Gene Symbol: WSS1
Description: metalloendopeptidase WSS1
Alias: metalloendopeptidase WSS1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Biggins S, Bhalla N, Chang A, Smith D, Murray A. Genes involved in sister chromatid separation and segregation in the budding yeast Saccharomyces cerevisiae. Genetics. 2001;159:453-70 pubmed
    ..We also report an initial characterization of phenotypes associated with the SMT3/SUMO gene and the isolation of WSS1, a high-copy smt3 suppressor.
  2. Meednu N, Hoops H, D Silva S, Pogorzala L, Wood S, Farkas D, et al. The spindle positioning protein Kar9p interacts with the sumoylation machinery in Saccharomyces cerevisiae. Genetics. 2008;180:2033-55 pubmed publisher
    ..We propose a model in which sumoylation regulates spindle positioning by restricting Kar9p to one SPB. These findings raise the possibility that sumoylation could regulate other microtubule-dependent processes. ..
  3. Mullen J, Chen C, Brill S. Wss1 is a SUMO-dependent isopeptidase that interacts genetically with the Slx5-Slx8 SUMO-targeted ubiquitin ligase. Mol Cell Biol. 2010;30:3737-48 pubmed publisher
    ..b>WSS1, a high-copy-number suppressor of a mutant SUMO, was implicated in this pathway because it shares phenotypes with ..
  4. O Neill B, Hanway D, Winzeler E, Romesberg F. Coordinated functions of WSS1, PSY2 and TOF1 in the DNA damage response. Nucleic Acids Res. 2004;32:6519-30 pubmed
    ..Disruption of WSS1 resulted in synergistic increases in UV sensitivity with null mutants of genes involved in recombination (RAD52) ..
  5. Lopez Mosqueda J, Maddi K, Prgomet S, Kalayil S, Marinovic Terzic I, Terzic J, et al. SPRTN is a mammalian DNA-binding metalloprotease that resolves DNA-protein crosslinks. elife. 2016;5: pubmed publisher
    ..resolving cytotoxic DNA-protein crosslinks (DPCs)- a function that had only been attributed to the metalloprotease Wss1 in budding yeast. We provide genetic evidence that SPRTN and Wss1 function distinctly in vivo to resolve DPCs...
  6. Sharma P, Mullen J, Li M, Zaratiegui M, Bunting S, Brill S. A Lysine Desert Protects a Novel Domain in the Slx5-Slx8 SUMO Targeted Ub Ligase To Maintain Sumoylation Levels in Saccharomyces cerevisiae. Genetics. 2017;206:1807-1821 pubmed publisher
    ..Yeast cells lacking the repair factors TDP1 and WSS1 are synthetically lethal due to their redundant roles in removing Top1-DNA covalent complexes (Top1ccs)...
  7. Birrell G, Giaever G, Chu A, Davis R, Brown J. A genome-wide screen in Saccharomyces cerevisiae for genes affecting UV radiation sensitivity. Proc Natl Acad Sci U S A. 2001;98:12608-13 pubmed
  8. Bialkowska A, Kurlandzka A. Proteins interacting with Lin 1p, a putative link between chromosome segregation, mRNA splicing and DNA replication in Saccharomyces cerevisiae. Yeast. 2002;19:1323-33 pubmed
    ..round of two-hybrid assay with Lin1p as the bait resulted in the identification of six proteins: Prp8, Slx5, Siz2, Wss1, Rfc1 and YIL149w...
  9. Van Heusden G, Steensma H. The Saccharomyces cerevisiae Wss1 protein is only present in mother cells. FEMS Microbiol Lett. 2008;282:100-4 pubmed publisher
    The Saccharomyces cerevisiae WSS1 (Weak Suppressor of Smt3) gene has initially been identified as a multicopy suppressor of a mutation in SMT3 encoding the small ubiquitin-like modifier...

More Information


  1. Shirai C, Mizuta K. SUMO mediates interaction of Ebp2p, the yeast homolog of Epstein-Barr virus nuclear antigen 1-binding protein 2, with a RING finger protein Ris1p. Biosci Biotechnol Biochem. 2008;72:1881-6 pubmed
    ..A SUMO-binding motif of Ris1p was required for interaction with Ebp2p. These results suggest that SUMO mediates the interaction between Ebp2p and SUMO related proteins and that Ebp2p switches its interaction partners via sumoylation. ..
  2. Mullen J, Das M, Brill S. Genetic evidence that polysumoylation bypasses the need for a SUMO-targeted Ub ligase. Genetics. 2011;187:73-87 pubmed publisher
    ..In support of this latter possibility we find that the WSS1 isopeptidase is required for suppression by ulp2?.
  3. Alonso A, D Silva S, Rahman M, Meluh P, Keeling J, Meednu N, et al. The yeast homologue of the microtubule-associated protein Lis1 interacts with the sumoylation machinery and a SUMO-targeted ubiquitin ligase. Mol Biol Cell. 2012;23:4552-66 pubmed publisher
    ..Both Pac1p and Bik1p interact with the STUbL Nis1p-Ris1p and the protease Wss1p. Strains deleted for RIS1 or WSS1 accumulate Pac1p conjugates...
  4. Stingele J, Schwarz M, Bloemeke N, Wolf P, Jentsch S. A DNA-dependent protease involved in DNA-protein crosslink repair. Cell. 2014;158:327-338 pubmed publisher
    ..Here, we identify the metalloprotease Wss1 as being crucial for cell survival upon exposure to formaldehyde and topoisomerase 1-dependent DNA damage...
  5. Muñoz Galvan S, Jimeno S, Rothstein R, Aguilera A. Histone H3K56 acetylation, Rad52, and non-DNA repair factors control double-strand break repair choice with the sister chromatid. PLoS Genet. 2013;9:e1003237 pubmed publisher
    ..These include the SUMO/Ub-SUMO protease Wss1, the stress-response proteins Bud27 and Pdr10, the ADA histone acetyl-transferase complex proteins Ahc1 and Ada2, ..