Gene Symbol: YTA12
Description: m-AAA protease subunit YTA12
Alias: RCA1, m-AAA protease subunit YTA12
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Arlt H, Tauer R, Feldmann H, Neupert W, Langer T. The YTA10-12 complex, an AAA protease with chaperone-like activity in the inner membrane of mitochondria. Cell. 1996;85:875-85 pubmed
    ..We propose that proteolytic and chaperone-like activities in the YTA10-12 complex mediate assembly and degradation processes of membrane protein complexes and thereby exert key functions in the maintenance of membrane integrity. ..
  2. Wu X, Li L, Jiang H. Doa1 targets ubiquitinated substrates for mitochondria-associated degradation. J Cell Biol. 2016;213:49-63 pubmed publisher
    ..Collectively, our results demonstrate the essential role of the Doa1-Cdc48(-Ufd1-Npl4)complex in mitochondrial proteostasis and suggest that Doa1 plays dual roles on the Cdc48 complex. ..
  3. Francis B, Thorsness P. Hsp90 and mitochondrial proteases Yme1 and Yta10/12 participate in ATP synthase assembly in Saccharomyces cerevisiae. Mitochondrion. 2011;11:587-600 pubmed publisher
    ..Genetic evidence indicates interaction of Hsc82/Hsp82 with the Yme1 and Yta10/Yta12 complexes in promoting F(1)F(o)-ATPase activity, with Hsc82 specifically required for F(1)-ATPase assembly...
  4. Bestwick M, Khalimonchuk O, Pierrel F, Winge D. The role of Coa2 in hemylation of yeast Cox1 revealed by its genetic interaction with Cox10. Mol Cell Biol. 2010;30:172-85 pubmed publisher
    ..We postulate that the mutant Cox10 complex has enhanced efficiency in the addition of heme a to Cox1. Coa2 appears to impart stability to the oligomeric wild-type Cox10 complex involved in Cox1 hemylation. ..
  5. Pierrel F, Khalimonchuk O, Cobine P, Bestwick M, Winge D. Coa2 is an assembly factor for yeast cytochrome c oxidase biogenesis that facilitates the maturation of Cox1. Mol Cell Biol. 2008;28:4927-39 pubmed publisher
    ..The matrix-localized Coa2 protein may aid in stabilizing an early Cox1 intermediate containing the nuclear subunits Cox5a and Cox6. ..
  6. Augustin S, Gerdes F, Lee S, Tsai F, Langer T, Tatsuta T. An intersubunit signaling network coordinates ATP hydrolysis by m-AAA proteases. Mol Cell. 2009;35:574-85 pubmed publisher
    ..These findings provide insight into how AAA+ proteins convert energy derived from ATP hydrolysis into mechanical work. ..
  7. Rep M, van Dijl J, Suda K, Schatz G, Grivell L, Suzuki C. Promotion of mitochondrial membrane complex assembly by a proteolytically inactive yeast Lon. Science. 1996;274:103-6 pubmed
    ..It is suggested that the mitochondrial proteases Lon, Afg3p, and Rca1p can also serve a chaperone-like function in the assembly of mitochondrial protein complexes. ..
  8. Korbel D, Wurth S, Kaser M, Langer T. Membrane protein turnover by the m-AAA protease in mitochondria depends on the transmembrane domains of its subunits. EMBO Rep. 2004;5:698-703 pubmed
    ..Here, we have analysed the role of the membrane-spanning domains of Yta10 and Yta12, which are conserved subunits of the hetero-oligomeric m-AAA protease in the mitochondria of Saccharomyces ..
  9. Chen X, Moerschell R, Pearce D, Ramanan D, Sherman F. Enhanced mitochondrial degradation of yeast cytochrome c with amphipathic structures. Curr Genet. 2005;47:67-83 pubmed
    ..ADD appeared to be specifically suppressed to various extents by deletions of any of the YME1, AFG3, or RCA1 genes encoding membrane-associated mitochondrial proteases, probably because the amphipathic structures caused a ..

More Information


  1. Suppanz I, Wurm C, Wenzel D, Jakobs S. The m-AAA protease processes cytochrome c peroxidase preferentially at the inner boundary membrane of mitochondria. Mol Biol Cell. 2009;20:572-80 pubmed publisher
    ..These findings suggest that protein quality control and proteolytic activation exerted by the m-AAA protease take place preferentially in the IBM pointing to significant functional differences between the IBM and the CM. ..
  2. Lee S, Lee H, Yoo S, Kim H. Molecular insights into the m-AAA protease-mediated dislocation of transmembrane helices in the mitochondrial inner membrane. J Biol Chem. 2017;292:20058-20066 pubmed publisher
    ..hetero-hexameric AAA (ATPase associated with diverse cellular activities) protease, composed of the Yta10 and Yta12 proteins, regulates mitochondrial proteostasis by mediating protein maturation and degradation...
  3. Tzagoloff A, Yue J, Jang J, Paul M. A new member of a family of ATPases is essential for assembly of mitochondrial respiratory chain and ATP synthetase complexes in Saccharomyces cerevisiae. J Biol Chem. 1994;269:26144-51 pubmed
    ..These results are most compatible with the idea that the gene defined by G25 mutants (RCA1) codes for a protein essential for the assembly of functional respiratory and ATPase complexes...
  4. Cottier F, Raymond M, Kurzai O, Bolstad M, Leewattanapasuk W, Jimenez Lopez C, et al. The bZIP transcription factor Rca1p is a central regulator of a novel CO? sensing pathway in yeast. PLoS Pathog. 2012;8:e1002485 pubmed publisher
    ..Rca1p appears to regulate cellular metabolism in response to CO(2) availability in environments as diverse as the phagosome, yeast communities or liquid culture. ..
  5. Lee S, Augustin S, Tatsuta T, Gerdes F, Langer T, Tsai F. Electron cryomicroscopy structure of a membrane-anchored mitochondrial AAA protease. J Biol Chem. 2011;286:4404-11 pubmed publisher
    ..Here, we have analyzed the hetero-oligomeric yeast m-AAA protease composed of homologous Yta10 and Yta12 subunits...
  6. Nolden M, Ehses S, Koppen M, Bernacchia A, Rugarli E, Langer T. The m-AAA protease defective in hereditary spastic paraplegia controls ribosome assembly in mitochondria. Cell. 2005;123:277-89 pubmed
    ..Our findings therefore rationalize mitochondrial defects associated with m-AAA protease mutants in yeast and shed new light on the mechanism of axonal degeneration in HSP. ..
  7. Tatsuta T, Model K, Langer T. Formation of membrane-bound ring complexes by prohibitins in mitochondria. Mol Biol Cell. 2005;16:248-59 pubmed
    ..Implications of these findings for proposed cellular activities of prohibitins are discussed. ..
  8. Anghileri P, Branduardi P, Sternieri F, Monti P, Visintin R, Bevilacqua A, et al. Chromosome separation and exit from mitosis in budding yeast: dependence on growth revealed by cAMP-mediated inhibition. Exp Cell Res. 1999;250:510-23 pubmed
    ..A possible functional antagonism between PKAs and the mitosis promoting factor is also discussed. ..
  9. Arlt H, Steglich G, Perryman R, Guiard B, Neupert W, Langer T. The formation of respiratory chain complexes in mitochondria is under the proteolytic control of the m-AAA protease. EMBO J. 1998;17:4837-47 pubmed
    ..Deletion of YTA10 or YTA12 impairs degradation of non-assembled inner membrane proteins and assembly of respiratory chain complexes...
  10. Rep M, Nooy J, Guelin E, Grivell L. Three genes for mitochondrial proteins suppress null-mutations in both Afg3 and Rca1 when over-expressed. Curr Genet. 1996;30:206-11 pubmed
    ..All three genes also suppressed a null mutation in a related gene, RCA1, as well as in the combination of afg3- and rca1-null.
  11. Esser K, Tursun B, Ingenhoven M, Michaelis G, Pratje E. A novel two-step mechanism for removal of a mitochondrial signal sequence involves the mAAA complex and the putative rhomboid protease Pcp1. J Mol Biol. 2002;323:835-43 pubmed
    ..The mAAA protease subunits Yta10 (Afg3) and Yta12 (Rca1) were identified both to be essential for the first processing step...
  12. Bonn F, Tatsuta T, Petrungaro C, Riemer J, Langer T. Presequence-dependent folding ensures MrpL32 processing by the m-AAA protease in mitochondria. EMBO J. 2011;30:2545-56 pubmed publisher