Gene Symbol: WHI3
Description: mRNA-binding protein WHI3
Alias: mRNA-binding protein WHI3
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Malcher M, Schladebeck S, Mösch H. The Yak1 protein kinase lies at the center of a regulatory cascade affecting adhesive growth and stress resistance in Saccharomyces cerevisiae. Genetics. 2011;187:717-30 pubmed publisher
    ..regulatory genes, which have not been integrated into regulatory networks, affect adhesive growth, including WHI3 encoding an RNA-binding protein and YAK1 coding for a dual-specificity tyrosine-regulated protein kinase...
  2. Nash R, Volpe T, Futcher B. Isolation and characterization of WHI3, a size-control gene of Saccharomyces cerevisiae. Genetics. 2001;157:1469-80 pubmed
    b>WHI3 is a gene affecting size control and cell cycle in the yeast Saccharomyces cerevisiae. The whi3 mutant has small cells, while extra doses of WHI3 produce large cells, and a large excess of WHI3 produces a lethal arrest in G1 phase...
  3. Gari E, Volpe T, Wang H, Gallego C, Futcher B, Aldea M. Whi3 binds the mRNA of the G1 cyclin CLN3 to modulate cell fate in budding yeast. Genes Dev. 2001;15:2803-8 pubmed
    Eukaryotic cells commit in G1 to a new mitotic cycle or to diverse differentiation processes. Here we show that Whi3 is a negative regulator of Cln3, a G1 cyclin that promotes transcription of many genes to trigger the G1/S transition in ..
  4. Wang H, Garí E, Vergés E, Gallego C, Aldea M. Recruitment of Cdc28 by Whi3 restricts nuclear accumulation of the G1 cyclin-Cdk complex to late G1. EMBO J. 2004;23:180-90 pubmed
    The G1 cyclin Cln3 is a key activator of cell-cycle entry in budding yeast. Here we show that Whi3, a negative G1 regulator of Cln3, interacts in vivo with the cyclin-dependent kinase Cdc28 and regulates its localization in the cell...
  5. Mizunuma M, Tsubakiyama R, Ogawa T, Shitamukai A, Kobayashi Y, Inai T, et al. Ras/cAMP-dependent protein kinase (PKA) regulates multiple aspects of cellular events by phosphorylating the Whi3 cell cycle regulator in budding yeast. J Biol Chem. 2013;288:10558-66 pubmed publisher
    ..In the budding yeast Saccharomyces cerevisiae, Whi3, a negative regulator of the G1 cyclins, has been identified as a positive regulator of cell size control and is ..
  6. Flick K, Wittenberg C. Multiple pathways for suppression of mutants affecting G1-specific transcription in Saccharomyces cerevisiae. Genetics. 2005;169:37-49 pubmed
    ..Together with additional characterization these findings indicate that multiple independent pathways are sufficient for proliferation in the absence of G(1)-specific transcriptional activators. ..
  7. Colomina N, Ferrezuelo F, Vergés E, Aldea M, Garí E. Whi3 regulates morphogenesis in budding yeast by enhancing Cdk functions in apical growth. Cell Cycle. 2009;8:1912-20 pubmed
    The Whi3 protein is associated with the endoplasmic reticulum, interacts with Cdc28, the budding-yeast Cdk, binds the mRNA of cyclin CLN3 and prevents accumulation of the Cdc28-Cln3 in the nucleus until late G(1)...
  8. Ragni E, Piberger H, Neupert C, García Cantalejo J, Popolo L, Arroyo J, et al. The genetic interaction network of CCW12, a Saccharomyces cerevisiae gene required for cell wall integrity during budding and formation of mating projections. BMC Genomics. 2011;12:107 pubmed publisher
    ..Among those are PFD1, WHI3, SRN2, PAC10, FEN1 and YDR417C, which have not been related to cell wall integrity before...
  9. Schladebeck S, Mösch H. The RNA-binding protein Whi3 is a key regulator of developmental signaling and ploidy in Saccharomyces cerevisiae. Genetics. 2013;195:73-86 pubmed publisher
    In Saccharomyces cerevisiae, the RNA-binding protein Whi3 controls cell cycle progression, biofilm formation, and stress response by post-transcriptional regulation of the Cdc28-Cln3 cyclin-dependent protein kinase and the dual-..

More Information


  1. Mizunuma M, Ogawa T, Koyama T, Shitamukai A, Tsubakiyama R, Komaruyama T, et al. Evidence of antagonistic regulation of restart from G(1) delay in response to osmotic stress by the Hog1 and Whi3 in budding yeast. Biosci Biotechnol Biochem. 2013;77:2002-7 pubmed
    ..We found that Whi3, a negative regulator of G1 cyclin, counteracted Hog1 in the restart from G1 delay caused by osmotic stress...
  2. Schlissel G, Krzyzanowski M, Caudron F, Barral Y, Rine J. Aggregation of the Whi3 protein, not loss of heterochromatin, causes sterility in old yeast cells. Science. 2017;355:1184-1187 pubmed publisher
    ..however, they were less sensitive to mating pheromone than were young cells because of age-dependent aggregation of Whi3, an RNA-binding protein controlling S-phase entry...
  3. Mösch H, Fink G. Dissection of filamentous growth by transposon mutagenesis in Saccharomyces cerevisiae. Genetics. 1997;145:671-84 pubmed
    ..targets of the filamentation signaling pathway, and we thereby identified 16 different genes, CDC39, STE12, TEC1, WHI3, NAB1, DBR1, CDC55, SRV2, TPM1, SPA2, BNI1, DFG5, DFG9, DFG10, BUD8 and DFG16, mutations that block filamentous ..
  4. Caudron F, Barral Y. A super-assembly of Whi3 encodes memory of deceptive encounters by single cells during yeast courtship. Cell. 2013;155:1244-57 pubmed publisher
    ..This acquired behavior depends on super-assembly and inactivation of the G1/S inhibitor Whi3, which liberates the G1 cyclin Cln3 from translational inhibition...
  5. Cai Y, Futcher B. Effects of the yeast RNA-binding protein Whi3 on the half-life and abundance of CLN3 mRNA and other targets. PLoS ONE. 2013;8:e84630 pubmed publisher
    b>Whi3 is an RNA binding protein known to bind the mRNA of the yeast G1 cyclin gene CLN3...