YPT52

Summary

Gene Symbol: YPT52
Description: Rab family GTPase YPT52
Alias: Rab family GTPase YPT52
Species: Saccharomyces cerevisiae S288c
Products:     YPT52

Top Publications

  1. Singer Krüger B, Stenmark H, Düsterhöft A, Philippsen P, Yoo J, Gallwitz D, et al. Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast. J Cell Biol. 1994;125:283-98 pubmed
    ..Sequencing of the yeast chromosome XI has revealed a third rab5-like gene, YPT52, whose protein product exhibits a similar identity to rab5 and the other two YPT gene products...
  2. Plemel R, Lobingier B, Brett C, Angers C, Nickerson D, Paulsel A, et al. Subunit organization and Rab interactions of Vps-C protein complexes that control endolysosomal membrane traffic. Mol Biol Cell. 2011;22:1353-63 pubmed publisher
    ..Biochemical and genetic experiments demonstrate the importance of these regions, revealing the Vps11 CTD as a key integrator of Vps-C complex assembly, Rab signaling, and endosomal and lysosomal traffic. ..
  3. Pawelec A, Arsić J, Kölling R. Mapping of Vps21 and HOPS binding sites in Vps8 and effect of binding site mutants on endocytic trafficking. Eukaryot Cell. 2010;9:602-10 pubmed publisher
    ..In contrast, deletions that abolished Vps21 binding showed only a modest effect. This suggests that the Vps21 interaction is not essential for endosomal trafficking but may be important for some other aspect of Vps8 function. ..
  4. Paulsel A, Merz A, Nickerson D. Vps9 family protein Muk1 is the second Rab5 guanosine nucleotide exchange factor in budding yeast. J Biol Chem. 2013;288:18162-71 pubmed publisher
    ..trafficking defects considerably less severe than multiple deletions of the three cognate Rab5 paralogs (Vps21, Ypt52, and Ypt53)...
  5. Toshima J, Nishinoaki S, Sato Y, Yamamoto W, Furukawa D, Siekhaus D, et al. Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole. Nat Commun. 2014;5:3498 pubmed publisher
    ..We propose that the endocytic traffic branches into two routes to reach the vacuole: a Rab5-dependent VPS pathway and a Rab5-independent AP-3-mediated pathway. ..
  6. Nickerson D, Russell M, Lo S, Chapin H, Milnes J, Merz A. Termination of isoform-selective Vps21/Rab5 signaling at endolysosomal organelles by Msb3/Gyp3. Traffic. 2012;13:1411-1428 pubmed publisher
    ..Like humans, Saccharomyces cerevisiae has three Rab5s (Vps21, Ypt52 and Ypt53) and one Rab7 (Ypt7). Here, we elucidate the functional roles and regulation of the yeast Rab5s...
  7. Zanolari B, Rockenbauch U, Trautwein M, Clay L, Barral Y, Spang A. Transport to the plasma membrane is regulated differently early and late in the cell cycle in Saccharomyces cerevisiae. J Cell Sci. 2011;124:1055-66 pubmed publisher
    ..We propose that transport to and from the bud neck is regulated differently in small- and large-budded cells, and differs early and late in the cell cycle. ..
  8. Calero M, Winand N, Collins R. Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors. FEBS Lett. 2002;515:89-98 pubmed
  9. Lo S, Brett C, Plemel R, Vignali M, Fields S, Gonen T, et al. Intrinsic tethering activity of endosomal Rab proteins. Nat Struct Mol Biol. 2011;19:40-7 pubmed publisher
    ..In our working model, the intrinsic tethering capacity Vps21 operates in concert with conventional effectors and SNAREs to drive efficient docking and fusion. ..

More Information

Publications20

  1. Calero M, Chen C, Zhu W, Winand N, Havas K, Gilbert P, et al. Dual prenylation is required for Rab protein localization and function. Mol Biol Cell. 2003;14:1852-67 pubmed
  2. Lachmann J, Barr F, Ungermann C. The Msb3/Gyp3 GAP controls the activity of the Rab GTPases Vps21 and Ypt7 at endosomes and vacuoles. Mol Biol Cell. 2012;23:2516-26 pubmed publisher
    ..Our data indicate that GAPs such as Msb3 can act on multiple substrates in vivo at both ends of a trafficking pathway. This ensures specificity of the subsequent GEF-mediated activation of the Rab that initiates the next transport event...
  3. Will E, Albert S, Gallwitz D. Expression, purification, and biochemical properties of Ypt/Rab GTPase-activating proteins of Gyp family. Methods Enzymol. 2001;329:50-8 pubmed
  4. Chen C, Collins R. Insights into biological functions across species: examining the role of Rab proteins in YIP1 family function. Biochem Soc Trans. 2005;33:614-8 pubmed
    ..e. independent or dependent activities of Yip1p. ..
  5. Liu Y, Nakatsukasa K, Kotera M, Kanada A, Nishimura T, Kishi T, et al. Non-SCF-type F-box protein Roy1/Ymr258c interacts with a Rab5-like GTPase Ypt52 and inhibits Ypt52 function. Mol Biol Cell. 2011;22:1575-84 pubmed publisher
    ..Vps21/Ypt51 is a key regulator of intracellular transportation; however, deletion of its isoforms, Ypt52 and Ypt53, results in only a modest inhibition of intracellular trafficking...
  6. Cho J, Noda Y, Yoda K. Proteins in the early golgi compartment of Saccharomyces cerevisiae immunoisolated by Sed5p. FEBS Lett. 2000;469:151-4 pubmed
  7. Prescianotto Baschong C, Riezman H. Ordering of compartments in the yeast endocytic pathway. Traffic. 2002;3:37-49 pubmed
    ..On the other hand, traffic to the early recycling endosome is less dependent on Rab5 homologs and does not require Vps27p. ..
  8. Calero M, Collins R. Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins. Biochem Biophys Res Commun. 2002;290:676-81 pubmed
    ..The interactions between Pra1p/Yip3p and Rab proteins are dependent on the presence of the Rab protein C-terminal cysteines and require C-terminal prenylation. ..
  9. Cabrera M, Arlt H, Epp N, Lachmann J, Griffith J, Perz A, et al. Functional separation of endosomal fusion factors and the class C core vacuole/endosome tethering (CORVET) complex in endosome biogenesis. J Biol Chem. 2013;288:5166-75 pubmed publisher
    ..Importantly, CORVET localization requires the Rab5 homologs Vps21 and Ypt52, whereas Vac1 localization is strictly Vps21-dependent...
  10. Fuchs U, Hause G, Schuchardt I, Steinberg G. Endocytosis is essential for pathogenic development in the corn smut fungus Ustilago maydis. Plant Cell. 2006;18:2066-81 pubmed
    ..Thus, we conclude that endocytosis is essential for recognition of the partner at the beginning of the pathogenic program but has additional roles in mating as well as spore formation and germination. ..
  11. Calero M, Whittaker G, Collins R. Yop1p, the yeast homolog of the polyposis locus protein 1, interacts with Yip1p and negatively regulates cell growth. J Biol Chem. 2001;276:12100-12 pubmed
    ..These results suggest that Yop1p acts in conjunction with Yip1p to mediate a common step in membrane traffic. ..