Gene Symbol: YPK2
Description: putative protein kinase YPK2
Alias: YKR2
Species: Saccharomyces cerevisiae S288c
Products:     YPK2

Top Publications

  1. Gelperin D, Horton L, Dechant A, Hensold J, Lemmon S. Loss of ypk1 function causes rapamycin sensitivity, inhibition of translation initiation and synthetic lethality in 14-3-3-deficient yeast. Genetics. 2002;161:1453-64 pubmed
    ..These results suggest that Ypk regulates translation initiation in response to nutrient signals, either through the TOR pathway or in a functionally related pathway parallel to TOR. ..
  2. Casamayor A, Torrance P, Kobayashi T, Thorner J, Alessi D. Functional counterparts of mammalian protein kinases PDK1 and SGK in budding yeast. Curr Biol. 1999;9:186-97 pubmed
    ..In addition, the yeast YPK1 and YKR2 genes were found to encode protein kinases each with a catalytic domain closely resembling that of SGK; both Ypk1 ..
  3. Chen P, Lee K, Levin D. A pair of putative protein kinase genes (YPK1 and YPK2) is required for cell growth in Saccharomyces cerevisiae. Mol Gen Genet. 1993;236:443-7 pubmed
    ..Here we report the isolation and genetic characterization of a pair of previously described genes (YPK1 and YPK2) which are predicted to encode protein kinases that share 90% amino acid identity with each other and 44-46% ..
  4. Liu K, Zhang X, Lester R, Dickson R. The sphingoid long chain base phytosphingosine activates AGC-type protein kinases in Saccharomyces cerevisiae including Ypk1, Ypk2, and Sch9. J Biol Chem. 2005;280:22679-87 pubmed
    ..Thus, our data support a model in which PHS, rather than simply being an upstream activator of Pkh1, also activates kinases that are downstream targets of Pkh1 including Ypk1/2 and Sch9. ..
  5. Kamada Y, Fujioka Y, Suzuki N, Inagaki F, Wullschleger S, Loewith R, et al. Tor2 directly phosphorylates the AGC kinase Ypk2 to regulate actin polarization. Mol Cell Biol. 2005;25:7239-48 pubmed
    ..Screening for multicopy suppressors of tor2, we obtained a plasmid expressing an N-terminally truncated Ypk2 protein kinase...
  6. Aronova S, Wedaman K, Aronov P, Fontes K, Ramos K, Hammock B, et al. Regulation of ceramide biosynthesis by TOR complex 2. Cell Metab. 2008;7:148-58 pubmed publisher
    ..We find that TORC2 regulates this step in part by activating the AGC kinase Ypk2 and that this step is antagonized by the Ca2+/calmodulin-dependent phosphatase calcineurin...
  7. Niles B, Mogri H, Hill A, Vlahakis A, Powers T. Plasma membrane recruitment and activation of the AGC kinase Ypk1 is mediated by target of rapamycin complex 2 (TORC2) and its effector proteins Slm1 and Slm2. Proc Natl Acad Sci U S A. 2012;109:1536-41 pubmed publisher
    The yeast AGC kinase orthologs Ypk1 and Ypk2 control several important cellular processes, including actin polarization, endocytosis, and sphingolipid metabolism...
  8. Nomura W, Inoue Y. Methylglyoxal activates the target of rapamycin complex 2-protein kinase C signaling pathway in Saccharomyces cerevisiae. Mol Cell Biol. 2015;35:1269-80 pubmed publisher
    ..Methylglyoxal activated mammalian TORC2 signaling, which, in turn, phosphorylated Akt at Ser(473). Our results suggest that methylglyoxal is a conserved initiator of TORC2 signaling among eukaryotes. ..
  9. Grosshans B, Grötsch H, Mukhopadhyay D, Fernández I, Pfannstiel J, Idrissi F, et al. TEDS site phosphorylation of the yeast myosins I is required for ligand-induced but not for constitutive endocytosis of the G protein-coupled receptor Ste2p. J Biol Chem. 2006;281:11104-14 pubmed

More Information


  1. Hatakeyama R, Kono K, Yoshida S. Ypk1 and Ypk2 kinases maintain Rho1 at the plasma membrane by flippase-dependent lipid remodeling after membrane stresses. J Cell Sci. 2017;130:1169-1178 pubmed publisher
    The plasma membrane (PM) is frequently challenged by mechanical stresses. In budding yeast, TORC2-Ypk1/Ypk2 kinase cascade plays a crucial role in PM stress responses by reorganizing the actin cytoskeleton via Rho1 GTPase...
  2. Gaubitz C, Oliveira T, Prouteau M, Leitner A, Karuppasamy M, Konstantinidou G, et al. Molecular Basis of the Rapamycin Insensitivity of Target Of Rapamycin Complex 2. Mol Cell. 2015;58:977-88 pubmed publisher
    ..Removal of this sequence generated a FKBP12-rapamycin-sensitive TORC2 variant, which provides a powerful tool for deciphering TORC2 function in vivo. Using this variant, we demonstrate a role for TORC2 in G2/M cell-cycle progression. ..
  3. Shimada K, Filipuzzi I, Stahl M, Helliwell S, Studer C, Hoepfner D, et al. TORC2 signaling pathway guarantees genome stability in the face of DNA strand breaks. Mol Cell. 2013;51:829-39 pubmed publisher
    ..Genetic inhibition of Tor2 kinase, or its downstream effector kinases Ypk1/Ypk2, conferred similar synergistic effects in the presence of Zeocin...
  4. Sun Y, Miao Y, Yamane Y, Zhang C, Shokat K, Takematsu H, et al. Orm protein phosphoregulation mediates transient sphingolipid biosynthesis response to heat stress via the Pkh-Ypk and Cdc55-PP2A pathways. Mol Biol Cell. 2012;23:2388-98 pubmed publisher
    ..We propose that this mechanism serves as the basis for how Orm phosphoregulation controls sphingolipid biosynthesis in response to stress in a kinetically coupled manner. ..
  5. Liao H, Chen M. Target of rapamycin complex 2 signals to downstream effector yeast protein kinase 2 (Ypk2) through adheres-voraciously-to-target-of-rapamycin-2 protein 1 (Avo1) in Saccharomyces cerevisiae. J Biol Chem. 2012;287:6089-99 pubmed publisher
    ..b>Ypk2 (yeast protein kinase 2), a member of the cAMP-dependent, cGMP-dependent, and PKC (AGC) kinase family, is a TORC2 ..
  6. Luo G, Gruhler A, Liu Y, Jensen O, Dickson R. The sphingolipid long-chain base-Pkh1/2-Ypk1/2 signaling pathway regulates eisosome assembly and turnover. J Biol Chem. 2008;283:10433-44 pubmed publisher
    ..Eisosomes then control endocytosis to align the composition and function of the plasma membrane to match demand. ..
  7. Roelants F, Baltz A, TROTT A, Fereres S, Thorner J. A protein kinase network regulates the function of aminophospholipid flippases. Proc Natl Acad Sci U S A. 2010;107:34-9 pubmed publisher
    ..Two such targets are related protein kinases Ypk1 and Ypk2 (orthologs of mammalian SGK1), which have been implicated in multiple processes, including endocytosis and coupling ..
  8. Kennedy M, Gable K, Niewola Staszkowska K, Abreu S, Johnston A, Harris L, et al. A neurotoxic glycerophosphocholine impacts PtdIns-4, 5-bisphosphate and TORC2 signaling by altering ceramide biosynthesis in yeast. PLoS Genet. 2014;10:e1004010 pubmed publisher
  9. Roelants F, Torrance P, Bezman N, Thorner J. Pkh1 and Pkh2 differentially phosphorylate and activate Ypk1 and Ykr2 and define protein kinase modules required for maintenance of cell wall integrity. Mol Biol Cell. 2002;13:3005-28 pubmed
    ..They activate two closely related, functionally redundant enzymes, Ypk1 and Ykr2 (homologs of mammalian protein kinase, serum- and glucocorticoid-inducible protein kinase)...
  10. Yerlikaya S, Meusburger M, Kumari R, Huber A, Anrather D, Costanzo M, et al. TORC1 and TORC2 work together to regulate ribosomal protein S6 phosphorylation in Saccharomyces cerevisiae. Mol Biol Cell. 2016;27:397-409 pubmed publisher
  11. Chauhan N, Han G, Somashekarappa N, Gable K, Dunn T, Kohlwein S. Regulation of Sphingolipid Biosynthesis by the Morphogenesis Checkpoint Kinase Swe1. J Biol Chem. 2016;291:2524-34 pubmed publisher
  12. Schmelzle T, Helliwell S, Hall M. Yeast protein kinases and the RHO1 exchange factor TUS1 are novel components of the cell integrity pathway in yeast. Mol Cell Biol. 2002;22:1329-39 pubmed
    ..Activation of PKC1 occurs via the GTPase RHO1 and the kinase pair PKH1 and PKH2. Here we report that YPK1 and YPK2, an essential pair of homologous kinases and proposed downstream effectors of PKH and sphingolipids, are also ..