Gene Symbol: VPS52
Description: Vps52p
Alias: SAC2, Vps52p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Kölling R, Lee A, Chen E, Botstein D. Nucleotide sequence of the SAC2 gene of Saccharomyces cerevisiae. Yeast. 1994;10:1211-6 pubmed
    ..mutation (act1-1) in the essential actin gene of Saccharomyces cerevisiae can be suppressed by mutations in the SAC2 gene...
  2. Siniossoglou S, Pelham H. An effector of Ypt6p binds the SNARE Tlg1p and mediates selective fusion of vesicles with late Golgi membranes. EMBO J. 2001;20:5991-8 pubmed
    ..We show here that a conserved trimeric protein complex, VFT (Vps52/53/54), binds directly to Ypt6p:GTP...
  3. Conibear E, Stevens T. Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi. Mol Biol Cell. 2000;11:305-23 pubmed
    ..Native coimmunoprecipitation experiments indicate that Vps52p, Vps53p, and Vps54p are associated in a 1:1:1 complex that sediments as a single peak on sucrose velocity ..
  4. Fiedler T, Karpova T, Fleig U, Young M, Cooper J, Hegemann J. The vesicular transport protein Cgp1p/Vps54p/Tcs3p/Luv1p is required for the integrity of the actin cytoskeleton. Mol Genet Genomics. 2002;268:190-205 pubmed
    ..Our data show for the first time that Cgp1p/Vps54p links aspects of vesicular protein transport with the organization of the actin cytoskeleton. ..
  5. Reggiori F, Wang C, Stromhaug P, Shintani T, Klionsky D. Vps51 is part of the yeast Vps fifty-three tethering complex essential for retrograde traffic from the early endosome and Cvt vesicle completion. J Biol Chem. 2003;278:5009-20 pubmed
    ..We demonstrate that Ykr020 is a subunit of the Vps fifty-three tethering complex, composed of Vps52, Vps53, and Vps54, which is required for retrograde traffic from the early endosome back to the late Golgi, and ..
  6. Novick P, Osmond B, Botstein D. Suppressors of yeast actin mutations. Genetics. 1989;121:659-74 pubmed
    ..Three of the genes (SAC1, SAC2 and SAC3) were subjected to extensive genetic and phenotypic analysis, including molecular cloning...
  7. Siniossoglou S, Pelham H. Vps51p links the VFT complex to the SNARE Tlg1p. J Biol Chem. 2002;277:48318-24 pubmed
    ..and recycling of the SNARE Snc1p to the plasma membrane, but still assemble a core VFT complex consisting of Vps52p, Vps53p, and Vps54p that localizes properly to the Golgi. Binding to Ypt6-GTP is a property of Vps52p...
  8. Conibear E, Cleck J, Stevens T. Vps51p mediates the association of the GARP (Vps52/53/54) complex with the late Golgi t-SNARE Tlg1p. Mol Biol Cell. 2003;14:1610-23 pubmed
    ..We have previously identified three subunits of a Golgi-localized complex (the Vps52/53/54 complex) that is required for retrograde transport to the late Golgi...
  9. Siniossoglou S. Affinity purification of Ypt6 effectors and identification of TMF/ARA160 as a Rab6 interactor. Methods Enzymol. 2005;403:599-607 pubmed
    ..The mammalian homolog of Sgm1, TMF/ARA160, contains a short conserved coiled-coil motif that is sufficient for the binding to the three mammalian orthologs of Ypt6, Rab6A, Rab6A', and Rab6B. ..

More Information


  1. Soper J, Kehm V, Burd C, Bankaitis V, Lee V. Aggregation of ?-synuclein in S. cerevisiae is associated with defects in endosomal trafficking and phospholipid biosynthesis. J Mol Neurosci. 2011;43:391-405 pubmed publisher
    ..These data suggest new pathogenic mechanisms for ?-synuclein neurotoxicity. ..
  2. Webster M, McCaffery J, COHEN FIX O. Vesicle trafficking maintains nuclear shape in Saccharomyces cerevisiae during membrane proliferation. J Cell Biol. 2010;191:1079-88 pubmed publisher
    ..Moreover, flares may facilitate maintenance of a constant nuclear/cell volume ratio in the face of altered membrane proliferation. ..
  3. Fröhlich F, Petit C, Kory N, Christiano R, Hannibal Bach H, Graham M, et al. The GARP complex is required for cellular sphingolipid homeostasis. elife. 2015;4: pubmed publisher
    ..Together, these data show that GARP is essential for cellular sphingolipid homeostasis and suggest a therapeutic strategy for the treatment of PCCA2. ..
  4. Chou H, Dukovski D, Chambers M, Reinisch K, Walz T. CATCHR, HOPS and CORVET tethering complexes share a similar architecture. Nat Struct Mol Biol. 2016;23:761-3 pubmed publisher
    ..We also show that HOPS, a tethering complex acting in the endolysosomal pathway, shares a similar architecture, thus suggesting that multisubunit tethering complexes use related structural frameworks. ..
  5. Winsor B, Schiebel E. Review: an overview of the Saccharomyces cerevisiae microtubule and microfilament cytoskeleton. Yeast. 1997;13:399-434 pubmed
  6. Kishimoto T, Yamamoto T, Tanaka K. Defects in structural integrity of ergosterol and the Cdc50p-Drs2p putative phospholipid translocase cause accumulation of endocytic membranes, onto which actin patches are assembled in yeast. Mol Biol Cell. 2005;16:5592-609 pubmed
    ..Simultaneous loss of both phospholipid asymmetry and sterol structural integrity could lead to accumulation of endocytic intermediates capable of initiating assembly of actin patches in the cytoplasm. ..
  7. Quenneville N, Chao T, McCaffery J, Conibear E. Domains within the GARP subunit Vps54 confer separate functions in complex assembly and early endosome recognition. Mol Biol Cell. 2006;17:1859-70 pubmed
    ..These data suggest that separate domains recruit tethering complexes to different upstream compartments to regulate individual trafficking pathways. ..