Gene Symbol: YPS1
Description: aspartyl protease
Alias: YAP3, aspartyl protease
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Gagnon Arsenault I, Parisé L, Tremblay J, Bourbonnais Y. Activation mechanism, functional role and shedding of glycosylphosphatidylinositol-anchored Yps1p at the Saccharomyces cerevisiae cell surface. Mol Microbiol. 2008;69:982-93 pubmed publisher
    ..Collectively, our data provide a direct link between the pH regulation of yeast cell wall assembly and the activity of a yapsin. ..
  2. Miller K, Didone L, Krysan D. Extracellular secretion of overexpressed glycosylphosphatidylinositol-linked cell wall protein Utr2/Crh2p as a novel protein quality control mechanism in Saccharomyces cerevisiae. Eukaryot Cell. 2010;9:1669-79 pubmed publisher
    ..cerevisiae. This model provides an explanation for the deleterious effects of Yps1/2p on the industrial production of some recombinant proteins in S. cerevisiae.
  3. Krysan D, Ting E, Abeijon C, Kroos L, Fuller R. Yapsins are a family of aspartyl proteases required for cell wall integrity in Saccharomyces cerevisiae. Eukaryot Cell. 2005;4:1364-74 pubmed
    ..b>YPS1 expression is induced during cell wall stress and remodeling in a PKC1-MPK1-dependent manner, indicating that Yps1p ..
  4. Egel Mitani M, Flygenring H, Hansen M. A novel aspartyl protease allowing KEX2-independent MF alpha propheromone processing in yeast. Yeast. 1990;6:127-37 pubmed
    ..cerevisiae. In contrast to the BAR1 gene product, the novel aspartyl protease (YAP3 for Yeast Aspartyl Protease 3) contains a C-terminal serine/threonine-rich sequence and potential ..
  5. Tomishige N, Noda Y, Adachi H, Shimoi H, Takatsuki A, Yoda K. Mutations that are synthetically lethal with a gas1Delta allele cause defects in the cell wall of Saccharomyces cerevisiae. Mol Genet Genomics. 2003;269:562-73 pubmed
    ..A cell wall defect was also found in the kex2Delta mutant, which was suppressible by multiple copies of the MKC7 or YAP3 gene, both of which encode other GPI-anchored proteases...
  6. Komano H, Fuller R. Shared functions in vivo of a glycosyl-phosphatidylinositol-linked aspartyl protease, Mkc7, and the proprotein processing protease Kex2 in yeast. Proc Natl Acad Sci U S A. 1995;92:10752-6 pubmed
    ..MKC7 encodes an aspartyl protease most closely related to product of the YAP3 gene, a previously isolated multicopy suppressor of the pro-..
  7. Castillon G, Aguilera Romero A, Manzano López J, Epstein S, Kajiwara K, Funato K, et al. The yeast p24 complex regulates GPI-anchored protein transport and quality control by monitoring anchor remodeling. Mol Biol Cell. 2011;22:2924-36 pubmed publisher
    ..Therefore the p24 complex, by sensing the status of the GPI anchor, regulates GPI-anchored protein intracellular transport and coordinates this with correct anchor remodeling. ..
  8. Oluwatosin Y, Kane P. Mutations in the yeast KEX2 gene cause a Vma(-)-like phenotype: a possible role for the Kex2 endoprotease in vacuolar acidification. Mol Cell Biol. 1998;18:1534-43 pubmed
    ..This is the first time a mutation of this type has been identified. ..
  9. Azaryan A, Wong M, Friedman T, Cawley N, Estivariz F, Chen H, et al. Purification and characterization of a paired basic residue-specific yeast aspartic protease encoded by the YAP3 gene. Similarity to the mammalian pro-opiomelanocortin-converting enzyme. J Biol Chem. 1993;268:11968-75 pubmed
    Yeast cells express an alternate enzyme encoded by the YAP3 gene which can process pro-alpha-mating factor when this pheromone is overexpressed in KEX2-deficient mutants...

More Information


  1. Dubé A, Bélanger M, Gagnon Arsenault I, Bourbonnais Y. N-terminal entrance loop of yeast Yps1 and O-glycosylation of substrates are determinant factors controlling the shedding activity of this GPI-anchored endopeptidase. BMC Microbiol. 2015;15:50 pubmed publisher
    S. cerevisiae Yps1 is the prototypical aspartic endopeptidase of the fungal yapsin family. This glycosylphosphatidylinositol (GPI) anchored enzyme was recently shown to be involved in the shedding of the GPI proteins Utr2, Gas1 and itself...
  2. Hamada K, Terashima H, Arisawa M, Yabuki N, Kitada K. Amino acid residues in the omega-minus region participate in cellular localization of yeast glycosylphosphatidylinositol-attached proteins. J Bacteriol. 1999;181:3886-9 pubmed
    ..These correlations clearly suggest that GPI-attached proteins have two different signals which act positively or negatively in cell wall incorporation for their cellular localization. ..
  3. Cawley N, Chen H, Beinfeld M, Loh Y. Specificity and kinetic studies on the cleavage of various prohormone mono- and paired-basic residue sites by yeast aspartic protease 3. J Biol Chem. 1996;271:4168-76 pubmed
    ..1 x 10(6) m-1 s-1), while that of the monobasic site of CCK13-33 and the paired-basic site of proinsulin B-C junction, were cleaved less efficiently at 4.2 x 10(4) m-1 s-1 and 1.6 x 10(4) m-1 s-1, respectively. ..
  4. Cho E, Cheon S, Kim H, Choo J, Lee D, Ryu H, et al. Multiple-yapsin-deficient mutant strains for high-level production of intact recombinant proteins in Saccharomyces cerevisiae. J Biotechnol. 2010;149:1-7 pubmed publisher
    ..The present data strongly indicate the involvement of other yapsin members besides Yps1p in the proteolysis of secretory recombinant proteins, particularly under high-density growth conditions. ..
  5. Zhang W, Espinoza D, Hines V, Innis M, Mehta P, Miller D. Characterization of beta-amyloid peptide precursor processing by the yeast Yap3 and Mkc7 proteases. Biochim Biophys Acta. 1997;1359:110-22 pubmed
    ..Deletions of the genes encoding the GPI-linked aspartyl proteases Yap3 and Mkc7 decreased alpha-secretase activity by 56 and 29%, respectively; whereas, the double deletion decreased the ..
  6. Vadaie N, Dionne H, Akajagbor D, Nickerson S, Krysan D, Cullen P. Cleavage of the signaling mucin Msb2 by the aspartyl protease Yps1 is required for MAPK activation in yeast. J Cell Biol. 2008;181:1073-81 pubmed publisher
    ..Cleavage of the extracellular inhibitory domain of Msb2p by the aspartyl protease Yps1p generated the active form of the protein by a mechanism incorporating cellular nutritional status...
  7. Ash J, Dominguez M, Bergeron J, Thomas D, Bourbonnais Y. The yeast proprotein convertase encoded by YAP3 is a glycophosphatidylinositol-anchored protein that localizes to the plasma membrane. J Biol Chem. 1995;270:20847-54 pubmed
    The yeast YAP3 gene encodes an aspartyl endoprotease that cleaves precursor proteins at selected pairs of basic amino acids and after single arginine residues...
  8. Adhikari H, Vadaie N, Chow J, Caccamise L, Chavel C, Li B, et al. Role of the unfolded protein response in regulating the mucin-dependent filamentous-growth mitogen-activated protein kinase pathway. Mol Cell Biol. 2015;35:1414-32 pubmed publisher
    ..We speculate that the QC pathways broadly regulate signaling glycoproteins and their cognate pathways by recognizing altered glycosylation patterns that can occur in response to extrinsic cues. ..