Gene Symbol: YPT6
Description: Rab family GTPase YPT6
Alias: Rab family GTPase YPT6
Species: Saccharomyces cerevisiae S288c
Products:     YPT6

Top Publications

  1. Plemel R, Lobingier B, Brett C, Angers C, Nickerson D, Paulsel A, et al. Subunit organization and Rab interactions of Vps-C protein complexes that control endolysosomal membrane traffic. Mol Biol Cell. 2011;22:1353-63 pubmed publisher
    ..Biochemical and genetic experiments demonstrate the importance of these regions, revealing the Vps11 CTD as a key integrator of Vps-C complex assembly, Rab signaling, and endosomal and lysosomal traffic. ..
  2. Tsukada M, Gallwitz D. Isolation and characterization of SYS genes from yeast, multicopy suppressors of the functional loss of the transport GTPase Ypt6p. J Cell Sci. 1996;109 ( Pt 10):2471-81 pubmed
    In Saccharomyces cerevisiae, the YPT6 gene encodes the homologue of the mammalian Rab6 protein found in the Golgi apparatus...
  3. Tsukada M, Will E, Gallwitz D. Structural and functional analysis of a novel coiled-coil protein involved in Ypt6 GTPase-regulated protein transport in yeast. Mol Biol Cell. 1999;10:63-75 pubmed
    ..Its loss of function results in partial missorting of carboxypeptidase Y. Double disruptions of SYS3 and YPT6 lead to a significant growth inhibition of the mutant cells, to a massive accumulation of 40- to 50-nm vesicles, to ..
  4. Bensen E, Yeung B, Payne G. Ric1p and the Ypt6p GTPase function in a common pathway required for localization of trans-Golgi network membrane proteins. Mol Biol Cell. 2001;12:13-26 pubmed
    ..SYS1, originally discovered as a multicopy suppressor of defects caused by the absence of the Rab GTPase YPT6, was identified as a multicopy suppressor of ric1Delta...
  5. Li B, Warner J. Mutation of the Rab6 homologue of Saccharomyces cerevisiae, YPT6, inhibits both early Golgi function and ribosome biosynthesis. J Biol Chem. 1996;271:16813-9 pubmed
    ..product that is nearly 65% identical to the human GTPase, Rab6, and is likely to be identical to the yeast gene YPT6. It is essential for growth only at elevated temperatures...
  6. Setty S, Strochlic T, Tong A, Boone C, Burd C. Golgi targeting of ARF-like GTPase Arl3p requires its Nalpha-acetylation and the integral membrane protein Sys1p. Nat Cell Biol. 2004;6:414-9 pubmed
    ..Chemical crosslinking and fluorescence microscopy experiments demonstrate that localization of Arl3p also requires Sys1p, a Golgi-localized integral membrane protein, which may serve as a receptor for acetylated Arl3p. ..
  7. Luo Z, Gallwitz D. Biochemical and genetic evidence for the involvement of yeast Ypt6-GTPase in protein retrieval to different Golgi compartments. J Biol Chem. 2003;278:791-9 pubmed
    ..Based on previous studies with ypt6 deletion mutants, a regulatory role of the GTPase either in protein retrieval to the trans-Golgi network or in ..
  8. Siniossoglou S, Pelham H. An effector of Ypt6p binds the SNARE Tlg1p and mediates selective fusion of vesicles with late Golgi membranes. EMBO J. 2001;20:5991-8 pubmed
    ..We suggest that the VFT complex links vesicles containing Tlg1p to their target, which is defined by the local activation of Ypt6p. ..
  9. Siniossoglou S, Peak Chew S, Pelham H. Ric1p and Rgp1p form a complex that catalyses nucleotide exchange on Ypt6p. EMBO J. 2000;19:4885-94 pubmed
    ..A screen for mutants that, like ypt6, specifically impair the growth of imh1 cells led to the identification of RIC1...

More Information


  1. Calero M, Collins R. Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins. Biochem Biophys Res Commun. 2002;290:676-81 pubmed
    ..The interactions between Pra1p/Yip3p and Rab proteins are dependent on the presence of the Rab protein C-terminal cysteines and require C-terminal prenylation. ..
  2. Li B, Warner J. Genetic interaction between YPT6 and YPT1 in Saccharomyces cerevisiae. Yeast. 1998;14:915-22 pubmed
    ..Here we show that overexpression of YPT1 suppresses the growth and secretion defects of a ypt6 temperature-sensitive (ts) strain...
  3. Will E, Albert S, Gallwitz D. Expression, purification, and biochemical properties of Ypt/Rab GTPase-activating proteins of Gyp family. Methods Enzymol. 2001;329:50-8 pubmed
  4. Gendre D, Oh J, Boutté Y, Best J, Samuels L, Nilsson R, et al. Conserved Arabidopsis ECHIDNA protein mediates trans-Golgi-network trafficking and cell elongation. Proc Natl Acad Sci U S A. 2011;108:8048-53 pubmed publisher
    ..Hence, ECH is an evolutionarily conserved component of the TGN with a central role in TGN structure and function. ..
  5. Ali R, Brett C, Mukherjee S, Rao R. Inhibition of sodium/proton exchange by a Rab-GTPase-activating protein regulates endosomal traffic in yeast. J Biol Chem. 2004;279:4498-506 pubmed
    ..Taken together with its GTPase-activating protein-dependent role as a negative regulator of Ypt6-mediated retrograde traffic to the Golgi, we propose that Gyp6 coordinates upstream and downstream events in the ..
  6. Fridmann Sirkis Y, Siniossoglou S, Pelham H. TMF is a golgin that binds Rab6 and influences Golgi morphology. BMC Cell Biol. 2004;5:18 pubmed
    ..We show here that the yeast protein Sgm1, previously shown to be recruited to the Golgi by the GTPase Ypt6, binds to Ypt6:GTP via a conserved 100-residue coiled-coil motif that can be identified in a wide range of ..
  7. Ohashi Y, Munro S. Membrane delivery to the yeast autophagosome from the Golgi-endosomal system. Mol Biol Cell. 2010;21:3998-4008 pubmed publisher
    ..This is underestimated by examination of single mutants, providing a possible explanation for discrepancies between yeast and mammalian studies on Atg9 localization and autophagosome formation. ..
  8. Paulsel A, Merz A, Nickerson D. Vps9 family protein Muk1 is the second Rab5 guanosine nucleotide exchange factor in budding yeast. J Biol Chem. 2013;288:18162-71 pubmed publisher
    ..Microscopy and overexpression experiments demonstrate that Vps9 and Muk1 have distinct localization determinants. These experiments establish Muk1 as the second Rab5 GEF in budding yeast. ..
  9. Kishimoto T, Yamamoto T, Tanaka K. Defects in structural integrity of ergosterol and the Cdc50p-Drs2p putative phospholipid translocase cause accumulation of endocytic membranes, onto which actin patches are assembled in yeast. Mol Biol Cell. 2005;16:5592-609 pubmed
    ..Simultaneous loss of both phospholipid asymmetry and sterol structural integrity could lead to accumulation of endocytic intermediates capable of initiating assembly of actin patches in the cytoplasm. ..
  10. Hsu J, Chang L, Jang L, Huang C, Lee F. The N-terminus of Vps74p is essential for the retention of glycosyltransferases in the Golgi but not for the modulation of apical polarized growth in Saccharomyces cerevisiae. PLoS ONE. 2013;8:e74715 pubmed publisher
    ..Thus, we propose that Vps74p may use different domains to interact with specific effectors thereby differentially modulating a variety of cellular functions. ..
  11. Lafourcade C, Galan J, Gloor Y, Haguenauer Tsapis R, Peter M. The GTPase-activating enzyme Gyp1p is required for recycling of internalized membrane material by inactivation of the Rab/Ypt GTPase Ypt1p. Mol Cell Biol. 2004;24:3815-26 pubmed
    ..Interestingly, the deletions of GYP1 and YPT6 were synthetic lethal, raising the possibility that at least two distinct pathways are involved in recycling of ..
  12. Strom M, Vollmer P, Tan T, Gallwitz D. A yeast GTPase-activating protein that interacts specifically with a member of the Ypt/Rab family. Nature. 1993;361:736-9 pubmed
    ..We report here the first cloned GTPase-activating protein for the Ypt/Rab protein family. The gene, GYP6 (GAP of Ypt6 protein), encodes a protein of 458 amino acids which is highly specific for the Ypt6 protein and shows little or no ..
  13. Webster M, McCaffery J, COHEN FIX O. Vesicle trafficking maintains nuclear shape in Saccharomyces cerevisiae during membrane proliferation. J Cell Biol. 2010;191:1079-88 pubmed publisher
    ..Moreover, flares may facilitate maintenance of a constant nuclear/cell volume ratio in the face of altered membrane proliferation. ..
  14. Georgiev A, Leipus A, Olsson I, Berrez J, Mutvei A. Characterization of MYR1, a dosage suppressor of YPT6 and RIC1 deficient mutants. Curr Genet. 2008;53:235-47 pubmed publisher
    ..One member of this family is the Saccharomyces cerevisae protein Ypt6. To search for new genes interacting with Ypt6-related pathways, we performed a genetic screen for high copy ..
  15. Chen C, Collins R. Insights into biological functions across species: examining the role of Rab proteins in YIP1 family function. Biochem Soc Trans. 2005;33:614-8 pubmed
    ..e. independent or dependent activities of Yip1p. ..
  16. Calero M, Chen C, Zhu W, Winand N, Havas K, Gilbert P, et al. Dual prenylation is required for Rab protein localization and function. Mol Biol Cell. 2003;14:1852-67 pubmed
  17. Suvorova E, Duden R, Lupashin V. The Sec34/Sec35p complex, a Ypt1p effector required for retrograde intra-Golgi trafficking, interacts with Golgi SNAREs and COPI vesicle coat proteins. J Cell Biol. 2002;157:631-43 pubmed
    ..We propose that the Sec34/35 protein complex acts as a tether that connects cis-Golgi membranes and COPI-coated, retrogradely targeted intra-Golgi vesicles. ..
  18. Lazar T, Gotte M, Gallwitz D. Vesicular transport: how many Ypt/Rab-GTPases make a eukaryotic cell?. Trends Biochem Sci. 1997;22:468-72 pubmed
    ..There is some redundancy among the 11 Ypt proteins, and only those involved in the biosynthetic pathway are essential for cell viability. ..
  19. Maresova L, Vydarený T, Sychrova H. Comparison of the influence of small GTPases Arl1 and Ypt6 on yeast cells' tolerance to various stress factors. FEMS Yeast Res. 2012;12:332-40 pubmed publisher
    The GTPases Arl1 and Ypt6 are involved in the intracellular transport of vesicles and their fusion with the trans-Golgi network...
  20. Albert S, Gallwitz D. Two new members of a family of Ypt/Rab GTPase activating proteins. Promiscuity of substrate recognition. J Biol Chem. 1999;274:33186-9 pubmed
    ..The results demonstrate that in yeast there is a large family of Ypt/Rab-GAPs, members of which discriminate poorly between GTPases involved in regulating different steps of exo- and endocytic transport routes. ..
  21. Yang S, Rosenwald A. Autophagy in Saccharomyces cerevisiae requires the monomeric GTP-binding proteins, Arl1 and Ypt6. Autophagy. 2016;12:1721-1737 pubmed
    ..Here we demonstrate that 2 monomeric GTP-binding proteins in Saccharomyces cerevisiae, Arl1 and Ypt6, which belong to the Arf/Arl/Sar protein family and the Rab family, respectively, and control endosome-trans-Golgi ..
  22. Soper J, Kehm V, Burd C, Bankaitis V, Lee V. Aggregation of ?-synuclein in S. cerevisiae is associated with defects in endosomal trafficking and phospholipid biosynthesis. J Mol Neurosci. 2011;43:391-405 pubmed publisher
    ..Significantly, we demonstrate that ?-synuclein expression induces vulnerability to perturbations of Ypt6 and other proteins involved in retrograde endosome-Golgi transport, linking a specific trafficking defect to ?-..
  23. Calero M, Whittaker G, Collins R. Yop1p, the yeast homolog of the polyposis locus protein 1, interacts with Yip1p and negatively regulates cell growth. J Biol Chem. 2001;276:12100-12 pubmed
    ..These results suggest that Yop1p acts in conjunction with Yip1p to mediate a common step in membrane traffic. ..
  24. Suda Y, Kurokawa K, Hirata R, Nakano A. Rab GAP cascade regulates dynamics of Ypt6 in the Golgi traffic. Proc Natl Acad Sci U S A. 2013;110:18976-81 pubmed publisher
    ..Here, we report the dynamics of Ypt6, a yeast member of the Rab GTPase family, which mediates the fusion of vesicles from endosomes at the Golgi ..
  25. Kawamura S, Nagano M, Toshima J, Toshima J. Analysis of subcellular localization and function of the yeast Rab6 homologue, Ypt6p, using a novel amino-terminal tagging strategy. Biochem Biophys Res Commun. 2014;450:519-25 pubmed publisher
    ..These results suggest that the GTP-binding activity of Ypt6p is necessary for intra-Golgi trafficking and protein recycling in the early Golgi compartment. ..
  26. Yang S, Rosenwald A. A High Copy Suppressor Screen for Autophagy Defects in Saccharomyces arl1? and ypt6? Strains. G3 (Bethesda). 2017;7:333-341 pubmed publisher
    In Saccharomyces cerevisiae, Arl1 and Ypt6, two small GTP-binding proteins that regulate membrane traffic in the secretory and endocytic pathways, are also necessary for autophagy...
  27. Lauwers E, Jacob C, Andre B. K63-linked ubiquitin chains as a specific signal for protein sorting into the multivesicular body pathway. J Cell Biol. 2009;185:493-502 pubmed publisher
    ..Our data reveal that K63-linked Ub chains act as a specific signal for MVB sorting, providing further insight into the Ub code of membrane protein trafficking. ..
  28. Kurischko C, Kuravi V, Wannissorn N, Nazarov P, Husain M, Zhang C, et al. The yeast LATS/Ndr kinase Cbk1 regulates growth via Golgi-dependent glycosylation and secretion. Mol Biol Cell. 2008;19:5559-78 pubmed publisher
    ..These data suggest that an important function for Cbk1 and the RAM signaling network is to regulate growth and secretion via Golgi and Sec2/Sec4-dependent processes. ..
  29. Inadome H, Noda Y, Kamimura Y, Adachi H, Yoda K. Tvp38, Tvp23, Tvp18 and Tvp15: novel membrane proteins in the Tlg2-containing Golgi/endosome compartments of Saccharomyces cerevisiae. Exp Cell Res. 2007;313:688-97 pubmed
    ..Disruptions of tvp15 and tvp23 showed synthetic aggravation with ypt6 or ric1 null mutation...
  30. Chen C, Calero M, DeRegis C, Heidtman M, Barlowe C, Collins R. Genetic analysis of yeast Yip1p function reveals a requirement for Golgi-localized rab proteins and rab-Guanine nucleotide dissociation inhibitor. Genetics. 2004;168:1827-41 pubmed
  31. Will E, Gallwitz D. Biochemical characterization of Gyp6p, a Ypt/Rab-specific GTPase-activating protein from yeast. J Biol Chem. 2001;276:12135-9 pubmed
    ..Physical interaction of the catalytically inactive Gyp6(R155A) mutant GAP with Ypt6 wild-type and Ypt6 mutant proteins could be demonstrated with the two-hybrid system...
  32. Li Y, Schmitt H, Gallwitz D, Peng R. Mutations of the SM protein Sly1 resulting in bypass of GTPase requirement in vesicular transport are confined to a short helical region. FEBS Lett. 2007;581:5698-702 pubmed
    ..Here, we describe new mutants of the yeast SM protein Sly1 that specifically bypass the need for GTPases Ypt1 and Ypt6 in vesicular transport...
  33. Benjamin J, Poon P, Drysdale J, Wang X, Singer R, Johnston G. Dysregulated Arl1, a regulator of post-Golgi vesicle tethering, can inhibit endosomal transport and cell proliferation in yeast. Mol Biol Cell. 2011;22:2337-47 pubmed publisher
    ..We show that gene deletions affecting the Arl1 or Ypt6 vesicle-tethering pathways prevent Arl1 activation and membrane localization, and restore growth and trafficking in ..
  34. Guo J, Polymenis M. Dcr2 targets Ire1 and downregulates the unfolded protein response in Saccharomyces cerevisiae. EMBO Rep. 2006;7:1124-7 pubmed
    ..Our results are consistent with de-phosphorylation of Ire1 being a mechanism for antagonizing UPR signalling. ..
  35. Siniossoglou S. Affinity purification of Ypt6 effectors and identification of TMF/ARA160 as a Rab6 interactor. Methods Enzymol. 2005;403:599-607 pubmed
    ..In budding yeast Ypt6 is required for recycling of membrane proteins from endosomes back to the Golgi...
  36. Upadhya R, Lee J, Willis I. Maf1 is an essential mediator of diverse signals that repress RNA polymerase III transcription. Mol Cell. 2002;10:1489-94 pubmed
    ..Biochemical studies identified the initiation factor TFIIIB as a target of Maf1-dependent repression and revealed a defect in TFIIIB-DNA complex assembly under repressing conditions. ..
  37. Calero M, Winand N, Collins R. Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors. FEBS Lett. 2002;515:89-98 pubmed
  38. Albert S, Gallwitz D. Msb4p, a protein involved in Cdc42p-dependent organization of the actin cytoskeleton, is a Ypt/Rab-specific GAP. Biol Chem. 2000;381:453-6 pubmed
    ..Purified Msb4p/Gyp4p acts primarily on Sec4p, Ypt6p and Ypt7p and might have a role in polarized secretion. ..
  39. Lo S, Brett C, Plemel R, Vignali M, Fields S, Gonen T, et al. Intrinsic tethering activity of endosomal Rab proteins. Nat Struct Mol Biol. 2011;19:40-7 pubmed publisher
    ..In our working model, the intrinsic tethering capacity Vps21 operates in concert with conventional effectors and SNAREs to drive efficient docking and fusion. ..
  40. Lukehart J, Highfill C, Kim K. Vps1, a recycling factor for the traffic from early endosome to the late Golgi. Biochem Cell Biol. 2013;91:455-65 pubmed publisher