Vps9p

Summary

Gene Symbol: Vps9p
Description: guanine nucleotide exchange factor VPS9
Alias: VPL31, VPT9, guanine nucleotide exchange factor VPS9
Species: Saccharomyces cerevisiae S288c
Products:     Vps9p

Top Publications

  1. Hama H, Tall G, Horazdovsky B. Vps9p is a guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport. J Biol Chem. 1999;274:15284-91 pubmed
    ..Using both yeast two-hybrid assays and chemical cross-linking, we have identified another VPS gene product, Vps9p, that preferentially interacts with a mutant form of Vps21p-S21N that binds GDP but not GTP...
  2. Keren Kaplan T, Attali I, Motamedchaboki K, Davis B, Tanner N, Reshef Y, et al. Synthetic biology approach to reconstituting the ubiquitylation cascade in bacteria. EMBO J. 2012;31:378-90 pubmed publisher
    ..Characterization of several ubiquitylated proteins demonstrated the integrity, specificity and fidelity of the system, and revealed new biological findings. ..
  3. Shih S, Prag G, Francis S, Sutanto M, Hurley J, Hicke L. A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain. EMBO J. 2003;22:1273-81 pubmed
    ..Thus, we conclude that the CUE motif is an evolutionarily conserved monoubiquitin-binding domain that mediates intramolecular monoubiquitylation. ..
  4. Davies B, Topp J, Sfeir A, Katzmann D, Carney D, Tall G, et al. Vps9p CUE domain ubiquitin binding is required for efficient endocytic protein traffic. J Biol Chem. 2003;278:19826-33 pubmed
    ..The yeast Rab5 ortholog Vps21p is activated by its guanine nucleotide exchange factor Vps9p. Here we show that Vps9p binds ubiquitin and that the CUE domain is necessary and sufficient for this interaction...
  5. Paulsel A, Merz A, Nickerson D. Vps9 family protein Muk1 is the second Rab5 guanosine nucleotide exchange factor in budding yeast. J Biol Chem. 2013;288:18162-71 pubmed publisher
    ..Microscopy and overexpression experiments demonstrate that Vps9 and Muk1 have distinct localization determinants. These experiments establish Muk1 as the second Rab5 GEF in budding yeast. ..
  6. Raymond C, Howald Stevenson I, Vater C, Stevens T. Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell. 1992;3:1389-402 pubmed
    ..A good correlation between morphological phenotype and the extent of the CPY sorting defect was observed. ..
  7. Shideler T, Nickerson D, Merz A, Odorizzi G. Ubiquitin binding by the CUE domain promotes endosomal localization of the Rab5 GEF Vps9. Mol Biol Cell. 2015;26:1345-56 pubmed publisher
    ..These results indicate that ubiquitin binding by the CUE domain promotes Vps9 function in endolysosomal membrane trafficking via promotion of localization. ..
  8. Xie P, Zhang M, He S, Lu K, Chen Y, Xing G, et al. The covalent modifier Nedd8 is critical for the activation of Smurf1 ubiquitin ligase in tumorigenesis. Nat Commun. 2014;5:3733 pubmed publisher
    ..These findings provide evidence that neddylation is important in HECT ubiquitin ligase activation and shed new light on the tumour-promoting role of Smurf1...
  9. Bridges D, Fisher K, Zolov S, Xiong T, Inoki K, Weisman L, et al. Rab5 proteins regulate activation and localization of target of rapamycin complex 1. J Biol Chem. 2012;287:20913-21 pubmed publisher
    ..Additionally, regulation of PI(3)P synthesis by Rab5 and Vps21 is essential for TORC1 function in both contexts. ..

More Information

Publications22

  1. Cabrera M, Arlt H, Epp N, Lachmann J, Griffith J, Perz A, et al. Functional separation of endosomal fusion factors and the class C core vacuole/endosome tethering (CORVET) complex in endosome biogenesis. J Biol Chem. 2013;288:5166-75 pubmed publisher
    ..the ultrastructure, CORVET mutants were most similar to deletions of the Rab Vps21 and its guanine nucleotide exchange factor Vps9 and different from vac1 deletion, indicating separate functions...
  2. Kang R, Daniels C, Francis S, Shih S, Salerno W, Hicke L, et al. Solution structure of a CUE-ubiquitin complex reveals a conserved mode of ubiquitin binding. Cell. 2003;113:621-30 pubmed
    ..Comparative modeling suggests that the UBA domain interacts analogously with ubiquitin. The structure of the CUE-ubiquitin complex may thus serve as a paradigm for ubiquitin recognition and signaling by ubiquitin binding proteins. ..
  3. Morimoto Y, Tani M. Synthesis of mannosylinositol phosphorylceramides is involved in maintenance of cell integrity of yeast Saccharomyces cerevisiae. Mol Microbiol. 2015;95:706-22 pubmed publisher
    ..These results indicated that loss of MIPC synthesis causes impairment of cell integrity, and this effect is enhanced by impaired synthesis of mannan-type N-glycans. ..
  4. Prag G, Misra S, Jones E, Ghirlando R, Davies B, Horazdovsky B, et al. Mechanism of ubiquitin recognition by the CUE domain of Vps9p. Cell. 2003;113:609-20 pubmed
    ..The 2.3 A structure of the Vps9p-CUE domain is a dimeric domain-swapped variant of the ubiquitin binding UBA domain. The 1...
  5. Burd C, Mustol P, Schu P, Emr S. A yeast protein related to a mammalian Ras-binding protein, Vps9p, is required for localization of vacuolar proteins. Mol Cell Biol. 1996;16:2369-77 pubmed
    ..novel reticular membrane structures as well as an accumulation of 40- to 50-nm-diameter vesicles, suggesting that Vps9p may be required for the consumption of transport vesicles containing vacuolar protein precursors...
  6. Kucharczyk R, Hoffman Sommer M, Piekarska I, von Mollard G, Rytka J. The Saccharomyces cerevisiae protein Ccz1p interacts with components of the endosomal fusion machinery. FEMS Yeast Res. 2009;9:565-73 pubmed publisher
    ..The genes MON1 and YPT7 also interact genetically with PEP12. These results suggest that the Ccz1p-Mon1p-Ypt7p complex is involved in fusion of transport vesicles to multiple target membranes in yeast cells. ..
  7. Nakayasu E, Ansong C, Brown J, Yang F, Lopez Ferrer D, Qian W, et al. Evaluation of selected binding domains for the analysis of ubiquitinated proteomes. J Am Soc Mass Spectrom. 2013;24:1214-23 pubmed publisher
    ..This approach is especially promising for the analysis of tissues or cells resistant to transfection, of which the overexpression of tagged ubiquitin is a major hurdle. ..
  8. Donaldson K, Yin H, Gekakis N, Supek F, Joazeiro C. Ubiquitin signals protein trafficking via interaction with a novel ubiquitin binding domain in the membrane fusion regulator, Vps9p. Curr Biol. 2003;13:258-62 pubmed
    ..Here we report that Ub controls yet a third step in the Vps pathway. Vps9p, which promotes endosomal and Golgi-derived vesicle fusion, binds directly to Ub via a Cue1p-homologous (CUE) ..
  9. Nickerson D, Russell M, Lo S, Chapin H, Milnes J, Merz A. Termination of isoform-selective Vps21/Rab5 signaling at endolysosomal organelles by Msb3/Gyp3. Traffic. 2012;13:1411-1428 pubmed publisher
    ..Gyp3, therefore, operates as a compartmental insulator that helps to define the spatial domain of Vps21 signaling in the endolysosomal pathway. ..
  10. Lo S, Brett C, Plemel R, Vignali M, Fields S, Gonen T, et al. Intrinsic tethering activity of endosomal Rab proteins. Nat Struct Mol Biol. 2011;19:40-7 pubmed publisher
    ..In our working model, the intrinsic tethering capacity Vps21 operates in concert with conventional effectors and SNAREs to drive efficient docking and fusion. ..
  11. Cabrera M, Ungermann C. Guanine nucleotide exchange factors (GEFs) have a critical but not exclusive role in organelle localization of Rab GTPases. J Biol Chem. 2013;288:28704-12 pubmed publisher
    ..Our data agree with the emerging model that GEFs are critical for Rab localization but raise the possibility that additional factors can contribute to this process...
  12. Shiflett S, Vaughn M, Huynh D, Kaplan J, Ward D. Bph1p, the Saccharomyces cerevisiae homologue of CHS1/beige, functions in cell wall formation and protein sorting. Traffic. 2004;5:700-10 pubmed
    ..Together, these data suggest that Bph1p associates with a membrane and is involved in protein sorting and cell wall formation. ..
  13. Bean B, Davey M, Snider J, Jessulat M, Deineko V, Tinney M, et al. Rab5-family guanine nucleotide exchange factors bind retromer and promote its recruitment to endosomes. Mol Biol Cell. 2015;26:1119-28 pubmed publisher
    ..The interaction of retromer with distinct VPS9 GEFs could thus link GEF-dependent regulatory inputs to the temporal or spatial coordination of retromer assembly or function. ..