Gene Symbol: WSC2
Description: Wsc2p
Alias: STA3, Wsc2p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Rajavel M, Philip B, Buehrer B, Errede B, Levin D. Mid2 is a putative sensor for cell integrity signaling in Saccharomyces cerevisiae. Mol Cell Biol. 1999;19:3969-76 pubmed
    ..We conclude that Hcs77 is dedicated to signaling cell wall stress during vegetative growth and that Mid2 participates in this signaling, but its primary role is in signaling wall stress during pheromone-induced morphogenesis...
  2. Ketela T, Green R, Bussey H. Saccharomyces cerevisiae mid2p is a potential cell wall stress sensor and upstream activator of the PKC1-MPK1 cell integrity pathway. J Bacteriol. 1999;181:3330-40 pubmed suppressed by overexpression of upstream elements of the cell integrity pathway, including PKC1, RHO1, WSC1, and WSC2. Mid2p and Wsc1p appear to have overlapping roles in maintaining cell integrity since mid2Delta wsc1Delta mutants ..
  3. Lodder A, Lee T, Ballester R. Characterization of the Wsc1 protein, a putative receptor in the stress response of Saccharomyces cerevisiae. Genetics. 1999;152:1487-99 pubmed
    Wsc1p, Wsc2p, Wsc3p, and Wsc4p, members of a novel protein family in the yeast Saccharomyces cerevisiae, are putative sensors or receptors in the stress response...
  4. Verna J, Lodder A, Lee K, Vagts A, Ballester R. A family of genes required for maintenance of cell wall integrity and for the stress response in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1997;94:13804-9 pubmed
    ..We have identified a family of genes that are putative regulators of this pathway. WSC1, WSC2, and WSC3 encode predicted integral membrane proteins with a conserved cysteine motif and a WSC1-green fluorescence ..
  5. Delley P, Hall M. Cell wall stress depolarizes cell growth via hyperactivation of RHO1. J Cell Biol. 1999;147:163-74 pubmed
    ..Thus, activated RHO1 can mediate both polarized and depolarized cell growth via the same effector, PKC1, suggesting that RHO1 may function as a rheostat rather than as a simple on-off switch. ..
  6. Nanduri J, Tartakoff A. The arrest of secretion response in yeast: signaling from the secretory path to the nucleus via Wsc proteins and Pkc1p. Mol Cell. 2001;8:281-9 pubmed
    ..The activity of the downstream kinase, Pkc1p, is also required; however, the Pkc1p MAP kinase cascade is not. sec mutants initiate compensatory transcriptional changes distinct from those of the unfolded protein response. ..
  7. Ito W, Li X, Irie K, Mizuno T, Irie K. RNA-binding protein Khd1 and Ccr4 deadenylase play overlapping roles in the cell wall integrity pathway in Saccharomyces cerevisiae. Eukaryot Cell. 2011;10:1340-7 pubmed publisher
    ..Our results presented here suggest that Khd1 and Ccr4 modulate a signal from Rho1 in the CWI pathway by regulating the expression of RhoGEF and RhoGAP. ..
  8. Leung A, Cajigas I, Jia P, Ezhkova E, Brickner J, Zhao Z, et al. Histone H2B ubiquitylation and H3 lysine 4 methylation prevent ectopic silencing of euchromatic loci important for the cellular response to heat. Mol Biol Cell. 2011;22:2741-53 pubmed publisher
    ..Our data reveal that H2B ubiquitylation/H3K4 methylation play an important role in limiting ectopic association of silencing proteins with euchromatic genes important for cell wall integrity and the response to heat. ..
  9. Valentini S, Casolari J, Oliveira C, Silver P, McBride A. Genetic interactions of yeast eukaryotic translation initiation factor 5A (eIF5A) reveal connections to poly(A)-binding protein and protein kinase C signaling. Genetics. 2002;160:393-405 pubmed
    ..allows growth of a tif51A-1 strain at high temperature; these genes include PAB1, PKC1, and PKC1 regulators WSC1, WSC2, and WSC3...

More Information


  1. Birkaya B, Maddi A, Joshi J, Free S, Cullen P. Role of the cell wall integrity and filamentous growth mitogen-activated protein kinase pathways in cell wall remodeling during filamentous growth. Eukaryot Cell. 2009;8:1118-33 pubmed publisher
    ..Components of the cell wall integrity pathway including the cell surface proteins Slg1p/Wsc1p, Wsc2p, Mid2p, and the mitogen-activated protein kinase (MAPK) Slt2p/Mpk1p contributed to multiple aspects of the FG ..
  2. Hosotani T, Koyama H, Uchino M, Miyakawa T, Tsuchiya E. PKC1, a protein kinase C homologue of Saccharomyces cerevisiae, participates in microtubule function through the yeast EB1 homologue, BIM1. Genes Cells. 2001;6:775-88 pubmed
    ..These results suggest that Pkc1p plays a role which is relevant to microtubule functions and that this role is mediated by a hitherto unknown PKC signalling pathway and by Bim1p ..
  3. Zu T, Verna J, Ballester R. Mutations in WSC genes for putative stress receptors result in sensitivity to multiple stress conditions and impairment of Rlm1-dependent gene expression in Saccharomyces cerevisiae. Mol Genet Genomics. 2001;266:142-55 pubmed
    ..The genes WSC1, WSC2 and WSC3 encode putative receptors that maintain cell wall integrity under conditions of heat stress...
  4. Imazu H, Sakurai H. Saccharomyces cerevisiae heat shock transcription factor regulates cell wall remodeling in response to heat shock. Eukaryot Cell. 2005;4:1050-6 pubmed
    ..Another class of suppressors encoded cell wall stress sensors, Wsc1, Wsc2, and Mid2, and the GDP/GTP exchange factor Rom2 that interacts with these cell wall sensors...
  5. Raboy B, Marom A, Dor Y, Kulka R. Heat-induced cell cycle arrest of Saccharomyces cerevisiae: involvement of the RAD6/UBC2 and WSC2 genes in its reversal. Mol Microbiol. 1999;32:729-39 pubmed
    ..Taken together the findings point to the existence of an unidentified heat stress-activated cell cycle checkpoint pathway, which is antagonized by Rad6p by a mechanism also involving Wsc2p.
  6. Wojda I, Bebelman J, Jakubowicz T, Siderius M. Thermosensitivity of the Saccharomyces cerevisiae gpp1gpp2 double deletion strain can be reduced by overexpression of genes involved in cell wall maintenance. Arch Microbiol. 2007;188:175-84 pubmed
    ..We also confirmed that the internal glycerol level changed in cells exposed to cell wall perturbation. ..
  7. Shepard K, Gerber A, Jambhekar A, Takizawa P, Brown P, Herschlag D, et al. Widespread cytoplasmic mRNA transport in yeast: identification of 22 bud-localized transcripts using DNA microarray analysis. Proc Natl Acad Sci U S A. 2003;100:11429-34 pubmed
    ..In contrast to findings in metazoans, the untranslated regions are dispensable for mRNA localization in yeast. This study reveals an unanticipated widespread use of RNA transport in budding yeast. ..
  8. Kosodo Y, Imai K, Hirata A, Noda Y, Takatsuki A, Adachi H, et al. Multicopy suppressors of the sly1 temperature-sensitive mutation in the ER-Golgi vesicular transport in Saccharomyces cerevisiae. Yeast. 2001;18:1003-14 pubmed
    ..encode a multifunctional suppressor, SSD1; (2) heat shock proteins, SSB1 and SSB2; (3) cell surface proteins, WSC1, WSC2 and MID2; (4) ER-Golgi transport proteins, USO1 and BET1; and (5) an as-yet-uncharacterized protein, HSD1 (high-..
  9. de La Fuente N, Portillo F. The cell wall integrity/remodeling MAPK cascade is involved in glucose activation of the yeast plasma membrane H(+)-ATPase. Biochim Biophys Acta. 2000;1509:189-94 pubmed
    ..In a genetic screening for multicopy suppressors of the rsp5 mutation, we identified the WSC2/YNL283c gene...
  10. Wilk S, Wittland J, Thywissen A, Schmitz H, Heinisch J. A block of endocytosis of the yeast cell wall integrity sensors Wsc1 and Wsc2 results in reduced fitness in vivo. Mol Genet Genomics. 2010;284:217-29 pubmed publisher
    ..We here address the relation of intracellular localization of the sensors Wsc1, Wsc2, and Mid2 to their turnover and signaling function...
  11. Córcoles Sáez I, Ballester Tomás L, de la Torre Ruiz M, Prieto J, Randez Gil F. Low temperature highlights the functional role of the cell wall integrity pathway in the regulation of growth in Saccharomyces cerevisiae. Biochem J. 2012;446:477-88 pubmed publisher
    ..Hence, cold signalling through the CWI pathway has distinct features and might mediate still unknown effectors and targets. ..
  12. Zanelli C, Valentini S. Pkc1 acts through Zds1 and Gic1 to suppress growth and cell polarity defects of a yeast eIF5A mutant. Genetics. 2005;171:1571-81 pubmed
    ..Taken together, these data strongly support the correlated involvement of Pkc1 and eIF5A in establishing actin polarity, which is essential for bud formation and G1/S transition in S. cerevisiae. ..
  13. Lottersberger F, Panza A, Lucchini G, Piatti S, Longhese M. The Saccharomyces cerevisiae 14-3-3 proteins are required for the G1/S transition, actin cytoskeleton organization and cell wall integrity. Genetics. 2006;173:661-75 pubmed
    ..Both the protein kinase C1 (Pkc1) and its upstream regulators Wsc2 and Mid2 were found to act as high dosage suppressors of bmh mutants' temperature sensitivity, indicating a ..