secF

Summary

Gene Symbol: secF
Description: SecYEG protein translocase auxillary subunit
Alias: ECK0403, JW0399
Species:

Top Publications

  1. ncbi Alignment of genes from the 9-minute region (araJ to tsx) of the Escherichia coli K-12 linkage map to the physical map
    J M Lucht
    Department of Biology, University of Konstanz, Germany
    J Bacteriol 174:1709-10. 1992
  2. ncbi The SecDFyajC domain of preprotein translocase controls preprotein movement by regulating SecA membrane cycling
    F Duong
    Dartmouth Medical School, Department of Biochemistry, Hanover, NH 03755, USA
    EMBO J 16:4871-9. 1997
  3. ncbi Targeting and assembly of periplasmic and outer-membrane proteins in Escherichia coli
    P N Danese
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Annu Rev Genet 32:59-94. 1998
  4. ncbi YidC mediates membrane protein insertion in bacteria
    J C Samuelson
    Department of Chemistry, The Ohio State University, Columbus 43210, USA
    Nature 406:637-41. 2000
  5. ncbi Protein traffic in bacteria: multiple routes from the ribosome to and across the membrane
    M Muller
    Institute of Biochemistry and Molecular Biology, University of Freiburg, Germany
    Prog Nucleic Acid Res Mol Biol 66:107-57. 2001
  6. ncbi Genetic footprinting in bacteria
    R S Hare
    Schering Plough Research Institute, Kenilworth, New Jersey 07033, USA
    J Bacteriol 183:1694-706. 2001
  7. ncbi SecDFyajC is not required for the maintenance of the proton motive force
    N Nouwen
    Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    FEBS Lett 508:103-6. 2001
  8. ncbi SecDFyajC forms a heterotetrameric complex with YidC
    Nico Nouwen
    Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren, The Netherlands
    Mol Microbiol 44:1397-405. 2002
  9. ncbi Depletion of SecDF-YajC causes a decrease in the level of SecG: implication for their functional interaction
    Yoshihisa Kato
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    FEBS Lett 550:114-8. 2003
  10. ncbi Biogenesis of inner membrane proteins in Escherichia coli
    Joen Luirink
    Department of Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands
    Annu Rev Microbiol 59:329-55. 2005

Scientific Experts

  • Nico Nouwen
  • Joen Luirink
  • P N Danese
  • Kun Xie
  • Eli O van der Sluis
  • F Duong
  • Yoshihisa Kato
  • M Muller
  • R S Hare
  • J C Samuelson
  • W Wickner
  • Arnold J M Driessen
  • Ross E Dalbey
  • J Beckwith
  • Andreas Kuhn
  • Gisela Nagler
  • Dorothee Kiefer
  • Ken ichi Nishiyama
  • Ken-ichi Nishiyama
  • Hajime Tokuda
  • J A Pogliano
  • J R Greene
  • T J Kenney
  • M C Sulavik
  • M Walbridge
  • S S Walker
  • C Houseweart
  • H G Koch
  • K Beck
  • U Schafer
  • T E Dorman
  • H Yu
  • K Baradaran
  • Z Foldes
  • A Motzer
  • K J Shaw
  • G H Shimer
  • L M Guzman
  • F Jiang
  • M Chen
  • A Kuhn
  • K Sagara
  • I Moller
  • G J Phillips
  • A Economou
  • R E Dalbey
  • M Wiedmann
  • S Matsuyama
  • K J Pogliano
  • M Sugai
  • S Mizushima
  • N Kosic
  • C Gardel
  • D B Oliver
  • H C Wu
  • J M Lucht
  • C K Fan
  • Y Fujita
  • A Jacq
  • J Fandl
  • K L Bieker
  • W Boos
  • E Bremer
  • K Johnson
  • T J Silhavy
  • P C Tai
  • J Hunt
  • S Michaelis
  • S Benson

Detail Information

Publications27

  1. ncbi Alignment of genes from the 9-minute region (araJ to tsx) of the Escherichia coli K-12 linkage map to the physical map
    J M Lucht
    Department of Biology, University of Konstanz, Germany
    J Bacteriol 174:1709-10. 1992
  2. ncbi The SecDFyajC domain of preprotein translocase controls preprotein movement by regulating SecA membrane cycling
    F Duong
    Dartmouth Medical School, Department of Biochemistry, Hanover, NH 03755, USA
    EMBO J 16:4871-9. 1997
    Escherichia coli preprotein translocase comprises a membrane-embedded hexameric complex of SecY, SecE, SecG, SecD, SecF and YajC (SecYEGDFyajC) and the peripheral ATPase SecA...
  3. ncbi Targeting and assembly of periplasmic and outer-membrane proteins in Escherichia coli
    P N Danese
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Annu Rev Genet 32:59-94. 1998
    ..We also discuss the various signal transduction systems that monitor extracytoplasmic protein folding and targeting, and we consider how these signal transduction systems may ultimately control these processes...
  4. ncbi YidC mediates membrane protein insertion in bacteria
    J C Samuelson
    Department of Chemistry, The Ohio State University, Columbus 43210, USA
    Nature 406:637-41. 2000
    ..These results provide evidence for an additional component of the translocation machinery that is specialized for the integration of membrane proteins...
  5. ncbi Protein traffic in bacteria: multiple routes from the ribosome to and across the membrane
    M Muller
    Institute of Biochemistry and Molecular Biology, University of Freiburg, Germany
    Prog Nucleic Acid Res Mol Biol 66:107-57. 2001
    ..itself into the SecYE translocon, a process that is strongly assisted by a couple of membrane proteins, SecG, SecD, SecF, YajC, and the proton gradient across the membrane...
  6. ncbi Genetic footprinting in bacteria
    R S Hare
    Schering Plough Research Institute, Kenilworth, New Jersey 07033, USA
    J Bacteriol 183:1694-706. 2001
    ..In addition, the ability of recombinant proteins to complement mutagenized hosts has been evaluated by genetic footprinting using a bacteriophage lambda transposon delivery system...
  7. ncbi SecDFyajC is not required for the maintenance of the proton motive force
    N Nouwen
    Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    FEBS Lett 508:103-6. 2001
    ..The expression of this enzyme complex is repressed by growth on glucose media, the condition used to deplete SecDFyajC. These results demonstrate that SecDFyajC is not required for proton motive force-driven protein translocation...
  8. ncbi SecDFyajC forms a heterotetrameric complex with YidC
    Nico Nouwen
    Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren, The Netherlands
    Mol Microbiol 44:1397-405. 2002
    ..SecD, SecF, and YajC form another heterotrimeric complex that can associate with the SecYEG complex...
  9. ncbi Depletion of SecDF-YajC causes a decrease in the level of SecG: implication for their functional interaction
    Yoshihisa Kato
    Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    FEBS Lett 550:114-8. 2003
    ..SecDF-YajC inhibited disulfide bond formation between two SecG molecules possessing a single cysteine residue. These results suggest functional interaction between SecDF-YajC and SecG...
  10. ncbi Biogenesis of inner membrane proteins in Escherichia coli
    Joen Luirink
    Department of Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands
    Annu Rev Microbiol 59:329-55. 2005
    ..This review deals mainly with recent structural and biochemical data from various experimental systems that offer new insight into the different stages of biogenesis of E. coli IMPs...
  11. ncbi Inactivation of protein translocation by cold-sensitive mutations in the yajC-secDF operon
    Nico Nouwen
    Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    J Bacteriol 187:6852-5. 2005
    ..Here we report that two of these mutations confer this cold-sensitive phenotype by inactivating the SecDF-YajC complex in protein translocation...
  12. ncbi Stepwise evolution of the Sec machinery in Proteobacteria
    Eli O van der Sluis
    Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren, The Netherlands
    Trends Microbiol 14:105-8. 2006
    ..The Sec machinery of Escherichia coli and other Enterobacteriales represents the end product of this stepwise evolution...
  13. ncbi Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme
    F Duong
    Dartmouth Medical School, Department of Biochemistry, Hanover, NH 03755, USA
    EMBO J 16:2756-68. 1997
    ..The proteins encoded by the secD operon--SecD, SecF and YajC--also form an integral membrane heterotrimeric complex (SecDFyajC)...
  14. ncbi SecA membrane cycling at SecYEG is driven by distinct ATP binding and hydrolysis events and is regulated by SecD and SecF
    A Economou
    Dartmouth Medical School, Department of Biochemistry, Hanover, New Hampshire 03755 3844, USA
    Cell 83:1171-81. 1995
    ..This process is regulated both by nucleotide binding and hydrolysis and by the SecD and SecF proteins...
  15. ncbi Processing of lipid-modified prolipoprotein requires energy and sec gene products in vivo
    N Kosic
    Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814 4799
    J Bacteriol 175:6113-7. 1993
    ..e., secA, secE, secY, secD, and secF, and by metabolic poisons which affect proton motive force (PMF)...
  16. ncbi Export of the outer membrane lipoprotein is defective in secD, secE, and secF mutants of Escherichia coli
    M Sugai
    Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814 4799
    J Bacteriol 174:2511-6. 1992
    The export of major outer membrane lipoprotein has been found to be affected in secD, secE, and secF mutants of Escherichia coli, which are defective in protein export in general...
  17. ncbi Overproduction, purification and characterization of SecD and SecF, integral membrane components of the protein translocation machinery of Escherichia coli
    S Matsuyama
    Institute of Applied Microbiology, University of Tokyo, Yayoi, Japan
    Biochim Biophys Acta 1122:77-84. 1992
    SecD and SecF proteins were overproduced by means of recombinant DNA technology. Immunoblot and amino-acid sequencing analysis revealed that the overproduced proteins are SecD and SecF...
  18. ncbi Protein translocation in vitro: biochemical characterization of genetically defined translocation components
    J Fandl
    Department of Fine Structure, Boston Biomedical Research Institute, Massachusetts 02114
    J Bioenerg Biomembr 22:369-87. 1990
    ..working model whereby the translocator complex is composed of the integral membrane proteins SecY, SecD, SecE, and SecF, forming an aqueous channel in the cytoplasmic membrane, and the tightly associated peripheral membrane protein ..
  19. ncbi The secD locus of E.coli codes for two membrane proteins required for protein export
    C Gardel
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115
    EMBO J 9:3209-16. 1990
    ..The secD mutations fall into two complementation groups, defining genes we have named secD and secF. These two genes comprise an operon, the first case of two genes involved in the export process being co-..
  20. ncbi The secD locus of E. coli codes for two membrane proteins required for protein export
    C Gardel
    EMBO J 9:4205-6. 1990
  21. ncbi The genetics of protein secretion in E. coli
    K L Bieker
    Department of Molecular Biology, Princeton University, NJ 08544
    Trends Genet 6:329-34. 1990
    ..Insights from mutant analysis and the biochemical properties of the purified components allows the secretion pathway to be described in some detail. The picture emerging provides a useful paradigm for similar pathways in other organisms...
  22. ncbi secD, a new gene involved in protein export in Escherichia coli
    C Gardel
    J Bacteriol 169:1286-90. 1987
    ..A plasmid carrying secD+ was identified and used to show that the mutations are recessive. The secD gene may code for a component of the cellular export machinery...
  23. ncbi Genetic and molecular characterization of the Escherichia coli secD operon and its products
    K J Pogliano
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115
    J Bacteriol 176:804-14. 1994
    ..We have characterized this operon, and found that, in addition to secD and secF, it contains the upstream gene yajC, but not the genes queA or tgt, in contrast to previous reports...
  24. ncbi Multicopy suppression of cold-sensitive sec mutations in Escherichia coli
    P N Danese
    Department of Molecular Biology, Princeton University, New Jersey 08544 1014, USA
    J Bacteriol 177:4969-73. 1995
    ..In all cases, the mechanism of suppression is unrelated to the process of protein export. We suggest that the multicopy plasmids also suppress the sec mutations by compromising protein synthesis...
  25. ncbi SecF stabilizes SecD and SecY, components of the protein translocation machinery of the Escherichia coli cytoplasmic membrane
    K Sagara
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
    J Bacteriol 176:4111-6. 1994
    The effect of the overproduction of SecF encoded by the tac-secF gene on a plasmid on the synthesis of other Sec proteins was studied in Escherichia coli...
  26. ncbi SecD and SecF facilitate protein export in Escherichia coli
    J A Pogliano
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115
    EMBO J 13:554-61. 1994
    ..here that the rate of protein translocation in the bacterium Escherichia coli depends on the levels of the SecD and SecF proteins in the cell...
  27. ncbi Different regions of the nonconserved large periplasmic domain of Escherichia coli YidC are involved in the SecF interaction and membrane insertase activity
    Kun Xie
    Department of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA
    Biochemistry 45:13401-8. 2006
    ..includes residues 24-346, is required for the interaction of YidC with the Sec translocase, in particular with the SecF protein. Further studies showed that residues 215-265 of YidC are sufficient for SecF binding...