YSH1

Summary

Gene Symbol: YSH1
Description: cleavage polyadenylation factor subunit YSH1
Alias: BRR5, cleavage polyadenylation factor subunit YSH1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Vo L, Minet M, Schmitter J, Lacroute F, Wyers F. Mpe1, a zinc knuckle protein, is an essential component of yeast cleavage and polyadenylation factor required for the cleavage and polyadenylation of mRNA. Mol Cell Biol. 2001;21:8346-56 pubmed
    ..These results show that Mpe1p plays a crucial role in 3' end formation probably by promoting the specific link between the CFI/CPF complex and pre-mRNA. ..
  2. Ghazy M, He X, Singh B, Hampsey M, Moore C. The essential N terminus of the Pta1 scaffold protein is required for snoRNA transcription termination and Ssu72 function but is dispensable for pre-mRNA 3'-end processing. Mol Cell Biol. 2009;29:2296-307 pubmed publisher
    ..Furthermore, different regions of Pta1 interact with the CPF subunits Ssu72, Pti1, and Ysh1, supporting the idea that Pta1 acts as a scaffold to organize CPF...
  3. Ohnacker M, Barabino S, Preker P, Keller W. The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex. EMBO J. 2000;19:37-47 pubmed
    ..These results show that Pfs2p plays an essential role in 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex. ..
  4. He X, Khan A, Cheng H, Pappas D, Hampsey M, Moore C. Functional interactions between the transcription and mRNA 3' end processing machineries mediated by Ssu72 and Sub1. Genes Dev. 2003;17:1030-42 pubmed
  5. Kim M, Krogan N, Vasiljeva L, Rando O, Nedea E, Greenblatt J, et al. The yeast Rat1 exonuclease promotes transcription termination by RNA polymerase II. Nature. 2004;432:517-22 pubmed
    ..These findings support a model in which poly(A) site cleavage and subsequent degradation of the 3'-downstream RNA by Rat1 trigger transcription termination. ..
  6. Kyburz A, Sadowski M, Dichtl B, Keller W. The role of the yeast cleavage and polyadenylation factor subunit Ydh1p/Cft2p in pre-mRNA 3'-end formation. Nucleic Acids Res. 2003;31:3936-45 pubmed
  7. Zhao J, Kessler M, Moore C. Cleavage factor II of Saccharomyces cerevisiae contains homologues to subunits of the mammalian Cleavage/ polyadenylation specificity factor and exhibits sequence-specific, ATP-dependent interaction with precursor RNA. J Biol Chem. 1997;272:10831-8 pubmed
    ..The 100-kDa subunit is identical to Brr5/Ysh1, a yeast protein with striking similarity to the 73-kDa subunit of CPSF...
  8. Garas M, Dichtl B, Keller W. The role of the putative 3' end processing endonuclease Ysh1p in mRNA and snoRNA synthesis. RNA. 2008;14:2671-84 pubmed publisher
    ..In addition, we observed a range of additional functional deficiencies in ysh1 mutant strains, which were partially allele-specific...
  9. Dichtl B, Blank D, Ohnacker M, Friedlein A, Roeder D, Langen H, et al. A role for SSU72 in balancing RNA polymerase II transcription elongation and termination. Mol Cell. 2002;10:1139-50 pubmed
    ..The sum of our analyses suggests a negative influence of Ssu72p on RNAP II during transcription that affects the commitment to either elongation or termination. ..

More Information

Publications26

  1. He X, Moore C. Regulation of yeast mRNA 3' end processing by phosphorylation. Mol Cell. 2005;19:619-29 pubmed
    ..These results support a model in which poly(A) synthesis is controlled by cycles of phosphorylation and dephosphorylation that require the action of Glc7. ..
  2. Ryan K, Calvo O, Manley J. Evidence that polyadenylation factor CPSF-73 is the mRNA 3' processing endonuclease. RNA. 2004;10:565-73 pubmed
    ..Taken together, the available data provide strong evidence that CPSF-73 is the 3' processing endonuclease. ..
  3. Preker P, Ohnacker M, Minvielle Sebastia L, Keller W. A multisubunit 3' end processing factor from yeast containing poly(A) polymerase and homologues of the subunits of mammalian cleavage and polyadenylation specificity factor. EMBO J. 1997;16:4727-37 pubmed
    ..PF I also appears to be functionally related to CPSF, as it polyadenylates a substrate RNA more efficiently than Pap1p alone. Possibly, the observed interaction of the complex with RNA tethers Pap1p to its substrate. ..
  4. Zhelkovsky A, Tacahashi Y, Nasser T, He X, Sterzer U, Jensen T, et al. The role of the Brr5/Ysh1 C-terminal domain and its homolog Syc1 in mRNA 3'-end processing in Saccharomyces cerevisiae. RNA. 2006;12:435-45 pubmed
    ..Because of similarity to other nucleases in the metallo-beta-lactamase family, the Brr5/Ysh1 subunit has been proposed to be the endonuclease...
  5. Nedea E, He X, Kim M, Pootoolal J, Zhong G, Canadien V, et al. Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends. J Biol Chem. 2003;278:33000-10 pubmed
    ..Ref2 and Pta1 similarly affect at least one snoRNA transcript. ..
  6. Zhao J, Kessler M, Helmling S, O Connor J, Moore C. Pta1, a component of yeast CF II, is required for both cleavage and poly(A) addition of mRNA precursor. Mol Cell Biol. 1999;19:7733-40 pubmed
    ..The three largest subunits, Cft1/Yhh1, Cft2/Ydh1, and Brr5/Ysh1, are homologs of the three largest subunits of mammalian cleavage-polyadenylation specificity factor (CPSF), an ..
  7. Dheur S, Nykamp K, Viphakone N, Swanson M, Minvielle Sebastia L. Yeast mRNA Poly(A) tail length control can be reconstituted in vitro in the absence of Pab1p-dependent Poly(A) nuclease activity. J Biol Chem. 2005;280:24532-8 pubmed
    ..Based on these findings, we propose that Nab2p is necessary and sufficient to regulate poly(A) tail length during de novo synthesis in yeast. ..
  8. Tacahashi Y, Helmling S, Moore C. Functional dissection of the zinc finger and flanking domains of the Yth1 cleavage/polyadenylation factor. Nucleic Acids Res. 2003;31:1744-52 pubmed
    ..Yth1 was previously shown to interact with pre-mRNA and with two CPF subunits, Brr5/Ysh1 and the polyadenylation-specific Fip1, and to act in both steps of mRNA 3' end processing...
  9. Roguev A, Schaft D, Shevchenko A, Pijnappel W, Wilm M, Aasland R, et al. The Saccharomyces cerevisiae Set1 complex includes an Ash2 homologue and methylates histone 3 lysine 4. EMBO J. 2001;20:7137-48 pubmed
    ..We propose that eukaryotic Set1Cs are H3 lysine 4 methyltransferases and are related to trxG action through association with Ash2 homologues. ..
  10. Ezeokonkwo C, Zhelkovsky A, Lee R, Bohm A, Moore C. A flexible linker region in Fip1 is needed for efficient mRNA polyadenylation. RNA. 2011;17:652-64 pubmed publisher
    ..Our results indicate that the Fip1 linker, through its flexibility and protein/protein interactions, allows Pap1 to reach the 3' end of the cleaved RNA and efficiently initiate poly(A) addition. ..
  11. Holbein S, Scola S, Loll B, Dichtl B, Hubner W, Meinhart A, et al. The P-loop domain of yeast Clp1 mediates interactions between CF IA and CPF factors in pre-mRNA 3' end formation. PLoS ONE. 2011;6:e29139 pubmed publisher
    ..In contrast, mutations in Clp1 enhanced binding to the 3' endonuclease Ysh1 that is a component of CPF. Our results support a structural role for the Clp1 P-loop motif...
  12. Schreieck A, Easter A, Etzold S, Wiederhold K, Lidschreiber M, Cramer P, et al. RNA polymerase II termination involves C-terminal-domain tyrosine dephosphorylation by CPF subunit Glc7. Nat Struct Mol Biol. 2014;21:175-179 pubmed publisher
    ..These results show that transcription termination involves Tyr1 dephosphorylation of the CTD and indicate that pre-mRNA processing by CPF and transcription termination are coupled via Glc7-dependent Pol II-Tyr1 dephosphorylation. ..
  13. Yang F, Hsu P, Lee S, Yang W, Hoskinson D, Xu W, et al. The C terminus of Pcf11 forms a novel zinc-finger structure that plays an essential role in mRNA 3'-end processing. RNA. 2017;23:98-107 pubmed
    ..These findings provide further insight into this important protein and its multiple functional roles during cotranscriptional RNA processing. ..
  14. Wohlschlegel J, Johnson E, Reed S, Yates J. Global analysis of protein sumoylation in Saccharomyces cerevisiae. J Biol Chem. 2004;279:45662-8 pubmed
    ..Additionally, our global analysis has revealed a number of interesting biological patterns in the list of SUMO targets including a clustering of sumoylation targets within macromolecular complexes. ..
  15. Ghazy M, Gordon J, Lee S, Singh B, Bohm A, Hampsey M, et al. The interaction of Pcf11 and Clp1 is needed for mRNA 3'-end formation and is modulated by amino acids in the ATP-binding site. Nucleic Acids Res. 2012;40:1214-25 pubmed publisher
    ..These results define Clp1 as a bridge between CF IA and CPF and indicate that the Clp1-Pcf11 interaction is modulated by amino acids in the conserved ATP-binding site of Clp1. ..
  16. Lee S, Moore C. Efficient mRNA polyadenylation requires a ubiquitin-like domain, a zinc knuckle, and a RING finger domain, all contained in the Mpe1 protein. Mol Cell Biol. 2014;34:3955-67 pubmed publisher
    ..Furthermore, an inhibitor of ubiquitin-mediated interactions blocks cleavage, demonstrating for the first time a direct role for ubiquitination in mRNA 3'-end processing. ..
  17. Zhang D, Mosley A, Ramisetty S, Rodríguez Molina J, Washburn M, Ansari A. Ssu72 phosphatase-dependent erasure of phospho-Ser7 marks on the RNA polymerase II C-terminal domain is essential for viability and transcription termination. J Biol Chem. 2012;287:8541-51 pubmed publisher
    ..An inability to remove these marks prevents Pol II from terminating efficiently and will likely impede subsequent assembly into the pre-initiation complex. ..