Gene Symbol: YDJ1
Description: type I HSP40 co-chaperone YDJ1
Alias: HSP40, MAB3, MAS5, type I HSP40 co-chaperone YDJ1
Species: Saccharomyces cerevisiae S288c
Products:     YDJ1

Top Publications

  1. Shaner L, Sousa R, Morano K. Characterization of Hsp70 binding and nucleotide exchange by the yeast Hsp110 chaperone Sse1. Biochemistry. 2006;45:15075-84 pubmed
    ..activity by Sse1 is physiologically significant, as deletion of both SSE1 and the Ssa ATPase stimulatory protein YDJ1 is synthetically lethal...
  2. Liu X, Morano K, Thiele D. The yeast Hsp110 family member, Sse1, is an Hsp90 cochaperone. J Biol Chem. 1999;274:26654-60 pubmed
    ..Taken together, these data establish Sse1 as an integral new component of the Hsp90 chaperone complex in yeast. ..
  3. McClellan A, Endres J, Vogel J, Palazzi D, Rose M, Brodsky J. Specific molecular chaperone interactions and an ATP-dependent conformational change are required during posttranslational protein translocation into the yeast ER. Mol Biol Cell. 1998;9:3533-45 pubmed
    ..We conclude that a conformation- and ATP-dependent interaction of BiP with the J domain of Sec63p is essential for protein translocation and that the specificity of hsc70 action is dictated by their DnaJ partners. ..
  4. Shorter J, Lindquist S. Hsp104, Hsp70 and Hsp40 interplay regulates formation, growth and elimination of Sup35 prions. EMBO J. 2008;27:2712-24 pubmed publisher
    ..We identify the ribosome-associated Ssb1:Zuo1:Ssz1 complex as a potent antagonist of Sup35 prionogenesis. The Hsp40 chaperones, Sis1 and Ydj1, preferentially interact with Sup35 oligomers and fibres compared with monomers, and ..
  5. Glover J, Lindquist S. Hsp104, Hsp70, and Hsp40: a novel chaperone system that rescues previously aggregated proteins. Cell. 1998;94:73-82 pubmed
    ..Unlike other chaperones, Hsp104 does not prevent the aggregation of denatured proteins. However, in concert with Hsp40 and Hsp70, Hsp104 can reactivate proteins that have been denatured and allowed to aggregate, substrates refractory ..
  6. Lopez N, Aron R, Craig E. Specificity of class II Hsp40 Sis1 in maintenance of yeast prion [RNQ+]. Mol Biol Cell. 2003;14:1172-81 pubmed
    Sis1 and Ydj1, functionally distinct heat shock protein (Hsp)40 molecular chaperones of the yeast cytosol, are homologs of Hdj1 and Hdj2 of mammalian cells, respectively...
  7. Becker J, Walter W, Yan W, Craig E. Functional interaction of cytosolic hsp70 and a DnaJ-related protein, Ydj1p, in protein translocation in vivo. Mol Cell Biol. 1996;16:4378-86 pubmed
    ..proteins are known to interact with hsp70s and strains containing conditional mutations in a dnaJ-related gene, YDJ1, are defective in translocation of prepro-alpha-factor, we looked for a genetic interaction between SSA genes and ..
  8. Nakatsukasa K, Huyer G, Michaelis S, Brodsky J. Dissecting the ER-associated degradation of a misfolded polytopic membrane protein. Cell. 2008;132:101-12 pubmed publisher
    ..These data indicate that polytopic membrane proteins can be extracted from the ER, and define the point of action of chaperones and the requirement for Ufd2p during membrane protein quality control...
  9. Mandal A, Nillegoda N, Chen J, Caplan A. Ydj1 protects nascent protein kinases from degradation and controls the rate of their maturation. Mol Cell Biol. 2008;28:4434-44 pubmed publisher
    Ydj1 is a Saccharomyces cerevisiae Hsp40 molecular chaperone that functions with Hsp70 to promote polypeptide folding...

More Information


  1. Ramos C, Oliveira C, Fan C, Torriani I, Cyr D. Conserved central domains control the quaternary structure of type I and type II Hsp40 molecular chaperones. J Mol Biol. 2008;383:155-66 pubmed publisher
    ..The Hsp40 family is large, and specialized family members direct Hsp70 to perform highly specific tasks...
  2. Flom G, Lemieszek M, Fortunato E, Johnson J. Farnesylation of Ydj1 is required for in vivo interaction with Hsp90 client proteins. Mol Biol Cell. 2008;19:5249-58 pubmed publisher
    Ydj1 of Saccharomyces cerevisiae is an abundant cytosolic Hsp40, or J-type, molecular chaperone...
  3. Flom G, Weekes J, Williams J, Johnson J. Effect of mutation of the tetratricopeptide repeat and asparatate-proline 2 domains of Sti1 on Hsp90 signaling and interaction in Saccharomyces cerevisiae. Genetics. 2006;172:41-51 pubmed
    ..We further examined the genetic and physical interaction of Sti1 with a mutant form of Hsp90, providing insight into the importance of the TPR2A domain of Sti1 in regulating Hsp90 function. ..
  4. Cyr D, Lu X, Douglas M. Regulation of Hsp70 function by a eukaryotic DnaJ homolog. J Biol Chem. 1992;267:20927-31 pubmed
    ..Thus, Hsp70SSA1 and YDJ1p interact functionally in the execution of Hsp70SSA1 chaperone activities in the eukaryotic cell. ..
  5. Bagriantsev S, Gracheva E, Richmond J, Liebman S. Variant-specific [PSI+] infection is transmitted by Sup35 polymers within [PSI+] aggregates with heterogeneous protein composition. Mol Biol Cell. 2008;19:2433-43 pubmed publisher
    ..Using a candidate approach, we detected Hsp104, Ssb1/2, Sis1, Sse1, Ydj1, and Sla2 among minor components of the aggregates...
  6. Fan C, Lee S, Ren H, Cyr D. Exchangeable chaperone modules contribute to specification of type I and type II Hsp40 cellular function. Mol Biol Cell. 2004;15:761-73 pubmed
    b>Hsp40 family members regulate Hsp70s ability to bind nonnative polypeptides and thereby play an essential role in cell physiology...
  7. Goeckeler J, Stephens A, Lee P, Caplan A, Brodsky J. Overexpression of yeast Hsp110 homolog Sse1p suppresses ydj1-151 thermosensitivity and restores Hsp90-dependent activity. Mol Biol Cell. 2002;13:2760-70 pubmed
    ..action, and to identify functional partners, we screened for multicopy suppressors of the temperature-sensitive ydj1-151 mutant and identified a yeast Hsp110, SSE1...
  8. Lee P, Shabbir A, Cardozo C, Caplan A. Sti1 and Cdc37 can stabilize Hsp90 in chaperone complexes with a protein kinase. Mol Biol Cell. 2004;15:1785-92 pubmed
    ..These data suggest that Cdc37 and Sti1 have functional overlap in stabilizing Hsp90:client complexes. Finally, we show that Cns1 functions in MAP kinase signaling in association with Cpr7. ..
  9. Savistchenko J, Krzewska J, Fay N, Melki R. Molecular chaperones and the assembly of the prion Ure2p in vitro. J Biol Chem. 2008;283:15732-9 pubmed publisher
    ..Here we investigate the effects of molecular chaperones from the Hsp40, Hsp70, Hsp90, and Hsp100 families and the chaperonin CCT/Tric on the assembly of full-length Ure2p...
  10. Sahi C, Craig E. Network of general and specialty J protein chaperones of the yeast cytosol. Proc Natl Acad Sci U S A. 2007;104:7163-8 pubmed
    ..In contrast, the severe growth phenotype caused by the absence of the cytosol's most abundant J protein, Ydj1, was substantially rescued by expression of J domain-containing fragments of many cytosolic J proteins...
  11. Hernández M, Chadli A, Toft D. HSP40 binding is the first step in the HSP90 chaperoning pathway for the progesterone receptor. J Biol Chem. 2002;277:11873-81 pubmed
    ..However, an in vitro chaperoning system consisting of five proteins (HSP40, HSP70, HOP, HSP90, and p23) with ATP is capable of restoring this function...
  12. Zhang L, Hach A, Wang C. Molecular mechanism governing heme signaling in yeast: a higher-order complex mediates heme regulation of the transcriptional activator HAP1. Mol Cell Biol. 1998;18:3819-28 pubmed
    ..Our data suggest that this complex contains HAP1 and four other cellular proteins including Hsp82 and Ydj1. The formation of this complex is directly correlated with HAP1 repression in the absence of heme, and mutational ..
  13. Summers D, Douglas P, Ren H, Cyr D. The type I Hsp40 Ydj1 utilizes a farnesyl moiety and zinc finger-like region to suppress prion toxicity. J Biol Chem. 2009;284:3628-39 pubmed publisher
    ..Thus, we examined the mechanism for binding between the Type I Hsp40 Ydj1 and the yeast prion [RNQ+]...
  14. Flom G, Weekes J, Johnson J. Novel interaction of the Hsp90 chaperone machine with Ssl2, an essential DNA helicase in Saccharomyces cerevisiae. Curr Genet. 2005;47:368-80 pubmed
    ..We obtained mutations in genes encoding components of the Hsp90 chaperone machine, HSC82, CPR7 and YDJ1, and two essential genes, SSL2 and UTP21, not previously linked to Hsp90...
  15. Caplan A, Douglas M. Characterization of YDJ1: a yeast homologue of the bacterial dnaJ protein. J Cell Biol. 1991;114:609-21 pubmed
    The YDJ1 (yeast dnaJ) gene was isolated from a yeast expression library using antisera made against a yeast nuclear sub-fraction termed the matrix lamina pore complex...
  16. Mandal A, Gibney P, Nillegoda N, Theodoraki M, Caplan A, Morano K. Hsp110 chaperones control client fate determination in the hsp70-Hsp90 chaperone system. Mol Biol Cell. 2010;21:1439-48 pubmed publisher
    ..These findings support a model in which Hsp110 chaperones contribute significantly to the decision made by Hsp70 to fold or degrade a client protein. ..
  17. Jones G, Song Y, Chung S, Masison D. Propagation of Saccharomyces cerevisiae [PSI+] prion is impaired by factors that regulate Hsp70 substrate binding. Mol Cell Biol. 2004;24:3928-37 pubmed
    ..We also find that the GPTVEEVD motif is important for physical interaction with Hsp40 (Ydj1p), another Hsp70 cochaperone that promotes substrate binding but is dispensable for viability...
  18. Johnson J, Craig E. An essential role for the substrate-binding region of Hsp40s in Saccharomyces cerevisiae. J Cell Biol. 2001;152:851-6 pubmed
    ..As an in vivo test of this model, requirements for viability were examined in a strain having deletions of both Hsp40 genes...
  19. Caplan A, Cyr D, Douglas M. YDJ1p facilitates polypeptide translocation across different intracellular membranes by a conserved mechanism. Cell. 1992;71:1143-55 pubmed
    The role of S. cerevisiae YDJ1 protein (YDJ1p) in polypeptide translocation across membranes has been examined...
  20. Lian H, Zhang H, Zhang Z, Loovers H, Jones G, Rowling P, et al. Hsp40 interacts directly with the native state of the yeast prion protein Ure2 and inhibits formation of amyloid-like fibrils. J Biol Chem. 2007;282:11931-40 pubmed
    ..Overexpression of the type I Hsp40 member Ydj1 in yeast cells has been found to result in the loss of [URE3]...
  21. Sharma D, Stanley R, Masison D. Curing of yeast [URE3] prion by the Hsp40 cochaperone Ydj1p is mediated by Hsp70. Genetics. 2009;181:129-37 pubmed publisher
    URE3] is a prion of the yeast Ure2 protein. Hsp40 is a cochaperone that regulates Hsp70 chaperone activity. When overexpressed, the Hsp40 Ydj1p cures yeast of [URE3], but the Hsp40 Sis1p does not...
  22. Wu Y, Li J, Jin Z, Fu Z, Sha B. The crystal structure of the C-terminal fragment of yeast Hsp40 Ydj1 reveals novel dimerization motif for Hsp40. J Mol Biol. 2005;346:1005-11 pubmed
    The molecular chaperone Hsp40 functions as a dimer. The dimer formation is critical for Hsp40 molecular chaperone activity to facilitate Hsp70 to refold non-native polypeptides...
  23. Shaner L, Trott A, Goeckeler J, Brodsky J, Morano K. The function of the yeast molecular chaperone Sse1 is mechanistically distinct from the closely related hsp70 family. J Biol Chem. 2004;279:21992-2001 pubmed
    ..These data represent the first structure-function analysis of this abundant but ill defined chaperone, and establish several novel aspects of Sse1/Hsp110 function relative to Hsp70. ..
  24. Kimura Y, Yahara I, Lindquist S. Role of the protein chaperone YDJ1 in establishing Hsp90-mediated signal transduction pathways. Science. 1995;268:1362-5 pubmed
    ..A mutation in YDJ1, a member of the DnaJ chaperone family, was recovered in a synthetic-lethal screen with Hsp90 mutants...
  25. Lu Z, Cyr D. Protein folding activity of Hsp70 is modified differentially by the hsp40 co-chaperones Sis1 and Ydj1. J Biol Chem. 1998;273:27824-30 pubmed
    Specification of Hsp70 action in cellular protein metabolism may occur through the formation of specialized Hsp70:Hsp40 pairs...
  26. Borges J, Seraphim T, Mokry D, Almeida F, Cyr D, Ramos C. Identification of regions involved in substrate binding and dimer stabilization within the central domains of yeast Hsp40 Sis1. PLoS ONE. 2012;7:e50927 pubmed publisher
    ..eukaryotes, the DnaJ family can be divided into two main groups, Type I and Type II, represented by yeast cytosolic Ydj1 and Sis1, respectively...
  27. Nekrasov V, Smith M, Peak Chew S, Kilmartin J. Interactions between centromere complexes in Saccharomyces cerevisiae. Mol Biol Cell. 2003;14:4931-46 pubmed
    ..These results show an increasingly complex structure for the S. cerevisiae centromere and a probable conservation of structure between parts of the centromeres of S. cerevisiae and S. pombe. ..
  28. Chanet R, Heude M. Characterization of mutations that are synthetic lethal with pol3-13, a mutated allele of DNA polymerase delta in Saccharomyces cerevisiae. Curr Genet. 2003;43:337-50 pubmed
    ..transduction pathway (MKS1), in nuclear pore metabolism (SEH1), in protein degradation (DOC1) and in folding (YDJ1). Finally, mutations in three genes of unknown function were isolated (NBP35, DRE2, TAH18)...
  29. Vergés E, Colomina N, Garí E, Gallego C, Aldea M. Cyclin Cln3 is retained at the ER and released by the J chaperone Ydj1 in late G1 to trigger cell cycle entry. Mol Cell. 2007;26:649-62 pubmed
    ..Cln3 contains a chaperone-regulatory Ji domain that counteracts Ydj1, a J chaperone essential for ER release and nuclear accumulation of Cln3 in late G1...
  30. Hoseini H, Pandey S, Jores T, Schmitt A, Franz Wachtel M, Macek B, et al. The cytosolic cochaperone Sti1 is relevant for mitochondrial biogenesis and morphology. FEBS J. 2016;283:3338-52 pubmed publisher
    ..cells we identified the cytosolic chaperones Hsp70 (Ssa1) and Hsp90 (Hsp82) as well as their cochaperones, Sti1 and Ydj1, as putative cytosolic factors involved in mitochondrial protein import...
  31. Kapoor P, Bao Y, Xiao J, Luo J, Shen J, Persinger J, et al. Regulation of Mec1 kinase activity by the SWI/SNF chromatin remodeling complex. Genes Dev. 2015;29:591-602 pubmed publisher
    ..Together, these findings identify a novel regulator of Mec1 kinase activity and suggest that ATP-dependent chromatin remodeling complexes can regulate nonchromatin substrates such as a checkpoint kinase. ..
  32. Gemayel R, Chavali S, Pougach K, Legendre M, Zhu B, Boeynaems S, et al. Variable Glutamine-Rich Repeats Modulate Transcription Factor Activity. Mol Cell. 2015;59:615-27 pubmed publisher
    ..Thus, Q-rich repeats are dynamic functional domains that modulate a regulator's innate function, with the inherent risk of pathogenic repeat expansions. ..
  33. Zarzov P, Boucherie H, Mann C. A yeast heat shock transcription factor (Hsf1) mutant is defective in both Hsc82/Hsp82 synthesis and spindle pole body duplication. J Cell Sci. 1997;110 ( Pt 16):1879-91 pubmed
    ..We describe here our phenotypic analysis of two such mutants, hsf1-82 and ydj1-10, that affect the heat shock transcription factor and a yeast dnaj-like protein chaperone, respectively...
  34. Tutar L, Tutar Y. Ydj1 but not Sis1 stabilizes Hsp70 protein under prolonged stress in vitro. Biopolymers. 2008;89:171-4 pubmed
    Yeast cytosol has two important co-chaperons; Ydj1 and Sis1. Genetic experiments showed that Ydj1 is not essential for viability; however, cells lacking it grow very poorly at 30 degrees C or unable to grow at extreme temperatures...
  35. Youker R, Walsh P, Beilharz T, Lithgow T, Brodsky J. Distinct roles for the Hsp40 and Hsp90 molecular chaperones during cystic fibrosis transmembrane conductance regulator degradation in yeast. Mol Biol Cell. 2004;15:4787-97 pubmed
    ..Because Hsp70 function impacts CFTR biogenesis in yeast and mammals, we first sought ER-associated Hsp40 cochaperones involved in CFTR maturation...
  36. Huyer G, Piluek W, Fansler Z, Kreft S, Hochstrasser M, Brodsky J, et al. Distinct machinery is required in Saccharomyces cerevisiae for the endoplasmic reticulum-associated degradation of a multispanning membrane protein and a soluble luminal protein. J Biol Chem. 2004;279:38369-78 pubmed
    ..Furthermore, the cytosolic Hsp70 chaperone Ssa1p and the Hsp40 co-chaperones Ydj1p and Hlj1p are important in ERAD of Ste6p*, whereas the ER luminal chaperone Kar2p is ..
  37. Duennwald M, Echeverria A, Shorter J. Small heat shock proteins potentiate amyloid dissolution by protein disaggregases from yeast and humans. PLoS Biol. 2012;10:e1001346 pubmed publisher
    ..sHsp binding destabilizes Sup35 prions and promotes their disaggregation by Hsp104, Hsp70, and Hsp40. In yeast, Hsp26 or Hsp42 overexpression prevents [PSI+] induction, cures [PSI+], and potentiates [PSI+]-curing by ..
  38. Qiu H, Chereji R, Hu C, Cole H, Rawal Y, Clark D, et al. Genome-wide cooperation by HAT Gcn5, remodeler SWI/SNF, and chaperone Ydj1 in promoter nucleosome eviction and transcriptional activation. Genome Res. 2016;26:211-25 pubmed publisher
    ..Examining four target genes in a panel of mutants indicated that SWI/SNF, Gcn5, the Hsp70 cochaperone Ydj1, and chromatin-associated factor Yta7 are required downstream from Gcn4 binding, whereas Asf1/Rtt109, Nap1, RSC, ..
  39. Lee R, Brunette S, Puente L, Megeney L. Metacaspase Yca1 is required for clearance of insoluble protein aggregates. Proc Natl Acad Sci U S A. 2010;107:13348-53 pubmed publisher
    ..Together, our results show that Yca1 contributes to the fitness and adaptability of growing yeast through an aggregate remodeling activity. ..
  40. Zhang W, Zhao H, Xue C, Xiong X, Yao X, Li X, et al. Enhanced secretion of heterologous proteins in Pichia pastoris following overexpression of Saccharomyces cerevisiae chaperone proteins. Biotechnol Prog. 2006;22:1090-5 pubmed
    ..The Hsp70 and Hsp40 chaperone families in the cytoplasm or in ER importantly regulate the folding and secretion of heterologous ..
  41. Fang N, Chan G, Zhu M, Comyn S, Persaud A, Deshaies R, et al. Rsp5/Nedd4 is the main ubiquitin ligase that targets cytosolic misfolded proteins following heat stress. Nat Cell Biol. 2014;16:1227-37 pubmed publisher
    ..We found that ubiquitylation of heat-induced substrates requires the Hsp40 co-chaperone Ydj1 that is further associated with Rsp5 upon heat shock...
  42. Malinovska L, Kroschwald S, Munder M, Richter D, Alberti S. Molecular chaperones and stress-inducible protein-sorting factors coordinate the spatiotemporal distribution of protein aggregates. Mol Biol Cell. 2012;23:3041-56 pubmed publisher
    ..Our model suggests that protein aggregation is not a haphazard process but rather an orchestrated cellular response that adjusts the flux of misfolded proteins to the capacities of the protein quality control system. ..
  43. Cyr D, Douglas M. Differential regulation of Hsp70 subfamilies by the eukaryotic DnaJ homologue YDJ1. J Biol Chem. 1994;269:9798-804 pubmed
    ..Ssa1p complexes. We propose that intramolecular communication between the polypeptide binding, ATPase and DnaJ regulatory domains on Ssa1p plays a role in the regulation of chaperone activity. ..
  44. King C, Eisenberg E, Greene L. Polymerization of 70-kDa heat shock protein by yeast DnaJ in ATP. J Biol Chem. 1995;270:22535-40 pubmed
    ..The yeast DnaJ homolog, YDJ1, has also been shown to interact with yeast hsp70, although the function of this interaction is unknown...
  45. Takano A, Kajita T, Mochizuki M, Endo T, Yoshihisa T. Cytosolic Hsp70 and co-chaperones constitute a novel system for tRNA import into the nucleus. elife. 2015;4: pubmed publisher
    ..These results unveil a novel function of the Ssa2p system as a tRNA carrier for nuclear import by a novel mode of substrate recognition. Such Ssa2p-mediated tRNA import likely contributes to quality control of cytosolic tRNAs. ..
  46. Chang Y, Chuang Y, Ho Y, Cheng M, Sun Y, Hsiao C, et al. Crystal structure of Get4-Get5 complex and its interactions with Sgt2, Get3, and Ydj1. J Biol Chem. 2010;285:9962-70 pubmed publisher
    ..Moreover, GET3, GET4, and GET5 interacted genetically with molecular chaperone YDJ1, suggesting that chaperones might also be involved in the insertion of tail-anchored proteins.
  47. Tenge V, Knowles J, Johnson J. The ribosomal biogenesis protein Utp21 interacts with Hsp90 and has differing requirements for Hsp90-associated proteins. PLoS ONE. 2014;9:e92569 pubmed publisher
  48. Hildebrandt E, Cheng M, Zhao P, Kim J, Wells L, Schmidt W. A shunt pathway limits the CaaX processing of Hsp40 Ydj1p and regulates Ydj1p-dependent phenotypes. elife. 2016;5: pubmed publisher
    ..These results expand on the complexity of protein isoprenylation and highlight the impact of post-isoprenylation events in regulating the function of Ydj1p and perhaps other CaaX proteins. ..
  49. Nillegoda N, Stank A, Malinverni D, Alberts N, Szlachcic A, Barducci A, et al. Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes. elife. 2017;6: pubmed publisher
    ..This fundamental change in J-protein biology during the prokaryote-to-eukaryote transition allows for increased fine-tuning and broadening of Hsp70 function in eukaryotes. ..
  50. Moriel Carretero M, Tous C, Aguilera A. Control of the function of the transcription and repair factor TFIIH by the action of the cochaperone Ydj1. Proc Natl Acad Sci U S A. 2011;108:15300-5 pubmed publisher
    ..We found that the cochaperone Ydj1 is required for NER and that Ydj1 guarantees TFIIH stoichiometry...
  51. Qian X, Li Z, Sha B. Cloning, expression, purification and preliminary X-ray crystallographic studies of yeast Hsp40 Sis1 complexed with Hsp70 Ssa1 C-terminal lid domain. Acta Crystallogr D Biol Crystallogr. 2001;57:748-50 pubmed
    ..Heat-shock protein 40 (Hsp40) transiently interacts with Hsp70 and facilitates Hsp70 functions in these processes within cells...
  52. Meacham G, Browne B, Zhang W, Kellermayer R, Bedwell D, Cyr D. Mutations in the yeast Hsp40 chaperone protein Ydj1 cause defects in Axl1 biogenesis and pro-a-factor processing. J Biol Chem. 1999;274:34396-402 pubmed
    The heat shock protein (Hsp) 70/Hsp40 chaperone system plays an essential role in cell physiology, but few of its in vivo functions are known...
  53. Jones M, Bachant J, Castillo A, Giddings T, Winey M. Yeast Dam1p is required to maintain spindle integrity during mitosis and interacts with the Mps1p kinase. Mol Biol Cell. 1999;10:2377-91 pubmed
  54. Silva J, Borges J, Cyr D, Ramos C, Torriani I. Central domain deletions affect the SAXS solution structure and function of yeast Hsp40 proteins Sis1 and Ydj1. BMC Struct Biol. 2011;11:40 pubmed publisher
    Ydj1 and Sis1 are structurally and functionally distinct Hsp40 proteins of the yeast cytosol. Sis1 is an essential gene whereas the ydj1 gene is essential for growth at elevated temperatures and cannot complement sis1 gene deletion...
  55. Wright C, Fewell S, Sullivan M, Pipas J, Watkins S, Brodsky J. The Hsp40 molecular chaperone Ydj1p, along with the protein kinase C pathway, affects cell-wall integrity in the yeast Saccharomyces cerevisiae. Genetics. 2007;175:1649-64 pubmed
    Molecular chaperones, such as Hsp40, regulate cellular processes by aiding in the folding, localization, and activation of multi-protein machines...
  56. Atencio D, Yaffe M. MAS5, a yeast homolog of DnaJ involved in mitochondrial protein import. Mol Cell Biol. 1992;12:283-91 pubmed
    The nuclear mas5 mutation causes temperature-sensitive growth and defects in mitochondrial protein import at the nonpermissive temperature in the yeast Saccharomyces cerevisiae...
  57. Kota P, Summers D, Ren H, Cyr D, Dokholyan N. Identification of a consensus motif in substrates bound by a Type I Hsp40. Proc Natl Acad Sci U S A. 2009;106:11073-8 pubmed publisher
    ..Molecular chaperones of the Hsp40 family (Escherichia coli DnaJ homologs) recognize misfolded disease proteins and suppress the accumulation of ..
  58. NEAL S, Mak R, Bennett E, HAMPTON R. A Cdc48 "Retrochaperone" Function Is Required for the Solubility of Retrotranslocated, Integral Membrane Endoplasmic Reticulum-associated Degradation (ERAD-M) Substrates. J Biol Chem. 2017;292:3112-3128 pubmed publisher
    ..Thus, in addition to the long known ATP-dependent extraction of ERAD substrates during retrotranslocation, the Cdc48 complex is generally and critically needed for the solubility of retrotranslocated ERAD-M intermediates. ..
  59. Hamdan N, Kritsiligkou P, Grant C. ER stress causes widespread protein aggregation and prion formation. J Cell Biol. 2017;216:2295-2304 pubmed publisher
    ..The onset of ER stress is known to correlate with various disease processes, and our data indicate that widespread amorphous and amyloid protein aggregation is an unanticipated outcome of such stress. ..
  60. Mirzaei H, Rogers R, Grimes B, Eng J, Aderem A, Aebersold R. Characterizing the connectivity of poly-ubiquitin chains by selected reaction monitoring mass spectrometry. Mol Biosyst. 2010;6:2004-14 pubmed publisher
    ..cerevisiae. We then applied the method to detect toxin induced changes in the poly-ubiquitination profile in complex and enriched protein samples. ..
  61. Krzewska J, Melki R. Molecular chaperones and the assembly of the prion Sup35p, an in vitro study. EMBO J. 2006;25:822-33 pubmed
    ..Hsp82p, Ssa1p and Sis1p, individually, do not affect assembly. In contrast, Ssa1p together with either of its Hsp40 cochaperones blocks Sup35p polymerization...
  62. Lan C, Lee H, Tang S, Zhang L. A novel mode of chaperone action: heme activation of Hap1 by enhanced association of Hsp90 with the repressed Hsp70-Hap1 complex. J Biol Chem. 2004;279:27607-12 pubmed
    ..Heme enhances the interaction between Hap1 and Hsp90. In vivo, defective Ssa, Ydj1, or Sro9 function causes Hap1 derepression in the absence of heme, whereas defective Hsp90 function causes reduced ..
  63. Reidy M, Sharma R, Shastry S, Roberts B, Albino Flores I, Wickner S, et al. Hsp40s specify functions of Hsp104 and Hsp90 protein chaperone machines. PLoS Genet. 2014;10:e1004720 pubmed publisher
    Hsp100 family chaperones of microorganisms and plants cooperate with the Hsp70/Hsp40/NEF system to resolubilize and reactivate stress-denatured proteins...
  64. Oka M, Nakai M, Endo T, Lim C, Kimata Y, Kohno K. Loss of Hsp70-Hsp40 chaperone activity causes abnormal nuclear distribution and aberrant microtubule formation in M-phase of Saccharomyces cerevisiae. J Biol Chem. 1998;273:29727-37 pubmed
    ..In support of this idea, a ydj1 null mutant at the restrictive temperature was found to exhibit more prominent phenotypes than ssa1-134...
  65. Li J, Sha B. Structure-based mutagenesis studies of the peptide substrate binding fragment of type I heat-shock protein 40. Biochem J. 2005;386:453-60 pubmed
    Ydj1 is the major type I Hsp40 (heat-shock protein 40) family member in yeast. Ydj1 can pair with yeast Hsp70 Ssa1 to facilitate protein translocation and protein folding...
  66. Wang F, Brown E, Mak G, Zhuang J, Denic V. A chaperone cascade sorts proteins for posttranslational membrane insertion into the endoplasmic reticulum. Mol Cell. 2010;40:159-71 pubmed publisher
    ..Thus, ER-bound TA proteins are sorted at the top of a TMD chaperone cascade that culminates with the formation of Get3-TA protein complexes, which are recruited to the ER membrane for insertion. ..
  67. Yaglom J, Goldberg A, Finley D, Sherman M. The molecular chaperone Ydj1 is required for the p34CDC28-dependent phosphorylation of the cyclin Cln3 that signals its degradation. Mol Cell Biol. 1996;16:3679-84 pubmed
    ..Here we demonstrate that the molecular chaperone Ydj1, a DnaJ homolog, is required for this phosphorylation...