Gene Symbol: VPS54
Description: Vps54p
Alias: CGP1, LUV1, TCS3, Vps54p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Conibear E, Cleck J, Stevens T. Vps51p mediates the association of the GARP (Vps52/53/54) complex with the late Golgi t-SNARE Tlg1p. Mol Biol Cell. 2003;14:1610-23 pubmed
    ..The binding of this small, coiled-coil protein to the conserved N-terminal domain of the t-SNARE therefore provides a crucial link between components of the tethering and the fusion machinery. ..
  2. Siniossoglou S, Pelham H. An effector of Ypt6p binds the SNARE Tlg1p and mediates selective fusion of vesicles with late Golgi membranes. EMBO J. 2001;20:5991-8 pubmed
    ..We suggest that the VFT complex links vesicles containing Tlg1p to their target, which is defined by the local activation of Ypt6p. ..
  3. Bensen E, Costaguta G, Payne G. Synthetic genetic interactions with temperature-sensitive clathrin in Saccharomyces cerevisiae. Roles for synaptojanin-like Inp53p and dynamin-related Vps1p in clathrin-dependent protein sorting at the trans-Golgi network. Genetics. 2000;154:83-97 pubmed
    ..mutations in the following: RIC1, encoding a product originally proposed to participate in ribosome biogenesis; LUV1, encoding a product potentially involved in vacuole and microtubule organization; and INP53, encoding a ..
  4. Fröhlich F, Petit C, Kory N, Christiano R, Hannibal Bach H, Graham M, et al. The GARP complex is required for cellular sphingolipid homeostasis. elife. 2015;4: pubmed publisher
    ..Together, these data show that GARP is essential for cellular sphingolipid homeostasis and suggest a therapeutic strategy for the treatment of PCCA2. ..
  5. Morishita M, Mendonsa R, Wright J, Engebrecht J. Snc1p v-SNARE transport to the prospore membrane during yeast sporulation is dependent on endosomal retrieval pathways. Traffic. 2007;8:1231-45 pubmed
    ..Taken together, these results indicate that retrograde trafficking from the endosome is essential for sporulation by retrieving molecules important for PSM and spore wall formation. ..
  6. Siniossoglou S. Affinity purification of Ypt6 effectors and identification of TMF/ARA160 as a Rab6 interactor. Methods Enzymol. 2005;403:599-607 pubmed
    ..The mammalian homolog of Sgm1, TMF/ARA160, contains a short conserved coiled-coil motif that is sufficient for the binding to the three mammalian orthologs of Ypt6, Rab6A, Rab6A', and Rab6B. ..
  7. Quenneville N, Chao T, McCaffery J, Conibear E. Domains within the GARP subunit Vps54 confer separate functions in complex assembly and early endosome recognition. Mol Biol Cell. 2006;17:1859-70 pubmed
    ..We have found that a subunit of the GARP complex, Vps54, can be separated into N- and C-terminal regions that have different functions...
  8. Bryant N, James D. Vps45p stabilizes the syntaxin homologue Tlg2p and positively regulates SNARE complex formation. EMBO J. 2001;20:3380-8 pubmed
    ..First, SM proteins act as chaperone-like molecules for their cognate t-SNAREs. Secondly, SM proteins play an essential role in the activation process allowing their cognate t-SNARE to participate in ternary complex formation. ..
  9. Conibear E, Stevens T. Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi. Mol Biol Cell. 2000;11:305-23 pubmed
    ..Native coimmunoprecipitation experiments indicate that Vps52p, Vps53p, and Vps54p are associated in a 1:1:1 complex that sediments as a single peak on sucrose velocity gradients...

More Information


  1. Smith A, Archer J, Solomon F. Regulation of tubulin polypeptides and microtubule function: Luv1p [correction of Rki1p] interacts with the beta-tubulin binding protein Rbl2p. Chromosoma. 1998;107:471-8 pubmed
    ..Here we describe the identification of a novel yeast gene, LUV1 [corrected], that interacts genetically with RBL2...
  2. Fiedler T, Karpova T, Fleig U, Young M, Cooper J, Hegemann J. The vesicular transport protein Cgp1p/Vps54p/Tcs3p/Luv1p is required for the integrity of the actin cytoskeleton. Mol Genet Genomics. 2002;268:190-205 pubmed
    ..Cgp1p was recently identified by several groups as Vps54p, which is a member of the VFT complex that is involved in vesicular protein transport at the level of the late ..
  3. Reggiori F, Wang C, Stromhaug P, Shintani T, Klionsky D. Vps51 is part of the yeast Vps fifty-three tethering complex essential for retrograde traffic from the early endosome and Cvt vesicle completion. J Biol Chem. 2003;278:5009-20 pubmed
    ..We demonstrate that Ykr020 is a subunit of the Vps fifty-three tethering complex, composed of Vps52, Vps53, and Vps54, which is required for retrograde traffic from the early endosome back to the late Golgi, and for this reason we ..
  4. Panic B, Whyte J, Munro S. The ARF-like GTPases Arl1p and Arl3p act in a pathway that interacts with vesicle-tethering factors at the Golgi apparatus. Curr Biol. 2003;13:405-10 pubmed
    ..Finally, we find that Arl3p is required in vivo for the targeting of Arl1p, explaining its requirement for the normal distribution of Imh1p. ..
  5. Siniossoglou S, Pelham H. Vps51p links the VFT complex to the SNARE Tlg1p. J Biol Chem. 2002;277:48318-24 pubmed
    ..of the SNARE Snc1p to the plasma membrane, but still assemble a core VFT complex consisting of Vps52p, Vps53p, and Vps54p that localizes properly to the Golgi. Binding to Ypt6-GTP is a property of Vps52p...
  6. Kishimoto T, Yamamoto T, Tanaka K. Defects in structural integrity of ergosterol and the Cdc50p-Drs2p putative phospholipid translocase cause accumulation of endocytic membranes, onto which actin patches are assembled in yeast. Mol Biol Cell. 2005;16:5592-609 pubmed
    ..Simultaneous loss of both phospholipid asymmetry and sterol structural integrity could lead to accumulation of endocytic intermediates capable of initiating assembly of actin patches in the cytoplasm. ..
  7. Dulubova I, Yamaguchi T, Gao Y, Min S, Huryeva I, Sudhof T, et al. How Tlg2p/syntaxin 16 'snares' Vps45. EMBO J. 2002;21:3620-31 pubmed
    ..Thus, this mechanism represents the most widespread mode of coupling between syntaxins and SM proteins. ..
  8. Guo J, Polymenis M. Dcr2 targets Ire1 and downregulates the unfolded protein response in Saccharomyces cerevisiae. EMBO Rep. 2006;7:1124-7 pubmed
    ..Our results are consistent with de-phosphorylation of Ire1 being a mechanism for antagonizing UPR signalling. ..
  9. Yamamoto T, Mochida J, Kadota J, Takeda M, Bi E, Tanaka K. Initial polarized bud growth by endocytic recycling in the absence of actin cable-dependent vesicle transport in yeast. Mol Biol Cell. 2010;21:1237-52 pubmed publisher
  10. Rojas Benítez D, Thiaville P, de Crécy Lagard V, Glavic A. The Levels of a Universally Conserved tRNA Modification Regulate Cell Growth. J Biol Chem. 2015;290:18699-707 pubmed publisher
    ..In the present work, we show that loss of the Drosophila tcs3 homolog also leads to a severe reduction in size and demonstrate, for the first time in a non-microbe, that Tcs3 is ..
  11. Chou H, Dukovski D, Chambers M, Reinisch K, Walz T. CATCHR, HOPS and CORVET tethering complexes share a similar architecture. Nat Struct Mol Biol. 2016;23:761-3 pubmed publisher
    ..We also show that HOPS, a tethering complex acting in the endolysosomal pathway, shares a similar architecture, thus suggesting that multisubunit tethering complexes use related structural frameworks. ..