VPS4

Summary

Gene Symbol: VPS4
Description: AAA family ATPase VPS4
Alias: CSC1, DID6, END13, GRD13, VPL4, VPT10
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. pmc Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta1
    Ishara Azmi
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    J Cell Biol 172:705-17. 2006
  2. pmc Biochemical and structural studies of yeast Vps4 oligomerization
    Malgorzata D Gonciarz
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84112 5650, USA
    J Mol Biol 384:878-95. 2008
  3. pmc Cryo-EM structure of dodecameric Vps4p and its 2:1 complex with Vta1p
    Zhiheng Yu
    Division of Biology, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA
    J Mol Biol 377:364-77. 2008
  4. doi Vacuolar protein sorting: two different functional states of the AAA-ATPase Vps4p
    Claudia Hartmann
    Institute of Biochemistry, University of Zurich, Winterthurer Strasse 190, 8057 Zurich, Switzerland
    J Mol Biol 377:352-63. 2008
  5. doi The Vps4 C-terminal helix is a critical determinant for assembly and ATPase activity and has elements conserved in other members of the meiotic clade of AAA ATPases
    Parimala R Vajjhala
    Institute for Molecular Bioscience, The University of Queensland, Australia
    FEBS J 275:1427-49. 2008
  6. doi ESCRT-III family members stimulate Vps4 ATPase activity directly or via Vta1
    Ishara F Azmi
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Dev Cell 14:50-61. 2008
  7. pmc Structural basis of Vta1 function in the multivesicular body sorting pathway
    Junyu Xiao
    Life Sciences Institute and Department of Biological Chemistry, Medical School, University of Michigan, Ann Arbor, MI 48109, USA
    Dev Cell 14:37-49. 2008
  8. pmc Novel Ist1-Did2 complex functions at a late step in multivesicular body sorting
    Sarah M Rue
    Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA
    Mol Biol Cell 19:475-84. 2008
  9. pmc Ist1 regulates Vps4 localization and assembly
    Christian Dimaano
    Department of Biology, University of Utah, Salt Lake City, UT 84112 9202, USA
    Mol Biol Cell 19:465-74. 2008
  10. pmc Membrane scission by the ESCRT-III complex
    Thomas Wollert
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, US Department of Health and Human Services, Bethesda, Maryland 20892, USA
    Nature 458:172-7. 2009

Scientific Experts

  • Laran T Jensen
  • Marta Hoffman-Sommer
  • David J Katzmann
  • Brian A Davies
  • Daniel P Nickerson
  • Markus Babst
  • Parimala R Vajjhala
  • Zhaohui Xu
  • Junyu Xiao
  • Anna Shestakova
  • James H Hurley
  • Greg Odorizzi
  • Matt Curtiss
  • Alan L Munn
  • Malgorzata D Gonciarz
  • Ishara F Azmi
  • Christopher P Hill
  • Wesley I Sundquist
  • Tess Shideler
  • Charles B Jones
  • Frank G Whitby
  • Christian Dimaano
  • Bei Yang
  • Jason Tan
  • Scott D Emr
  • Brant M Webster
  • Johanna A Payne
  • Manuel Alonso Y Adell
  • Cody J Vild
  • Nicole Monroe
  • Andrew P Norgan
  • Barbara Sciskala
  • Matthew R G Russell
  • Ralf Kölling
  • Xuefeng Ren
  • Debra M Eckert
  • Usha Nair
  • Henning Arlt
  • Dong Yang
  • Abraham Hanono
  • Thomas Wollert
  • Peter Weiss
  • Matthew West
  • Michio Inoue
  • Zhiheng Yu
  • Fan Zhang
  • Claudia Hartmann
  • Sarah M Rue
  • Chau H Nguyen
  • Suraj Saksena
  • Carol Kistler
  • Jiahai Zhou
  • Hengchuan Xia
  • Takayuki Obita
  • Ruth Nebauer
  • Jillian M Lottridge
  • Ishara Azmi
  • Penghua Wang
  • Vicki A Sciorra
  • Anna Scott
  • Michio Hayashi
  • Nario Tomishige
  • Tom H Stevens
  • M Babst
  • Katherine Bowers
  • Paola Fiorani
  • Lorena Eguez
  • Hongyuan Yang
  • Sebastian C L Yeo
  • Qingtao Shen
  • Goran Stjepanovic
  • Alexey J Merz
  • Linda M Benson
  • Andreas Martin
  • Michael W Hess
  • Herbert Lindner
  • David Teis
  • Edina Harsay
  • Mehrshad Pakdel
  • Georg F Vogel
  • Jens Jager
  • Han Han
  • Martin Muller
  • Paolo Colombi
  • Mary Anne Karren
  • C Patrick Lusk
  • Andreas S Schroeder
  • Gregory M Lynch
  • Justin M Keener
  • Elizabeth M Ott

Detail Information

Publications56

  1. pmc Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta1
    Ishara Azmi
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    J Cell Biol 172:705-17. 2006
    ..b>Vps4, an ATPase associated with a variety of cellular activities, is required late in the MVB sorting reaction to ..
  2. pmc Biochemical and structural studies of yeast Vps4 oligomerization
    Malgorzata D Gonciarz
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84112 5650, USA
    J Mol Biol 384:878-95. 2008
    ..As the only known enzyme in the ESCRT pathway, the AAA ATPase (ATPase associated with diverse cellular activities) Vps4 provides the energy required for multiple rounds of vesicle formation...
  3. pmc Cryo-EM structure of dodecameric Vps4p and its 2:1 complex with Vta1p
    Zhiheng Yu
    Division of Biology, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA
    J Mol Biol 377:364-77. 2008
    The type I AAA (ATPase associated with a variety of cellular activities) ATPase Vps4 and its co-factor Vta1p/LIP5 function in membrane remodeling events that accompany cytokinesis, multivesicular body biogenesis, and retrovirus budding, ..
  4. doi Vacuolar protein sorting: two different functional states of the AAA-ATPase Vps4p
    Claudia Hartmann
    Institute of Biochemistry, University of Zurich, Winterthurer Strasse 190, 8057 Zurich, Switzerland
    J Mol Biol 377:352-63. 2008
    The vacuolar protein sorting (Vps) pathway, in which Vps4 class I AAA-ATPases play a central role, regulates growth factor receptors, immune response, and developmental signaling, and participates in tumor suppression, apoptosis, and ..
  5. doi The Vps4 C-terminal helix is a critical determinant for assembly and ATPase activity and has elements conserved in other members of the meiotic clade of AAA ATPases
    Parimala R Vajjhala
    Institute for Molecular Bioscience, The University of Queensland, Australia
    FEBS J 275:1427-49. 2008
    ..b>Vps4 is an AAA ATPase that functions in MVB sorting...
  6. doi ESCRT-III family members stimulate Vps4 ATPase activity directly or via Vta1
    Ishara F Azmi
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Dev Cell 14:50-61. 2008
    The AAA-ATPase Vps4 is critical for function of the MVB sorting pathway, which in turn impacts cellular phenomena ranging from receptor downregulation to viral budding to cytokinesis...
  7. pmc Structural basis of Vta1 function in the multivesicular body sorting pathway
    Junyu Xiao
    Life Sciences Institute and Department of Biological Chemistry, Medical School, University of Michigan, Ann Arbor, MI 48109, USA
    Dev Cell 14:37-49. 2008
    ..Proper function of the MVB pathway requires reversible membrane association of the ESCRTs, a process catalyzed by Vps4 ATPase. Vta1 regulates the Vps4 activity, but its mechanism of action was poorly understood...
  8. pmc Novel Ist1-Did2 complex functions at a late step in multivesicular body sorting
    Sarah M Rue
    Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093, USA
    Mol Biol Cell 19:475-84. 2008
    ..Ist1-Did2 and Vta1-Vps60 form specific physical complexes, and, like Did2 and Vta1, Ist1 binds to the AAA-ATPase Vps4. We provide evidence that the ist1Delta mutation exhibits a synthetic interaction with mutations in VPS2 (DID4) ..
  9. pmc Ist1 regulates Vps4 localization and assembly
    Christian Dimaano
    Department of Biology, University of Utah, Salt Lake City, UT 84112 9202, USA
    Mol Biol Cell 19:465-74. 2008
    ..The dissociation of ESCRT machinery by the AAA-type ATPase Vps4 is a necessary late step in the formation of MVB vesicles...
  10. pmc Membrane scission by the ESCRT-III complex
    Thomas Wollert
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, US Department of Health and Human Services, Bethesda, Maryland 20892, USA
    Nature 458:172-7. 2009
    ..consists of Vps20, Snf7, Vps24 and Vps2 (also known as Did4), which assemble in that order and require the ATPase Vps4 for their disassembly...
  11. ncbi Structural basis for selective recognition of ESCRT-III by the AAA ATPase Vps4
    Takayuki Obita
    MRC Laboratory of Molecular Biology, Medical Research Council Centre, Cambridge CB2 0QH, UK
    Nature 449:735-9. 2007
    ..The AAA ATPase Vps4, which is central to endosomal traffic to lysosomes, retroviral budding and cytokinesis, dissociates ESCRT ..
  12. pmc Regulators of Vps4 ATPase activity at endosomes differentially influence the size and rate of formation of intralumenal vesicles
    Daniel P Nickerson
    Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309 0347, USA
    Mol Biol Cell 21:1023-32. 2010
    ..Disassembly of the ESCRT-III polymer and dissociation of its subunits from endosomes requires the Vps4 ATPase, the activity of which is controlled in vivo by regulatory proteins...
  13. pmc Assembly of the AAA ATPase Vps4 on ESCRT-III
    Anna Shestakova
    Department of Biology, University of Utah, Salt Lake City, UT 84112 9202, USA
    Mol Biol Cell 21:1059-71. 2010
    b>Vps4 is a key enzyme that functions in endosomal protein trafficking, cytokinesis, and retroviral budding. Vps4 activity is regulated by its recruitment from the cytoplasm to ESCRT-III, where the protein oligomerizes into an active ATPase...
  14. pmc Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes
    Daniel P Nickerson
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    J Cell Biol 175:715-20. 2006
    ..The subsequent dissociation of ESCRT complexes from endosomes requires Vps4, a member of the AAA family of adenosine triphosphatases...
  15. pmc Class E compartments form in response to ESCRT dysfunction in yeast due to hyperactivity of the Vps21 Rab GTPase
    Matthew R G Russell
    Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder, Campus Box 347, Boulder, CO 80309, USA
    J Cell Sci 125:5208-20. 2012
    ..in response to transient ESCRT inactivation and recovery in yeast expressing the temperature-sensitive mutant vps4(ts) allele...
  16. pmc Vta1p and Vps46p regulate the membrane association and ATPase activity of Vps4p at the yeast multivesicular body
    Jillian M Lottridge
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    Proc Natl Acad Sci U S A 103:6202-7. 2006
    ..These results reveal functional roles for the Vps46p and Vta1p proteins in regulating the ESCRT complex assembly/disassembly cycle in protein sorting at the yeast late endosome...
  17. pmc Structural and mechanistic studies of VPS4 proteins
    Anna Scott
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84132 3201, USA
    EMBO J 24:3658-69. 2005
    b>VPS4 ATPases function in multivesicular body formation and in HIV-1 budding...
  18. pmc The linker region plays a regulatory role in assembly and activity of the Vps4 AAA ATPase
    Anna Shestakova
    From the Center for Cell and Genome Science and Department of Biology, University of Utah, Salt Lake City, Utah 84112 and
    J Biol Chem 288:26810-9. 2013
    The AAA-type ATPase Vps4 functions with components of the ESCRT (endosomal sorting complex required for transport) machinery in membrane fission events that are essential for endosomal maturation, cytokinesis, and the formation of ..
  19. pmc The oligomeric state of the active Vps4 AAA ATPase
    Nicole Monroe
    Department of Biochemistry, University of Utah School of Medicine, 15 North Medical Drive East RM 4100, Salt Lake City, UT 84112 5650, USA
    J Mol Biol 426:510-25. 2014
    ..A component of this pathway, the AAA ATPase Vps4, provides energy for pathway progression...
  20. ncbi Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae
    Katherine Bowers
    Cambridge Institute for Medical Research and Department of Clinical Biochemistry, University of Cambridge, Wellcome Trust MRC Building, Addenbrooke s Hospital, Hills Road, Cambridge, CB2 2XY, UK
    Traffic 5:194-210. 2004
    ..We propose the formation of a large multimeric complex on the endosome membrane consisting of ESCRTI, ESCRTII, ESCRTIII and other associated proteins...
  21. ncbi Vps20p and Vta1p interact with Vps4p and function in multivesicular body sorting and endosomal transport in Saccharomyces cerevisiae
    Sebastian C L Yeo
    Institute of Molecular and Cell Biology, The National University of Singapore, Singapore, 117609, Singapore
    J Cell Sci 116:3957-70. 2003
    ..We propose that Vps20p and Vta1p regulate Vps4p function in vivo...
  22. pmc The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways
    A Y Amerik
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Mol Biol Cell 11:3365-80. 2000
    ..However, in cells lacking the Vps4/Did6 ATPase, a large fraction of Doa4-green fluorescent protein, like several other Vps factors, concentrates at ..
  23. pmc The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function
    M Babst
    Division of Cellular Molecular Medicine and Howard Hughes Medical Institute, University of California at San Diego, School of Medicine La Jolla, CA 92093 0668, USA
    EMBO J 17:2982-93. 1998
    ..of two previously uncharacterized class E Vps proteins, Vps24p and Vps32p/Snf7p, was also affected by mutations in VPS4. Upon inactivation of a temperature-conditional vps4 mutant, Vps24p and Vps32p/Snf7p rapidly accumulated in a large ..
  24. pmc Endosomal transport function in yeast requires a novel AAA-type ATPase, Vps4p
    M Babst
    Division of Cellular and Molecular Medicine and Howard Hughes Medical Institute, University of California at San Diego, School of Medicine, La Jolla 92093 0668, USA comment
    EMBO J 16:1820-31. 1997
    ..The vacuolar protein sorting (vps) mutant vps4 accumulates vacuolar, endocytic and late-Golgi markers in an aberrant multilamellar pre-vacuolar compartment...
  25. pmc Conformational Changes in the Endosomal Sorting Complex Required for the Transport III Subunit Ist1 Lead to Distinct Modes of ATPase Vps4 Regulation
    Jason Tan
    From the Biochemistry and Molecular Biology Department, Mayo Graduate School, and
    J Biol Chem 290:30053-65. 2015
    ..The ATPase Vps4 remodels and disassembles ESCRT-III, but the manner in which Vps4 activity is coordinated with ESCRT-III function ..
  26. pmc Relief of autoinhibition enhances Vta1 activation of Vps4 via the Vps4 stimulatory element
    Andrew P Norgan
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    J Biol Chem 288:26147-56. 2013
    ..The AAA-ATPase Vps4 is required for ESCRT function, and its full activity is dependent upon the co-factor Vta1...
  27. pmc Interaction maps of the Saccharomyces cerevisiae ESCRT-III protein Snf7
    Barbara Sciskala
    Institut für Lebensmittelwissenschaft und Biotechnologie, Fg Gärungstechnologie, Universitat Hohenheim, Stuttgart, Germany
    Eukaryot Cell 12:1538-46. 2013
    ..b>Vps4 bound to individual Snf7 motifs but not to full-length Snf7...
  28. pmc Vfa1 binds to the N-terminal microtubule-interacting and trafficking (MIT) domain of Vps4 and stimulates its ATPase activity
    Cody J Vild
    From the Life Sciences Institute and Department of Biological Chemistry, Medical School, University of Michigan, Ann Arbor, Michigan 48109
    J Biol Chem 289:10378-86. 2014
    ..assembled molecular complexes on the membrane, and their disassembly requires the action of the AAA-ATPase Vps4. Vps4 is regulated by a multitude of ESCRT and ESCRT-related proteins...
  29. pmc Coordinated binding of Vps4 to ESCRT-III drives membrane neck constriction during MVB vesicle formation
    Manuel Alonso Y Adell
    Division of Cell Biology and 2 Division of Clinical Biochemistry, Biocenter and 3 Division of Histology and Embryology Innsbruck Medical University, Innsbruck 6020, Austria
    J Cell Biol 205:33-49. 2014
    ..The AAA-adenosine triphosphatase Vps4 disassembles and recycles the ESCRT-III complex, thereby terminating the ESCRT pathway...
  30. pmc Surveillance of nuclear pore complex assembly by ESCRT-III/Vps4
    Brant M Webster
    Department of Cell Biology, Yale School of Medicine, New Haven, CT 06520, USA
    Cell 159:388-401. 2014
    ..Heh2 recruits the endosomal sorting complex required for transport (ESCRT)-III subunit Snf7 and the AAA-ATPase Vps4 to destabilize and clear defective NPC assembly intermediates...
  31. pmc Ubiquitin binding by the CUE domain promotes endosomal localization of the Rab5 GEF Vps9
    Tess Shideler
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309 0347
    Mol Biol Cell 26:1345-56. 2015
    ..These results indicate that ubiquitin binding by the CUE domain promotes Vps9 function in endolysosomal membrane trafficking via promotion of localization...
  32. pmc Vps4 disassembles an ESCRT-III filament by global unfolding and processive translocation
    Bei Yang
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA
    Nat Struct Mol Biol 22:492-8. 2015
    The AAA+ ATPase Vps4 disassembles ESCRT-III and is essential for HIV-1 budding and other pathways. Vps4 is a paradigmatic member of a class of hexameric AAA+ ATPases that disassemble protein complexes without degradation...
  33. ncbi Phosphatidylethanolamine, a limiting factor of autophagy in yeast strains bearing a defect in the carboxypeptidase Y pathway of vacuolar targeting
    Ruth Nebauer
    Institute of Biochemistry, Graz University of Technology, Petersgasse 12 2, A 8010 Graz, Austria
    J Biol Chem 282:16736-43. 2007
    ..We found that deletion of VPS4 and VPS36 caused impaired maturation of the vacuolar proaminopeptidase I (pAPI) via autophagy or the cytosol to ..
  34. pmc Regulation of membrane protein degradation by starvation-response pathways
    Charles B Jones
    Department of Biology, University of Utah, 257 South 1400 East, Salt Lake City, UT 84112 9202, USA
    Traffic 13:468-82. 2012
    ..Together, the data indicate that protein synthesis and turnover are linked by a common regulatory system that ensures adaptation and survival under nutrient-stress conditions...
  35. pmc Endocytosis is required for the growth of vacuolar H(+)-ATPase-defective yeast: identification of six new END genes
    A L Munn
    Department of Biochemistry, Biozentrum of the University of Basel, Switzerland
    J Cell Biol 127:373-86. 1994
    ..and end11-1) show altered uptake of the endocytosed ligand, alpha-factor, and three (end12-1, end12-2, and end13-1) are probably defective in transfer of internalized material to the vacuole...
  36. doi Nucleotide-dependent conformational changes and assembly of the AAA ATPase SKD1/VPS4B
    Michio Inoue
    Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization KEK, Tsukuba, Ibaraki 305 0801, Japan
    Traffic 9:2180-9. 2008
    ..ATP hydrolysis would eliminate this interaction and subsequent nucleotide release causes the domains to rotate, which together lead to the disassembly of the SKD1 oligomer...
  37. pmc The newly identified yeast GRD genes are required for retention of late-Golgi membrane proteins
    S F Nothwehr
    Division of Biological Sciences, University of Missouri, Columbia 65211, USA
    Mol Cell Biol 16:2700-7. 1996
    ..All of the grd mutants exhibited significant mislocalization of A-ALP, as measured by determining the kinetics of A-ALP processing and by analyzing its..
  38. ncbi The VPS4 gene is involved in protein transport out of a yeast pre-vacuolar endosome-like compartment
    M Finken-Eigen
    Biologisch Medizinisches Forschungszentrum, Heinrich Heine Universitat Dusseldorf, Moorenstrasse 5, D 40225 Dusseldorf, Germany
    Curr Genet 31:469-80. 1997
    ..The mutant alleles causing the defects were identified as the members of the VPS4 gene locus, each harbouring single-point mutations leading to amino-acid exchanges at positions 233 (E233Q), 211 (..
  39. pmc A subset of yeast vacuolar protein sorting mutants is blocked in one branch of the exocytic pathway
    Edina Harsay
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    J Cell Biol 156:271-85. 2002
    ..that vacuolar protein sorting mutants that block an endosome-mediated route to the vacuole, including vps1, pep12, vps4, and a temperature-sensitive clathrin mutant, missort cargo normally transported by dense exocytic vesicles, such ..
  40. pmc Yeast Mn2+ transporter, Smf1p, is regulated by ubiquitin-dependent vacuolar protein sorting
    Lorena Eguez
    Department of Microbiology and Molecular Genetics and Graduate School of Biomedical Sciences, University of Medicine and Dentistry New Jersey Medical School, Newark, New Jersey 07103 2714, USA
    Genetics 167:107-17. 2004
    ..Because Smf1p is ubiquitinated in vivo, we propose that Smf1p is targeted to the vacuole for degradation by ubiquitination-dependent protein sorting...
  41. ncbi SKG1, a suppressor gene of synthetic lethality of kex2Deltagas1Delta mutations, encodes a novel membrane protein that affects cell wall composition
    Nario Tomishige
    Department of Biotechnology, University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
    Yeast 22:141-55. 2005
    ..Thus SKG1 encodes a novel protein that affects the cell wall polymer composition in the growing region of the cell...
  42. pmc AAA ATPases regulate membrane association of yeast oxysterol binding proteins and sterol metabolism
    Penghua Wang
    Department of Biochemistry, Faculty of Medicine, National University of Singapore, Singapore, Singapore
    EMBO J 24:2989-99. 2005
    ..Deletion of VPS4 induced a dramatic increase in the membrane-associated pools of Osh6p and Osh7p and also caused a decrease in ..
  43. pmc Constitutive activation of the pH-responsive Rim101 pathway in yeast mutants defective in late steps of the MVB/ESCRT pathway
    Michio Hayashi
    Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Mol Cell Biol 25:9478-90. 2005
    ..Three identified suppressors-did4/vps2, vps24, and vps4-all belonged to "class E" vps mutants, which are commonly defective in multivesicular body sorting...
  44. ncbi The beta domain is required for Vps4p oligomerization into a functionally active ATPase
    Parimala R Vajjhala
    Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, University of Queensland, St Lucia, Queensland, Australia
    FEBS J 273:2357-73. 2006
    ..b>Vps4 is an AAA family ATPase which plays a key role in MVB sorting and facilitates transport through endosomes...
  45. pmc SNARE proteins are required for macroautophagy
    Usha Nair
    Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
    Cell 146:290-302. 2011
    ..Thus, multiple SNARE-mediated fusion events are likely to be involved in autophagosome biogenesis...
  46. doi An overexpression screen in Saccharomyces cerevisiae identifies novel genes that affect endocytic protein trafficking
    Henning Arlt
    Department of Biology Chemistry, Biochemistry Section, University of Osnabruck, Osnabrück 49076, Germany
    Traffic 12:1592-603. 2011
    ..Among the novel proteins, we identify Yer128w as an endosomal protein that interacts with the AAA-ATPase Vps4, and therefore named it Vfa1 (Vps Four-Associated 1)...
  47. pmc Structural basis for endosomal recruitment of ESCRT-I by ESCRT-0 in yeast
    Xuefeng Ren
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
    EMBO J 30:2130-9. 2011
    ..However, this site is non-essential for sorting of the ESCRT cargo Cps1. Taken together, these results show how a conserved motif/domain pair can evolve to use strikingly different binding modes in different organisms...
  48. pmc Structural role of the Vps4-Vta1 interface in ESCRT-III recycling
    Dong Yang
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, U S Department of Health and Human Services, Bethesda, MD 20892, USA
    Structure 18:976-84. 2010
    ..The final step in the ESCRT cycle is the disassembly of the ESCRT-III lattice by the AAA+ ATPase Vps4. Vps4 assembles on its membrane-bound ESCRT-III substrate with its cofactor, Vta1...
  49. ncbi Vps4 regulates a subset of protein interactions at the multivesicular endosome
    Parimala R Vajjhala
    Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, University of Queensland, St Lucia, QLD, Australia
    FEBS J 274:1894-907. 2007
    ..Vacuolar protein sorting 4 (Vps4) is an AAA (ATPase associated with a variety of cellular activities) ATPase which has been proposed to play a ..
  50. doi Analysis of the dual function of the ESCRT-III protein Snf7 in endocytic trafficking and in gene expression
    Peter Weiss
    Institut für Lebensmittelwissenschaft und Biotechnologie, Fg Gärungstechnologie, Universitat Hohenheim, D 70599 Stuttgart, Germany
    Biochem J 424:89-97. 2009
    ..ESCRT-III subunits Vps20 and Vps24 were strongly increased by the mutations, whereas the interactions with proteins Vps4 and Bro1, acting downstream of ESCRT-III, were reduced...
  51. doi Mutations in the Saccharomyces cerevisiae vacuolar fusion proteins Ccz1, Mon1 and Ypt7 cause defects in cell cycle progression in a num1Delta background
    Marta Hoffman-Sommer
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
    Eur J Cell Biol 88:639-52. 2009
    ..Together, these results indicate that deregulation of the cell cycle in these mutants results from two separate mechanisms, one of which is related to calcium homeostasis...
  52. pmc Down-regulation of a manganese transporter in the face of metal toxicity
    Laran T Jensen
    Department of Environmental Health Sciences and Department of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Mol Biol Cell 20:2810-9. 2009
    ..We provide evidence that manganese starvation is sensed within the lumen of the secretory pathway, whereas manganese toxicity is sensed within an extra-Golgi/cytosolic compartment of the cell...
  53. pmc Structural characterization of the ATPase reaction cycle of endosomal AAA protein Vps4
    Junyu Xiao
    Life Sciences Institute and Department of Biological Chemistry, Medical School, University of Michigan, Ann Arbor, MI 48109, USA
    J Mol Biol 374:655-70. 2007
    ..Disassembly of the ESCRTs is catalyzed by ATPase associated with various cellular activities (AAA) protein Vps4. Vps4 contains a single AAA domain and undergoes ATP-dependent quaternary structural change to disassemble the ..
  54. pmc Disrupting vesicular trafficking at the endosome attenuates transcriptional activation by Gcn4
    Fan Zhang
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Biol 28:6796-818. 2008
    ....
  55. pmc Synthetic genetic array analysis of the PtdIns 4-kinase Pik1p identifies components in a Golgi-specific Ypt31/rab-GTPase signaling pathway
    Vicki A Sciorra
    Department of Cellular and Molecular Medicine, Howard Hughes Medical Institute, School of Medicine, University of California at San Diego, La Jolla, CA 92093 0668, USA
    Mol Biol Cell 16:776-93. 2005
    ..We propose that multiple stage-specific signals, which may include Pik1p/PtdIns(4)P, TRAPPII and Gyp2p, impinge upon Ypt31 signaling to regulate Golgi secretory function...
  56. ncbi The deubiquitinating enzyme Doa4p protects cells from DNA topoisomerase I poisons
    Paola Fiorani
    Department of Molecular Pharmacology, St Jude Children s Research Hospital, 332 N Lauderdale, Memphis, TN 38105, USA
    J Biol Chem 279:21271-81. 2004
    ..Additional studies suggest a role for Doa4p in the Rad9p checkpoint response to Top1p poisons. These findings indicate a functional link between ubiquitin-mediated proteolysis and cellular resistance to CPT-induced DNA damage...