VPS5

Summary

Gene Symbol: VPS5
Description: sorting nexin 1
Alias: GRD2, PEP10, VPT5, sorting nexin 1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Horazdovsky B, Davies B, Seaman M, McLaughlin S, Yoon S, Emr S. A sorting nexin-1 homologue, Vps5p, forms a complex with Vps17p and is required for recycling the vacuolar protein-sorting receptor. Mol Biol Cell. 1997;8:1529-41 pubmed
    ..Unlike wild-type cells that contain 1-3 large vacuolar structures, the class B vps5 and vps17 mutant cells contain 10-20 smaller vacuole-like compartments...
  2. Seaman M, McCaffery J, Emr S. A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast. J Cell Biol. 1998;142:665-81 pubmed
    ..Conservation of the yeast retromer complex components in higher eukaryotes suggests an important general role for this complex in endosome-to-Golgi retrieval. ..
  3. Bensen E, Costaguta G, Payne G. Synthetic genetic interactions with temperature-sensitive clathrin in Saccharomyces cerevisiae. Roles for synaptojanin-like Inp53p and dynamin-related Vps1p in clathrin-dependent protein sorting at the trans-Golgi network. Genetics. 2000;154:83-97 pubmed
    ..Our findings implicate a wide variety of proteins in clathrin-dependent processes and provide evidence for the selective involvement of Inp53p in clathrin-mediated protein sorting at the TGN. ..
  4. Nothwehr S, Bryant N, Stevens T. The newly identified yeast GRD genes are required for retention of late-Golgi membrane proteins. Mol Cell Biol. 1996;16:2700-7 pubmed
    ..All of the grd mutants exhibited significant mislocalization of A-ALP, as measured by determining the kinetics of A-ALP processing and by analyzing its ..
  5. Nothwehr S, Hindes A. The yeast VPS5/GRD2 gene encodes a sorting nexin-1-like protein required for localizing membrane proteins to the late Golgi. J Cell Sci. 1997;110 ( Pt 9):1063-72 pubmed
    ..Here we describe one of the GRD genes, VPSS/GRD2, that encodes a hydrophilic protein similar to human sorting nexin-1, a protein involved in trafficking of the ..
  6. Reddy J, Seaman M. Vps26p, a component of retromer, directs the interactions of Vps35p in endosome-to-Golgi retrieval. Mol Biol Cell. 2001;12:3242-56 pubmed
    ..These domain swap experiments have shown that Vps26p promotes the interactions between the cargo-selective component Vps35p and the structural components Vps5p/Vps17p. ..
  7. Seaman M, Williams H. Identification of the functional domains of yeast sorting nexins Vps5p and Vps17p. Mol Biol Cell. 2002;13:2826-40 pubmed
    ..Additionally, we have examined which domain(s) of Vps5p is necessary for membrane association. ..
  8. Yu J, Lemmon M. All phox homology (PX) domains from Saccharomyces cerevisiae specifically recognize phosphatidylinositol 3-phosphate. J Biol Chem. 2001;276:44179-84 pubmed
    ..Our results establish that PtdIns-3-P, and not other phosphoinositides, is the target of all PX domains in S. cerevisiae and suggest a role for PX domains in assembly of multiprotein complexes at specific membrane surfaces. ..
  9. Gokool S, Tattersall D, Reddy J, Seaman M. Identification of a conserved motif required for Vps35p/Vps26p interaction and assembly of the retromer complex. Biochem J. 2007;408:287-95 pubmed
    ..These data implicate Vps26p/VPS26 in regulating Vps35p/VPS35 membrane association and therefore suggest a role for Vps26p/VPS26 in cargo recognition. ..

More Information

Publications12

  1. Mattiazzi M, Jambhekar A, Kaferle P, DeRisi J, Krizaj I, Petrovic U. Genetic interactions between a phospholipase A2 and the Rim101 pathway components in S. cerevisiae reveal a role for this pathway in response to changes in membrane composition and shape. Mol Genet Genomics. 2010;283:519-30 pubmed publisher
  2. Restrepo R, Zhao X, Peter H, Zhang B, Arvan P, Nothwehr S. Structural features of vps35p involved in interaction with other subunits of the retromer complex. Traffic. 2007;8:1841-53 pubmed
  3. Bean B, Davey M, Snider J, Jessulat M, Deineko V, Tinney M, et al. Rab5-family guanine nucleotide exchange factors bind retromer and promote its recruitment to endosomes. Mol Biol Cell. 2015;26:1119-28 pubmed publisher
    ..The interaction of retromer with distinct VPS9 GEFs could thus link GEF-dependent regulatory inputs to the temporal or spatial coordination of retromer assembly or function. ..