YCF1

Summary

Gene Symbol: YCF1
Description: ATP-binding cassette glutathione S-conjugate transporter YCF1
Alias: ATP-binding cassette glutathione S-conjugate transporter YCF1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Mielniczki Pereira A, Schuch A, Bonatto D, Cavalcante C, Vaitsman D, Riger C, et al. The role of the yeast ATP-binding cassette Ycf1p in glutathione and cadmium ion homeostasis during respiratory metabolism. Toxicol Lett. 2008;180:21-7 pubmed publisher
    ..This tolerance is probably related to the high content of GSH present in ycf1Delta mutant. The expression of YCF1 promoter in the wild-type strain is naturally down-regulated after the transition from fermentative to respiratory ..
  2. Nagy Z, Montigny C, Leverrier P, Yeh S, Goffeau A, Garrigos M, et al. Role of the yeast ABC transporter Yor1p in cadmium detoxification. Biochimie. 2006;88:1665-71 pubmed
    Growth of yeast strains, either deleted for the vacuolar ABC transporter Ycf1 or deleted for the plasma membrane ABC transporter Yor1p or overexpressing Yor1p, were compared for their sensitivity to cadmium...
  3. Bouganim N, David J, Wysocki R, Ramotar D. Yap1 overproduction restores arsenite resistance to the ABC transporter deficient mutant ycf1 by activating ACR3 expression. Biochem Cell Biol. 2001;79:441-8 pubmed
    b>Ycf1 and Acr3 are transporters that have been previously shown to protect Saccharomyces cerevisiae cells from the toxic effects of arsenite...
  4. Sasser T, Lawrence G, Karunakaran S, Brown C, Fratti R. The yeast ATP-binding cassette (ABC) transporter Ycf1p enhances the recruitment of the soluble SNARE Vam7p to vacuoles for efficient membrane fusion. J Biol Chem. 2013;288:18300-10 pubmed publisher
    ..We found that deletion of YCF1 attenuated in vitro vacuole fusion by up to 40% relative to wild-type vacuoles...
  5. Petrovic S, Pascolo L, Gallo R, Cupelli F, Ostrow J, Goffeau A, et al. The products of YCF1 and YLL015w (BPT1) cooperate for the ATP-dependent vacuolar transport of unconjugated bilirubin in Saccharomyces cerevisiae. Yeast. 2000;16:561-71 pubmed
    ..showed an ATP-dependent, saturative transport of unconjugated bilirubin (UCB) that was reduced by 60% and 40% in YCF1 and YLL015w-deleted cells, respectively; the double deletant showed no UCB uptake...
  6. Wei W, Smith N, Wu X, Kim H, Seravalli J, Khalimonchuk O, et al. YCF1-mediated cadmium resistance in yeast is dependent on copper metabolism and antioxidant enzymes. Antioxid Redox Signal. 2014;21:1475-89 pubmed publisher
  7. Gomes D, Fragoso L, Riger C, Panek A, Eleutherio E. Regulation of cadmium uptake by Saccharomyces cerevisiae. Biochim Biophys Acta. 2002;1573:21-5 pubmed
    ..Moreover, the deletion of YCF1 (which encodes a vacuolar glutathione S-conjugate pump) impaired the transport of this metal significantly...
  8. Sharma K, Mason D, Liu G, Rea P, Bachhawat A, Michaelis S. Localization, regulation, and substrate transport properties of Bpt1p, a Saccharomyces cerevisiae MRP-type ABC transporter. Eukaryot Cell. 2002;1:391-400 pubmed
    ..In addition, the expression patterns of YCF1 and BPT1 differ significantly...
  9. Di Y, Tamás M. Regulation of the arsenic-responsive transcription factor Yap8p involves the ubiquitin-proteasome pathway. J Cell Sci. 2007;120:256-64 pubmed
    ..In this way, regulated degradation contributes to Yap8p control. ..

More Information

Publications27

  1. Srikanth C, Chakraborti A, Bachhawat A. Acetaminophen toxicity and resistance in the yeast Saccharomyces cerevisiae. Microbiology. 2005;151:99-111 pubmed
    ..In contrast, resistance mediated by Pdr1p/Pdr3p did not require a functional Yap1p, and revealed a distinct hierarchy in the resistance to acetaminophen...
  2. Sharma K, Kaur R, Bachhawat A. The glutathione-mediated detoxification pathway in yeast: an analysis using the red pigment that accumulates in certain adenine biosynthetic mutants of yeasts reveals the involvement of novel genes. Arch Microbiol. 2003;180:108-17 pubmed
    ..By contrast, two other previously characterized genes, the oxidative stress transcription factor gene, SKN7, and the yeast caesin protein kinase gene, YCK1, of S. cerevisiae do participate in this pathway. ..
  3. Tommasini R, Evers R, Vogt E, Mornet C, Zaman G, Schinkel A, et al. The human multidrug resistance-associated protein functionally complements the yeast cadmium resistance factor 1. Proc Natl Acad Sci U S A. 1996;93:6743-8 pubmed
    A Saccharomyces cerevisiae strain with a disrupted yeast cadmium resistance factor (YCF1) gene (DTY168) is hypersensitive to cadmium...
  4. Ghosh M, Shen J, Rosen B. Pathways of As(III) detoxification in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1999;96:5001-6 pubmed
    ..Disruption of either the ACR3 or YCF1 gene results in sensitivity to arsenite and disruption of both genes produces additive hypersensitivity...
  5. Kawabata K, Mitsui K, Uno T, Tamura K, Tsurugi K. Protein interactions of Gts1p of Saccharomyces cerevisiae throughout a region similar to a cytoplasmic portion of some ATP-binding cassette transporters. Eur J Biochem. 1999;259:112-9 pubmed
    ..These results suggest that Gts1p interacts with some ABC transporters through the binding site overlapping that of homodimerization and modulates their activity...
  6. Paumi C, Pickin K, Jarrar R, Herren C, Cowley S. Ycf1p attenuates basal level oxidative stress response in Saccharomyces cerevisiae. FEBS Lett. 2012;586:847-53 pubmed publisher
    ..Our results suggest that during acute salt stress increased Sod1p, Sod2p and Ctt1p activity is the main compensatory for the loss in Ycf1p function that results from reduced Ycf1p-dependent recycling of cellular GSH levels...
  7. Preveral S, Ansoborlo E, Mari S, Vavasseur A, Forestier C. Metal(loid)s and radionuclides cytotoxicity in Saccharomyces cerevisiae. Role of YCF1, glutathione and effect of buthionine sulfoximine. Biochimie. 2006;88:1651-63 pubmed
    ..The yeast cadmium factor 1 (YCF1) is an ATP-Binding Cassette transporter mediating the glutathione detoxification of heavy metals...
  8. Maciaszczyk Dziubinska E, Migdal I, Migocka M, Bocer T, Wysocki R. The yeast aquaglyceroporin Fps1p is a bidirectional arsenite channel. FEBS Lett. 2010;584:726-32 pubmed publisher
    ..Moreover, deletion of FPS1 gene resulted in arsenate sensitivity. Finally, transport experiments revealed that Fps1p in concert with the arsenite transporter Acr3p mediates arsenite efflux...
  9. Paumi C, Menendez J, Arnoldo A, Engels K, Iyer K, Thaminy S, et al. Mapping protein-protein interactions for the yeast ABC transporter Ycf1p by integrated split-ubiquitin membrane yeast two-hybrid analysis. Mol Cell. 2007;26:15-25 pubmed
    ..Our study provides proof of principle that iMYTH is an ideal methodology to identify physiological interactors and regulators of ABC transporters and other yeast transmembrane proteins...
  10. Pickin K, Ezenwajiaku N, Overcash H, Sethi M, Knecht M, Paumi C. Suppression of Ycf1p function by Cka1p-dependent phosphorylation is attenuated in response to salt stress. FEMS Yeast Res. 2010;10:839-57 pubmed publisher
    ..In conclusion, our work demonstrates a novel biochemical role for Cka1p regulation of Ycf1p function in the cellular response of yeast to salt stress...
  11. Morgan B, Ezerina D, Amoako T, Riemer J, Seedorf M, Dick T. Multiple glutathione disulfide removal pathways mediate cytosolic redox homeostasis. Nat Chem Biol. 2013;9:119-25 pubmed publisher
    ..that is not immediately reduced in the cytosol is rapidly transported into the vacuole by the ABC-C transporter Ycf1. The amount of whole-cell GSSG is entirely dependent on Ycf1 and uninformative about the cytosolic glutathione pool...
  12. Mielniczki Pereira A, Hahn A, Bonatto D, Riger C, Eleutherio E, Henriques J. New insights into the Ca2+-ATPases that contribute to cadmium tolerance in yeast. Toxicol Lett. 2011;207:104-11 pubmed publisher
    ..Herein, we showed that inactivation of the PMR1 gene, alone or simultaneously with YCF1, delayed initial Cd(2+) capture compared to wild-type (WT) cells...
  13. Wysocki R, Fortier P, Maciaszczyk E, Thorsen M, Leduc A, Odhagen A, et al. Transcriptional activation of metalloid tolerance genes in Saccharomyces cerevisiae requires the AP-1-like proteins Yap1p and Yap8p. Mol Biol Cell. 2004;15:2049-60 pubmed
    ..We conclude that Yap1p and Yap8p mediate tolerance by controlling separate subsets of detoxification genes and propose that the two AP-1-like proteins respond to metalloids through distinct mechanisms. ..
  14. Paumi C, Chuk M, Chevelev I, Stagljar I, Michaelis S. Negative regulation of the yeast ABC transporter Ycf1p by phosphorylation within its N-terminal extension. J Biol Chem. 2008;283:27079-88 pubmed publisher
    ..Activity is restored to the wild-type level for Ycf1-S251E...
  15. Ishikawa T, Li Z, Lu Y, Rea P. The GS-X pump in plant, yeast, and animal cells: structure, function, and gene expression. Biosci Rep. 1997;17:189-207 pubmed
    ..The molecular structure and function of GS-X pumps encoded by MRP, cMOAT, YCF1, and AtMRP genes, have been conserved throughout molecular evolution...
  16. Maciaszczyk Dziubinska E, Wawrzycka D, Sloma E, Migocka M, Wysocki R. The yeast permease Acr3p is a dual arsenite and antimonite plasma membrane transporter. Biochim Biophys Acta. 2010;1798:2170-5 pubmed publisher
    ..Finally, transport experiments confirmed that Acr3p indeed mediates efflux of antimonite and thus possesses a dual arsenite and antimonite specificity. ..
  17. Lee M, Singh K, Snider J, Shenoy A, Paumi C, Stagljar I, et al. The Rho1 GTPase acts together with a vacuolar glutathione S-conjugate transporter to protect yeast cells from oxidative stress. Genetics. 2011;188:859-70 pubmed publisher
    ..Rho1 associates with Ycf1, a vacuolar glutathione S-conjugate transporter, which is important for heavy metal detoxification in yeast...
  18. Gueldry O, Lazard M, Delort F, Dauplais M, Grigoras I, Blanquet S, et al. Ycf1p-dependent Hg(II) detoxification in Saccharomyces cerevisiae. Eur J Biochem. 2003;270:2486-96 pubmed
    In Saccharomyces cerevisiae, disruption of the YCF1 gene increases the sensitivity of cell growth to mercury...