Gene Symbol: VPS53
Description: Vps53p
Alias: Vps53p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Siniossoglou S, Pelham H. An effector of Ypt6p binds the SNARE Tlg1p and mediates selective fusion of vesicles with late Golgi membranes. EMBO J. 2001;20:5991-8 pubmed
    ..We suggest that the VFT complex links vesicles containing Tlg1p to their target, which is defined by the local activation of Ypt6p. ..
  2. Siniossoglou S, Pelham H. Vps51p links the VFT complex to the SNARE Tlg1p. J Biol Chem. 2002;277:48318-24 pubmed
    ..recycling of the SNARE Snc1p to the plasma membrane, but still assemble a core VFT complex consisting of Vps52p, Vps53p, and Vps54p that localizes properly to the Golgi. Binding to Ypt6-GTP is a property of Vps52p...
  3. Reggiori F, Wang C, Stromhaug P, Shintani T, Klionsky D. Vps51 is part of the yeast Vps fifty-three tethering complex essential for retrograde traffic from the early endosome and Cvt vesicle completion. J Biol Chem. 2003;278:5009-20 pubmed
    ..We demonstrate that Ykr020 is a subunit of the Vps fifty-three tethering complex, composed of Vps52, Vps53, and Vps54, which is required for retrograde traffic from the early endosome back to the late Golgi, and for this ..
  4. VASAN N, Hutagalung A, Novick P, Reinisch K. Structure of a C-terminal fragment of its Vps53 subunit suggests similarity of Golgi-associated retrograde protein (GARP) complex to a family of tethering complexes. Proc Natl Acad Sci U S A. 2010;107:14176-81 pubmed publisher
    ..Here we present the structure of a C-terminal fragment of the Vps53 subunit, important for binding endosome-derived vesicles, at a resolution of 2.9 A...
  5. Feinstein M, Flusser H, Lerman Sagie T, Ben Zeev B, Lev D, Agamy O, et al. VPS53 mutations cause progressive cerebello-cerebral atrophy type 2 (PCCA2). J Med Genet. 2014;51:303-8 pubmed publisher
    ..two mutations within this locus, which were common to the affected individuals: compound heterozygous mutations in VPS53, segregating as expected for autosomal recessive heredity within all four families, and common in Moroccan Jews (?..
  6. Conibear E, Stevens T. Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi. Mol Biol Cell. 2000;11:305-23 pubmed
    ..Native coimmunoprecipitation experiments indicate that Vps52p, Vps53p, and Vps54p are associated in a 1:1:1 complex that sediments as a single peak on sucrose velocity gradients...
  7. Lee C, Pu H, Wang L, Sayler R, Yeh C, Wu S. Mutation in a homolog of yeast Vps53p accounts for the heat and osmotic hypersensitive phenotypes in Arabidopsis hit1-1 mutant. Planta. 2006;224:330-8 pubmed
    ..the HIT1 gene encodes a protein of 829 amino acid residues and is homologous to yeast (Saccharomyces cerevisiae) Vps53p protein...
  8. Siniossoglou S. Affinity purification of Ypt6 effectors and identification of TMF/ARA160 as a Rab6 interactor. Methods Enzymol. 2005;403:599-607 pubmed
    ..The mammalian homolog of Sgm1, TMF/ARA160, contains a short conserved coiled-coil motif that is sufficient for the binding to the three mammalian orthologs of Ypt6, Rab6A, Rab6A', and Rab6B. ..
  9. Chou H, Dukovski D, Chambers M, Reinisch K, Walz T. CATCHR, HOPS and CORVET tethering complexes share a similar architecture. Nat Struct Mol Biol. 2016;23:761-3 pubmed publisher
    ..We also show that HOPS, a tethering complex acting in the endolysosomal pathway, shares a similar architecture, thus suggesting that multisubunit tethering complexes use related structural frameworks. ..

More Information


  1. VanRheenen S, Reilly B, Chamberlain S, Waters M. Dsl1p, an essential protein required for membrane traffic at the endoplasmic reticulum/Golgi interface in yeast. Traffic. 2001;2:212-31 pubmed
  2. Panic B, Whyte J, Munro S. The ARF-like GTPases Arl1p and Arl3p act in a pathway that interacts with vesicle-tethering factors at the Golgi apparatus. Curr Biol. 2003;13:405-10 pubmed
    ..Finally, we find that Arl3p is required in vivo for the targeting of Arl1p, explaining its requirement for the normal distribution of Imh1p. ..
  3. Conibear E, Cleck J, Stevens T. Vps51p mediates the association of the GARP (Vps52/53/54) complex with the late Golgi t-SNARE Tlg1p. Mol Biol Cell. 2003;14:1610-23 pubmed
    ..The binding of this small, coiled-coil protein to the conserved N-terminal domain of the t-SNARE therefore provides a crucial link between components of the tethering and the fusion machinery. ..
  4. Reggiori F, Klionsky D. Atg9 sorting from mitochondria is impaired in early secretion and VFT-complex mutants in Saccharomyces cerevisiae. J Cell Sci. 2006;119:2903-11 pubmed
    ..All mutants have been previously shown to be required for the normal progression of both the Cvt pathway and autophagy, but their precise role in these transport routes was unknown. ..
  5. He X, Qian W, Wang Z, Li Y, Zhang J. Prevalent positive epistasis in Escherichia coli and Saccharomyces cerevisiae metabolic networks. Nat Genet. 2010;42:272-6 pubmed publisher
    ..We offer mechanistic explanations of these findings and experimentally validate them for 61 S. cerevisiae gene pairs. ..
  6. Fröhlich F, Petit C, Kory N, Christiano R, Hannibal Bach H, Graham M, et al. The GARP complex is required for cellular sphingolipid homeostasis. elife. 2015;4: pubmed publisher
    ..A GARP complex mutation analogous to a VPS53 allele causing progressive cerebello-cerebral atrophy type 2 (PCCA2) in humans exhibits similar, albeit weaker, ..
  7. Quenneville N, Chao T, McCaffery J, Conibear E. Domains within the GARP subunit Vps54 confer separate functions in complex assembly and early endosome recognition. Mol Biol Cell. 2006;17:1859-70 pubmed
    ..These data suggest that separate domains recruit tethering complexes to different upstream compartments to regulate individual trafficking pathways. ..