Gene Symbol: ZDS1
Description: Zds1p
Alias: CES1, CKM1, NRC1, OSS1, Zds1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Ma X, Lu Q, Grunstein M. A search for proteins that interact genetically with histone H3 and H4 amino termini uncovers novel regulators of the Swe1 kinase in Saccharomyces cerevisiae. Genes Dev. 1996;10:1327-40 pubmed
    ..In addition, we have isolated a dosage-dependent suppressor, OSS1, of hsl1 and hsl7. OSS1 is important for the transcriptional repression of SWE1 and CLN2 in G2...
  2. Estruch F, Hodge C, Rodriguez Navarro S, Cole C. Physical and genetic interactions link the yeast protein Zds1p with mRNA nuclear export. J Biol Chem. 2005;280:9691-7 pubmed
    ..Here we reported that Dbp5p and Gfd1p interact with Zds1p, a protein previously identified as a multicopy suppressor in several yeast genetic screens...
  3. Roy N, Runge K. The ZDS1 and ZDS2 proteins require the Sir3p component of yeast silent chromatin to enhance the stability of short linear centromeric plasmids. Chromosoma. 1999;108:146-61 pubmed
    ..Two-hybrid analysis indicated that Zds1p and Zds2p interact with Sir2p, Sir3p, Sir4p or the yeast telomere binding protein Rap1p...
  4. Yasutis K, Vignali M, Ryder M, Tameire F, Dighe S, Fields S, et al. Zds2p regulates Swe1p-dependent polarized cell growth in Saccharomyces cerevisiae via a novel Cdc55p interaction domain. Mol Biol Cell. 2010;21:4373-86 pubmed publisher
    Deletion of the paralogs ZDS1 and ZDS2 in the budding yeast Saccharomyces cerevisiae causes a mis-regulation of polarized cell growth...
  5. Roy N, Runge K. Two paralogs involved in transcriptional silencing that antagonistically control yeast life span. Curr Biol. 2000;10:111-4 pubmed
    ..As Zds2p, but not Zds1p, had strong two-hybrid interactions with Orc1p and the four Sir proteins, Zds1p might indirectly control Sir3p ..
  6. Griffioen G, Branduardi P, Ballarini A, Anghileri P, Norbeck J, Baroni M, et al. Nucleocytoplasmic distribution of budding yeast protein kinase A regulatory subunit Bcy1 requires Zds1 and is regulated by Yak1-dependent phosphorylation of its targeting domain. Mol Cell Biol. 2001;21:511-23 pubmed
    ..two-hybrid screen aimed to isolate genes encoding proteins that interact with the Bcy1 N-terminal domain identified Zds1. In ethanol-grown zds1 cells, cytoplasmic localization of Bcy1 was largely absent, while overexpression of ZDS1 led ..
  7. Bi E, Pringle J. ZDS1 and ZDS2, genes whose products may regulate Cdc42p in Saccharomyces cerevisiae. Mol Cell Biol. 1996;16:5264-75 pubmed
    ..project identified a homolog, ZDS2, whose predicted product of 942 amino acids is 38% identical in sequence to Zds1p. Zds1p and Zds2p have no detectable homology to known Rho-GAPs or to other known proteins...
  8. Wicky S, Tjandra H, Schieltz D, Yates J, Kellogg D. The Zds proteins control entry into mitosis and target protein phosphatase 2A to the Cdc25 phosphatase. Mol Biol Cell. 2011;22:20-32 pubmed publisher
    ..We show here that related proteins called Zds1 and Zds2 form a tight stoichiometric complex with PP2A(Cdc55) and target its activity to Cdc25 but not to Wee1...
  9. Yokoyama H, Mizunuma M, Okamoto M, Yamamoto J, Hirata D, Miyakawa T. Involvement of calcineurin-dependent degradation of Yap1p in Ca2+-induced G2 cell-cycle regulation in Saccharomyces cerevisiae. EMBO Rep. 2006;7:519-24 pubmed
    ..We isolated the YAP1 gene as a multicopy suppressor of calcium sensitivity owing to the loss of ZDS1, a negative regulator of SWE1 and CLN2 gene expression...

More Information


  1. Calabria I, Baro B, Rodriguez Rodriguez J, Russiñol N, Queralt E. Zds1 regulates PP2A(Cdc55) activity and Cdc14 activation during mitotic exit through its Zds_C motif. J Cell Sci. 2012;125:2875-84 pubmed publisher
    ..Separase, together with Zds1p, promotes the downregulation of the protein phosphatase 2A in conjunction with its Cdc55p regulatory subunit (PP2A(..
  2. Zanelli C, Valentini S. Pkc1 acts through Zds1 and Gic1 to suppress growth and cell polarity defects of a yeast eIF5A mutant. Genetics. 2005;171:1571-81 pubmed
    ..Furthermore, two new suppressor genes, ZDS1 and GIC1, were identified. We demonstrated that ZDS1 and ZDS2 are necessary for PKC1, but not for GIC1 suppression...
  3. Queralt E, Uhlmann F. Separase cooperates with Zds1 and Zds2 to activate Cdc14 phosphatase in early anaphase. J Cell Biol. 2008;182:873-83 pubmed publisher
    ..How separase causes PP2A(Cdc55) down-regulation is not known. Here, we show that two Cdc55-interacting proteins, Zds1 and Zds2, contribute to timely Cdc14 activation during mitotic exit...
  4. Mizunuma M, Hirata D, Miyahara K, Tsuchiya E, Miyakawa T. Role of calcineurin and Mpk1 in regulating the onset of mitosis in budding yeast. Nature. 1998;392:303-6 pubmed
    ..Zds1 (also known as Oss1 and Hst1) is important in repressing the transcription of SWE1 in G2 phase...
  5. Bandhakavi S, McCann R, Hanna D, Glover C. Genetic interactions among ZDS1,2, CDC37, and protein kinase CK2 in Saccharomyces cerevisiae. FEBS Lett. 2003;554:295-300 pubmed
    We report here the identification of the homologous gene pair ZDS1,2 as multicopy suppressors of a temperature-sensitive allele (cka2-13(ts)) of the CKA2 gene encoding the alpha' catalytic subunit of protein kinase CK2...
  6. Mizunuma M, Hirata D, Miyaoka R, Miyakawa T. GSK-3 kinase Mck1 and calcineurin coordinately mediate Hsl1 down-regulation by Ca2+ in budding yeast. EMBO J. 2001;20:1074-85 pubmed
    ..The Ca2+-activated pathway may play a role in the rapid inactivation of Hsl1 at the cell cycle stage(s) when APC activity is low. ..
  7. Heo S, Tatebayashi K, Ikeda H. The budding yeast cohesin gene SCC1/MCD1/RHC21 genetically interacts with PKA, CDK and APC. Curr Genet. 1999;36:329-38 pubmed
    ..Thirdly, ZDS1, a multicopy suppressor of cdc28-1N, and its homologue ZDS2 were isolated as multicopy suppressors of rhc21-sk16...
  8. Sekiya Kawasaki M, Abe M, Saka A, Watanabe D, Kono K, Minemura Asakawa M, et al. Dissection of upstream regulatory components of the Rho1p effector, 1,3-beta-glucan synthase, in Saccharomyces cerevisiae. Genetics. 2002;162:663-76 pubmed
    ..We demonstrate that all of the multicopy suppressors obtained (WSC1, WSC3, MTL1, ROM2, LRE1, ZDS1, and MSB1) and the constitutively active RHO1 mutations tested restore 1,3-beta-glucan synthesis in the GS mutant...
  9. Schwer B, Linder P, Shuman S. Effects of deletion mutations in the yeast Ces1 protein on cell growth and morphology and on high copy suppression of mutations in mRNA capping enzyme and translation initiation factor 4A. Nucleic Acids Res. 1998;26:803-9 pubmed
    The homologous Saccharomyces cerevisiae genes CES1 and CES4 act as high copy suppressors of temperature-sensitive mutations of Ceg1p, the yeast mRNA capping enzyme. Neither CES1 nor CES4 is essential for cell growth...
  10. Rossio V, Yoshida S. Spatial regulation of Cdc55-PP2A by Zds1/Zds2 controls mitotic entry and mitotic exit in budding yeast. J Cell Biol. 2011;193:445-54 pubmed publisher
    ..temporal regulation of PP2A is poorly understood, although recent studies demonstrated that the conserved proteins Zds1 and Zds2 stoichiometrically bind to Cdc55-PP2A and regulate it in a complex manner...
  11. Yu Y, Jiang Y, Wellinger R, Carlson K, Roberts J, Stillman D. Mutations in the homologous ZDS1 and ZDS2 genes affect cell cycle progression. Mol Cell Biol. 1996;16:5254-63 pubmed
    The Saccharomyces cerevisiae ZDS1 and ZDS2 genes were identified as multicopy suppressors in distinct genetic screens but were found to encode highly similar proteins...
  12. Petty E, Lafon A, Tomlinson S, Mendelsohn B, Pillus L. Promotion of Cell Viability and Histone Gene Expression by the Acetyltransferase Gcn5 and the Protein Phosphatase PP2A in Saccharomyces cerevisiae. Genetics. 2016;203:1693-707 pubmed publisher
    ..These data highlight the potential significance of dynamic phosphorylation and dephosphorylation of these deeply conserved histone residues for cell viability. ..
  13. Imazu H, Sakurai H. Saccharomyces cerevisiae heat shock transcription factor regulates cell wall remodeling in response to heat shock. Eukaryot Cell. 2005;4:1050-6 pubmed
    ..Several of the other suppressors were found to encode proteins functioning in cell wall organization. These results suggest that Hsf1 in concert with Pkc1 regulates cell wall remodeling in response to heat shock. ..
  14. Hosotani T, Koyama H, Uchino M, Miyakawa T, Tsuchiya E. PKC1, a protein kinase C homologue of Saccharomyces cerevisiae, participates in microtubule function through the yeast EB1 homologue, BIM1. Genes Cells. 2001;6:775-88 pubmed
    ..These results suggest that Pkc1p plays a role which is relevant to microtubule functions and that this role is mediated by a hitherto unknown PKC signalling pathway and by Bim1p ..
  15. Walowsky C, Fitzhugh D, Castaño I, Ju J, Levin N, Christman M. The topoisomerase-related function gene TRF4 affects cellular sensitivity to the antitumor agent camptothecin. J Biol Chem. 1999;274:7302-8 pubmed
    ..another member of the TRF4 gene family, TRF5, and two genes that may influence higher order chromosome structure, ZDS1 and ZDS2. We have isolated and sequenced two human TRF4 family members, hTRF4-1 and hTRF4-2...
  16. Tsubakiyama R, Mizunuma M, Gengyo A, Yamamoto J, Kume K, Miyakawa T, et al. Implication of Ca2+ in the regulation of replicative life span of budding yeast. J Biol Chem. 2011;286:28681-7 pubmed publisher
    ..a mutant of the SIR3 gene, implicated in the regulation of life span, as a suppressor of the Ca(2+) sensitivity of zds1? cells in the budding yeast Saccharomyces cerevisiae...
  17. Searle J, Wood M, Kaur M, Tobin D, Sanchez Y. Proteins in the nutrient-sensing and DNA damage checkpoint pathways cooperate to restrain mitotic progression following DNA damage. PLoS Genet. 2011;7:e1002176 pubmed publisher
    ..Our data indicate that there is cross-talk between the DNA damage checkpoint and the proteins that integrate nutrient and stress signals to regulate PKA. ..
  18. Schwer B, Shuman S. Multicopy suppressors of temperature-sensitive mutations of yeast mRNA capping enzyme. Gene Expr. 1996;5:331-44 pubmed
    We have isolated three Saccharomyces cerevisiae genes-CES1, CES2, and CES3-- that, when present in high copy, suppress the ts growth defect caused by mutations in the CEG1 gene encoding mRNA guanylyltransferase (capping enzyme)...
  19. Balciunas D, Hallberg M, Bjorklund S, Ronne H. Functional interactions within yeast mediator and evidence of differential subunit modifications. J Biol Chem. 2003;278:3831-9 pubmed
    ..Taken together, these results suggest that assembly of Mediator is a multistep process that involves conversion of both Med4 and Med8 to their low mobility forms. ..
  20. Juanes M, Khoueiry R, Kupka T, Castro A, Mudrak I, Ogris E, et al. Budding yeast greatwall and endosulfines control activity and spatial regulation of PP2A(Cdc55) for timely mitotic progression. PLoS Genet. 2013;9:e1003575 pubmed publisher
    ..However, this conserved module is adapted to account for differences in the regulation of mitotic entry in different organisms. ..
  21. Zhang A, Gao W. Mechanisms of protein kinase Sch9 regulating Bcy1 in Saccharomyces cerevisiae. FEMS Microbiol Lett. 2012;331:10-6 pubmed publisher
    ..Our research indicated that Sch9 regulated localization of Bcy1 via Zds1 for the following reasons: (1) deletions of SCH9 or ZDS1 both caused nuclear localization of Bcy1; (2) Sch9 and ..
  22. Rossio V, Kazatskaya A, Hirabayashi M, Yoshida S. Comparative genetic analysis of PP2A-Cdc55 regulators in budding yeast. Cell Cycle. 2014;13:2073-83 pubmed publisher
    ..Sequestration of Cdc55 from the nucleus by Zds1 and Zds2 is important for robust activation of mitotic Cdk1 and mitotic progression in budding yeast...
  23. Mizunuma M, Hirata D, Miyakawa T. Implication of Pkc1p protein kinase C in sustaining Cln2p level and polarized bud growth in response to calcium signaling in Saccharomyces cerevisiae. J Cell Sci. 2005;118:4219-29 pubmed
    ..The Pkc1p novel function was required for posttranscriptional upregulation of Cln2p and appeared to be important for the coordinated regulation of polar bud growth and the cell cycle. ..
  24. Araki T, Uesono Y, Oguchi T, Toh e A. LAS24/KOG1, a component of the TOR complex 1 (TORC1), is needed for resistance to local anesthetic tetracaine and normal distribution of actin cytoskeleton in yeast. Genes Genet Syst. 2005;80:325-43 pubmed
    ..Consistent with the broad cellular functions exerted by the TOR pathway, we found that Las24p was associated with membranes and was localized at vacuoles, the plasma membrane and small vesicles. ..
  25. Nakashima A, Maruki Y, Imamura Y, Kondo C, Kawamata T, Kawanishi I, et al. The yeast Tor signaling pathway is involved in G2/M transition via polo-kinase. PLoS ONE. 2008;3:e2223 pubmed publisher
    ..The C-terminal polo-box domain of Cdc5 has an inhibitory role in nuclear translocation. Taken together, our results indicate a novel function of Tor in the regulation of cell cycle and proliferation. ..
  26. Bourbonnais Y, Faucher N, Pallotta D, Larouche C. Multiple cellular processes affected by the absence of the Rpb4 subunit of RNA polymerase II contribute to the deficiency in the stress response of the yeast rpb4(delta) mutant. Mol Gen Genet. 2001;264:763-72 pubmed
    ..by about 30%) in rpb4 mutants grown in rich medium at moderate temperature, some transcripts, in particular ZDS1, are more abundant...
  27. Thai V, Dephoure N, Weiss A, Ferguson J, Leitao R, Gygi S, et al. Protein Kinase C Controls Binding of Igo/ENSA Proteins to Protein Phosphatase 2A in Budding Yeast. J Biol Chem. 2017;292:4925-4941 pubmed publisher
    ..Together, the data suggest that Pkc1 controls PP2ACdc55 by multiple overlapping mechanisms. ..
  28. Anastasia S, Nguyen D, Thai V, Meloy M, Macdonough T, Kellogg D. A link between mitotic entry and membrane growth suggests a novel model for cell size control. J Cell Biol. 2012;197:89-104 pubmed publisher
    ..Growth-dependent signaling could explain how membrane growth is integrated with cell cycle progression. It could also control both cell size and morphogenesis, thereby reconciling divergent models for mitotic checkpoint function. ..
  29. Sasano Y, Yamagishi K, Tanikawa M, Nakazawa T, Sugiyama M, Kaneko Y, et al. Stabilization of mini-chromosome segregation during mitotic growth by overexpression of YCR041W and its application to chromosome engineering in Saccharomyces cerevisiae. J Biosci Bioeng. 2015;119:526-31 pubmed publisher
    ..Taking these results together, we propose that YCR041W overexpression can be used as a novel chromosome engineering tool for controlling mini-chromosome maintenance and loss. ..
  30. James A, Cocheme H, Murai M, Miyoshi H, Murphy M. Complementation of coenzyme Q-deficient yeast by coenzyme Q analogues requires the isoprenoid side chain. FEBS J. 2010;277:2067-82 pubmed publisher
    ..Here we suggest that CoQ or its redox state may be a signal for growth during the shift to respiration. ..
  31. Rossio V, Michimoto T, Sasaki T, Ohbayashi I, Kikuchi Y, Yoshida S. Nuclear PP2A-Cdc55 prevents APC-Cdc20 activation during the spindle assembly checkpoint. J Cell Sci. 2013;126:4396-405 pubmed publisher
    ..Because the Cdc55-binding proteins Zds1 and Zds2 inhibit both nuclear accumulation of Cdc55 and SAC activity, we propose that spatial control of PP2A by ..
  32. Pierstorff E, Kane C. Genetic interactions between an RNA polymerase II phosphatase and centromeric elements in Saccharomyces cerevisiae. Mol Genet Genomics. 2004;271:603-15 pubmed
    ..For fcp1-2 three second-site suppressors were identified. One contained the ORF for ZDS1. The remaining two suppressors mapped to the centromere regions of chromosomes I and V...