YPT1

Summary

Gene Symbol: YPT1
Description: Rab family GTPase YPT1
Alias: Rab family GTPase YPT1
Species: Saccharomyces cerevisiae S288c
Products:     YPT1

Top Publications

  1. Jiang Y, Scarpa A, Zhang L, Stone S, Feliciano E, Ferro Novick S. A high copy suppressor screen reveals genetic interactions between BET3 and a new gene. Evidence for a novel complex in ER-to-Golgi transport. Genetics. 1998;149:833-41 pubmed
    ..The identification of mammalian homologues of Bet3p and Bet5p implies that the Bet3p/Bet5p complex is highly conserved in evolution. ..
  2. Li B, Warner J. Genetic interaction between YPT6 and YPT1 in Saccharomyces cerevisiae. Yeast. 1998;14:915-22 pubmed
    ..Here we show that overexpression of YPT1 suppresses the growth and secretion defects of a ypt6 temperature-sensitive (ts) strain...
  3. Sclafani A, Chen S, Rivera Molina F, Reinisch K, Novick P, Ferro Novick S. Establishing a role for the GTPase Ypt1p at the late Golgi. Traffic. 2010;11:520-32 pubmed publisher
    ..Here we describe a screen for ypt1 mutants that block traffic from the early endosome to the late Golgi, but not general secretion...
  4. Du L, Collins R, Novick P. Identification of a Sec4p GTPase-activating protein (GAP) as a novel member of a Rab GAP family. J Biol Chem. 1998;273:3253-6 pubmed
    ..of Gyp1p was inhibitory in combination with a subset of secretory mutants including sec4-8 and several ypt1 mutants...
  5. Plemel R, Lobingier B, Brett C, Angers C, Nickerson D, Paulsel A, et al. Subunit organization and Rab interactions of Vps-C protein complexes that control endolysosomal membrane traffic. Mol Biol Cell. 2011;22:1353-63 pubmed publisher
    ..Biochemical and genetic experiments demonstrate the importance of these regions, revealing the Vps11 CTD as a key integrator of Vps-C complex assembly, Rab signaling, and endosomal and lysosomal traffic. ..
  6. Sapperstein S, Lupashin V, Schmitt H, Waters M. Assembly of the ER to Golgi SNARE complex requires Uso1p. J Cell Biol. 1996;132:755-67 pubmed
    ..of Ypt1p allows a delta uso1 strain to grow, whereas overexpression of Uso1p does not suppress a delta ypt1 strain...
  7. Bacon R, Salminen A, Ruohola H, Novick P, Ferro Novick S. The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants. J Cell Biol. 1989;109:1015-22 pubmed
    The YPT1 gene encodes a raslike, GTP-binding protein that is essential for growth of yeast cells...
  8. Jedd G, Richardson C, Litt R, Segev N. The Ypt1 GTPase is essential for the first two steps of the yeast secretory pathway. J Cell Biol. 1995;131:583-90 pubmed
    ..and H. Stenmark. 1993. Curr. Opin. Cell Biol. 5:613-620). However, in this report we show that the Ypt1-GTPase, a member of the rab family, is essential for more than one step of the yeast secretory pathway...
  9. Matern H, Yang X, Andrulis E, Sternglanz R, Trepte H, Gallwitz D. A novel Golgi membrane protein is part of a GTPase-binding protein complex involved in vesicle targeting. EMBO J. 2000;19:4485-92 pubmed
    ..Genetic analyses suggest that Yif1p acts downstream of Yip1p. It is inferred that Ypt GTPase binding to the Yip1p-Yif1p complex is essential for and precedes vesicle docking and fusion. ..

More Information

Publications75

  1. Lynch Day M, Bhandari D, Menon S, Huang J, Cai H, Bartholomew C, et al. Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy. Proc Natl Acad Sci U S A. 2010;107:7811-6 pubmed publisher
    ..The TRAPP complexes are multimeric guanine nucleotide exchange factors (GEFs) that activate the Rab GTPase Ypt1, which is required for secretion...
  2. Ballew N, Liu Y, Barlowe C. A Rab requirement is not bypassed in SLY1-20 suppression. Mol Biol Cell. 2005;16:1839-49 pubmed
    ..Loss-of-function ypt1 and uso1 mutations are suppressed by SLY1-20, a dominant allele that encodes the Sed5p-associated protein, Sly1p...
  3. Calero M, Chen C, Zhu W, Winand N, Havas K, Gilbert P, et al. Dual prenylation is required for Rab protein localization and function. Mol Biol Cell. 2003;14:1852-67 pubmed
    ..Substitution of different prenyl anchors on Rab GTPases does not lead to correct function. In the case of YPT1 and SEC4, two essential Rab genes in Saccharomyces cerevisiae, alternative lipid tails cannot support life when ..
  4. Barrowman J, Wang W, Zhang Y, Ferro Novick S. The Yip1p.Yif1p complex is required for the fusion competence of endoplasmic reticulum-derived vesicles. J Biol Chem. 2003;278:19878-84 pubmed
    ..Consistent with this proposal, we observe that the Yip1p.Yif1p complex binds to the ER to Golgi SNAREs Bos1p and Sec22p, two components of the membrane fusion machinery. ..
  5. Morozova N, Liang Y, Tokarev A, Chen S, Cox R, Andrejic J, et al. TRAPPII subunits are required for the specificity switch of a Ypt-Rab GEF. Nat Cell Biol. 2006;8:1263-9 pubmed
    ..The conserved modular complex TRAPP is a GEF for the Golgi gatekeepers Ypt1 and Ypt31/32 (Refs 5-7). However, it is not known how Golgi entry and exit are coordinated...
  6. Wang W, Sacher M, Ferro Novick S. TRAPP stimulates guanine nucleotide exchange on Ypt1p. J Cell Biol. 2000;151:289-96 pubmed
    ..Previous studies have shown that YPT1, which encodes the small GTP-binding protein that regulates membrane traffic at this stage of the secretory pathway,..
  7. Richardson C, Jones S, Litt R, Segev N. GTP hydrolysis is not important for Ypt1 GTPase function in vesicular transport. Mol Cell Biol. 1998;18:827-38 pubmed
    ..We demonstrate that the mutant Ypt1-Q67L protein is severely impaired in its ability to hydrolyze GTP both in the absence and in the presence of GAP ..
  8. Calero M, Winand N, Collins R. Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors. FEBS Lett. 2002;515:89-98 pubmed
  9. Jones S, Newman C, Liu F, Segev N. The TRAPP complex is a nucleotide exchanger for Ypt1 and Ypt31/32. Mol Biol Cell. 2000;11:4403-11 pubmed
    In yeast, the Ypt1 GTPase is required for ER-to-cis-Golgi and cis-to-medial-Golgi protein transport, while Ypt31/32 are a functional pair of GTPases essential for exit from the trans-Golgi...
  10. Ossig R, Dascher C, Trepte H, Schmitt H, Gallwitz D. The yeast SLY gene products, suppressors of defects in the essential GTP-binding Ypt1 protein, may act in endoplasmic reticulum-to-Golgi transport. Mol Cell Biol. 1991;11:2980-93 pubmed
    ..Here we report that four newly discovered suppressors of YPT1 deletion (SLY1-20, SLY2, SLY12, and SLY41) to a varying degree restore ER-to-Golgi transport defects in cells ..
  11. VanRheenen S, Cao X, Lupashin V, Barlowe C, Waters M. Sec35p, a novel peripheral membrane protein, is required for ER to Golgi vesicle docking. J Cell Biol. 1998;141:1107-19 pubmed
    ..The temperature-sensitive phenotype of sec35-1 is efficiently suppressed by YPT1, which encodes the rab-like GTPase required early in the secretory pathway, or by SLY1-20, which encodes a dominant ..
  12. Luan P, Balch W, Emr S, Burd C. Molecular dissection of guanine nucleotide dissociation inhibitor function in vivo. Rab-independent binding to membranes and role of Rab recycling factors. J Biol Chem. 1999;274:14806-17 pubmed
  13. Jiang Y, Ferro Novick S. Identification of yeast component A: reconstitution of the geranylgeranyltransferase that modifies Ypt1p and Sec4p. Proc Natl Acad Sci U S A. 1994;91:4377-81 pubmed
    ..Thus, these findings suggest that there is one essential escort protein in yeast, while more than one may exist in mammalian cells...
  14. Cao X, Barlowe C. Asymmetric requirements for a Rab GTPase and SNARE proteins in fusion of COPII vesicles with acceptor membranes. J Cell Biol. 2000;149:55-66 pubmed
    ..in which membranes protein function is required, temperature-sensitive alleles of BOS1, BET1, SED5, SLY1, and YPT1 that prevent ER/Golgi transport in vitro at restrictive temperatures were used to selectively inactivate these gene ..
  15. Cao X, Ballew N, Barlowe C. Initial docking of ER-derived vesicles requires Uso1p and Ypt1p but is independent of SNARE proteins. EMBO J. 1998;17:2156-65 pubmed
    ..We propose that an initial vesicle docking event of ER-derived vesicles, termed tethering, depends on Uso1p and Ypt1p but is independent of SNARE proteins...
  16. Dascher C, Ossig R, Gallwitz D, Schmitt H. Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily. Mol Cell Biol. 1991;11:872-85 pubmed
    In Saccharomyces cerevisiae, the GTP-binding Ypt1 protein (Ypt1p) is essential for endoplasmic reticulum-to-Golgi protein transport...
  17. Rossi G, Kolstad K, Stone S, Palluault F, Ferro Novick S. BET3 encodes a novel hydrophilic protein that acts in conjunction with yeast SNAREs. Mol Biol Cell. 1995;6:1769-80 pubmed
    ..These findings support the hypothesis that Bet3p may act before the assembly of the SNARE complex. ..
  18. Calero M, Collins R. Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins. Biochem Biophys Res Commun. 2002;290:676-81 pubmed
    ..The interactions between Pra1p/Yip3p and Rab proteins are dependent on the presence of the Rab protein C-terminal cysteines and require C-terminal prenylation. ..
  19. Yang X, Matern H, Gallwitz D. Specific binding to a novel and essential Golgi membrane protein (Yip1p) functionally links the transport GTPases Ypt1p and Ypt31p. EMBO J. 1998;17:4954-63 pubmed
    ..The results suggests for a role for Yip1p in recruiting the two GTPases to Golgi target membranes in preparation for fusion. ..
  20. Jones S, Jedd G, Kahn R, Franzusoff A, Bartolini F, Segev N. Genetic interactions in yeast between Ypt GTPases and Arf guanine nucleotide exchangers. Genetics. 1999;152:1543-56 pubmed
    ..The genetic interactions observed between Arf GEFs and Ypt GTPases suggest the existence of a Ypt-Arf GTPase cascade in the secretory pathway. ..
  21. Zou S, Liu Y, Zhang X, Chen Y, Ye M, Zhu X, et al. Modular TRAPP complexes regulate intracellular protein trafficking through multiple Ypt/Rab GTPases in Saccharomyces cerevisiae. Genetics. 2012;191:451-60 pubmed publisher
    Ypt/Rab are key regulators of intracellular trafficking in all eukaryotic cells. In yeast, Ypt1 is essential for endoplasmic reticulum (ER)-to-Golgi transport, whereas Ypt31/32 regulate Golgi-to-plasma membrane and endosome-to-Golgi ..
  22. Lupashin V, Waters M. t-SNARE activation through transient interaction with a rab-like guanosine triphosphatase. Science. 1997;276:1255-8 pubmed
    ..The data suggest that the Rab protein Ypt1p transiently interacts with the t-SNARE Sed5p and results in displacement of the negative regulator Sly1p, allowing subsequent formation of the v-SNARE-t-SNARE targeting complex. ..
  23. Cai Y, Chin H, Lazarova D, Menon S, Fu C, Cai H, et al. The structural basis for activation of the Rab Ypt1p by the TRAPP membrane-tethering complexes. Cell. 2008;133:1202-13 pubmed publisher
    ..This view of a multimeric membrane-tethering assembly complexed with a Rab provides a framework for understanding events preceding membrane fusion at the molecular level. ..
  24. Lipatova Z, Belogortseva N, Zhang X, Kim J, Taussig D, Segev N. Regulation of selective autophagy onset by a Ypt/Rab GTPase module. Proc Natl Acad Sci U S A. 2012;109:6981-6 pubmed publisher
    ..The yeast Ypt1 and its human functional homolog hRab1 regulate both endoplasmic reticulum (ER)-to-Golgi transport and autophagy...
  25. Kamena F, Diefenbacher M, Kilchert C, Schwarz H, Spang A. Ypt1p is essential for retrograde Golgi-ER transport and for Golgi maintenance in S. cerevisiae. J Cell Sci. 2008;121:1293-302 pubmed publisher
    ..Finally, COPI vesicles generated from Deltaypt1/SLY1-20 Golgi membranes in vitro were depleted of Emp47p and Sec22p. These data demonstrate that Ypt1p plays an essential role in retrograde transport from the Golgi to the ER. ..
  26. Antebi A, Fink G. The yeast Ca(2+)-ATPase homologue, PMR1, is required for normal Golgi function and localizes in a novel Golgi-like distribution. Mol Biol Cell. 1992;3:633-54 pubmed
    ..Some of these interactions are modulated by changes in external Ca2+ concentrations. These results imply a global role for Ca2+ in the proper function of components governing transit and processing through the secretory pathway. ..
  27. VanRheenen S, Cao X, Sapperstein S, Chiang E, Lupashin V, Barlowe C, et al. Sec34p, a protein required for vesicle tethering to the yeast Golgi apparatus, is in a complex with Sec35p. J Cell Biol. 1999;147:729-42 pubmed
    ..Finally, we describe RUD3, a novel gene identified through a genetic screen for multicopy suppressors of a mutation in USO1, which suppresses the sec34-2 mutation as well. ..
  28. Lipatova Z, Shah A, Kim J, Mulholland J, Segev N. Regulation of ER-phagy by a Ypt/Rab GTPase module. Mol Biol Cell. 2013;24:3133-44 pubmed publisher
    ..We recently showed that a Ypt module, consisting of Ypt1 and autophagy-specific upstream activator and downstream effector, regulates the onset of selective autophagy in ..
  29. Mukherjee I, Barlowe C. Overexpression of Sly41 suppresses COPII vesicle-tethering deficiencies by elevating intracellular calcium levels. Mol Biol Cell. 2016;27:1635-49 pubmed publisher
    SLY41 was identified as a multicopy suppressor of loss of Ypt1, a Rab GTPase essential for COPII vesicle tethering at the Golgi complex...
  30. Ragnini A, Teply R, Waldherr M, Voskova A, Schweyen R. The yeast protein Mrs6p, a homologue of the rabGDI and human choroideraemia proteins, affects cytoplasmic and mitochondrial functions. Curr Genet. 1994;26:308-14 pubmed
    ..with G proteins is indicated by our observation that the MRS6 gene suppresses the effect of a temperature-sensitive ypt1 mutation. Disruption of the MRS6 gene is lethal to haploid yeast cells...
  31. Du L, Novick P. Yeast rab GTPase-activating protein Gyp1p localizes to the Golgi apparatus and is a negative regulator of Ypt1p. Mol Biol Cell. 2001;12:1215-26 pubmed
    ..Conversely, a partial loss-of-function allele of YPT1, ypt1-2, can suppress the growth defect of gyp1Delta cells...
  32. Li Y, Schmitt H, Gallwitz D, Peng R. Mutations of the SM protein Sly1 resulting in bypass of GTPase requirement in vesicular transport are confined to a short helical region. FEBS Lett. 2007;581:5698-702 pubmed
    ..Here, we describe new mutants of the yeast SM protein Sly1 that specifically bypass the need for GTPases Ypt1 and Ypt6 in vesicular transport...
  33. Alory C, Balch W. Molecular basis for Rab prenylation. J Cell Biol. 2000;150:89-103 pubmed
  34. Wagner P, Molenaar C, Rauh A, Brökel R, Schmitt H, Gallwitz D. Biochemical properties of the ras-related YPT protein in yeast: a mutational analysis. EMBO J. 1987;6:2373-9 pubmed
    Using site-directed mutagenesis, the ras-related and essential yeast YPT1 gene was changed to generate proteins with amino acid exchanges within conserved regions...
  35. Kim J, Lipatova Z, Majumdar U, Segev N. Regulation of Golgi Cisternal Progression by Ypt/Rab GTPases. Dev Cell. 2016;36:440-52 pubmed publisher
    ..Moreover, controversy about the placement of two of the founding members of the Ypt/Rab family, Ypt1 and Ypt31, to specific Golgi cisternae interferes with addressing this question in yeast, where cisternal ..
  36. Lafourcade C, Galan J, Gloor Y, Haguenauer Tsapis R, Peter M. The GTPase-activating enzyme Gyp1p is required for recycling of internalized membrane material by inactivation of the Rab/Ypt GTPase Ypt1p. Mol Cell Biol. 2004;24:3815-26 pubmed
    ..Interestingly, the deletions of GYP1 and YPT6 were synthetic lethal, raising the possibility that at least two distinct pathways are involved in recycling of membrane material. ..
  37. Stearns T, Willingham M, Botstein D, Kahn R. ADP-ribosylation factor is functionally and physically associated with the Golgi complex. Proc Natl Acad Sci U S A. 1990;87:1238-42 pubmed
    ..revealed interactions between ARF1 and other genes known to be involved in the secretory pathway, including YPT1, which encodes a different GTP-binding protein...
  38. Thomas J, Zhang Y, Wei Y, Cho J, Morris L, Wang H, et al. Rab1A is an mTORC1 activator and a colorectal oncogene. Cancer Cell. 2014;26:754-69 pubmed publisher
    ..Our results demonstrate that Rab1 is an mTORC1 activator and an oncogene and that hyperactive AA signaling through Rab1A overexpression drives oncogenesis and renders cancer cells prone to mTORC1-targeted therapy. ..
  39. Collins R, Brennwald P, Garrett M, Lauring A, Novick P. Interactions of nucleotide release factor Dss4p with Sec4p in the post-Golgi secretory pathway of yeast. J Biol Chem. 1997;272:18281-9 pubmed
    ..We examined the relationship of GDI1 and DSS4 with SEC4 both genetically and biochemically. These results exclude a role for DSS4 in the recruitment of Sec4p/GDI onto membranes. ..
  40. Walch Solimena C, Novick P. The yeast phosphatidylinositol-4-OH kinase pik1 regulates secretion at the Golgi. Nat Cell Biol. 1999;1:523-5 pubmed
  41. Tsvetanova N, Riordan D, Brown P. The yeast Rab GTPase Ypt1 modulates unfolded protein response dynamics by regulating the stability of HAC1 RNA. PLoS Genet. 2012;8:e1002862 pubmed publisher
    ..We characterized the interaction of HAC1 RNA with one of these proteins, the yeast Rab1 homolog Ypt1. We found that Ypt1 protein specifically associated in vivo with unspliced HAC1 RNA...
  42. McDonold C, Fromme J. Four GTPases differentially regulate the Sec7 Arf-GEF to direct traffic at the trans-golgi network. Dev Cell. 2014;30:759-67 pubmed publisher
    ..We demonstrate that Sec7 is also an effector of two Rab GTPases, Ypt1 (Rab1) and Ypt31/32 (Rab11), signifying unprecedented signaling crosstalk between GTPase pathways...
  43. Zou S, Liu Y, Zhang C, Yu S, Liang Y. Bet3 participates in autophagy through GTPase Ypt1 in Saccharomyces cerevisiae. Cell Biol Int. 2015;39:466-74 pubmed publisher
    Three TRAPP (transport protein particle) complexes have been identified in Saccharomyces cerevisiae. GTPases Ypt1 and Ypt31/32 suppress autophagic defects in the mutants of TRAPPIII-specific subunit (Trs85) and TRAPPII-specific subunits (..
  44. Geng J, Shin M, Gilbert P, Collins R, Burd C. Saccharomyces cerevisiae Rab-GDI displacement factor ortholog Yip3p forms distinct complexes with the Ypt1 Rab GTPase and the reticulon Rtn1p. Eukaryot Cell. 2005;4:1166-74 pubmed
    ..These data indicate that Yip3p may have multiple functions and that its interaction with Rabs is not critical for their recruitment to organelle membranes. ..
  45. Cooper A, Gitler A, Cashikar A, Haynes C, Hill K, Bhullar B, et al. Alpha-synuclein blocks ER-Golgi traffic and Rab1 rescues neuron loss in Parkinson's models. Science. 2006;313:324-8 pubmed
    ..Elevated expression of Rab1, the mammalian YPT1 homolog, protected against alphaSyn-induced dopaminergic neuron loss in animal models of PD...
  46. Bauer B, Lorenzetti S, Miaczynska M, Bui D, Schweyen R, Ragnini A. Amino- and carboxy-terminal domains of the yeast Rab escort protein are both required for binding of Ypt small G proteins. Mol Biol Cell. 1996;7:1521-33 pubmed
    ..This analysis has shown that the SCRs are necessary but not sufficient for Ypt1p binding by the yeast REP, the carboxy-terminal region also being required. ..
  47. Gilbert P, Burd C. GDP dissociation inhibitor domain II required for Rab GTPase recycling. J Biol Chem. 2001;276:8014-20 pubmed
    ..We conclude that Domain II of Gdi1p is essential for Rab loading and Rab extraction, and confirm that each of these activities is required for Gdi1p function in vivo. ..
  48. Lipatova Z, Majumdar U, Segev N. Trs33-Containing TRAPP IV: A Novel Autophagy-Specific Ypt1 GEF. Genetics. 2016;204:1117-1128 pubmed publisher
    ..Here, we identify a novel GEF complex, TRAPP IV, which regulates Ypt1-mediated autophagy...
  49. Chabane S, Gachet E, Kepes F. Over-expression of the yeast BFR2 gene partially suppresses the growth defects induced by Brefeldin A and by four ER-to-Golgi mutations. Curr Genet. 1998;33:21-8 pubmed
    ..blocked at the step of budding or docking of small vesicles en route to the Golgi (sec13-1, sec16-2, sec23-1, ypt1-1)...
  50. Yoo J, Grabowski R, Xing L, Trepte H, Schmitt H, Gallwitz D. Functional implications of genetic interactions between genes encoding small GTPases involved in vesicular transport in yeast. Mol Gen Genet. 1999;261:80-91 pubmed
    ..YPT32, whose gene products have a reduced affinity for GTP, are able to suppress the dominant lethal phenotype of YPT1(N121I)...
  51. Behnia R, Barr F, Flanagan J, Barlowe C, Munro S. The yeast orthologue of GRASP65 forms a complex with a coiled-coil protein that contributes to ER to Golgi traffic. J Cell Biol. 2007;176:255-61 pubmed
    ..for growth, but biochemical assays and genetic interactions with known mediators of vesicle tethering (Uso1 and Ypt1) suggest that the Grh1-Bug1 complex contributes to a redundant network of interactions that mediates consumption of ..
  52. De Antoni A, Schmitzová J, Trepte H, Gallwitz D, Albert S. Significance of GTP hydrolysis in Ypt1p-regulated endoplasmic reticulum to Golgi transport revealed by the analysis of two novel Ypt1-GAPs. J Biol Chem. 2002;277:41023-31 pubmed
    ..2 x 10(4)-fold and, surprisingly, the 40-fold reduced GTP hydrolysis rate of Ypt1(Q67L)p 1.5 x 10(4)-fold...
  53. Lo S, Brett C, Plemel R, Vignali M, Fields S, Gonen T, et al. Intrinsic tethering activity of endosomal Rab proteins. Nat Struct Mol Biol. 2011;19:40-7 pubmed publisher
    ..In our working model, the intrinsic tethering capacity Vps21 operates in concert with conventional effectors and SNAREs to drive efficient docking and fusion. ..
  54. Rak A, Pylypenko O, Durek T, Watzke A, Kushnir S, Brunsveld L, et al. Structure of Rab GDP-dissociation inhibitor in complex with prenylated YPT1 GTPase. Science. 2003;302:646-50 pubmed
    ..Using a combination of chemical synthesis and protein engineering, we generated and crystallized the monoprenylated Ypt1:RabGDI complex. The structure of the complex was solved to 1...
  55. Kang C, Lee S, Park J, Lee Y, Jung H, Chi Y, et al. Stress-driven structural and functional switching of Ypt1p from a GTPase to a molecular chaperone mediates thermo tolerance in Saccharomyces cerevisiae. FASEB J. 2015;29:4424-34 pubmed publisher
    ..Subsequently, we found, by using a galactose-inducible expression system, that conditional overexpression of YPT1 in yeast cells enhanced the thermotolerance of cells by increasing the survival rate at 55°C by ∼60%, ..
  56. Rivera Molina F, Novick P. A Rab GAP cascade defines the boundary between two Rab GTPases on the secretory pathway. Proc Natl Acad Sci U S A. 2009;106:14408-13 pubmed publisher
    ..GEF cascades working in concert with counter-current GAP cascades could generate a programmed series of Rab conversions responsible for regulating the choreography of membrane traffic. ..
  57. Prosser D, Pannunzio A, Brodsky J, Thorner J, Wendland B, O Donnell A. α-Arrestins participate in cargo selection for both clathrin-independent and clathrin-mediated endocytosis. J Cell Sci. 2015;128:4220-34 pubmed publisher
    ..Notably, neither expression of Rsp5 nor its binding to α-arrestins is required for CIE. Thus, α-arrestins are important for cargo selection in both the CME and CIE pathways, but function by distinct mechanisms in each pathway. ..
  58. Chin H, Cai Y, Menon S, Ferro Novick S, Reinisch K, De La Cruz E. Kinetic analysis of the guanine nucleotide exchange activity of TRAPP, a multimeric Ypt1p exchange factor. J Mol Biol. 2009;389:275-88 pubmed publisher
    ..These results illustrate a novel strategy of guanine nucleotide exchange by TRAPP that is particularly suited for a multifunctional GEF involved in membrane traffic. ..
  59. Suvorova E, Duden R, Lupashin V. The Sec34/Sec35p complex, a Ypt1p effector required for retrograde intra-Golgi trafficking, interacts with Golgi SNAREs and COPI vesicle coat proteins. J Cell Biol. 2002;157:631-43 pubmed
    ..We propose that the Sec34/35 protein complex acts as a tether that connects cis-Golgi membranes and COPI-coated, retrogradely targeted intra-Golgi vesicles. ..
  60. Parrish W, Stefan C, Emr S. PtdIns(3)P accumulation in triple lipid-phosphatase-deletion mutants triggers lethal hyperactivation of the Rho1p/Pkc1p cell-integrity MAP kinase pathway. J Cell Sci. 2005;118:5589-601 pubmed
    ..Taken together, the results of this study indicated that aberrant Rho1p/Pkc1p signaling contributes to the lethal effects of PtdIns(3)P accumulation in cells deficient in PI 3-phosphatase activity. ..
  61. Tran L, Bang S, Yoon J, Kim Y, Min J. Effect of GTP-binding protein (YPT1 protein) on the enhanced yeast vacuolar activity. Mol Cell Biochem. 2016;414:179-86 pubmed publisher
    Yeast GTP-binding protein (YPT1 protein) has been reported to function in the early stages of the secretory pathway. Particularly, YPT1 protein is observed to regulate both the endoplasmic reticulum-to-Golgi transport and the autophagy...
  62. Cho J, Noda Y, Yoda K. Proteins in the early golgi compartment of Saccharomyces cerevisiae immunoisolated by Sed5p. FEBS Lett. 2000;469:151-4 pubmed
  63. Finger F, Novick P. Sec3p is involved in secretion and morphogenesis in Saccharomyces cerevisiae. Mol Biol Cell. 1997;8:647-62 pubmed
    ..The SEC3 gene in high copy suppresses pfy1-111 and sec5-24 and causes synthetic growth defects with ypt1, sec8-9, sec10-2, and sec15-1...
  64. Singh J, Tyers M. A Rab escort protein integrates the secretion system with TOR signaling and ribosome biogenesis. Genes Dev. 2009;23:1944-58 pubmed publisher
    ..The Sfp1-Mrs6 interaction is a nexus for growth regulation that links the secretory system and TOR-dependent nutrient signaling to ribosome biogenesis. ..
  65. Dursina B, Thoma N, Sidorovitch V, Niculae A, Iakovenko A, Rak A, et al. Interaction of yeast Rab geranylgeranyl transferase with its protein and lipid substrates. Biochemistry. 2002;41:6805-16 pubmed
    ..Despite the ability to function in concert with yRabGGTase in vitro, expression of mammalian REP-1 could not complement deletion of MRS6 gene in S. cerevisiae in vivo. The implications of these findings are discussed. ..
  66. Lipatova Z, Segev N. A Role for Macro-ER-Phagy in ER Quality Control. PLoS Genet. 2015;11:e1005390 pubmed publisher
    ..three sequential steps of this pathway are delineated: Atg9-dependent exit from the ER en route to autophagy, Ypt1- and core Atgs-mediated pre-autophagsomal-structure organization, and Ypt51-mediated delivery of APs to the vacuole...