Gene Symbol: hin
Species: Salmonella typhimurium LT2
Chiu T, Sohn C, Dickerson R, Johnson R. Testing water-mediated DNA recognition by the Hin recombinase. EMBO J. 2002;21:801-14 pubmed
The Hin recombinase specifically recognizes its DNA-binding site by means of both major and minor groove interactions...
Zieg J, Simon M. Analysis of the nucleotide sequence of an invertible controlling element. Proc Natl Acad Sci U S A. 1980;77:4196-200 pubmed
..The gene which specifies H2 flagellin synthesis begins 16 bp outsie the inversion region. Within the inversion region, an open translational frame exists which could encode a low molecualr weight polypeptide (190 amino acids). ..
Heichman K, Johnson R. The Hin invertasome: protein-mediated joining of distant recombination sites at the enhancer. Science. 1990;249:511-7 pubmed
The Hin protein binds to two cis-acting recombination sites and catalyzes a site-specific DNA inversion reaction that regulates the expression of flagellin genes in Salmonella...
Feng J, Johnson R, Dickerson R. Hin recombinase bound to DNA: the origin of specificity in major and minor groove interactions. Science. 1994;263:348-55 pubmed
The structure of the 52-amino acid DNA-binding domain of the prokaryotic Hin recombinase, complexed with a DNA recombination half-site, has been solved by x-ray crystallography at 2.3 angstrom resolution...
Silverman M, Zieg J, Mandel G, Simon M. Analysis of the functional components of the phase variation system. Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:17-26 pubmed
Koskiniemi S, Gibbons H, Sandegren L, Anwar N, Ouellette G, Broomall S, et al
. Pathoadaptive mutations in Salmonella enterica isolated after serial passage in mice. PLoS ONE. 2013;8:e70147 pubmed publisher
..Also, all mouse-adapted lineages had changed the orientation of the hin invertable element, resulting in production of a FliC type of flagellum...
McLean M, Chang Y, Dhar G, Heiss J, Johnson R. Multiple interfaces between a serine recombinase and an enhancer control site-specific DNA inversion. elife. 2013;2:e01211 pubmed publisher
Serine recombinases are often tightly controlled by elaborate, topologically-defined, nucleoprotein complexes. Hin is a member of the DNA invertase subclass of serine recombinases that are regulated by a remote recombinational enhancer ..
Simon M, Zieg J, Silverman M, Mandel G, Doolittle R. Phase variation: evolution of a controlling element. Science. 1980;209:1370-4 pubmed
..The invertible segment is flanked by two sites that are necessary for the inversion and contains a gene (hin) whose product mediates the inversion event...
Feng J, Simon M, Mack D, Dervan P, Johnson R, Dickerson R. Crystallization and preliminary X-ray analysis of the DNA binding domain of the Hin recombinase with its DNA binding site. J Mol Biol. 1993;232:982-6 pubmed
The Hin recombinase catalyzes site-specific inversion of DNA. Chemical and genetic studies on Hin binding to the recombination sites indicates that both major and minor DNA groove interactions are critical...