Gene Symbol: YPT31
Description: Rab family GTPase YPT31
Alias: YPT8, Rab family GTPase YPT31
Species: Saccharomyces cerevisiae S288c
Products:     YPT31

Top Publications

  1. Benli M, Doring F, Robinson D, Yang X, Gallwitz D. Two GTPase isoforms, Ypt31p and Ypt32p, are essential for Golgi function in yeast. EMBO J. 1996;15:6460-75 pubmed
    ..Here we report on the isolation and characterization of two genes (YPT31 and YPT32) of the yeast Saccharomyces cerevisiae which encode members of the Ypt family exhibiting >80% ..
  2. Jedd G, Mulholland J, Segev N. Two new Ypt GTPases are required for exit from the yeast trans-Golgi compartment. J Cell Biol. 1997;137:563-80 pubmed
    ..Yeast cells can tolerate deletion of either the YPT31 or the YPT32 gene, but not both...
  3. Yoo J, Grabowski R, Xing L, Trepte H, Schmitt H, Gallwitz D. Functional implications of genetic interactions between genes encoding small GTPases involved in vesicular transport in yeast. Mol Gen Genet. 1999;261:80-91 pubmed
    ..In yeast, Ypt1p has an essential function late in endoplasmic reticulum (ER) to Golgi transport, and the redundant Ypt31/Ypt32 GTPases have been proposed to act in transport through and/or from the Golgi...
  4. Jones S, Jedd G, Kahn R, Franzusoff A, Bartolini F, Segev N. Genetic interactions in yeast between Ypt GTPases and Arf guanine nucleotide exchangers. Genetics. 1999;152:1543-56 pubmed
    ..Recently, we have shown a role for Ypt31/32p in exit from the yeast trans-Golgi, suggesting a possible function for Ypt/rab proteins in vesicle budding as ..
  5. Ortiz D, Medkova M, Walch Solimena C, Novick P. Ypt32 recruits the Sec4p guanine nucleotide exchange factor, Sec2p, to secretory vesicles; evidence for a Rab cascade in yeast. J Cell Biol. 2002;157:1005-15 pubmed
    ..Here we present evidence that Ypt31/32p, members of Rab family of GTPases, regulate Sec2p function. First, YPT31/YPT32 suppress the sec2-78 mutation...
  6. Chen S, Chen S, Tokarev A, Liu F, Jedd G, Segev N. Ypt31/32 GTPases and their novel F-box effector protein Rcy1 regulate protein recycling. Mol Biol Cell. 2005;16:178-92 pubmed
    ..F-box protein Rcy1, which mediates plasma membrane recycling, is identified here as a downstream effector of the Ypt31/32 GTPase pair because it binds active GTP-bound Ypt31/32 and colocalizes with these GTPases on late Golgi and ..
  7. Furuta N, Fujimura Kamada K, Saito K, Yamamoto T, Tanaka K. Endocytic recycling in yeast is regulated by putative phospholipid translocases and the Ypt31p/32p-Rcy1p pathway. Mol Biol Cell. 2007;18:295-312 pubmed
    ..Screening for multicopy suppressors of cdc50-ts identified YPT31/32, two genes that encode Rab family small GTPases that are involved in both the exocytic and endocytic recycling ..
  8. Chen C, Calero M, DeRegis C, Heidtman M, Barlowe C, Collins R. Genetic analysis of yeast Yip1p function reveals a requirement for Golgi-localized rab proteins and rab-Guanine nucleotide dissociation inhibitor. Genetics. 2004;168:1827-41 pubmed
  9. Yamamoto K, Jigami Y. Mutation of TRS130, which encodes a component of the TRAPP II complex, activates transcription of OCH1 in Saccharomyces cerevisiae. Curr Genet. 2002;42:85-93 pubmed
    ..b>YPT31 and YPT32, the multi-copy suppressors of the trs130(ts1) mutant, differed in the extent of suppression of the ..

More Information


  1. Demmel L, Beck M, Klose C, Schlaitz A, Gloor Y, Hsu P, et al. Nucleocytoplasmic shuttling of the Golgi phosphatidylinositol 4-kinase Pik1 is regulated by 14-3-3 proteins and coordinates Golgi function with cell growth. Mol Biol Cell. 2008;19:1046-61 pubmed publisher
    ..These data suggest a role of Pik1p nucleocytoplasmic shuttling in coordination of biosynthetic transport from the Golgi with nutrient signaling. ..
  2. Calero M, Winand N, Collins R. Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors. FEBS Lett. 2002;515:89-98 pubmed
  3. Mizuno Yamasaki E, Medkova M, Coleman J, Novick P. Phosphatidylinositol 4-phosphate controls both membrane recruitment and a regulatory switch of the Rab GEF Sec2p. Dev Cell. 2010;18:828-40 pubmed publisher
    ..In this way, the regulation of PI4P levels may switch Sec2p/Sec4p function during vesicle maturation, from a Rab GEF recruitment cascade involving Ypt32p to an effector positive feedback loop involving Sec15p. ..
  4. Lo S, Brett C, Plemel R, Vignali M, Fields S, Gonen T, et al. Intrinsic tethering activity of endosomal Rab proteins. Nat Struct Mol Biol. 2011;19:40-7 pubmed publisher
    ..In our working model, the intrinsic tethering capacity Vps21 operates in concert with conventional effectors and SNAREs to drive efficient docking and fusion. ..
  5. Sun Y, Drubin D. The functions of anionic phospholipids during clathrin-mediated endocytosis site initiation and vesicle formation. J Cell Sci. 2012;125:6157-65 pubmed publisher
    ..We also demonstrated that, in various deletion mutants of genes encoding components of the Rcy1-Ypt31/32 GTPase pathway, endocytic proteins dynamically assemble not only on the plasma membrane but also on ..
  6. Yang X, Matern H, Gallwitz D. Specific binding to a novel and essential Golgi membrane protein (Yip1p) functionally links the transport GTPases Ypt1p and Ypt31p. EMBO J. 1998;17:4954-63 pubmed
    ..The results suggests for a role for Yip1p in recruiting the two GTPases to Golgi target membranes in preparation for fusion. ..
  7. Santiago Tirado F, Legesse Miller A, Schott D, Bretscher A. PI4P and Rab inputs collaborate in myosin-V-dependent transport of secretory compartments in yeast. Dev Cell. 2011;20:47-59 pubmed publisher
    ..Further, the trans-Golgi network Rab Ypt31/32p and secretory vesicle Rab Sec4p each bind directly, but distinctly, to Myo2p, and these interactions are also ..
  8. McDonold C, Fromme J. Four GTPases differentially regulate the Sec7 Arf-GEF to direct traffic at the trans-golgi network. Dev Cell. 2014;30:759-67 pubmed publisher
    ..We demonstrate that Sec7 is also an effector of two Rab GTPases, Ypt1 (Rab1) and Ypt31/32 (Rab11), signifying unprecedented signaling crosstalk between GTPase pathways...
  9. Thomas L, Fromme J. GTPase cross talk regulates TRAPPII activation of Rab11 homologues during vesicle biogenesis. J Cell Biol. 2016;215:499-513 pubmed
    ..reactions, we now provide definitive evidence that TRAPPII is a bona fide GEF for the yeast Rab11 homologues Ypt31/32. We also uncover a direct role for Arf1, a distinct GTPase, in recruiting TRAPPII to anionic membranes...
  10. Matern H, Yang X, Andrulis E, Sternglanz R, Trepte H, Gallwitz D. A novel Golgi membrane protein is part of a GTPase-binding protein complex involved in vesicle targeting. EMBO J. 2000;19:4485-92 pubmed
    ..Genetic analyses suggest that Yif1p acts downstream of Yip1p. It is inferred that Ypt GTPase binding to the Yip1p-Yif1p complex is essential for and precedes vesicle docking and fusion. ..
  11. Calero M, Collins R. Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins. Biochem Biophys Res Commun. 2002;290:676-81 pubmed
    ..The interactions between Pra1p/Yip3p and Rab proteins are dependent on the presence of the Rab protein C-terminal cysteines and require C-terminal prenylation. ..
  12. Zanolari B, Rockenbauch U, Trautwein M, Clay L, Barral Y, Spang A. Transport to the plasma membrane is regulated differently early and late in the cell cycle in Saccharomyces cerevisiae. J Cell Sci. 2011;124:1055-66 pubmed publisher
    ..We propose that transport to and from the bud neck is regulated differently in small- and large-budded cells, and differs early and late in the cell cycle. ..
  13. Tu J, Song W, Carlson M. Protein phosphatase type 1 interacts with proteins required for meiosis and other cellular processes in Saccharomyces cerevisiae. Mol Cell Biol. 1996;16:4199-206 pubmed
    ..In addition, this study indicates that the two-hybrid system offers a promising approach to understanding the multiple roles and interactions of PP1 in cellular regulation. ..
  14. Zou S, Chen Y, Liu Y, Segev N, Yu S, Liu Y, et al. Trs130 participates in autophagy through GTPases Ypt31/32 in Saccharomyces cerevisiae. Traffic. 2013;14:233-46 pubmed publisher
    ..protein particle II complex, which functions as a guanine nucleotide exchange factor (GEF) for Rab GTPases Ypt31/32. Ypt31/32 is known to be involved in autophagy, although the precise mechanism has not been thoroughly studied...
  15. Jones S, Newman C, Liu F, Segev N. The TRAPP complex is a nucleotide exchanger for Ypt1 and Ypt31/32. Mol Biol Cell. 2000;11:4403-11 pubmed
    In yeast, the Ypt1 GTPase is required for ER-to-cis-Golgi and cis-to-medial-Golgi protein transport, while Ypt31/32 are a functional pair of GTPases essential for exit from the trans-Golgi...
  16. Chen C, Collins R. Insights into biological functions across species: examining the role of Rab proteins in YIP1 family function. Biochem Soc Trans. 2005;33:614-8 pubmed
    ..e. independent or dependent activities of Yip1p. ..
  17. Tokarev A, Taussig D, Sundaram G, Lipatova Z, Liang Y, Mulholland J, et al. TRAPP II complex assembly requires Trs33 or Trs65. Traffic. 2009;10:1831-44 pubmed publisher
    ..Moreover, because humans express two Trs33 homologues, we propose that the requirement of Trs33 for TRAPP II assembly is conserved from yeast to humans. ..
  18. Lipatova Z, Tokarev A, Jin Y, Mulholland J, Weisman L, Segev N. Direct interaction between a myosin V motor and the Rab GTPases Ypt31/32 is required for polarized secretion. Mol Biol Cell. 2008;19:4177-87 pubmed publisher
    ..However, no Ypt/Rab GTPase has been shown to regulate the motility of exocytic compartments. In yeast, the Ypt31/32 functional pair is required for the formation of trans-Golgi vesicles...
  19. Ignatev A, Kravchenko S, Rak A, Goody R, Pylypenko O. A structural model of the GDP dissociation inhibitor rab membrane extraction mechanism. J Biol Chem. 2008;283:18377-84 pubmed publisher
    ..We suggest a model for the interaction of GDI with prenylated Rab proteins that incorporates a stepwise increase in affinity as the three different partial interactions are successively formed. ..
  20. Zhang C, Bowzard J, Greene M, Anido A, Stearns K, Kahn R. Genetic interactions link ARF1, YPT31/32 and TRS130. Yeast. 2002;19:1075-86 pubmed
    ..e. processing of carboxypeptidase Y or invertase. YPT31 and YPT32 were identified in a subsequent screen as high-copy suppressors of arf1(-)trs130-101...
  21. Will E, Albert S, Gallwitz D. Expression, purification, and biochemical properties of Ypt/Rab GTPase-activating proteins of Gyp family. Methods Enzymol. 2001;329:50-8 pubmed
  22. Ignatev A, Piatkov K, Pylypenko O, Rak A. A size filtration approach to purify low affinity complexes for crystallization. J Struct Biol. 2007;159:154-7 pubmed
    ..The resulting model of the low affinity unprenylated Rab-GDI complex should reflect a transient Rab-GDI complex when GDI is bound to the membrane-anchored Rab protein and is poised to extract Rab to cytosol. ..
  23. Zou S, Liu Y, Zhang X, Chen Y, Ye M, Zhu X, et al. Modular TRAPP complexes regulate intracellular protein trafficking through multiple Ypt/Rab GTPases in Saccharomyces cerevisiae. Genetics. 2012;191:451-60 pubmed publisher
    ..In yeast, Ypt1 is essential for endoplasmic reticulum (ER)-to-Golgi transport, whereas Ypt31/32 regulate Golgi-to-plasma membrane and endosome-to-Golgi transport...
  24. Morozova N, Liang Y, Tokarev A, Chen S, Cox R, Andrejic J, et al. TRAPPII subunits are required for the specificity switch of a Ypt-Rab GEF. Nat Cell Biol. 2006;8:1263-9 pubmed
    ..The conserved modular complex TRAPP is a GEF for the Golgi gatekeepers Ypt1 and Ypt31/32 (Refs 5-7). However, it is not known how Golgi entry and exit are coordinated...
  25. Kim J, Lipatova Z, Majumdar U, Segev N. Regulation of Golgi Cisternal Progression by Ypt/Rab GTPases. Dev Cell. 2016;36:440-52 pubmed publisher
    ..Moreover, controversy about the placement of two of the founding members of the Ypt/Rab family, Ypt1 and Ypt31, to specific Golgi cisternae interferes with addressing this question in yeast, where cisternal progression has ..
  26. Gorynia S, Lorenz T, Costaguta G, Daboussi L, Cascio D, Payne G. Yeast Irc6p is a novel type of conserved clathrin coat accessory factor related to small G proteins. Mol Biol Cell. 2012;23:4416-29 pubmed publisher
    ..Together these results define Irc6p/p34 as a novel type of conserved clathrin accessory protein and founding members of a new G protein-like family. ..
  27. Tsujimoto Y, Takase D, Okano H, Tomari N, Watanabe K, Matsui H. Functional roles of YPT31 and YPT32 in clotrimazole resistance of Saccharomyces cerevisiae through effects on vacuoles and ATP-binding cassette transporter(s). J Biosci Bioeng. 2013;115:4-11 pubmed publisher
    We identified YPT31, which is involved in Golgi traffic, as a clotrimazole (CTZ)-resistance gene in a multicopy library screen...
  28. Calero M, Chen C, Zhu W, Winand N, Havas K, Gilbert P, et al. Dual prenylation is required for Rab protein localization and function. Mol Biol Cell. 2003;14:1852-67 pubmed
  29. Albert S, Gallwitz D. Two new members of a family of Ypt/Rab GTPase activating proteins. Promiscuity of substrate recognition. J Biol Chem. 1999;274:33186-9 pubmed
    ..Mdr1/Gyp2p is an efficient GAP for Ypt6p and Sec4p, whereas Msb3/Gyp3p is a potent GAP for Sec4p, Ypt6p, Ypt51p, Ypt31/Ypt32p, and Ypt1p...
  30. Stalder D, Novick P. The casein kinases Yck1p and Yck2p act in the secretory pathway, in part, by regulating the Rab exchange factor Sec2p. Mol Biol Cell. 2016;27:686-701 pubmed publisher
    ..This promotes Sec2p function by stimulating its interaction with Sec15p. Finally, Sec2p is dephosphorylated very late in the exocytic reaction to facilitate recycling. ..
  31. Siniossoglou S. Affinity purification of Ypt6 effectors and identification of TMF/ARA160 as a Rab6 interactor. Methods Enzymol. 2005;403:599-607 pubmed
    ..The mammalian homolog of Sgm1, TMF/ARA160, contains a short conserved coiled-coil motif that is sufficient for the binding to the three mammalian orthologs of Ypt6, Rab6A, Rab6A', and Rab6B. ..