VPS35

Summary

Gene Symbol: VPS35
Description: retromer subunit VPS35
Alias: GRD9, VPT7, retromer subunit VPS35
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Nothwehr S, Bryant N, Stevens T. The newly identified yeast GRD genes are required for retention of late-Golgi membrane proteins. Mol Cell Biol. 1996;16:2700-7 pubmed
    ..All of the grd mutants exhibited significant mislocalization of A-ALP, as measured by determining the kinetics of A-ALP processing and by analyzing its ..
  2. Zhao X, Nothwehr S, Lara Lemus R, Zhang B, Peter H, Arvan P. Dominant-negative behavior of mammalian Vps35 in yeast requires a conserved PRLYL motif involved in retromer assembly. Traffic. 2007;8:1829-40 pubmed
    ..Yeast Vps35p (yVps35) exhibits similarity to human Vps35 (hVps35), especially in a completely conserved PRLYL motif contained within an amino-terminal domain...
  3. Seaman M, McCaffery J, Emr S. A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast. J Cell Biol. 1998;142:665-81 pubmed
    ..Conservation of the yeast retromer complex components in higher eukaryotes suggests an important general role for this complex in endosome-to-Golgi retrieval. ..
  4. Reddy J, Seaman M. Vps26p, a component of retromer, directs the interactions of Vps35p in endosome-to-Golgi retrieval. Mol Biol Cell. 2001;12:3242-56 pubmed
    ..These domain swap experiments have shown that Vps26p promotes the interactions between the cargo-selective component Vps35p and the structural components Vps5p/Vps17p. ..
  5. Restrepo R, Zhao X, Peter H, Zhang B, Arvan P, Nothwehr S. Structural features of vps35p involved in interaction with other subunits of the retromer complex. Traffic. 2007;8:1841-53 pubmed
    ..In this study, we set out to generate a panel of vps35 dominant-negative mutants that disrupt retromer-mediated cargo sorting...
  6. Conibear E, Stevens T. Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi. Mol Biol Cell. 2000;11:305-23 pubmed
    ..The Vps52/53/54 complex joins a growing list of distinct multisubunit complexes that regulate membrane-trafficking events. ..
  7. Nothwehr S, Ha S, Bruinsma P. Sorting of yeast membrane proteins into an endosome-to-Golgi pathway involves direct interaction of their cytosolic domains with Vps35p. J Cell Biol. 2000;151:297-310 pubmed
    ..were identified that suppressed cargo-specific mutations in Vps35p but did not suppress the retrieval defects of a vps35 null mutation. Suppression was shown to be due to an improvement in protein sorting at the prevacuolar compartment...
  8. Gokool S, Tattersall D, Reddy J, Seaman M. Identification of a conserved motif required for Vps35p/Vps26p interaction and assembly of the retromer complex. Biochem J. 2007;408:287-95 pubmed
    ..The recognition of cargo proteins by the retromer is performed by the Vps35p/VPS35 (where Vps is vacuolar protein sorting) component, which together with Vps26p/VPS26 and Vps29p/VPS29, forms the ..
  9. Liu T, Gomez T, Sackey B, Billadeau D, Burd C. Rab GTPase regulation of retromer-mediated cargo export during endosome maturation. Mol Biol Cell. 2012;23:2505-15 pubmed publisher
    The retromer complex, composed of sorting nexin subunits and a Vps26/Vps29/Vps35 trimer, mediates sorting of retrograde cargo from the endosome to the trans-Golgi network...

More Information

Publications21

  1. Deloche O, Schekman R. Vps10p cycles between the TGN and the late endosome via the plasma membrane in clathrin mutants. Mol Biol Cell. 2002;13:4296-307 pubmed
    ..Once relieved of cargo protein, Vps10p would be recycled to the trans-Golgi and then to the cell surface for further rounds of sorting. ..
  2. Shi Y, Stefan C, Rue S, Teis D, Emr S. Two novel WD40 domain-containing proteins, Ere1 and Ere2, function in the retromer-mediated endosomal recycling pathway. Mol Biol Cell. 2011;22:4093-107 pubmed publisher
    ..Moreover, a complex containing the two novel proteins Ere1 and Ere2 mediates cargo-specific recognition by the retromer pathway. ..
  3. Luo W, Chang A. Novel genes involved in endosomal traffic in yeast revealed by suppression of a targeting-defective plasma membrane ATPase mutant. J Cell Biol. 1997;138:731-46 pubmed
    ..Thus, cell surface delivery of mutant Pma1 can occur as a consequence of disturbances at several different sites in the endosomal system. ..
  4. Arcones I, Sacristan C, Roncero C. Maintaining protein homeostasis: early and late endosomal dual recycling for the maintenance of intracellular pools of the plasma membrane protein Chs3. Mol Biol Cell. 2016;27:4021-4032 pubmed
  5. Kama R, Robinson M, Gerst J. Btn2, a Hook1 ortholog and potential Batten disease-related protein, mediates late endosome-Golgi protein sorting in yeast. Mol Cell Biol. 2007;27:605-21 pubmed
    ..g., Snc1 and Snc2 [Snc1/2], Tlg1, Tlg2, and Vti1), the Snx4 sorting nexin, and retromer (e.g., Vps26 and Vps35)...
  6. Luo Z, Gallwitz D. Biochemical and genetic evidence for the involvement of yeast Ypt6-GTPase in protein retrieval to different Golgi compartments. J Biol Chem. 2003;278:791-9 pubmed
    ..The results obtained indicate that Ypt6p acts in endosome-to-Golgi, in intra-Golgi retrograde transport, and possibly also in Golgi-to-ER trafficking. ..
  7. Takahashi M, Frost C, Oyadomari K, Pinho M, Sao D, Chima Okereke O, et al. A novel immunodetection screen for vacuolar defects identifies a unique allele of VPS35 in S. cerevisiae. Mol Cell Biochem. 2008;311:121-36 pubmed publisher
    ..env mutants define five complementation groups. The first gene of the collection to be cloned, ENV1 is allelic to VPS35 whose established function is in retrograde trafficking from late endosome to trans-Golgi network (TGN)...
  8. Dechamps C, Portetelle D, Vandenbol M. Multicopy suppression screen in the msb3 msb4 Saccharomyces cerevisiae double mutant, affected in Ypt/RabGAP activity. Biotechnol Lett. 2005;27:1439-49 pubmed
  9. Bean B, Davey M, Snider J, Jessulat M, Deineko V, Tinney M, et al. Rab5-family guanine nucleotide exchange factors bind retromer and promote its recruitment to endosomes. Mol Biol Cell. 2015;26:1119-28 pubmed publisher
    ..The interaction of retromer with distinct VPS9 GEFs could thus link GEF-dependent regulatory inputs to the temporal or spatial coordination of retromer assembly or function. ..
  10. Cui T, Peterson T, Burd C. A CDC25 family protein phosphatase gates cargo recognition by the Vps26 retromer subunit. elife. 2017;6: pubmed publisher
  11. Webster M, McCaffery J, COHEN FIX O. Vesicle trafficking maintains nuclear shape in Saccharomyces cerevisiae during membrane proliferation. J Cell Biol. 2010;191:1079-88 pubmed publisher
    ..Moreover, flares may facilitate maintenance of a constant nuclear/cell volume ratio in the face of altered membrane proliferation. ..
  12. Collins B, Skinner C, Watson P, Seaman M, Owen D. Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly. Nat Struct Mol Biol. 2005;12:594-602 pubmed