Li Y, Zheng J, Bird I, Magness R. Mechanisms of shear stress-induced endothelial nitric-oxide synthase phosphorylation and expression in ovine fetoplacental artery endothelial cells. Biol Reprod. 2004;70:785-96 pubmed publisher
    Placental blood flow, nitric-oxide (NO) levels, and endothelial NO synthase (eNOS) expression increase during human and ovine pregnancy...
  2. Boeldt D, Grummer M, Magness R, Bird I. Altered VEGF-stimulated Ca2+ signaling in part underlies pregnancy-adapted eNOS activity in UAEC. J Endocrinol. 2014;223:1-11 pubmed publisher
    ..Previously, vascular endothelial growth factor (VEGF) has been shown to stimulate endothelial nitric oxide synthase (eNOS) in uterine artery endothelial cells (UAECs) derived from pregnant ewes to a greater extent ..
  3. John T, Ibe B, Usha Raj J. Oxygen alters caveolin-1 and nitric oxide synthase-3 functions in ovine fetal and neonatal lung microvascular endothelial cells. Am J Physiol Lung Cell Mol Physiol. 2006;291:L1079-93 pubmed
  4. Monau T, Vargas V, King N, Yellon S, Myers D, Ducsay C. Long-term hypoxia increases endothelial nitric oxide synthase expression in the ovine fetal adrenal. Reprod Sci. 2009;16:865-74 pubmed publisher
    ..and NOS distribution was examined by immunohistochemistry and double-staining immunofluorescence for endothelial NOS (eNOS) and 17alpha-hydroxylase (CYP17)...
  5. Pastore M, Talwar S, Conley M, Magness R. Identification of Differential ER-Alpha Versus ER-Beta Mediated Activation of eNOS in Ovine Uterine Artery Endothelial Cells. Biol Reprod. 2016;94:139 pubmed publisher
    ..Estradiol induces endothelial NO synthase (eNOS) activation to increase NO production; however, it is unknown if eNOS regulation is dependent on both ERs...
  6. Kameny R, He Y, Morris C, Sun C, Johengen M, Gong W, et al. Right ventricular nitric oxide signaling in an ovine model of congenital heart disease: a preserved fetal phenotype. Am J Physiol Heart Circ Physiol. 2015;309:H157-65 pubmed publisher
    ..These data demonstrate preserved fetal NOS phenotype and NO signaling in shunt RV, which may partially explain the mechanism underlying the adaptive response to increased afterload seen in the RV of shunt lambs. ..
  7. Wang X, Frank J, Xu J, Dunlap K, Satterfield M, Burghardt R, et al. Functional role of arginine during the peri-implantation period of pregnancy. II. Consequences of loss of function of nitric oxide synthase NOS3 mRNA in ovine conceptus trophectoderm. Biol Reprod. 2014;91:59 pubmed publisher in vivo morpholino antisense oligonucleotide (MAO)-mediated knockdown trial of nitric oxide synthase-3 (NOS3) mRNA, the major isoform of NO synthase, in ovine conceptus trophectoderm (Tr)...
  8. Teng R, Wu T, Bisig C, Eis A, Pritchard K, Konduri G. Nitrotyrosine impairs angiogenesis and uncouples eNOS activity of pulmonary artery endothelial cells isolated from developing sheep lungs. Pediatr Res. 2011;69:112-7 pubmed publisher
    ..Effects of NT on endothelial NO synthase (eNOS) were examined with respect to eNOS-dimer formation and the association of eNOS chaperone, heat-shock-protein-90 (..
  9. Mata Greenwood E, Liao W, Zheng J, Chen D. Differential activation of multiple signalling pathways dictates eNOS upregulation by FGF2 but not VEGF in placental artery endothelial cells. Placenta. 2008;29:708-17 pubmed publisher
    Fibroblast growth factor (FGF2), but not vascular endothelial growth factor (VEGF), upregulates endothelial nitric oxide synthase (eNOS) protein expression, at least partially, via activation of extracellular signal-regulated kinase 2/1 (..

More Information


  1. John T, Ibe B, Raj J. Regulation of endothelial nitric oxide synthase: involvement of protein kinase G 1 beta, serine 116 phosphorylation and lipid structures. Clin Exp Pharmacol Physiol. 2008;35:148-58 pubmed
    1. Endothelial nitric oxide synthase (NOS3) is important for vascular homeostasis...
  2. Grummer M, Sullivan J, Magness R, Bird I. Vascular endothelial growth factor acts through novel, pregnancy-enhanced receptor signalling pathways to stimulate endothelial nitric oxide synthase activity in uterine artery endothelial cells. Biochem J. 2009;417:501-11 pubmed publisher
    ..VEGF receptors) and associated signalling pathways mediating the effects of VEGF(165) on eNOS (endothelial nitric oxide synthase) activation...
  3. Cox R, Jacob S, Oliveras G, Murakami K, Enkhbaatar P, Traber L, et al. Pulmonary expression of nitric oxide synthase isoforms in sheep with smoke inhalation and burn injury. Exp Lung Res. 2009;35:104-18 pubmed publisher
    ..The study indicates that in uninjured sheep lung, endothelial (eNOS) is constitutively expressed in the endothelial cells associated with the airways and parenchyma, and in ..
  4. Sud N, Sharma S, Wiseman D, Harmon C, Kumar S, Venema R, et al. Nitric oxide and superoxide generation from endothelial NOS: modulation by HSP90. Am J Physiol Lung Cell Mol Physiol. 2007;293:L1444-53 pubmed
    ..These data indicated that a certain degree of uncoupling of endothelial NO synthase (eNOS) occurs in PAECs during postnatal development...
  5. Yi F, Boeldt D, Gifford S, Sullivan J, Grummer M, Magness R, et al. Pregnancy enhances sustained Ca2+ bursts and endothelial nitric oxide synthase activation in ovine uterine artery endothelial cells through increased connexin 43 function. Biol Reprod. 2010;82:66-75 pubmed publisher
    ..culture still show a pregnancy-enhanced difference in ATP-stimulated endothelial NO synthase (eNOS; official symbol NOS3) activation, even though NOS3 protein, purinergic receptors, and associated cell signaling proteins are expressed ..
  6. Konduri G, Bakhutashvili I, Eis A, Pritchard K. Oxidant stress from uncoupled nitric oxide synthase impairs vasodilation in fetal lambs with persistent pulmonary hypertension. Am J Physiol Heart Circ Physiol. 2007;292:H1812-20 pubmed publisher
    ..We investigated the hypothesis that increased superoxide (O(2)(*-)) release by an uncoupled endothelial nitric oxide synthase (eNOS) contributes to impaired pulmonary vasodilation in PPHN...
  7. Yi F, Boeldt D, Magness R, Bird I. [Ca2+]i signaling vs. eNOS expression as determinants of NO output in uterine artery endothelium: relative roles in pregnancy adaptation and reversal by VEGF165. Am J Physiol Heart Circ Physiol. 2011;300:H1182-93 pubmed publisher changes in NO in response to ionomycin, suggesting this is solely due to higher levels of endothelial nitric oxide synthase (eNOS) protein in the follicular phase...
  8. Teng R, Rana U, Afolayan A, Zhao B, Miao Q, Konduri G. Nogo-B receptor modulates angiogenesis response of pulmonary artery endothelial cells through eNOS coupling. Am J Respir Cell Mol Biol. 2014;51:169-77 pubmed publisher
    ..PAECs rescued the in vitro angiogenesis defects and increased the phosphorylation of both Akt and endothelial nitric oxide synthase at serine(1179) as well as the levels of both manganese superoxide dismutase and GTP cyclohydrolase-..
  9. Grobe A, Wells S, Benavidez E, Oishi P, Azakie A, Fineman J, et al. Increased oxidative stress in lambs with increased pulmonary blood flow and pulmonary hypertension: role of NADPH oxidase and endothelial NO synthase. Am J Physiol Lung Cell Mol Physiol. 2006;290:L1069-77 pubmed
    ..As DPI can also inhibit endothelial nitric oxide synthase (eNOS) superoxide generation, we repeated this experiment using a more specific NADPH oxidase ..
  10. Sud N, Kumar S, Wedgwood S, Black S. Modulation of PKCdelta signaling alters the shear stress-mediated increases in endothelial nitric oxide synthase transcription: role of STAT3. Am J Physiol Lung Cell Mol Physiol. 2009;296:L519-26 pubmed publisher
    We have previously shown that the regulation of endothelial nitric oxide synthase (eNOS) in endothelial cells isolated from fetal lamb under static conditions is positively regulated by PKCdelta...
  11. Gao H, Wu G, Spencer T, Johnson G, Bazer F. Select nutrients in the ovine uterine lumen. V. Nitric oxide synthase, GTP cyclohydrolase, and ornithine decarboxylase in ovine uteri and peri-implantation conceptuses. Biol Reprod. 2009;81:67-76 pubmed publisher
    ..estrous cycle, pregnancy, progesterone, and interferon tau (IFNT) on expression of NO synthases (NOS1, NOS2, and NOS3), guanosine triphosphate (GTP) cyclohydrolase (GCH1, the key enzyme in de novo synthesis of tetrahydrobiopterin, a ..
  12. Sud N, Black S. Endothelin-1 impairs nitric oxide signaling in endothelial cells through a protein kinase Cdelta-dependent activation of STAT3 and decreased endothelial nitric oxide synthase expression. DNA Cell Biol. 2009;28:543-53 pubmed publisher
    ..pulmonary hypertension of the newborn (PPHN), endothelin-1 (ET-1) expression is increased, while endothelial nitric oxide synthase (eNOS) expression is decreased...
  13. John T. Protein kinase G inhibits basal and stimulated nitric oxide synthase activity in neonatal ovine lung microvascular endothelial cells. Afr J Med Med Sci. 2010;39:89-98 pubmed
    ..Both basal and stimulated NO production is regulated by the downstream activation of PKG by NO-induced 8-Br-cGMP production in endothelial cells. ..
  14. Wood C, Chen G, Keller Wood M. Expression of nitric oxide synthase isoforms is reduced in late-gestation ovine fetal brainstem. Am J Physiol Regul Integr Comp Physiol. 2005;289:R613-R619 pubmed publisher
    ..Neuronal (nNOS), inducible (iNOS), and endothelial (eNOS) mRNA was measured using real-time PCR methodology specific for ovine NOS isoforms...
  15. Cale J, Tsoi S, Toppe M, Grummer M, Ochiai M, Magness R, et al. Molecular cloning of ovine endothelial nitric oxide synthase and expression in COS-7 cells. J Soc Gynecol Investig. 2005;12:156-68 pubmed
    While studies of human and bovine endothelial nitric oxide synthase (eNOS) demonstrate activation by Ca(2+)/calmodulin, recent progress demonstrates that eNOS phosphorylation can alter sensitivity to intracellular free calcium ([Ca(2+)](..
  16. Mershon J, Baker R, Clark K. Estrogen increases iNOS expression in the ovine coronary artery. Am J Physiol Heart Circ Physiol. 2002;283:H1169-80 pubmed
    ..Coronary arteries were analyzed for ovine iNOS and endothelial NOS (eNOS) expression by semiquantitative RT-PCR...